Restriction map for
pFN21AE5143
(Circular) (Six-base) MAPSORT of: pFN21AE5143.seq Check: 3414 from: 1 to: 4936
pFN21AE5143 4936 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2746 278
Size: 53 83 186 936 1210 2468
Fragments arranged by size:
2468 1210 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2279 1937
Size: 342 4594
Acc65I G'GTAC_C
Cuts at: 2257 2257
Size: 4936
AclI AA'CG_TT
Cuts at: 1563 3063 3436 1563
Size: 1500 373 3063
Fragments arranged by size:
3063 1500 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2999 4383 4651 1632
Size: 1367 1384 268 1917
Fragments arranged by size:
1917 1384 1367 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4739
AflIII A'CryG_T
Cuts at: 1203 3835 4812 1203
Size: 2632 977 1327
Fragments arranged by size:
2632 1327 977
AgeI A'CCGG_T
Cuts at: 3767 3767
Size: 4936
AhdI GACnn_n'nnGTC
Cuts at: 3665 3665
Size: 4936
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4936
AlwNI CAG_nnn'CTG
Cuts at: 2108 2679 4251 2108
Size: 571 1572 2793
Fragments arranged by size:
2793 1572 571
ApaLI G'TGCA_C
Cuts at: 2993 4149 2993
Size: 1156 3780
ApoI r'AATT_y
Cuts at: 1493 2245 2471 2623 4732 1493
Size: 752 226 152 2109 1697
Fragments arranged by size:
2109 1697 752 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3490 160
Size: 872 2458 1606
Fragments arranged by size:
2458 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4936
AvaI C'yCGr_G
Cuts at: 1870 1943 2261 1870
Size: 73 318 4545
Fragments arranged by size:
4545 318 73
BamHI G'GATC_C
Cuts at: 2266 2633 2266
Size: 367 4569
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2257 3712 618
Size: 325 217 627 470 1455 1842
Fragments arranged by size:
1842 1455 627 470 325 217
BanII G_rGCy'C
Cuts at: 1391 2255 1391
Size: 864 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3965
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3127 3161 1903
Size: 34 1190 34 3678
Fragments arranged by size:
3678 1190 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 2828 4038 2002
Size: 826 1210 2900
Fragments arranged by size:
2900 1210 826
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4936
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3547 136
Size: 107 122 71 631 605 1875 1525
Fragments arranged by size:
1875 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4931 4931
Size: 4936
BlpI GC'TnA_GC
Cuts at: 2353 2353
Size: 4936
Bme1580I G_kGCm'C
Cuts at: 1790 2997 4153 1790
Size: 1207 1156 2573
Fragments arranged by size:
2573 1207 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4936
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2667 3625 442
Size: 416 53 83 399 24 1250 958 1753
Fragments arranged by size:
1753 1250 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3596 1239
Size: 2357 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3061 3926 4224 3061
Size: 865 298 3773
Fragments arranged by size:
3773 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3599 882
Size: 617 2100 2219
Fragments arranged by size:
2219 2100 617
BsaAI yAC'GTr
Cuts at: 493 1204 2727 493
Size: 711 1523 2702
Fragments arranged by size:
2702 1523 711
BsaBI GATnn'nnATC
Cuts at: 4930 4930
Size: 4936
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2743 3125 3760 275
Size: 53 83 186 936 1210 382 635 1451
Fragments arranged by size:
1451 1210 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3369 3767 4041 4188 4599 3369
Size: 398 274 147 411 3706
Fragments arranged by size:
3706 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4711
Fragments arranged by size:
4711 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 1186
Size: 859 4077
BseYI C'CCAG_C
Cuts at: 4139 4635 4139
Size: 496 4440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2386 3147 4175 1540
Size: 275 186 385 761 1028 2301
Fragments arranged by size:
2301 1028 761 385 275 186
BsiHKAI G_wGCw'C
Cuts at: 2997 3082 4153 2997
Size: 85 1071 3780
Fragments arranged by size:
3780 1071 85
BsmI GAATG_Cn'
Cuts at: 2447 2540 2447
Size: 93 4843
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2997 3082 4153 1391
Size: 399 465 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 465 399 85
BspEI T'CCGG_A
Cuts at: 4599 4599
Size: 4936
BspHI T'CATG_A
Cuts at: 2203 2825 4555 2203
Size: 622 1730 2584
Fragments arranged by size:
2584 1730 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2291 844
Size: 1447 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2823 1394
Size: 429 1000 3507
Fragments arranged by size:
3507 1000 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3431 3605 65
Size: 1183 2183 174 1396
Fragments arranged by size:
2183 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3580 3767 1700
Size: 110 1770 187 2869
Fragments arranged by size:
2869 1770 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4936
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4936
BssSI C'ACGA_G
Cuts at: 2996 4008 2996
Size: 1012 3924
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4936
BstBI TT'CG_AA
Cuts at: 3740 3740
Size: 4936
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4936
BstYI r'GATC_y
Cuts at: 1972 2266 2633 3018 3035 4487 4573 4585 4931
Size: 294 367 385 17 1452 86 12 346
Cuts at: 4931 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 294 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3445
Fragments arranged by size:
3445 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4197
Fragments arranged by size:
4197 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 2452 3246 3266 2109
Size: 343 794 20 3779
Fragments arranged by size:
3779 794 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4901
DraI TTT'AAA
Cuts at: 2240 2240
Size: 4936
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3943 809
Size: 507 2627 1802
Fragments arranged by size:
2627 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 2383 3272
Size: 54 1425 50 275 18 364 189 889
Cuts at: 3272 4901 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 364 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2383 1537
Size: 275 571 4090
Fragments arranged by size:
4090 571 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2866 1353
Size: 1513 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3523 3897 4043 387
Size: 1064 138 1934 374 146 1280
Fragments arranged by size:
1934 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2999 3596 4383 4651 1239
Size: 393 1367 597 787 268 1524
Fragments arranged by size:
1524 1367 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2245 2623 2245
Size: 378 4558
FspI TGC'GCA
Cuts at: 3442 3442
Size: 4936
HaeII r_GCGC'y
Cuts at: 1678 2030 2048 4083 1678
Size: 352 18 2035 2531
Fragments arranged by size:
2531 2035 352 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3577 3609 3651 3683 1373
Size: 32 135 32 2005 32 42 32 2626
Fragments arranged by size:
2626 2005 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2280 2526 669
Size: 988 281 342 246 3079
Fragments arranged by size:
3079 988 342 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4936
HpaI GTT'AAC
Cuts at: 2526 2526
Size: 4936
KpnI G_GTAC'C
Cuts at: 2261 2261
Size: 4936
MfeI C'AATT_G
Cuts at: 2535 2535
Size: 4936
MluI A'CGCG_T
Cuts at: 4812 4812
Size: 4936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4050 4234 1305
Size: 2745 184 2007
Fragments arranged by size:
2745 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2196 10
Size: 54 1425 343 364 2750
Fragments arranged by size:
2750 1425 364 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2894 3253 3412 518
Size: 648 1728 359 159 2042
Fragments arranged by size:
2042 1728 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2352 3029 4177 4422 1606
Size: 746 677 1148 245 2120
Fragments arranged by size:
2120 1148 746 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3445
Fragments arranged by size:
3445 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4936
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4936
NotI GC'GGCC_GC
Cuts at: 2383 2383
Size: 4936
NspI r_CATG'y
Cuts at: 1132 1229 2294 3839 1132
Size: 97 1065 1545 2229
Fragments arranged by size:
2229 1545 1065 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4936
PciI A'CATG_T
Cuts at: 3835 3835
Size: 4936
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4936
PmeI GTTT'AAAC
Cuts at: 2240 2240
Size: 4936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 4518 4550 3005
Size: 32 1481 32 3391
Fragments arranged by size:
3391 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4936
PsiI TTA'TAA
Cuts at: 2506 2506
Size: 4936
PstI C_TGCA'G
Cuts at: 830 2288 830
Size: 1458 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4936
SalI G'TCGA_C
Cuts at: 1936 2278 1936
Size: 342 4594
SbfI CC_TGCA'GG
Cuts at: 2288 2288
Size: 4936
ScaI AGT'ACT
Cuts at: 3184 4896 3184
Size: 1712 3224
SfcI C'TryA_G
Cuts at: 826 1045 2284 3419 4100 4291 826
Size: 219 1239 1135 681 191 1471
Fragments arranged by size:
1471 1239 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4936
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4936
SmaI CCC'GGG
Cuts at: 1872 2263 1872
Size: 391 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3040 3941 4203 820
Size: 197 926 1097 901 262 1553
Fragments arranged by size:
1553 1097 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2727 493
Size: 2234 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4936
SphI G_CATG'C
Cuts at: 1132 2294 1132
Size: 1162 3774
SspI AAT'ATT
Cuts at: 5 52 2860 5
Size: 47 2808 2081
Fragments arranged by size:
2808 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4936
StyI C'CwwG_G
Cuts at: 513 1065 2004 2063 2086 2375 513
Size: 552 939 59 23 289 3074
Fragments arranged by size:
3074 939 552 289 59 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2963 2980 3133 3318 3763 325
Size: 1255 1383 17 153 185 445 1498
Fragments arranged by size:
1498 1383 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 3182 4894
Size: 274 80 33 51 1443 252 953 1712
Cuts at: 4894 96
Size: 138
Fragments arranged by size:
1712 1443 953 274 252 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4896 4928 4896
Size: 32 4904
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4936
XbaI T'CTAG_A
Cuts at: 2272 2272
Size: 4936
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4936
XmaI C'CCGG_G
Cuts at: 1870 2261 1870
Size: 391 4545
XmnI GAAnn'nnTTC
Cuts at: 3065 3065
Size: 4936
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2744 276
Size: 53 83 186 936 1210 2468
Fragments arranged by size:
2468 1210 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI