Restriction map for
pFN21AE5120
(Circular) (Six-base) MAPSORT of: pFN21AE5120.seq Check: 7156 from: 1 to: 5014
pFN21AE5120 5014 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2824 278
Size: 53 83 186 936 1288 2468
Fragments arranged by size:
2468 1288 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2357 1937
Size: 420 4594
Acc65I G'GTAC_C
Cuts at: 2335 2335
Size: 5014
AclI AA'CG_TT
Cuts at: 1563 3141 3514 1563
Size: 1578 373 3063
Fragments arranged by size:
3063 1578 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2331 3077 4461 4729 1632
Size: 699 746 1384 268 1917
Fragments arranged by size:
1917 1384 746 699 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4817
AflIII A'CryG_T
Cuts at: 1203 3913 4890 1203
Size: 2710 977 1327
Fragments arranged by size:
2710 1327 977
AgeI A'CCGG_T
Cuts at: 3845 3845
Size: 5014
AhdI GACnn_n'nnGTC
Cuts at: 3743 3743
Size: 5014
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5014
AlwNI CAG_nnn'CTG
Cuts at: 2757 4329 2757
Size: 1572 3442
ApaLI G'TGCA_C
Cuts at: 3071 4227 3071
Size: 1156 3858
ApoI r'AATT_y
Cuts at: 1493 2323 2549 2701 4810 1493
Size: 830 226 152 2109 1697
Fragments arranged by size:
2109 1697 830 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3568 160
Size: 872 2536 1606
Fragments arranged by size:
2536 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5014
AvaI C'yCGr_G
Cuts at: 1870 1943 2339 1870
Size: 73 396 4545
Fragments arranged by size:
4545 396 73
BamHI G'GATC_C
Cuts at: 2344 2711 2344
Size: 367 4647
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2335 3790 618
Size: 325 217 627 548 1455 1842
Fragments arranged by size:
1842 1455 627 548 325 217
BanII G_rGCy'C
Cuts at: 1391 2333 1391
Size: 942 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3205 3239 1903
Size: 34 1268 34 3678
Fragments arranged by size:
3678 1268 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2906 4116 2906
Size: 1210 3804
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5014
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3625 136
Size: 107 122 71 631 605 1953 1525
Fragments arranged by size:
1953 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5009 5009
Size: 5014
BlpI GC'TnA_GC
Cuts at: 2431 2431
Size: 5014
Bme1580I G_kGCm'C
Cuts at: 1790 3075 4231 1790
Size: 1285 1156 2573
Fragments arranged by size:
2573 1285 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5014
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2745 3703 442
Size: 416 53 83 399 24 1328 958 1753
Fragments arranged by size:
1753 1328 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 3674 1239
Size: 918 1517 2579
Fragments arranged by size:
2579 1517 918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3139 4004 4302 3139
Size: 865 298 3851
Fragments arranged by size:
3851 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3677 882
Size: 617 2178 2219
Fragments arranged by size:
2219 2178 617
BsaAI yAC'GTr
Cuts at: 493 1204 2805 493
Size: 711 1601 2702
Fragments arranged by size:
2702 1601 711
BsaBI GATnn'nnATC
Cuts at: 5008 5008
Size: 5014
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2821 3203 3838 275
Size: 53 83 186 936 1288 382 635 1451
Fragments arranged by size:
1451 1288 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2046 3447 3845 4119 4266 4677 2046
Size: 1401 398 274 147 411 2383
Fragments arranged by size:
2383 1401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4789
Fragments arranged by size:
4789 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 1186
Size: 1126 3888
BseYI C'CCAG_C
Cuts at: 4217 4713 4217
Size: 496 4518
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2464 3225 4253 1540
Size: 275 186 463 761 1028 2301
Fragments arranged by size:
2301 1028 761 463 275 186
BsiHKAI G_wGCw'C
Cuts at: 3075 3160 4231 3075
Size: 85 1071 3858
Fragments arranged by size:
3858 1071 85
BsmI GAATG_Cn'
Cuts at: 2525 2618 2525
Size: 93 4921
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 3075 3160 4231 1391
Size: 399 543 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 399 85
BspEI T'CCGG_A
Cuts at: 2046 4677 2046
Size: 2631 2383
BspHI T'CATG_A
Cuts at: 2903 4633 2903
Size: 1730 3284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2369 844
Size: 1525 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2901 1394
Size: 429 1078 3507
Fragments arranged by size:
3507 1078 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3509 3683 65
Size: 1183 2261 174 1396
Fragments arranged by size:
2261 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3658 3845 1700
Size: 110 1848 187 2869
Fragments arranged by size:
2869 1848 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5014
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5014
BssSI C'ACGA_G
Cuts at: 3074 4086 3074
Size: 1012 4002
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2273 794
Size: 1479 3535
BstBI TT'CG_AA
Cuts at: 3818 3818
Size: 5014
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5014
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2095
Size: 5014
BstYI r'GATC_y
Cuts at: 1972 2344 2711 3096 3113 4565 4651 4663 5009
Size: 372 367 385 17 1452 86 12 346
Cuts at: 5009 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 372 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2292 2292
Size: 5014
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3523
Fragments arranged by size:
3523 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4275
Fragments arranged by size:
4275 683 56
BtsI GCAGTG_nn'
Cuts at: 2530 3324 3344 2530
Size: 794 20 4200
Fragments arranged by size:
4200 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4979
DraI TTT'AAA
Cuts at: 2148 2318 2148
Size: 170 4844
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4021 809
Size: 507 2705 1802
Fragments arranged by size:
2705 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2461 3350
Size: 54 1425 50 275 18 381 250 889
Cuts at: 3350 4979 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 381 275 250 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2461 1537
Size: 275 649 4090
Fragments arranged by size:
4090 649 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2944 1353
Size: 1591 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3601 3975 4121 387
Size: 1064 138 2012 374 146 1280
Fragments arranged by size:
2012 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2331 3077 3674 4461 4729 1239
Size: 393 525 174 746 597 787 268 1524
Fragments arranged by size:
1524 787 746 597 525 393 268 174
EcoRI G'AATT_C
Cuts at: 2323 2701 2323
Size: 378 4636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2288 2173
Size: 115 4899
FspI TGC'GCA
Cuts at: 3520 3520
Size: 5014
HaeII r_GCGC'y
Cuts at: 1678 4161 1678
Size: 2483 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3655 3687 3729 3761 1373
Size: 32 135 32 2083 32 42 32 2626
Fragments arranged by size:
2626 2083 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2358 2604 669
Size: 988 281 420 246 3079
Fragments arranged by size:
3079 988 420 281 246
HindIII A'AGCT_T
Cuts at: 748 2270 748
Size: 1522 3492
HpaI GTT'AAC
Cuts at: 2604 2604
Size: 5014
KpnI G_GTAC'C
Cuts at: 2339 2339
Size: 5014
MfeI C'AATT_G
Cuts at: 2613 2613
Size: 5014
MluI A'CGCG_T
Cuts at: 4890 4890
Size: 5014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4128 4312 1305
Size: 2823 184 2007
Fragments arranged by size:
2823 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3192
Fragments arranged by size:
3192 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2972 3331 3490 518
Size: 648 1806 359 159 2042
Fragments arranged by size:
2042 1806 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2430 3107 4255 4500 1606
Size: 824 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3523
Fragments arranged by size:
3523 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5014
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5014
NotI GC'GGCC_GC
Cuts at: 2461 2461
Size: 5014
NspI r_CATG'y
Cuts at: 1132 1229 2372 3917 1132
Size: 97 1143 1545 2229
Fragments arranged by size:
2229 1545 1143 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5014
PciI A'CATG_T
Cuts at: 3913 3913
Size: 5014
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5014
PmeI GTTT'AAAC
Cuts at: 2318 2318
Size: 5014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 4596 4628 3083
Size: 32 1481 32 3469
Fragments arranged by size:
3469 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5014
PsiI TTA'TAA
Cuts at: 2584 2584
Size: 5014
PstI C_TGCA'G
Cuts at: 830 2366 830
Size: 1536 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5014
SalI G'TCGA_C
Cuts at: 1936 2356 1936
Size: 420 4594
SbfI CC_TGCA'GG
Cuts at: 2366 2366
Size: 5014
ScaI AGT'ACT
Cuts at: 3262 4974 3262
Size: 1712 3302
SfcI C'TryA_G
Cuts at: 826 1045 2362 3497 4178 4369 826
Size: 219 1317 1135 681 191 1471
Fragments arranged by size:
1471 1317 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5014
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5014
SmaI CCC'GGG
Cuts at: 1872 2341 1872
Size: 469 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3118 4019 4281 820
Size: 197 926 1175 901 262 1553
Fragments arranged by size:
1553 1175 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2805 493
Size: 2312 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5014
SphI G_CATG'C
Cuts at: 1132 2372 1132
Size: 1240 3774
SspI AAT'ATT
Cuts at: 5 52 2938 5
Size: 47 2886 2081
Fragments arranged by size:
2886 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5014
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2453 513
Size: 552 939 84 365 3074
Fragments arranged by size:
3074 939 552 365 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3041 3058 3211 3396 3841 325
Size: 1255 1461 17 153 185 445 1498
Fragments arranged by size:
1498 1461 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3260 4972 96
Size: 274 80 33 51 1443 1283 1712 138
Fragments arranged by size:
1712 1443 1283 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4974 5006 4974
Size: 32 4982
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5014
XbaI T'CTAG_A
Cuts at: 2350 2350
Size: 5014
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5014
XmaI C'CCGG_G
Cuts at: 1870 2339 1870
Size: 469 4545
XmnI GAAnn'nnTTC
Cuts at: 3143 3143
Size: 5014
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2822 276
Size: 53 83 186 936 1288 2468
Fragments arranged by size:
2468 1288 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI