Restriction map for
pFN21AE5133
(Circular) (Six-base) MAPSORT of: pFN21AE5133.seq Check: 7018 from: 1 to: 5203
pFN21AE5133 5203 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3013 278
Size: 53 83 186 936 1477 2468
Fragments arranged by size:
2468 1477 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2546 1937
Size: 609 4594
Acc65I G'GTAC_C
Cuts at: 2524 2524
Size: 5203
AclI AA'CG_TT
Cuts at: 1563 3330 3703 1563
Size: 1767 373 3063
Fragments arranged by size:
3063 1767 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 3266 4650 4918 1632
Size: 644 990 1384 268 1917
Fragments arranged by size:
1917 1384 990 644 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5006
AflIII A'CryG_T
Cuts at: 1203 4102 5079 1203
Size: 2899 977 1327
Fragments arranged by size:
2899 1327 977
AgeI A'CCGG_T
Cuts at: 4034 4034
Size: 5203
AhdI GACnn_n'nnGTC
Cuts at: 3932 3932
Size: 5203
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5203
AlwNI CAG_nnn'CTG
Cuts at: 2244 2946 4518 2244
Size: 702 1572 2929
Fragments arranged by size:
2929 1572 702
ApaLI G'TGCA_C
Cuts at: 3260 4416 3260
Size: 1156 4047
ApoI r'AATT_y
Cuts at: 1493 2512 2738 2890 4999 1493
Size: 1019 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3757 160
Size: 872 2725 1606
Fragments arranged by size:
2725 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5203
AvaI C'yCGr_G
Cuts at: 1870 1943 2528 1870
Size: 73 585 4545
Fragments arranged by size:
4545 585 73
BamHI G'GATC_C
Cuts at: 2533 2900 2533
Size: 367 4836
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2524 3979 618
Size: 325 217 627 272 465 1455 1842
Fragments arranged by size:
1842 1455 627 465 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2522 1391
Size: 1131 4072
BbeI G_GCGC'C
Cuts at: 2063 2063
Size: 5203
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3394 3428 1903
Size: 34 1457 34 3678
Fragments arranged by size:
3678 1457 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3095 4305 3095
Size: 1210 3993
BclI T'GATC_A
Cuts at: 1548 2308 1548
Size: 760 4443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3814 136
Size: 107 122 71 631 605 2142 1525
Fragments arranged by size:
2142 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5198 5198
Size: 5203
BlpI GC'TnA_GC
Cuts at: 2620 2620
Size: 5203
Bme1580I G_kGCm'C
Cuts at: 1790 3264 4420 1790
Size: 1474 1156 2573
Fragments arranged by size:
2573 1474 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5203
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2934 3892 442
Size: 416 53 83 399 24 1517 958 1753
Fragments arranged by size:
1753 1517 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2327 1056
Size: 1271 3932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3863 1239
Size: 2624 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3328 4193 4491 3328
Size: 865 298 4040
Fragments arranged by size:
4040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3866 882
Size: 617 2367 2219
Fragments arranged by size:
2367 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2994 493
Size: 711 1790 2702
Fragments arranged by size:
2702 1790 711
BsaBI GATnn'nnATC
Cuts at: 5197 5197
Size: 5203
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 3010 3392 4027
Size: 53 83 186 936 527 950 382 635
Cuts at: 4027 275
Size: 1451
Fragments arranged by size:
1451 950 936 635 527 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2122 3636 4034 4308 4455 4866 2122
Size: 1514 398 274 147 411 2459
Fragments arranged by size:
2459 1514 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4978
Fragments arranged by size:
4978 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5203
BseYI C'CCAG_C
Cuts at: 4406 4902 4406
Size: 496 4707
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 1163
Size: 573 403 4227
Fragments arranged by size:
4227 573 403
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2653 3414 4442 1540
Size: 275 186 652 761 1028 2301
Fragments arranged by size:
2301 1028 761 652 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3264 3349 4420 2012
Size: 1252 85 1071 2795
Fragments arranged by size:
2795 1252 1071 85
BsmI GAATG_Cn'
Cuts at: 2714 2807 2714
Size: 93 5110
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2522 3264 3349 4420 1391
Size: 399 222 510 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 222 85
BspEI T'CCGG_A
Cuts at: 4866 4866
Size: 5203
BspHI T'CATG_A
Cuts at: 3092 4822 3092
Size: 1730 3473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2558 844
Size: 1714 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3090 1394
Size: 429 1267 3507
Fragments arranged by size:
3507 1267 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3698 3872 65
Size: 1183 2450 174 1396
Fragments arranged by size:
2450 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3847 4034 1700
Size: 110 2037 187 2869
Fragments arranged by size:
2869 2037 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5203
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5203
BssSI C'ACGA_G
Cuts at: 3263 4275 3263
Size: 1012 4191
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5203
BstBI TT'CG_AA
Cuts at: 4007 4007
Size: 5203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5203
BstYI r'GATC_y
Cuts at: 1972 2533 2900 3285 3302 4754 4840 4852 5198
Size: 561 367 385 17 1452 86 12 346
Cuts at: 5198 1972
Size: 1977
Fragments arranged by size:
1977 1452 561 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2269 2269
Size: 5203
BtgI C'CryG_G
Cuts at: 513 1065 2013 2063 513
Size: 552 948 50 3653
Fragments arranged by size:
3653 948 552 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4464
Fragments arranged by size:
4464 683 56
BtsI GCAGTG_nn'
Cuts at: 2156 2719 3513 3533 2156
Size: 563 794 20 3826
Fragments arranged by size:
3826 794 563 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5168
DraI TTT'AAA
Cuts at: 2507 2507
Size: 5203
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4210 809
Size: 507 2894 1802
Fragments arranged by size:
2894 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2650 3539 5168
Size: 54 1425 50 275 18 820 889 1629
Cuts at: 5168 8
Size: 43
Fragments arranged by size:
1629 1425 889 820 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2650 1537
Size: 275 838 4090
Fragments arranged by size:
4090 838 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 3133 1353
Size: 828 952 3423
Fragments arranged by size:
3423 952 828
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3790 4164 4310 387
Size: 1064 138 2201 374 146 1280
Fragments arranged by size:
2201 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 3266 3863 4650 4918 1239
Size: 393 644 990 597 787 268 1524
Fragments arranged by size:
1524 990 787 644 597 393 268
EcoRI G'AATT_C
Cuts at: 2512 2890 2512
Size: 378 4825
FspI TGC'GCA
Cuts at: 3709 3709
Size: 5203
HaeII r_GCGC'y
Cuts at: 1678 2063 4350 1678
Size: 385 2287 2531
Fragments arranged by size:
2531 2287 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3844 3876 3918 3950 1373
Size: 32 135 32 2272 32 42 32 2626
Fragments arranged by size:
2626 2272 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2547 2793 669
Size: 988 281 609 246 3079
Fragments arranged by size:
3079 988 609 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5203
HpaI GTT'AAC
Cuts at: 2793 2793
Size: 5203
KasI G'GCGC_C
Cuts at: 2059 2059
Size: 5203
KpnI G_GTAC'C
Cuts at: 2528 2528
Size: 5203
MfeI C'AATT_G
Cuts at: 2802 2802
Size: 5203
MluI A'CGCG_T
Cuts at: 5079 5079
Size: 5203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4317 4501 1305
Size: 3012 184 2007
Fragments arranged by size:
3012 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3381
Fragments arranged by size:
3381 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3161 3520 3679 518
Size: 648 1995 359 159 2042
Fragments arranged by size:
2042 1995 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2619 3296 4444 4689 1606
Size: 1013 677 1148 245 2120
Fragments arranged by size:
2120 1148 1013 677 245
NarI GG'CG_CC
Cuts at: 2060 2060
Size: 5203
NcoI C'CATG_G
Cuts at: 513 1065 2063 513
Size: 552 998 3653
Fragments arranged by size:
3653 998 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5203
NheI G'CTAG_C
Cuts at: 1052 2323 1052
Size: 1271 3932
NotI GC'GGCC_GC
Cuts at: 2650 2650
Size: 5203
NspI r_CATG'y
Cuts at: 1132 1229 2561 4106 1132
Size: 97 1332 1545 2229
Fragments arranged by size:
2229 1545 1332 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5203
PciI A'CATG_T
Cuts at: 4102 4102
Size: 5203
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5203
PmeI GTTT'AAAC
Cuts at: 2507 2507
Size: 5203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 4785 4817 3272
Size: 32 1481 32 3658
Fragments arranged by size:
3658 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5203
PsiI TTA'TAA
Cuts at: 2773 2773
Size: 5203
PstI C_TGCA'G
Cuts at: 830 2555 830
Size: 1725 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5203
SalI G'TCGA_C
Cuts at: 1936 2545 1936
Size: 609 4594
SapI GCTCTTCn'nnn_
Cuts at: 2181 2181
Size: 5203
SbfI CC_TGCA'GG
Cuts at: 2555 2555
Size: 5203
ScaI AGT'ACT
Cuts at: 3451 5163 3451
Size: 1712 3491
SfcI C'TryA_G
Cuts at: 826 1045 2551 3686 4367 4558 826
Size: 219 1506 1135 681 191 1471
Fragments arranged by size:
1506 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2061 2061
Size: 5203
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5203
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5203
SmaI CCC'GGG
Cuts at: 1872 2530 1872
Size: 658 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3307 4208 4470 820
Size: 197 926 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2994 493
Size: 2501 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5203
SphI G_CATG'C
Cuts at: 1132 2561 1132
Size: 1429 3774
SspI AAT'ATT
Cuts at: 5 52 3127 5
Size: 47 3075 2081
Fragments arranged by size:
3075 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5203
StyI C'CwwG_G
Cuts at: 513 1065 2063 2166 2642 513
Size: 552 998 103 476 3074
Fragments arranged by size:
3074 998 552 476 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3230 3247 3400 3585 4030 325
Size: 1255 1650 17 153 185 445 1498
Fragments arranged by size:
1650 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3449 5161 96
Size: 274 80 33 51 1443 1472 1712 138
Fragments arranged by size:
1712 1472 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5163 5195 5163
Size: 32 5171
Tth111I GACn'n_nGTC
Cuts at: 1316 2208 1316
Size: 892 4311
XbaI T'CTAG_A
Cuts at: 2539 2539
Size: 5203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2245 2011
Size: 234 4969
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5203
XmaI C'CCGG_G
Cuts at: 1870 2528 1870
Size: 658 4545
XmnI GAAnn'nnTTC
Cuts at: 3332 3332
Size: 5203
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3011 276
Size: 53 83 186 936 1477 2468
Fragments arranged by size:
2468 1477 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI