Restriction map for
pFN21AE5134
(Circular) (Six-base) MAPSORT of: pFN21AE5134.seq Check: 48 from: 1 to: 5200
pFN21AE5134 5200 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3010 278
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2543 1937
Size: 606 4594
Acc65I G'GTAC_C
Cuts at: 2521 2521
Size: 5200
AclI AA'CG_TT
Cuts at: 1563 3327 3700 1563
Size: 1764 373 3063
Fragments arranged by size:
3063 1764 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 3263 4647 4915 1632
Size: 714 917 1384 268 1917
Fragments arranged by size:
1917 1384 917 714 268
AfeI AGC'GCT
Cuts at: 2147 2147
Size: 5200
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5003
AflIII A'CryG_T
Cuts at: 1203 4099 5076 1203
Size: 2896 977 1327
Fragments arranged by size:
2896 1327 977
AgeI A'CCGG_T
Cuts at: 4031 4031
Size: 5200
AhdI GACnn_n'nnGTC
Cuts at: 3929 3929
Size: 5200
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5200
AlwNI CAG_nnn'CTG
Cuts at: 2943 4515 2943
Size: 1572 3628
ApaLI G'TGCA_C
Cuts at: 3257 4413 3257
Size: 1156 4044
ApoI r'AATT_y
Cuts at: 1493 2509 2735 2887 4996 1493
Size: 1016 226 152 2109 1697
Fragments arranged by size:
2109 1697 1016 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3754 160
Size: 872 2722 1606
Fragments arranged by size:
2722 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 1870
Size: 73 582 4545
Fragments arranged by size:
4545 582 73
BamHI G'GATC_C
Cuts at: 2530 2897 2530
Size: 367 4833
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 3976 618
Size: 325 217 627 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2519 1391
Size: 1128 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3391 3425 1903
Size: 34 1454 34 3678
Fragments arranged by size:
3678 1454 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3092 4302 3092
Size: 1210 3990
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5200
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3811 136
Size: 107 122 71 631 605 2139 1525
Fragments arranged by size:
2139 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5195 5195
Size: 5200
BlpI GC'TnA_GC
Cuts at: 2382 2617 2382
Size: 235 4965
Bme1580I G_kGCm'C
Cuts at: 1790 2067 3261 4417 1790
Size: 277 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 277
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 3889 442
Size: 416 53 83 399 24 1514 958 1753
Fragments arranged by size:
1753 1514 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2428 2338
Size: 90 5110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3860 1239
Size: 2621 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3325 4190 4488 3325
Size: 865 298 4037
Fragments arranged by size:
4037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3863 882
Size: 617 2364 2219
Fragments arranged by size:
2364 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2281 2991 493
Size: 711 1077 710 2702
Fragments arranged by size:
2702 1077 711 710
BsaBI GATnn'nnATC
Cuts at: 5194 5194
Size: 5200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3007 3389 4024 275
Size: 53 83 186 936 1474 382 635 1451
Fragments arranged by size:
1474 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3633 4031 4305 4452 4863 3633
Size: 398 274 147 411 3970
Fragments arranged by size:
3970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2226 2256 2358 2388 1178
Size: 30 165 30 823 30 102 30 3990
Fragments arranged by size:
3990 823 165 102 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2486 2489 2501 2504 1186
Size: 1231 69 3 12 3 3882
Fragments arranged by size:
3882 1231 69 12 3 3
BseYI C'CCAG_C
Cuts at: 2377 4403 4899 2377
Size: 2026 496 2678
Fragments arranged by size:
2678 2026 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4627
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2650 3411 4439 1540
Size: 275 186 649 761 1028 2301
Fragments arranged by size:
2301 1028 761 649 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2268 3261 3346 4417 2058
Size: 210 993 85 1071 2841
Fragments arranged by size:
2841 1071 993 210 85
BsmI GAATG_Cn'
Cuts at: 2711 2804 2711
Size: 93 5107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2067 2268 2519 3261 3346 4417
Size: 399 268 9 201 251 742 85 1071
Cuts at: 4417 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 268 251 201 85
9
BspEI T'CCGG_A
Cuts at: 4863 4863
Size: 5200
BspHI T'CATG_A
Cuts at: 2461 3089 4819 2461
Size: 628 1730 2842
Fragments arranged by size:
2842 1730 628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2555 844
Size: 1711 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3087 1394
Size: 429 1264 3507
Fragments arranged by size:
3507 1264 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3695 3869 65
Size: 1183 2447 174 1396
Fragments arranged by size:
2447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3844 4031 1700
Size: 110 2034 187 2869
Fragments arranged by size:
2869 2034 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5200
BssSI C'ACGA_G
Cuts at: 3260 4272 3260
Size: 1012 4188
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2062 794
Size: 1268 3932
BstBI TT'CG_AA
Cuts at: 4004 4004
Size: 5200
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5200
BstYI r'GATC_y
Cuts at: 1972 2530 2897 3282 3299 4751 4837 4849 5195
Size: 558 367 385 17 1452 86 12 346
Cuts at: 5195 1972
Size: 1977
Fragments arranged by size:
1977 1452 558 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2313 2313
Size: 5200
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4648
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4461
Fragments arranged by size:
4461 683 56
BtsI GCAGTG_nn'
Cuts at: 2716 3510 3530 2716
Size: 794 20 4386
Fragments arranged by size:
4386 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5165
DraI TTT'AAA
Cuts at: 2504 2504
Size: 5200
DraIII CAC_nnn'GTG
Cuts at: 2062 2062
Size: 5200
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4207 809
Size: 507 2891 1802
Fragments arranged by size:
2891 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2647 3536 5165
Size: 54 1425 50 275 18 817 889 1629
Cuts at: 5165 8
Size: 43
Fragments arranged by size:
1629 1425 889 817 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2647 1537
Size: 275 835 4090
Fragments arranged by size:
4090 835 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 2478 3130 1353
Size: 870 255 652 3423
Fragments arranged by size:
3423 870 652 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3787 4161 4307 387
Size: 1064 138 2198 374 146 1280
Fragments arranged by size:
2198 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 3263 3860 4647 4915 1239
Size: 393 714 917 597 787 268 1524
Fragments arranged by size:
1524 917 787 714 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2100 2100
Size: 5200
EcoRI G'AATT_C
Cuts at: 2509 2887 2509
Size: 378 4822
FspI TGC'GCA
Cuts at: 3706 3706
Size: 5200
HaeII r_GCGC'y
Cuts at: 1678 2149 4347 1678
Size: 471 2198 2531
Fragments arranged by size:
2531 2198 471
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3841 3873 3915 3947 1373
Size: 32 135 32 2269 32 42 32 2626
Fragments arranged by size:
2626 2269 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2218 2440 2544 2790 669
Size: 988 281 280 222 104 246 3079
Fragments arranged by size:
3079 988 281 280 246 222 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5200
HpaI GTT'AAC
Cuts at: 2440 2790 2440
Size: 350 4850
KpnI G_GTAC'C
Cuts at: 2525 2525
Size: 5200
MfeI C'AATT_G
Cuts at: 2799 2799
Size: 5200
MluI A'CGCG_T
Cuts at: 5076 5076
Size: 5200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 4314 4498 1305
Size: 789 2220 184 2007
Fragments arranged by size:
2220 2007 789 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3378
Fragments arranged by size:
3378 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2466 3158 3517 3676 518
Size: 648 1300 692 359 159 2042
Fragments arranged by size:
2042 1300 692 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2381 2616 3293 4441 4686 1606
Size: 775 235 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 245 235
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4648
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5200
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5200
NotI GC'GGCC_GC
Cuts at: 2647 2647
Size: 5200
NspI r_CATG'y
Cuts at: 1132 1229 2033 2164 2558 4103 1132
Size: 97 804 131 394 1545 2229
Fragments arranged by size:
2229 1545 804 394 131 97
PasI CC'CwG_GG
Cuts at: 1348 2105 1348
Size: 757 4443
PciI A'CATG_T
Cuts at: 4099 4099
Size: 5200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5200
PmeI GTTT'AAAC
Cuts at: 2504 2504
Size: 5200
PmlI CAC'GTG
Cuts at: 2281 2281
Size: 5200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 4782 4814 3269
Size: 32 1481 32 3655
Fragments arranged by size:
3655 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2100
Size: 5200
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5200
PsiI TTA'TAA
Cuts at: 2770 2770
Size: 5200
PstI C_TGCA'G
Cuts at: 830 2295 2552 830
Size: 1465 257 3478
Fragments arranged by size:
3478 1465 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5200
PvuII CAG'CTG
Cuts at: 2381 2381
Size: 5200
SalI G'TCGA_C
Cuts at: 1936 2542 1936
Size: 606 4594
SbfI CC_TGCA'GG
Cuts at: 2552 2552
Size: 5200
ScaI AGT'ACT
Cuts at: 3448 5160 3448
Size: 1712 3488
SfcI C'TryA_G
Cuts at: 826 1045 2291 2548 3683 4364 4555 826
Size: 219 1246 257 1135 681 191 1471
Fragments arranged by size:
1471 1246 1135 681 257 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5200
SmaI CCC'GGG
Cuts at: 1872 2527 1872
Size: 655 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3304 4205 4467 820
Size: 197 926 1361 901 262 1553
Fragments arranged by size:
1553 1361 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2991 493
Size: 2498 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5200
SphI G_CATG'C
Cuts at: 1132 2164 2558 1132
Size: 1032 394 3774
Fragments arranged by size:
3774 1032 394
SspI AAT'ATT
Cuts at: 5 52 3124 5
Size: 47 3072 2081
Fragments arranged by size:
3072 2081 47
StuI AGG'CCT
Cuts at: 1517 2318 1517
Size: 801 4399
StyI C'CwwG_G
Cuts at: 513 1065 2137 2639 513
Size: 552 1072 502 3074
Fragments arranged by size:
3074 1072 552 502
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3227 3244 3397 3582 4027 325
Size: 1255 1647 17 153 185 445 1498
Fragments arranged by size:
1647 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3446 5158 96
Size: 274 80 33 51 1443 1469 1712 138
Fragments arranged by size:
1712 1469 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 5160 5192 2226
Size: 32 2902 32 2234
Fragments arranged by size:
2902 2234 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5200
XbaI T'CTAG_A
Cuts at: 2536 2536
Size: 5200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5200
XmaI C'CCGG_G
Cuts at: 1870 2525 1870
Size: 655 4545
XmnI GAAnn'nnTTC
Cuts at: 3329 3329
Size: 5200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3008 276
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI