Restriction map for
pFN21AE5104
(Circular) (Six-base) MAPSORT of: pFN21AE5104.seq Check: 6490 from: 1 to: 5056
pFN21AE5104 5056 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2866 278
Size: 53 83 186 936 1330 2468
Fragments arranged by size:
2468 1330 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2399 1937
Size: 462 4594
Acc65I G'GTAC_C
Cuts at: 2377 2377
Size: 5056
AclI AA'CG_TT
Cuts at: 1563 3183 3556 1563
Size: 1620 373 3063
Fragments arranged by size:
3063 1620 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3119 4503 4771 1632
Size: 1487 1384 268 1917
Fragments arranged by size:
1917 1487 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4859
AflIII A'CryG_T
Cuts at: 1203 2221 3955 4932 1203
Size: 1018 1734 977 1327
Fragments arranged by size:
1734 1327 1018 977
AgeI A'CCGG_T
Cuts at: 3887 3887
Size: 5056
AhdI GACnn_n'nnGTC
Cuts at: 3785 3785
Size: 5056
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5056
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5056
AlwNI CAG_nnn'CTG
Cuts at: 2799 4371 2799
Size: 1572 3484
ApaLI G'TGCA_C
Cuts at: 3113 4269 3113
Size: 1156 3900
ApoI r'AATT_y
Cuts at: 1493 2365 2591 2743 4852 1493
Size: 872 226 152 2109 1697
Fragments arranged by size:
2109 1697 872 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3610 160
Size: 872 2578 1606
Fragments arranged by size:
2578 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5056
AvaI C'yCGr_G
Cuts at: 1870 1943 2381 1870
Size: 73 438 4545
Fragments arranged by size:
4545 438 73
BamHI G'GATC_C
Cuts at: 2386 2753 2386
Size: 367 4689
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2377 3832 618
Size: 325 217 627 590 1455 1842
Fragments arranged by size:
1842 1455 627 590 325 217
BanII G_rGCy'C
Cuts at: 1391 2375 1391
Size: 984 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3247 3281 1903
Size: 34 1310 34 3678
Fragments arranged by size:
3678 1310 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2948 4158 2948
Size: 1210 3846
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5056
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3667 136
Size: 107 122 71 631 605 1995 1525
Fragments arranged by size:
1995 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5051 5051
Size: 5056
BlpI GC'TnA_GC
Cuts at: 2473 2473
Size: 5056
Bme1580I G_kGCm'C
Cuts at: 1790 2153 3117 4273 1790
Size: 363 964 1156 2573
Fragments arranged by size:
2573 1156 964 363
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5056
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2787 3745 442
Size: 416 53 83 399 24 1370 958 1753
Fragments arranged by size:
1753 1370 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3716 1239
Size: 2477 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3181 4046 4344 3181
Size: 865 298 3893
Fragments arranged by size:
3893 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3719 882
Size: 617 2220 2219
Fragments arranged by size:
2220 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2847 493
Size: 711 1643 2702
Fragments arranged by size:
2702 1643 711
BsaBI GATnn'nnATC
Cuts at: 5050 5050
Size: 5056
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2863 3245 3880 275
Size: 53 83 186 936 1330 382 635 1451
Fragments arranged by size:
1451 1330 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3489 3887 4161 4308 4719 3489
Size: 398 274 147 411 3826
Fragments arranged by size:
3826 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4831
Fragments arranged by size:
4831 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 1186
Size: 1126 3930
BseYI C'CCAG_C
Cuts at: 4259 4755 4259
Size: 496 4560
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2506 3267 4295 1540
Size: 275 186 505 761 1028 2301
Fragments arranged by size:
2301 1028 761 505 275 186
BsiHKAI G_wGCw'C
Cuts at: 3117 3202 4273 3117
Size: 85 1071 3900
Fragments arranged by size:
3900 1071 85
BsmI GAATG_Cn'
Cuts at: 2567 2660 2567
Size: 93 4963
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2375 3117 3202 4273 1391
Size: 399 363 222 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 222 85
BspEI T'CCGG_A
Cuts at: 4719 4719
Size: 5056
BspHI T'CATG_A
Cuts at: 2945 4675 2945
Size: 1730 3326
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2411 844
Size: 1567 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2943 1394
Size: 429 1120 3507
Fragments arranged by size:
3507 1120 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3551 3725 65
Size: 1183 2303 174 1396
Fragments arranged by size:
2303 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3700 3887 1700
Size: 110 1890 187 2869
Fragments arranged by size:
2869 1890 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5056
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5056
BssSI C'ACGA_G
Cuts at: 3116 4128 3116
Size: 1012 4044
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5056
BstBI TT'CG_AA
Cuts at: 3860 3860
Size: 5056
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5056
BstYI r'GATC_y
Cuts at: 1972 2386 2753 3138 3155 4607 4693 4705 5051
Size: 414 367 385 17 1452 86 12 346
Cuts at: 5051 1972
Size: 1977
Fragments arranged by size:
1977 1452 414 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3565
Fragments arranged by size:
3565 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4317
Fragments arranged by size:
4317 683 56
BtsI GCAGTG_nn'
Cuts at: 2572 3366 3386 2572
Size: 794 20 4242
Fragments arranged by size:
4242 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5056
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5021
DraI TTT'AAA
Cuts at: 2360 2360
Size: 5056
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4063 809
Size: 507 2747 1802
Fragments arranged by size:
2747 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2503 3392 5021
Size: 54 1425 50 275 18 673 889 1629
Cuts at: 5021 8
Size: 43
Fragments arranged by size:
1629 1425 889 673 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2503 1537
Size: 275 691 4090
Fragments arranged by size:
4090 691 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2986 1353
Size: 1633 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3643 4017 4163 387
Size: 1064 138 2054 374 146 1280
Fragments arranged by size:
2054 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3119 3716 4503 4771 1239
Size: 393 1487 597 787 268 1524
Fragments arranged by size:
1524 1487 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2294
Size: 5056
EcoO109I rG'GnC_Cy
Cuts at: 2232 2232
Size: 5056
EcoRI G'AATT_C
Cuts at: 2365 2743 2365
Size: 378 4678
FspI TGC'GCA
Cuts at: 3562 3562
Size: 5056
HaeII r_GCGC'y
Cuts at: 1678 4203 1678
Size: 2525 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3697 3729 3771 3803 1373
Size: 32 135 32 2125 32 42 32 2626
Fragments arranged by size:
2626 2125 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2400 2646 669
Size: 988 281 462 246 3079
Fragments arranged by size:
3079 988 462 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5056
HpaI GTT'AAC
Cuts at: 2646 2646
Size: 5056
KpnI G_GTAC'C
Cuts at: 2381 2381
Size: 5056
MfeI C'AATT_G
Cuts at: 2655 2655
Size: 5056
MluI A'CGCG_T
Cuts at: 4932 4932
Size: 5056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4170 4354 1305
Size: 2865 184 2007
Fragments arranged by size:
2865 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3234
Fragments arranged by size:
3234 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3014 3373 3532 518
Size: 648 1848 359 159 2042
Fragments arranged by size:
2042 1848 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2472 3149 4297 4542 1606
Size: 866 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3565
Fragments arranged by size:
3565 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5056
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5056
NotI GC'GGCC_GC
Cuts at: 2503 2503
Size: 5056
NspI r_CATG'y
Cuts at: 1132 1229 2142 2225 2414 3959 1132
Size: 97 913 83 189 1545 2229
Fragments arranged by size:
2229 1545 913 189 97 83
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5056
PciI A'CATG_T
Cuts at: 2221 3955 2221
Size: 1734 3322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5056
PmeI GTTT'AAAC
Cuts at: 2360 2360
Size: 5056
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 4638 4670 3125
Size: 32 1481 32 3511
Fragments arranged by size:
3511 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5056
PsiI TTA'TAA
Cuts at: 2626 2626
Size: 5056
PstI C_TGCA'G
Cuts at: 830 2408 830
Size: 1578 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5056
SalI G'TCGA_C
Cuts at: 1936 2398 1936
Size: 462 4594
SbfI CC_TGCA'GG
Cuts at: 2408 2408
Size: 5056
ScaI AGT'ACT
Cuts at: 3304 5016 3304
Size: 1712 3344
SfcI C'TryA_G
Cuts at: 826 1045 2404 3539 4220 4411 826
Size: 219 1359 1135 681 191 1471
Fragments arranged by size:
1471 1359 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5056
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5056
SmaI CCC'GGG
Cuts at: 1872 2383 1872
Size: 511 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3160 4061 4323 820
Size: 197 926 1217 901 262 1553
Fragments arranged by size:
1553 1217 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2847 493
Size: 2354 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5056
SphI G_CATG'C
Cuts at: 1132 2414 1132
Size: 1282 3774
SspI AAT'ATT
Cuts at: 5 52 2980 5
Size: 47 2928 2081
Fragments arranged by size:
2928 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5056
StyI C'CwwG_G
Cuts at: 513 1065 2004 2495 513
Size: 552 939 491 3074
Fragments arranged by size:
3074 939 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3083 3100 3253 3438 3883 325
Size: 1255 1503 17 153 185 445 1498
Fragments arranged by size:
1503 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3302 5014 96
Size: 274 80 33 51 1443 1325 1712 138
Fragments arranged by size:
1712 1443 1325 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2266 2298 5016 5048 2266
Size: 32 2718 32 2274
Fragments arranged by size:
2718 2274 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5056
XbaI T'CTAG_A
Cuts at: 2392 2392
Size: 5056
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5056
XmaI C'CCGG_G
Cuts at: 1870 2381 1870
Size: 511 4545
XmnI GAAnn'nnTTC
Cuts at: 3185 3185
Size: 5056
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2864 276
Size: 53 83 186 936 1330 2468
Fragments arranged by size:
2468 1330 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI