Restriction map for
pFN21AE5146
(Circular) (Six-base) MAPSORT of: pFN21AE5146.seq Check: 2480 from: 1 to: 5119
pFN21AE5146 5119 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2929 278
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2229 2462 1937
Size: 292 233 4594
Fragments arranged by size:
4594 292 233
Acc65I G'GTAC_C
Cuts at: 2440 2440
Size: 5119
AclI AA'CG_TT
Cuts at: 1563 3246 3619 1563
Size: 1683 373 3063
Fragments arranged by size:
3063 1683 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 3182 4566 4834 1632
Size: 635 915 1384 268 1917
Fragments arranged by size:
1917 1384 915 635 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4922
AflIII A'CryG_T
Cuts at: 1203 2025 2292 4018 4995 1203
Size: 822 267 1726 977 1327
Fragments arranged by size:
1726 1327 977 822 267
AgeI A'CCGG_T
Cuts at: 2315 3950 2315
Size: 1635 3484
AhdI GACnn_n'nnGTC
Cuts at: 2408 3848 2408
Size: 1440 3679
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5119
AlwNI CAG_nnn'CTG
Cuts at: 2152 2862 4434 2152
Size: 710 1572 2837
Fragments arranged by size:
2837 1572 710
ApaLI G'TGCA_C
Cuts at: 3176 4332 3176
Size: 1156 3963
ApoI r'AATT_y
Cuts at: 1493 2428 2654 2806 4915 1493
Size: 935 226 152 2109 1697
Fragments arranged by size:
2109 1697 935 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3673 160
Size: 872 2641 1606
Fragments arranged by size:
2641 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2444 1870
Size: 73 180 321 4545
Fragments arranged by size:
4545 321 180 73
BamHI G'GATC_C
Cuts at: 2449 2816 2449
Size: 367 4752
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2158 2440 3895 618
Size: 325 217 627 371 282 1455 1842
Fragments arranged by size:
1842 1455 627 371 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2438 1391
Size: 1047 4072
BbeI G_GCGC'C
Cuts at: 2162 2162
Size: 5119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2255 928
Size: 971 356 3792
Fragments arranged by size:
3792 971 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3310 3344 1903
Size: 34 50 34 1289 34 3678
Fragments arranged by size:
3678 1289 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3011 4221 3011
Size: 1210 3909
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5119
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3730 136
Size: 107 122 71 631 605 2058 1525
Fragments arranged by size:
2058 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5114 5114
Size: 5119
BlpI GC'TnA_GC
Cuts at: 2536 2536
Size: 5119
Bme1580I G_kGCm'C
Cuts at: 1790 3180 4336 1790
Size: 1390 1156 2573
Fragments arranged by size:
2573 1390 1156
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 4210
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2850 3808 442
Size: 416 53 83 399 24 1433 958 1753
Fragments arranged by size:
1753 1433 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3779 1239
Size: 875 1665 2579
Fragments arranged by size:
2579 1665 875
Bpu10I CC'TnA_GC
Cuts at: 2048 2048
Size: 5119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 3244 4109 4407 2321
Size: 923 865 298 3033
Fragments arranged by size:
3033 923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2090 3782 882
Size: 617 591 1692 2219
Fragments arranged by size:
2219 1692 617 591
BsaAI yAC'GTr
Cuts at: 493 1204 2293 2910 493
Size: 711 1089 617 2702
Fragments arranged by size:
2702 1089 711 617
BsaBI GATnn'nnATC
Cuts at: 5113 5113
Size: 5119
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2159 2926 3308 3943
Size: 53 83 186 936 626 767 382 635
Cuts at: 3943 275
Size: 1451
Fragments arranged by size:
1451 936 767 635 626 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2254 2315 3552 3950 4224 4371 4782 2254
Size: 61 1237 398 274 147 411 2591
Fragments arranged by size:
2591 1237 411 398 274 147 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2216 2246 1178
Size: 30 165 30 813 30 4051
Fragments arranged by size:
4051 813 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2224 1186
Size: 940 98 4081
Fragments arranged by size:
4081 940 98
BseYI C'CCAG_C
Cuts at: 2134 2152 4322 4818 2134
Size: 18 2170 496 2435
Fragments arranged by size:
2435 2170 496 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2400 2569 3330 4358 1540
Size: 275 186 399 169 761 1028 2301
Fragments arranged by size:
2301 1028 761 399 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 3180 3265 4336 3180
Size: 85 1071 3963
Fragments arranged by size:
3963 1071 85
BsmI GAATG_Cn'
Cuts at: 2630 2723 2630
Size: 93 5026
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2438 3180 3265 4336 1391
Size: 399 648 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 399 85
BspEI T'CCGG_A
Cuts at: 4782 4782
Size: 5119
BspHI T'CATG_A
Cuts at: 3008 4738 3008
Size: 1730 3389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2474 844
Size: 1630 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3006 1394
Size: 429 1183 3507
Fragments arranged by size:
3507 1183 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3614 3788 65
Size: 1183 2366 174 1396
Fragments arranged by size:
2366 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2315 3763 3950 1700
Size: 110 505 1448 187 2869
Fragments arranged by size:
2869 1448 505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5119
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5119
BssSI C'ACGA_G
Cuts at: 3179 4191 3179
Size: 1012 4107
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2199 794
Size: 1405 3714
BstBI TT'CG_AA
Cuts at: 3923 3923
Size: 5119
BstEII G'GTnAC_C
Cuts at: 1184 2318 1184
Size: 1134 3985
BstYI r'GATC_y
Cuts at: 1972 2449 2816 3201 3218 4670 4756 4768 5114
Size: 477 367 385 17 1452 86 12 346
Cuts at: 5114 1972
Size: 1977
Fragments arranged by size:
1977 1452 477 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2282 2282
Size: 5119
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4567
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4380
Fragments arranged by size:
4380 683 56
BtsI GCAGTG_nn'
Cuts at: 2635 3429 3449 2635
Size: 794 20 4305
Fragments arranged by size:
4305 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5084
DraI TTT'AAA
Cuts at: 2423 2423
Size: 5119
DraIII CAC_nnn'GTG
Cuts at: 2293 2293
Size: 5119
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4126 809
Size: 507 2810 1802
Fragments arranged by size:
2810 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2359 2397 2566
Size: 54 1425 50 275 18 529 38 169
Cuts at: 2566 3455 5084 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 529 275 169 54 50
43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2397 2566 1537
Size: 275 585 169 4090
Fragments arranged by size:
4090 585 275 169
EarI CTCTTCn'nnn_
Cuts at: 1353 3049 1353
Size: 1696 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3706 4080 4226 387
Size: 1064 138 2117 374 146 1280
Fragments arranged by size:
2117 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2267 3182 3779 4566 4834 1239
Size: 393 482 153 915 597 787 268 1524
Fragments arranged by size:
1524 915 787 597 482 393 268 153
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2051
Size: 5119
EcoO109I rG'GnC_Cy
Cuts at: 2176 2184 2176
Size: 8 5111
EcoRI G'AATT_C
Cuts at: 2428 2806 2428
Size: 378 4741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 5119
FspI TGC'GCA
Cuts at: 3625 3625
Size: 5119
HaeII r_GCGC'y
Cuts at: 1678 2069 2162 4266 1678
Size: 391 93 2104 2531
Fragments arranged by size:
2531 2104 391 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2062 2094 2394 2426 3760
Size: 32 135 32 490 32 300 32 1334
Cuts at: 3760 3792 3834 3866 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1334 490 300 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2463 2709 669
Size: 988 281 525 246 3079
Fragments arranged by size:
3079 988 525 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5119
HpaI GTT'AAC
Cuts at: 2709 2709
Size: 5119
KasI G'GCGC_C
Cuts at: 2158 2158
Size: 5119
KpnI G_GTAC'C
Cuts at: 2444 2444
Size: 5119
MfeI C'AATT_G
Cuts at: 2718 2718
Size: 5119
MluI A'CGCG_T
Cuts at: 4995 4995
Size: 5119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4233 4417 1305
Size: 2928 184 2007
Fragments arranged by size:
2928 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2361 10
Size: 54 1425 343 529 2768
Fragments arranged by size:
2768 1425 529 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3077 3436 3595 518
Size: 648 1911 359 159 2042
Fragments arranged by size:
2042 1911 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2134 2245 2385 2535 3212 4360 4605
Size: 405 123 111 140 150 677 1148 245
Cuts at: 4605 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 405 245 150 140 123
111
NarI GG'CG_CC
Cuts at: 2159 2159
Size: 5119
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4567
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5119
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5119
NotI GC'GGCC_GC
Cuts at: 2566 2566
Size: 5119
NspI r_CATG'y
Cuts at: 1132 1229 2029 2477 4022 1132
Size: 97 800 448 1545 2229
Fragments arranged by size:
2229 1545 800 448 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5119
PciI A'CATG_T
Cuts at: 2025 4018 2025
Size: 1993 3126
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5119
PmeI GTTT'AAAC
Cuts at: 2423 2423
Size: 5119
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 4701 4733 3188
Size: 32 1481 32 3574
Fragments arranged by size:
3574 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2184 2184
Size: 5119
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5119
PsiI TTA'TAA
Cuts at: 2689 2689
Size: 5119
PstI C_TGCA'G
Cuts at: 830 2471 830
Size: 1641 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5119
PvuII CAG'CTG
Cuts at: 2011 2245 2011
Size: 234 4885
SalI G'TCGA_C
Cuts at: 1936 2461 1936
Size: 525 4594
SanDI GG'GwC_CC
Cuts at: 2184 2184
Size: 5119
SbfI CC_TGCA'GG
Cuts at: 2471 2471
Size: 5119
ScaI AGT'ACT
Cuts at: 2342 3367 5079 2342
Size: 1025 1712 2382
Fragments arranged by size:
2382 1712 1025
SfcI C'TryA_G
Cuts at: 826 1045 2467 3602 4283 4474 826
Size: 219 1422 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2160 2160
Size: 5119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
SgrAI Cr'CCGG_yG
Cuts at: 1700 2315 1700
Size: 615 4504
SmaI CCC'GGG
Cuts at: 1872 2125 2446 1872
Size: 253 321 4545
Fragments arranged by size:
4545 321 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 3223 4124 4386 820
Size: 197 926 393 887 901 262 1553
Fragments arranged by size:
1553 926 901 887 393 262 197
SnaBI TAC'GTA
Cuts at: 493 2910 493
Size: 2417 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5119
SphI G_CATG'C
Cuts at: 1132 2477 1132
Size: 1345 3774
SspI AAT'ATT
Cuts at: 5 52 3043 5
Size: 47 2991 2081
Fragments arranged by size:
2991 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5119
StyI C'CwwG_G
Cuts at: 513 1065 2558 513
Size: 552 1493 3074
Fragments arranged by size:
3074 1493 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3146 3163 3316 3501 3946 325
Size: 1255 1566 17 153 185 445 1498
Fragments arranged by size:
1566 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2340 3365 5077
Size: 274 80 33 51 1443 363 1025 1712
Cuts at: 5077 96
Size: 138
Fragments arranged by size:
1712 1443 1025 363 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 5079 5111 2216
Size: 32 2831 32 2224
Fragments arranged by size:
2831 2224 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5119
XbaI T'CTAG_A
Cuts at: 2455 2455
Size: 5119
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2176
Size: 5119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5119
XmaI C'CCGG_G
Cuts at: 1870 2123 2444 1870
Size: 253 321 4545
Fragments arranged by size:
4545 321 253
XmnI GAAnn'nnTTC
Cuts at: 3248 3248
Size: 5119
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2927 276
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI