Restriction map for
pFN21AE5080
(Circular) (Six-base) MAPSORT of: pFN21AE5080.seq Check: 8944 from: 1 to: 5668
pFN21AE5080 5668 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 5668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2279 3731 5115 5383 1632
Size: 419 228 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 419 268 228
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2551 3411 4983 2551
Size: 860 1572 3236
Fragments arranged by size:
3236 1572 860
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2248 2573 2977 3203 3355 5464 1493
Size: 755 325 404 226 152 2109 1697
Fragments arranged by size:
2109 1697 755 404 325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2580 4222 160
Size: 872 1548 1642 1606
Fragments arranged by size:
1642 1606 1548 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BamHI G'GATC_C
Cuts at: 2407 2998 3365 2407
Size: 591 367 4710
Fragments arranged by size:
4710 591 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2989 4444 618
Size: 325 217 627 1202 1455 1842
Fragments arranged by size:
1842 1455 1202 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2469 2987 1391
Size: 1078 518 4072
Fragments arranged by size:
4072 1078 518
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2701 928
Size: 971 802 3895
Fragments arranged by size:
3895 971 802
BbvCI CC'TCA_GC
Cuts at: 2691 2691
Size: 5668
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BfrBI ATG'CAT
Cuts at: 2081 2473 2081
Size: 392 5276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 3729 4885 1790
Size: 1939 1156 2573
Fragments arranged by size:
2573 1939 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2230 3399 4357
Size: 416 53 83 399 24 813 1169 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1169 958 813 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4328 1239
Size: 3089 2579
Bpu10I CC'TnA_GC
Cuts at: 2691 2691
Size: 5668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4737
BseYI C'CCAG_C
Cuts at: 2450 4871 5367 2450
Size: 2421 496 2751
Fragments arranged by size:
2751 2421 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 3729 3814 4885 2646
Size: 1083 85 1071 3429
Fragments arranged by size:
3429 1083 1071 85
BsmI GAATG_Cn'
Cuts at: 2289 2600 3179 3272 2289
Size: 311 579 93 4685
Fragments arranged by size:
4685 579 311 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2165 2165
Size: 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2469 2646 2987 3729 3814 4885 1391
Size: 399 679 177 341 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 399 341 177 85
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2726 3023 844
Size: 1268 614 297 3489
Fragments arranged by size:
3489 1268 614 297
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2241 4163 4337 65
Size: 1183 993 1922 174 1396
Fragments arranged by size:
1922 1396 1183 993 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2597 794
Size: 1803 3865
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2407 2998 3365 3750 3767 5219 5305 5317
Size: 435 591 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 591 435 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2148 513
Size: 552 939 144 4033
Fragments arranged by size:
4033 939 552 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2436 3184 3978 3998 2436
Size: 748 794 20 4106
Fragments arranged by size:
4106 794 748 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2187 2972 2187
Size: 785 4883
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 3115 4004
Size: 54 1425 50 275 18 504 781 889
Cuts at: 4004 5633 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 781 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 2504 2916 3598 1353
Size: 852 299 412 682 3423
Fragments arranged by size:
3423 852 682 412 299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2279 3731 4328 5115 5383 1239
Size: 393 419 228 1452 597 787 268 1524
Fragments arranged by size:
1524 1452 787 597 419 393 268 228
EcoO109I rG'GnC_Cy
Cuts at: 2455 2455
Size: 5668
EcoRI G'AATT_C
Cuts at: 2573 2977 3355 2573
Size: 404 378 4886
Fragments arranged by size:
4886 404 378
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2783 2815 4309 4341 4383
Size: 32 135 32 1211 32 1494 32 42
Cuts at: 4383 4415 1373
Size: 32 2626
Fragments arranged by size:
2626 1494 1211 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2381 3012 3258 669
Size: 988 281 443 631 246 3079
Fragments arranged by size:
3079 988 631 443 281 246
HindIII A'AGCT_T
Cuts at: 748 2063 748
Size: 1315 4353
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2870 4782 4966 1305
Size: 1565 1912 184 2007
Fragments arranged by size:
2007 1912 1565 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2336 10
Size: 54 1425 343 504 3342
Fragments arranged by size:
3342 1425 504 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2348 3626 3985 4144 518
Size: 648 981 201 1278 359 159 2042
Fragments arranged by size:
2042 1278 981 648 359 201 159
MspA1I CmG'CkG
Cuts at: 1606 2690 2759 3084 3761 4909 5154 1606
Size: 1084 69 325 677 1148 245 2120
Fragments arranged by size:
2120 1148 1084 677 325 245 69
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4177
Fragments arranged by size:
4177 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NsiI A_TGCA'T
Cuts at: 2083 2475 2083
Size: 392 5276
NspI r_CATG'y
Cuts at: 1132 1229 2081 2429 2477 3026 4571 1132
Size: 97 852 348 48 549 1545 2229
Fragments arranged by size:
2229 1545 852 549 348 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2157 2705 1613
Size: 544 548 4576
Fragments arranged by size:
4576 548 544
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 2617 2764 3020 830
Size: 1787 147 256 3478
Fragments arranged by size:
3478 1787 256 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2690 2759 2690
Size: 69 5599
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SapI GCTCTTCn'nnn_
Cuts at: 2916 2916
Size: 5668
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2419 2613 2667 2760 3016 4151 4832
Size: 219 1374 194 54 93 256 1135 681
Cuts at: 4832 5023 826
Size: 191 1471
Fragments arranged by size:
1471 1374 1135 681 256 219 194 191
93 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 2429 2477 3026 1132
Size: 1297 48 549 3774
Fragments arranged by size:
3774 1297 549 48
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2004 2703 3107 513
Size: 552 939 699 404 3074
Fragments arranged by size:
3074 939 699 552 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2146
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 2925 3797 2925
Size: 872 4796
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI