Restriction map for
pFN21AE5121
(Circular) (Six-base) MAPSORT of: pFN21AE5121.seq Check: 4484 from: 1 to: 5074
pFN21AE5121 5074 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2884 278
Size: 53 83 186 936 1348 2468
Fragments arranged by size:
2468 1348 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2417 1937
Size: 480 4594
Acc65I G'GTAC_C
Cuts at: 2395 2395
Size: 5074
AclI AA'CG_TT
Cuts at: 1563 3201 3574 1563
Size: 1638 373 3063
Fragments arranged by size:
3063 1638 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2330 3137 4521 4789 1632
Size: 464 234 807 1384 268 1917
Fragments arranged by size:
1917 1384 807 464 268 234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4877
AflIII A'CryG_T
Cuts at: 1203 2267 3973 4950 1203
Size: 1064 1706 977 1327
Fragments arranged by size:
1706 1327 1064 977
AgeI A'CCGG_T
Cuts at: 3905 3905
Size: 5074
AhdI GACnn_n'nnGTC
Cuts at: 3803 3803
Size: 5074
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5074
AlwNI CAG_nnn'CTG
Cuts at: 2817 4389 2817
Size: 1572 3502
ApaLI G'TGCA_C
Cuts at: 3131 4287 3131
Size: 1156 3918
ApoI r'AATT_y
Cuts at: 1493 2216 2383 2609 2761 4870 1493
Size: 723 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 723 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 3628 160
Size: 872 2596 1606
Fragments arranged by size:
2596 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5074
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2399 1870
Size: 73 79 377 4545
Fragments arranged by size:
4545 377 79 73
BamHI G'GATC_C
Cuts at: 2404 2771 2404
Size: 367 4707
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2395 3850 618
Size: 325 217 627 608 1455 1842
Fragments arranged by size:
1842 1455 627 608 325 217
BanII G_rGCy'C
Cuts at: 1391 2393 1391
Size: 1002 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2270 2318 928
Size: 971 371 48 3684
Fragments arranged by size:
3684 971 371 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3265 3299 1903
Size: 34 1328 34 3678
Fragments arranged by size:
3678 1328 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2966 4176 2966
Size: 1210 3864
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5074
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3685 136
Size: 107 122 71 631 605 2013 1525
Fragments arranged by size:
2013 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2026 5069 2026
Size: 3043 2031
BlpI GC'TnA_GC
Cuts at: 2491 2491
Size: 5074
Bme1580I G_kGCm'C
Cuts at: 1790 3135 4291 1790
Size: 1345 1156 2573
Fragments arranged by size:
2573 1345 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2805 3763 442
Size: 416 53 83 399 24 1388 958 1753
Fragments arranged by size:
1753 1388 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3734 1239
Size: 2495 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3199 4064 4362 3199
Size: 865 298 3911
Fragments arranged by size:
3911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3737 882
Size: 617 2238 2219
Fragments arranged by size:
2238 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2270 2865 493
Size: 711 1066 595 2702
Fragments arranged by size:
2702 1066 711 595
BsaBI GATnn'nnATC
Cuts at: 5068 5068
Size: 5074
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2881 3263 3898 275
Size: 53 83 186 936 1348 382 635 1451
Fragments arranged by size:
1451 1348 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2154 3507 3905 4179 4326 4737 2154
Size: 1353 398 274 147 411 2491
Fragments arranged by size:
2491 1353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 1178
Size: 30 165 30 924 30 3895
Fragments arranged by size:
3895 924 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2315 1186
Size: 877 252 3945
Fragments arranged by size:
3945 877 252
BseYI C'CCAG_C
Cuts at: 2091 4277 4773 2091
Size: 2186 496 2392
Fragments arranged by size:
2392 2186 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4501
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2524 3285 4313 1540
Size: 275 186 523 761 1028 2301
Fragments arranged by size:
2301 1028 761 523 275 186
BsiHKAI G_wGCw'C
Cuts at: 3135 3220 4291 3135
Size: 85 1071 3918
Fragments arranged by size:
3918 1071 85
BsmI GAATG_Cn'
Cuts at: 2585 2678 2585
Size: 93 4981
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2393 3135 3220 4291 1391
Size: 399 603 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 85
BspEI T'CCGG_A
Cuts at: 2154 4737 2154
Size: 2583 2491
BspHI T'CATG_A
Cuts at: 2963 4693 2963
Size: 1730 3344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2429 844
Size: 1415 170 3489
Fragments arranged by size:
3489 1415 170
BsrBI CCG'CTC
Cuts at: 1394 1823 2338 2961 1394
Size: 429 515 623 3507
Fragments arranged by size:
3507 623 515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2163 3569 3743 65
Size: 1183 915 1406 174 1396
Fragments arranged by size:
1406 1396 1183 915 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2283 3718 3905 1700
Size: 110 473 1435 187 2869
Fragments arranged by size:
2869 1435 473 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5074
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5074
BssSI C'ACGA_G
Cuts at: 2297 3134 4146 2297
Size: 837 1012 3225
Fragments arranged by size:
3225 1012 837
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5074
BstBI TT'CG_AA
Cuts at: 3878 3878
Size: 5074
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5074
BstYI r'GATC_y
Cuts at: 1972 2026 2142 2404 2771 3156 3173 4625 4711
Size: 54 116 262 367 385 17 1452 86
Cuts at: 4711 4723 5069 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 262 116 86
54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2210 2210
Size: 5074
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3583
Fragments arranged by size:
3583 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4335
Fragments arranged by size:
4335 683 56
BtsI GCAGTG_nn'
Cuts at: 2590 3384 3404 2590
Size: 794 20 4260
Fragments arranged by size:
4260 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5074
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5039
DraI TTT'AAA
Cuts at: 2378 2378
Size: 5074
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4081 809
Size: 507 2765 1802
Fragments arranged by size:
2765 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2521 3410
Size: 54 1425 50 275 18 177 514 889
Cuts at: 3410 5039 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 514 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2521 1537
Size: 275 709 4090
Fragments arranged by size:
4090 709 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3004 1353
Size: 1651 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3661 4035 4181 387
Size: 1064 138 2072 374 146 1280
Fragments arranged by size:
2072 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2330 3137 3734 4521 4789 1239
Size: 393 464 234 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 464 393 268 234
EcoRI G'AATT_C
Cuts at: 2383 2761 2383
Size: 378 4696
FspI TGC'GCA
Cuts at: 3580 3580
Size: 5074
HaeII r_GCGC'y
Cuts at: 1678 4221 1678
Size: 2543 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2005 2037 3715 3747 3789
Size: 32 135 32 433 32 1678 32 42
Cuts at: 3789 3821 1373
Size: 32 2626
Fragments arranged by size:
2626 1678 433 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2418 2664 669
Size: 988 281 480 246 3079
Fragments arranged by size:
3079 988 480 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5074
HpaI GTT'AAC
Cuts at: 2664 2664
Size: 5074
KpnI G_GTAC'C
Cuts at: 2399 2399
Size: 5074
MfeI C'AATT_G
Cuts at: 2673 2673
Size: 5074
MluI A'CGCG_T
Cuts at: 4950 4950
Size: 5074
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4188 4372 1305
Size: 2883 184 2007
Fragments arranged by size:
2883 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3075
Fragments arranged by size:
3075 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3032 3391 3550 518
Size: 648 1866 359 159 2042
Fragments arranged by size:
2042 1866 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2095 2329 2350 2490 3167 4315 4560
Size: 459 30 234 21 140 677 1148 245
Cuts at: 4560 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 459 245 234 140 30
21
NaeI GCC'GGC
Cuts at: 2285 2285
Size: 5074
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3583
Fragments arranged by size:
3583 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5074
NgoMIV G'CCGG_C
Cuts at: 2283 2283
Size: 5074
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5074
NotI GC'GGCC_GC
Cuts at: 2521 2521
Size: 5074
NspI r_CATG'y
Cuts at: 1132 1229 2432 3977 1132
Size: 97 1203 1545 2229
Fragments arranged by size:
2229 1545 1203 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5074
PciI A'CATG_T
Cuts at: 3973 3973
Size: 5074
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5074
PmeI GTTT'AAAC
Cuts at: 2378 2378
Size: 5074
PmlI CAC'GTG
Cuts at: 2270 2270
Size: 5074
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 4656 4688 3143
Size: 32 1481 32 3529
Fragments arranged by size:
3529 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5074
PsiI TTA'TAA
Cuts at: 2644 2644
Size: 5074
PstI C_TGCA'G
Cuts at: 830 2426 830
Size: 1596 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5074
PvuII CAG'CTG
Cuts at: 2065 2095 2065
Size: 30 5044
SalI G'TCGA_C
Cuts at: 1936 2416 1936
Size: 480 4594
SbfI CC_TGCA'GG
Cuts at: 2426 2426
Size: 5074
ScaI AGT'ACT
Cuts at: 3322 5034 3322
Size: 1712 3362
SfcI C'TryA_G
Cuts at: 826 1045 2422 3557 4238 4429 826
Size: 219 1377 1135 681 191 1471
Fragments arranged by size:
1471 1377 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5074
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5074
SmaI CCC'GGG
Cuts at: 1872 2401 1872
Size: 529 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2022 3178 4079 4341 820
Size: 197 926 79 1156 901 262 1553
Fragments arranged by size:
1553 1156 926 901 262 197 79
SnaBI TAC'GTA
Cuts at: 493 2865 493
Size: 2372 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5074
SphI G_CATG'C
Cuts at: 1132 2432 1132
Size: 1300 3774
SspI AAT'ATT
Cuts at: 5 52 2998 5
Size: 47 2946 2081
Fragments arranged by size:
2946 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5074
StyI C'CwwG_G
Cuts at: 513 1065 2004 2513 513
Size: 552 939 509 3074
Fragments arranged by size:
3074 939 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3101 3118 3271 3456 3901 325
Size: 1255 1521 17 153 185 445 1498
Fragments arranged by size:
1521 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3320 5032
Size: 274 80 33 51 1443 258 1085 1712
Cuts at: 5032 96
Size: 138
Fragments arranged by size:
1712 1443 1085 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5034 5066 5034
Size: 32 5042
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5074
XbaI T'CTAG_A
Cuts at: 2410 2410
Size: 5074
XhoI C'TCGA_G
Cuts at: 1943 2022 1943
Size: 79 4995
XmaI C'CCGG_G
Cuts at: 1870 2399 1870
Size: 529 4545
XmnI GAAnn'nnTTC
Cuts at: 3203 3203
Size: 5074
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2882 276
Size: 53 83 186 936 1348 2468
Fragments arranged by size:
2468 1348 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI