Restriction map for
pFN21AE5086
(Circular) (Six-base) MAPSORT of: pFN21AE5086.seq Check: 128 from: 1 to: 4942
pFN21AE5086 4942 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2752 278
Size: 53 83 186 936 1216 2468
Fragments arranged by size:
2468 1216 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2285 1937
Size: 348 4594
Acc65I G'GTAC_C
Cuts at: 2263 2263
Size: 4942
AclI AA'CG_TT
Cuts at: 1563 3069 3442 1563
Size: 1506 373 3063
Fragments arranged by size:
3063 1506 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 2228 3005 4389 4657 1632
Size: 433 163 777 1384 268 1917
Fragments arranged by size:
1917 1384 777 433 268 163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4745
AflIII A'CryG_T
Cuts at: 1203 3841 4818 1203
Size: 2638 977 1327
Fragments arranged by size:
2638 1327 977
AgeI A'CCGG_T
Cuts at: 3773 3773
Size: 4942
AhdI GACnn_n'nnGTC
Cuts at: 3671 3671
Size: 4942
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4942
AlwNI CAG_nnn'CTG
Cuts at: 2685 4257 2685
Size: 1572 3370
ApaLI G'TGCA_C
Cuts at: 2999 4155 2999
Size: 1156 3786
ApoI r'AATT_y
Cuts at: 1493 2251 2477 2629 4738 1493
Size: 758 226 152 2109 1697
Fragments arranged by size:
2109 1697 758 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3496 160
Size: 872 2464 1606
Fragments arranged by size:
2464 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4942
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2267 1870
Size: 73 117 207 4545
Fragments arranged by size:
4545 207 117 73
BamHI G'GATC_C
Cuts at: 2272 2639 2272
Size: 367 4575
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2263 3718 618
Size: 325 217 627 476 1455 1842
Fragments arranged by size:
1842 1455 627 476 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2261 1391
Size: 678 192 4072
Fragments arranged by size:
4072 678 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 928
Size: 971 138 3833
Fragments arranged by size:
3833 971 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3133 3167 1903
Size: 34 1196 34 3678
Fragments arranged by size:
3678 1196 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2834 4044 2834
Size: 1210 3732
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4942
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2157 3553
Size: 107 122 71 631 605 377 108 1396
Cuts at: 3553 136
Size: 1525
Fragments arranged by size:
1525 1396 631 605 377 122 108 107
71
BglII A'GATC_T
Cuts at: 4937 4937
Size: 4942
BlpI GC'TnA_GC
Cuts at: 2359 2359
Size: 4942
Bme1580I G_kGCm'C
Cuts at: 1790 3003 4159 1790
Size: 1213 1156 2573
Fragments arranged by size:
2573 1213 1156
BmgBI CAC'GTC
Cuts at: 1318 2203 1318
Size: 885 4057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2673 3631 442
Size: 416 53 83 399 24 1256 958 1753
Fragments arranged by size:
1753 1256 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3602 1239
Size: 2363 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3067 3932 4230 3067
Size: 865 298 3779
Fragments arranged by size:
3779 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3605 882
Size: 617 2106 2219
Fragments arranged by size:
2219 2106 617
BsaAI yAC'GTr
Cuts at: 493 1204 2733 493
Size: 711 1529 2702
Fragments arranged by size:
2702 1529 711
BsaBI GATnn'nnATC
Cuts at: 4936 4936
Size: 4942
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2749 3131 3766 275
Size: 53 83 186 936 1216 382 635 1451
Fragments arranged by size:
1451 1216 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2190 2234 3375 3773 4047 4194 4605 2190
Size: 44 1141 398 274 147 411 2527
Fragments arranged by size:
2527 1141 411 398 274 147 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 1178
Size: 30 165 30 687 30 4000
Fragments arranged by size:
4000 687 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4942
BseYI C'CCAG_C
Cuts at: 2146 4145 4641 2146
Size: 1999 496 2447
Fragments arranged by size:
2447 1999 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2161 2392 3153 4181 1540
Size: 275 186 160 231 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 231 186 160
BsiHKAI G_wGCw'C
Cuts at: 3003 3088 4159 3003
Size: 85 1071 3786
Fragments arranged by size:
3786 1071 85
BsmI GAATG_Cn'
Cuts at: 2453 2546 2453
Size: 93 4849
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2261 3003 3088 4159 1391
Size: 399 279 192 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 279 192 85
BspEI T'CCGG_A
Cuts at: 2234 4605 2234
Size: 2371 2571
BspHI T'CATG_A
Cuts at: 2831 4561 2831
Size: 1730 3212
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2297 844
Size: 1453 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2829 1394
Size: 429 1006 3507
Fragments arranged by size:
3507 1006 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3437 3611 65
Size: 1183 2189 174 1396
Fragments arranged by size:
2189 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3586 3773 1700
Size: 110 1776 187 2869
Fragments arranged by size:
2869 1776 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4942
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4942
BssSI C'ACGA_G
Cuts at: 3002 4014 3002
Size: 1012 3930
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4942
BstBI TT'CG_AA
Cuts at: 3746 3746
Size: 4942
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4942
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 4942
BstYI r'GATC_y
Cuts at: 1972 2013 2272 2639 3024 3041 4493 4579 4591
Size: 41 259 367 385 17 1452 86 12
Cuts at: 4591 4937 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 259 86 41
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4390
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4203
Fragments arranged by size:
4203 683 56
BtsI GCAGTG_nn'
Cuts at: 2458 3252 3272 2458
Size: 794 20 4128
Fragments arranged by size:
4128 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4907
DraI TTT'AAA
Cuts at: 2246 2246
Size: 4942
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3949 809
Size: 507 2633 1802
Fragments arranged by size:
2633 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 2389 3278
Size: 54 1425 50 275 18 328 231 889
Cuts at: 3278 4907 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 328 275 231 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2158 2389 1537
Size: 275 346 231 4090
Fragments arranged by size:
4090 346 275 231
EarI CTCTTCn'nnn_
Cuts at: 1353 2872 1353
Size: 1519 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3529 3903 4049 387
Size: 1064 138 1940 374 146 1280
Fragments arranged by size:
1940 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2228 3005 3602 4389 4657 1239
Size: 393 433 163 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 597 433 393 268 163
EcoRI G'AATT_C
Cuts at: 2251 2629 2251
Size: 378 4564
FspI TGC'GCA
Cuts at: 3448 3448
Size: 4942
HaeII r_GCGC'y
Cuts at: 1678 4089 1678
Size: 2411 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2163 2195 3583
Size: 32 135 32 518 32 41 32 1388
Cuts at: 3583 3615 3657 3689 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1388 518 135 42 41 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2286 2532 669
Size: 988 281 348 246 3079
Fragments arranged by size:
3079 988 348 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4942
HpaI GTT'AAC
Cuts at: 2532 2532
Size: 4942
KpnI G_GTAC'C
Cuts at: 2267 2267
Size: 4942
MfeI C'AATT_G
Cuts at: 2541 2541
Size: 4942
MluI A'CGCG_T
Cuts at: 4818 4818
Size: 4942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 4056 4240 1305
Size: 789 1962 184 2007
Fragments arranged by size:
2007 1962 789 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3120
Fragments arranged by size:
3120 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2900 3259 3418 518
Size: 648 1734 359 159 2042
Fragments arranged by size:
2042 1734 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2358 3035 4183 4428 1606
Size: 479 273 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 479 273 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4390
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4942
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4942
NotI GC'GGCC_GC
Cuts at: 2389 2389
Size: 4942
NspI r_CATG'y
Cuts at: 1132 1229 2300 3845 1132
Size: 97 1071 1545 2229
Fragments arranged by size:
2229 1545 1071 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4942
PciI A'CATG_T
Cuts at: 3841 3841
Size: 4942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4942
PmeI GTTT'AAAC
Cuts at: 2246 2246
Size: 4942
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 4524 4556 3011
Size: 32 1481 32 3397
Fragments arranged by size:
3397 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4942
PsiI TTA'TAA
Cuts at: 2512 2512
Size: 4942
PstI C_TGCA'G
Cuts at: 830 2097 2294 830
Size: 1267 197 3478
Fragments arranged by size:
3478 1267 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4942
PvuII CAG'CTG
Cuts at: 2085 2085
Size: 4942
SalI G'TCGA_C
Cuts at: 1936 2284 1936
Size: 348 4594
SbfI CC_TGCA'GG
Cuts at: 2097 2294 2097
Size: 197 4745
ScaI AGT'ACT
Cuts at: 3190 4902 3190
Size: 1712 3230
SfcI C'TryA_G
Cuts at: 826 1045 2023 2093 2290 3425 4106 4297 826
Size: 219 978 70 197 1135 681 191 1471
Fragments arranged by size:
1471 1135 978 681 219 197 191 70
SfiI GGCCn_nnn'nGGCC
Cuts at: 2157 2157
Size: 4942
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4942
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4942
SmaI CCC'GGG
Cuts at: 1872 2269 1872
Size: 397 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3046 3947 4209 820
Size: 197 926 1103 901 262 1553
Fragments arranged by size:
1553 1103 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2733 493
Size: 2240 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4942
SphI G_CATG'C
Cuts at: 1132 2300 1132
Size: 1168 3774
SspI AAT'ATT
Cuts at: 5 52 2866 5
Size: 47 2814 2081
Fragments arranged by size:
2814 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4942
StyI C'CwwG_G
Cuts at: 513 1065 2381 513
Size: 552 1316 3074
Fragments arranged by size:
3074 1316 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2969 2986 3139 3324 3769 325
Size: 1255 1389 17 153 185 445 1498
Fragments arranged by size:
1498 1389 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3188 4900 96
Size: 274 80 33 51 1443 1211 1712 138
Fragments arranged by size:
1712 1443 1211 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4902 4934 4902
Size: 32 4910
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4942
XbaI T'CTAG_A
Cuts at: 2278 2278
Size: 4942
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4942
XmaI C'CCGG_G
Cuts at: 1870 2267 1870
Size: 397 4545
XmnI GAAnn'nnTTC
Cuts at: 3071 3071
Size: 4942
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2750 276
Size: 53 83 186 936 1216 2468
Fragments arranged by size:
2468 1216 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfoI SrfI SwaI XcmI