Restriction map for
pFN21AE5145
(Circular) (Six-base) MAPSORT of: pFN21AE5145.seq Check: 994 from: 1 to: 5005
pFN21AE5145 5005 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2057 2057
Size: 5005
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2815 278
Size: 53 83 186 936 1279 2468
Fragments arranged by size:
2468 1279 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2348 1937
Size: 411 4594
Acc65I G'GTAC_C
Cuts at: 2326 2326
Size: 5005
AclI AA'CG_TT
Cuts at: 1563 3132 3505 1563
Size: 1569 373 3063
Fragments arranged by size:
3063 1569 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3068 4452 4720 1632
Size: 1436 1384 268 1917
Fragments arranged by size:
1917 1436 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4808
AflIII A'CryG_T
Cuts at: 1203 3904 4881 1203
Size: 2701 977 1327
Fragments arranged by size:
2701 1327 977
AgeI A'CCGG_T
Cuts at: 3836 3836
Size: 5005
AhdI GACnn_n'nnGTC
Cuts at: 3734 3734
Size: 5005
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5005
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5005
AlwNI CAG_nnn'CTG
Cuts at: 2748 4320 2748
Size: 1572 3433
ApaLI G'TGCA_C
Cuts at: 3062 4218 3062
Size: 1156 3849
ApoI r'AATT_y
Cuts at: 1493 2314 2540 2692 4801 1493
Size: 821 226 152 2109 1697
Fragments arranged by size:
2109 1697 821 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3559 160
Size: 872 2527 1606
Fragments arranged by size:
2527 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5005
AvaI C'yCGr_G
Cuts at: 1870 1943 2330 1870
Size: 73 387 4545
Fragments arranged by size:
4545 387 73
BamHI G'GATC_C
Cuts at: 2197 2335 2702 2197
Size: 138 367 4500
Fragments arranged by size:
4500 367 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 3781 618
Size: 325 217 627 539 1455 1842
Fragments arranged by size:
1842 1455 627 539 325 217
BanII G_rGCy'C
Cuts at: 1391 2014 2182 2211 2232 2324 1391
Size: 623 168 29 21 92 4072
Fragments arranged by size:
4072 623 168 92 29 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3196 3230 1903
Size: 34 1259 34 3678
Fragments arranged by size:
3678 1259 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2897 4107 2897
Size: 1210 3795
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5005
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 3616 136
Size: 107 122 71 631 605 361 1583 1525
Fragments arranged by size:
1583 1525 631 605 361 122 107 71
BglII A'GATC_T
Cuts at: 5000 5000
Size: 5005
BlpI GC'TnA_GC
Cuts at: 2422 2422
Size: 5005
Bme1580I G_kGCm'C
Cuts at: 1790 3066 4222 1790
Size: 1276 1156 2573
Fragments arranged by size:
2573 1276 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5005
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2177 2736 3694
Size: 416 53 83 399 24 760 559 958
Cuts at: 3694 442
Size: 1753
Fragments arranged by size:
1753 958 760 559 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 3665 1239
Size: 986 1440 2579
Fragments arranged by size:
2579 1440 986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3130 3995 4293 3130
Size: 865 298 3842
Fragments arranged by size:
3842 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 3668 882
Size: 617 641 1528 2219
Fragments arranged by size:
2219 1528 641 617
BsaAI yAC'GTr
Cuts at: 493 1204 2796 493
Size: 711 1592 2702
Fragments arranged by size:
2702 1592 711
BsaBI GATnn'nnATC
Cuts at: 4999 4999
Size: 5005
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2812 3194 3829 275
Size: 53 83 186 936 1279 382 635 1451
Fragments arranged by size:
1451 1279 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2236 3438 3836 4110 4257 4668 2236
Size: 1202 398 274 147 411 2573
Fragments arranged by size:
2573 1202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2169 2199 1178
Size: 30 165 30 766 30 3984
Fragments arranged by size:
3984 766 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 1186
Size: 826 4179
BseYI C'CCAG_C
Cuts at: 2078 2173 4208 4704 2078
Size: 95 2035 496 2379
Fragments arranged by size:
2379 2035 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2106 2124 1163
Size: 573 370 18 4044
Fragments arranged by size:
4044 573 370 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2304 2455 3216 4244 1540
Size: 275 186 303 151 761 1028 2301
Fragments arranged by size:
2301 1028 761 303 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2232 3066 3151 4222 2232
Size: 834 85 1071 3015
Fragments arranged by size:
3015 1071 834 85
BsmI GAATG_Cn'
Cuts at: 2516 2609 2516
Size: 93 4912
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2182 2211 2232 2324 3066 3151
Size: 399 224 168 29 21 92 742 85
Cuts at: 3151 4222 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 224 168 92 85
29 21
BspEI T'CCGG_A
Cuts at: 2236 4668 2236
Size: 2432 2573
BspHI T'CATG_A
Cuts at: 2894 4624 2894
Size: 1730 3275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2360 844
Size: 1213 303 3489
Fragments arranged by size:
3489 1213 303
BsrBI CCG'CTC
Cuts at: 1394 1823 2892 1394
Size: 429 1069 3507
Fragments arranged by size:
3507 1069 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3500 3674 65
Size: 1183 2252 174 1396
Fragments arranged by size:
2252 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3649 3836 1700
Size: 110 1839 187 2869
Fragments arranged by size:
2869 1839 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5005
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5005
BssSI C'ACGA_G
Cuts at: 3065 4077 3065
Size: 1012 3993
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5005
BstBI TT'CG_AA
Cuts at: 3809 3809
Size: 5005
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5005
BstYI r'GATC_y
Cuts at: 1972 2197 2335 2702 3087 3104 4556 4642 4654
Size: 225 138 367 385 17 1452 86 12
Cuts at: 4654 5000 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 225 138 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 487
Size: 683 56 812 3454
Fragments arranged by size:
3454 812 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 3315 3335 2521
Size: 794 20 4191
Fragments arranged by size:
4191 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5005
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4970
DraI TTT'AAA
Cuts at: 2309 2309
Size: 5005
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4012 809
Size: 507 2696 1802
Fragments arranged by size:
2696 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2059 2452
Size: 54 1425 50 275 18 210 19 393
Cuts at: 2452 3341 4970 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 393 275 210 54 50
43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2452 1537
Size: 275 640 4090
Fragments arranged by size:
4090 640 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 2935 1353
Size: 785 797 3423
Fragments arranged by size:
3423 797 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3592 3966 4112 387
Size: 1064 138 2003 374 146 1280
Fragments arranged by size:
2003 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2230 2230
Size: 5005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 3068 3665 4452 4720 1239
Size: 393 593 843 597 787 268 1524
Fragments arranged by size:
1524 843 787 597 593 393 268
EcoRI G'AATT_C
Cuts at: 2314 2692 2314
Size: 378 4627
FspI TGC'GCA
Cuts at: 3511 3511
Size: 5005
HaeII r_GCGC'y
Cuts at: 1678 2158 2290 4152 1678
Size: 480 132 1862 2531
Fragments arranged by size:
2531 1862 480 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2169 2201 3646 3678 3720
Size: 32 135 32 597 32 1445 32 42
Cuts at: 3720 3752 1373
Size: 32 2626
Fragments arranged by size:
2626 1445 597 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2349 2595 669
Size: 988 281 411 246 3079
Fragments arranged by size:
3079 988 411 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5005
HpaI GTT'AAC
Cuts at: 2595 2595
Size: 5005
KpnI G_GTAC'C
Cuts at: 2330 2330
Size: 5005
MfeI C'AATT_G
Cuts at: 2604 2604
Size: 5005
MluI A'CGCG_T
Cuts at: 4881 4881
Size: 5005
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4119 4303 1305
Size: 2814 184 2007
Fragments arranged by size:
2814 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2061 10
Size: 54 1425 343 229 2954
Fragments arranged by size:
2954 1425 343 229 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2963 3322 3481 518
Size: 648 1797 359 159 2042
Fragments arranged by size:
2042 1797 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2421 3098 4246 4491 1606
Size: 476 339 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 476 339 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4453
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5005
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5005
NotI GC'GGCC_GC
Cuts at: 2452 2452
Size: 5005
NspI r_CATG'y
Cuts at: 1132 1229 2363 3908 1132
Size: 97 1134 1545 2229
Fragments arranged by size:
2229 1545 1134 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5005
PciI A'CATG_T
Cuts at: 3904 3904
Size: 5005
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5005
PmeI GTTT'AAAC
Cuts at: 2309 2309
Size: 5005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 4587 4619 3074
Size: 32 1481 32 3460
Fragments arranged by size:
3460 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5005
PsiI TTA'TAA
Cuts at: 2575 2575
Size: 5005
PstI C_TGCA'G
Cuts at: 830 2357 830
Size: 1527 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5005
SacI G_AGCT'C
Cuts at: 2232 2232
Size: 5005
SalI G'TCGA_C
Cuts at: 1936 2347 1936
Size: 411 4594
SbfI CC_TGCA'GG
Cuts at: 2357 2357
Size: 5005
ScaI AGT'ACT
Cuts at: 3253 4965 3253
Size: 1712 3293
SfcI C'TryA_G
Cuts at: 826 1045 2353 3488 4169 4360 826
Size: 219 1308 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5005
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5005
SmaI CCC'GGG
Cuts at: 1872 2332 1872
Size: 460 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3109 4010 4272 820
Size: 197 926 1166 901 262 1553
Fragments arranged by size:
1553 1166 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2796 493
Size: 2303 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5005
SphI G_CATG'C
Cuts at: 1132 2363 1132
Size: 1231 3774
SspI AAT'ATT
Cuts at: 5 52 2929 5
Size: 47 2877 2081
Fragments arranged by size:
2877 2081 47
StuI AGG'CCT
Cuts at: 1517 2279 1517
Size: 762 4243
StyI C'CwwG_G
Cuts at: 513 1065 2444 513
Size: 552 1379 3074
Fragments arranged by size:
3074 1379 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3032 3049 3202 3387 3832 325
Size: 1255 1452 17 153 185 445 1498
Fragments arranged by size:
1498 1452 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3251 4963 96
Size: 274 80 33 51 1443 1274 1712 138
Fragments arranged by size:
1712 1443 1274 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4965 4997 4965
Size: 32 4973
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5005
XbaI T'CTAG_A
Cuts at: 2341 2341
Size: 5005
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5005
XmaI C'CCGG_G
Cuts at: 1870 2330 1870
Size: 460 4545
XmnI GAAnn'nnTTC
Cuts at: 3134 3134
Size: 5005
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2813 276
Size: 53 83 186 936 1279 2468
Fragments arranged by size:
2468 1279 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI