Restriction map for
pFN21AE5109
(Circular) (Six-base) MAPSORT of: pFN21AE5109.seq Check: 9613 from: 1 to: 5182
pFN21AE5109 5182 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2992 278
Size: 53 83 186 936 1456 2468
Fragments arranged by size:
2468 1456 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2525 1937
Size: 588 4594
Acc65I G'GTAC_C
Cuts at: 2503 2503
Size: 5182
AclI AA'CG_TT
Cuts at: 1563 3309 3682 1563
Size: 1746 373 3063
Fragments arranged by size:
3063 1746 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 3245 4629 4897 1632
Size: 461 1152 1384 268 1917
Fragments arranged by size:
1917 1384 1152 461 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4985
AflIII A'CryG_T
Cuts at: 1203 4081 5058 1203
Size: 2878 977 1327
Fragments arranged by size:
2878 1327 977
AgeI A'CCGG_T
Cuts at: 4013 4013
Size: 5182
AhdI GACnn_n'nnGTC
Cuts at: 3911 3911
Size: 5182
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2388 1184
Size: 1204 3978
AlwNI CAG_nnn'CTG
Cuts at: 2925 4497 2925
Size: 1572 3610
ApaLI G'TGCA_C
Cuts at: 3239 4395 3239
Size: 1156 4026
ApoI r'AATT_y
Cuts at: 1493 2491 2717 2869 4978 1493
Size: 998 226 152 2109 1697
Fragments arranged by size:
2109 1697 998 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3736 160
Size: 872 2704 1606
Fragments arranged by size:
2704 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5182
AvaI C'yCGr_G
Cuts at: 1870 1943 2507 1870
Size: 73 564 4545
Fragments arranged by size:
4545 564 73
AvrII C'CTAG_G
Cuts at: 2044 2044
Size: 5182
BamHI G'GATC_C
Cuts at: 2512 2879 2512
Size: 367 4815
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2503 3958 618
Size: 325 217 627 252 464 1455 1842
Fragments arranged by size:
1842 1455 627 464 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2059 2501 1391
Size: 668 442 4072
Fragments arranged by size:
4072 668 442
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3373 3407 1903
Size: 34 1436 34 3678
Fragments arranged by size:
3678 1436 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3074 4284 3074
Size: 1210 3972
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3793 136
Size: 107 122 71 631 605 2121 1525
Fragments arranged by size:
2121 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5177 5177
Size: 5182
BlpI GC'TnA_GC
Cuts at: 2599 2599
Size: 5182
Bme1580I G_kGCm'C
Cuts at: 1790 2044 3243 4399 1790
Size: 254 1199 1156 2573
Fragments arranged by size:
2573 1199 1156 254
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5182
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 2913 3871
Size: 416 53 83 399 24 909 587 958
Cuts at: 3871 442
Size: 1753
Fragments arranged by size:
1753 958 909 587 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2058 3842 1239
Size: 799 20 1784 2579
Fragments arranged by size:
2579 1784 799 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3307 4172 4470 3307
Size: 865 298 4019
Fragments arranged by size:
4019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3845 882
Size: 617 2346 2219
Fragments arranged by size:
2346 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2973 493
Size: 711 1769 2702
Fragments arranged by size:
2702 1769 711
BsaBI GATnn'nnATC
Cuts at: 5176 5176
Size: 5182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2989 3371 4006 275
Size: 53 83 186 936 1456 382 635 1451
Fragments arranged by size:
1456 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3615 4013 4287 4434 4845 3615
Size: 398 274 147 411 3952
Fragments arranged by size:
3952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2011 2041 1178
Size: 30 165 30 608 30 4319
Fragments arranged by size:
4319 608 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5182
BseYI C'CCAG_C
Cuts at: 2414 4385 4881 2414
Size: 1971 496 2715
Fragments arranged by size:
2715 1971 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 1163
Size: 573 426 4183
Fragments arranged by size:
4183 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2632 3393 4421 1540
Size: 275 186 631 761 1028 2301
Fragments arranged by size:
2301 1028 761 631 275 186
BsiHKAI G_wGCw'C
Cuts at: 2059 3243 3328 4399 2059
Size: 1184 85 1071 2842
Fragments arranged by size:
2842 1184 1071 85
BsmI GAATG_Cn'
Cuts at: 2693 2786 2693
Size: 93 5089
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2059 2501 3243 3328 4399 1391
Size: 399 254 15 442 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 254 85 15
BspEI T'CCGG_A
Cuts at: 4845 4845
Size: 5182
BspHI T'CATG_A
Cuts at: 3071 4801 3071
Size: 1730 3452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 2537 844
Size: 1251 442 3489
Fragments arranged by size:
3489 1251 442
BsrBI CCG'CTC
Cuts at: 1394 1823 3069 1394
Size: 429 1246 3507
Fragments arranged by size:
3507 1246 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3677 3851 65
Size: 1183 2429 174 1396
Fragments arranged by size:
2429 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3826 4013 1700
Size: 110 2016 187 2869
Fragments arranged by size:
2869 2016 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5182
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5182
BssSI C'ACGA_G
Cuts at: 3242 4254 3242
Size: 1012 4170
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5182
BstBI TT'CG_AA
Cuts at: 2068 3986 2068
Size: 1918 3264
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5182
BstXI CCAn_nnnn'nTGG
Cuts at: 2174 2174
Size: 5182
BstYI r'GATC_y
Cuts at: 1972 2512 2879 3264 3281 4733 4819 4831 5177
Size: 540 367 385 17 1452 86 12 346
Cuts at: 5177 1972
Size: 1977
Fragments arranged by size:
1977 1452 540 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3691
Fragments arranged by size:
3691 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2288 487
Size: 683 56 1062 3381
Fragments arranged by size:
3381 1062 683 56
BtsI GCAGTG_nn'
Cuts at: 2698 3492 3512 2698
Size: 794 20 4368
Fragments arranged by size:
4368 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5182
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5147
DraI TTT'AAA
Cuts at: 2486 2486
Size: 5182
DraIII CAC_nnn'GTG
Cuts at: 2443 2443
Size: 5182
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4189 809
Size: 507 2873 1802
Fragments arranged by size:
2873 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2629 3518 5147
Size: 54 1425 50 275 18 799 889 1629
Cuts at: 5147 8
Size: 43
Fragments arranged by size:
1629 1425 889 799 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2629 1537
Size: 275 817 4090
Fragments arranged by size:
4090 817 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 3112 1353
Size: 957 802 3423
Fragments arranged by size:
3423 957 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3769 4143 4289 387
Size: 1064 138 2180 374 146 1280
Fragments arranged by size:
2180 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2057 2057
Size: 5182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2058 2093 3245 3842 4629 4897
Size: 393 406 20 35 1152 597 787 268
Cuts at: 4897 1239
Size: 1524
Fragments arranged by size:
1524 1152 787 597 406 393 268 35
20
EcoO109I rG'GnC_Cy
Cuts at: 2320 2320
Size: 5182
EcoRI G'AATT_C
Cuts at: 2491 2869 2491
Size: 378 4804
FspI TGC'GCA
Cuts at: 3688 3688
Size: 5182
HaeII r_GCGC'y
Cuts at: 1678 4329 1678
Size: 2651 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3823 3855 3897 3929 1373
Size: 32 135 32 2251 32 42 32 2626
Fragments arranged by size:
2626 2251 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2526 2772 669
Size: 988 281 588 246 3079
Fragments arranged by size:
3079 988 588 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5182
HpaI GTT'AAC
Cuts at: 2772 2772
Size: 5182
KpnI G_GTAC'C
Cuts at: 2507 2507
Size: 5182
MfeI C'AATT_G
Cuts at: 2781 2781
Size: 5182
MluI A'CGCG_T
Cuts at: 5058 5058
Size: 5182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4296 4480 1305
Size: 2991 184 2007
Fragments arranged by size:
2991 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3360
Fragments arranged by size:
3360 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 3140 3499 3658 518
Size: 648 1189 785 359 159 2042
Fragments arranged by size:
2042 1189 785 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2598 3275 4423 4668 1606
Size: 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3691
Fragments arranged by size:
3691 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5182
NotI GC'GGCC_GC
Cuts at: 2629 2629
Size: 5182
NspI r_CATG'y
Cuts at: 1132 1229 2540 4085 1132
Size: 97 1311 1545 2229
Fragments arranged by size:
2229 1545 1311 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5182
PciI A'CATG_T
Cuts at: 4081 4081
Size: 5182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2472 1108
Size: 1364 3818
PmeI GTTT'AAAC
Cuts at: 2486 2486
Size: 5182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 4764 4796 3251
Size: 32 1481 32 3637
Fragments arranged by size:
3637 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5182
PsiI TTA'TAA
Cuts at: 2752 2752
Size: 5182
PstI C_TGCA'G
Cuts at: 830 2296 2534 830
Size: 1466 238 3478
Fragments arranged by size:
3478 1466 238
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5182
SacI G_AGCT'C
Cuts at: 2059 2059
Size: 5182
SalI G'TCGA_C
Cuts at: 1936 2524 1936
Size: 588 4594
SbfI CC_TGCA'GG
Cuts at: 2534 2534
Size: 5182
ScaI AGT'ACT
Cuts at: 3430 5142 3430
Size: 1712 3470
SfcI C'TryA_G
Cuts at: 826 1045 2292 2530 3665 4346 4537 826
Size: 219 1247 238 1135 681 191 1471
Fragments arranged by size:
1471 1247 1135 681 238 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5182
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5182
SmaI CCC'GGG
Cuts at: 1872 2509 1872
Size: 637 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3286 4187 4449 820
Size: 197 926 1343 901 262 1553
Fragments arranged by size:
1553 1343 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2973 493
Size: 2480 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5182
SphI G_CATG'C
Cuts at: 1132 2540 1132
Size: 1408 3774
SspI AAT'ATT
Cuts at: 5 52 3106 5
Size: 47 3054 2081
Fragments arranged by size:
3054 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5182
StyI C'CwwG_G
Cuts at: 513 1065 2004 2044 2181 2323 2376 2621 513
Size: 552 939 40 137 142 53 245 3074
Fragments arranged by size:
3074 939 552 245 142 137 53 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2130 3209 3226 3379 3564 4009 325
Size: 1255 550 1079 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1079 550 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3428 5140 96
Size: 274 80 33 51 1443 1451 1712 138
Fragments arranged by size:
1712 1451 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 5142 5174 2011
Size: 32 3099 32 2019
Fragments arranged by size:
3099 2019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5182
XbaI T'CTAG_A
Cuts at: 2518 2518
Size: 5182
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5182
XmaI C'CCGG_G
Cuts at: 1870 2507 1870
Size: 637 4545
XmnI GAAnn'nnTTC
Cuts at: 3311 3311
Size: 5182
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2990 276
Size: 53 83 186 936 1456 2468
Fragments arranged by size:
2468 1456 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI