Restriction map for
pFN21AE5077
(Circular) (Six-base) MAPSORT of: pFN21AE5077.seq Check: 624 from: 1 to: 5302
pFN21AE5077 5302 bp
With 182 enzymes: SgfI *
September 8, 2014 18:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3112 278
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 1937
Size: 708 4594
Acc65I G'GTAC_C
Cuts at: 2623 2623
Size: 5302
AclI AA'CG_TT
Cuts at: 1563 3429 3802 1563
Size: 1866 373 3063
Fragments arranged by size:
3063 1866 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2188 3365 4749 5017 1632
Size: 556 1177 1384 268 1917
Fragments arranged by size:
1917 1384 1177 556 268
AflII C'TTAA_G
Cuts at: 820 1017 2390 820
Size: 197 1373 3732
Fragments arranged by size:
3732 1373 197
AflIII A'CryG_T
Cuts at: 1203 4201 5178 1203
Size: 2998 977 1327
Fragments arranged by size:
2998 1327 977
AgeI A'CCGG_T
Cuts at: 4133 4133
Size: 5302
AhdI GACnn_n'nnGTC
Cuts at: 4031 4031
Size: 5302
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5302
AlwNI CAG_nnn'CTG
Cuts at: 2211 3045 4617 2211
Size: 834 1572 2896
Fragments arranged by size:
2896 1572 834
ApaLI G'TGCA_C
Cuts at: 2111 3359 4515 2111
Size: 1248 1156 2898
Fragments arranged by size:
2898 1248 1156
ApoI r'AATT_y
Cuts at: 1493 2611 2837 2989 5098 1493
Size: 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3856 160
Size: 872 2824 1606
Fragments arranged by size:
2824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2567 2627 1870
Size: 73 128 496 60 4545
Fragments arranged by size:
4545 496 128 73 60
AvrII C'CTAG_G
Cuts at: 2521 2521
Size: 5302
BamHI G'GATC_C
Cuts at: 2437 2632 2999 2437
Size: 195 367 4740
Fragments arranged by size:
4740 367 195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2098 2623 4078 618
Size: 325 217 627 251 60 525 1455 1842
Fragments arranged by size:
1842 1455 627 525 325 251 217 60
BanII G_rGCy'C
Cuts at: 1391 2078 2621 1391
Size: 687 543 4072
Fragments arranged by size:
4072 687 543
BbeI G_GCGC'C
Cuts at: 2042 2102 2042
Size: 60 5242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2194 928
Size: 971 295 4036
Fragments arranged by size:
4036 971 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3493 3527 1903
Size: 34 1556 34 3678
Fragments arranged by size:
3678 1556 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4404 3194
Size: 1210 4092
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 2271 3913
Size: 107 122 71 631 605 502 97 1642
Cuts at: 3913 136
Size: 1525
Fragments arranged by size:
1642 1525 631 605 502 122 107 97
71
BglII A'GATC_T
Cuts at: 5297 5297
Size: 5302
BlpI GC'TnA_GC
Cuts at: 2719 2719
Size: 5302
Bme1580I G_kGCm'C
Cuts at: 1790 2115 2403 3363 4519 1790
Size: 325 288 960 1156 2573
Fragments arranged by size:
2573 1156 960 325 288
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3033 3991 442
Size: 416 53 83 399 24 1616 958 1753
Fragments arranged by size:
1753 1616 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 3962 1239
Size: 1320 1403 2579
Fragments arranged by size:
2579 1403 1320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 3427 4292 4590 2240
Size: 1187 865 298 2952
Fragments arranged by size:
2952 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 882
Size: 617 2466 2219
Fragments arranged by size:
2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2241 3093 493
Size: 711 1037 852 2702
Fragments arranged by size:
2702 1037 852 711
BsaBI GATnn'nnATC
Cuts at: 5296 5296
Size: 5302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 2099 2357 3109
Size: 53 83 186 936 506 60 258 752
Cuts at: 3109 3491 4126 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 752 635 506 382 258 186
83 60 53
BsaWI w'CCGG_w
Cuts at: 2142 2301 3735 4133 4407 4554 4965 2142
Size: 159 1434 398 274 147 411 2479
Fragments arranged by size:
2479 1434 411 398 274 159 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5077
Fragments arranged by size:
5077 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2530 1186
Size: 1344 3958
BseYI C'CCAG_C
Cuts at: 4505 5001 4505
Size: 496 4806
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 1163
Size: 573 525 4204
Fragments arranged by size:
4204 573 525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2218 2373 2601 2752 3513 4541
Size: 275 186 217 155 228 151 761 1028
Cuts at: 4541 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 275 228 217 186 155
151
BsiHKAI G_wGCw'C
Cuts at: 2115 3363 3448 4519 2115
Size: 1248 85 1071 2898
Fragments arranged by size:
2898 1248 1071 85
BsiWI C'GTAC_G
Cuts at: 2308 2308
Size: 5302
BsmI GAATG_Cn'
Cuts at: 2813 2906 2813
Size: 93 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2115 2403 2621 3363 3448 4519
Size: 399 288 37 288 218 742 85 1071
Cuts at: 4519 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 288 288 218 85
37
BspEI T'CCGG_A
Cuts at: 2142 4965 2142
Size: 2823 2479
BspHI T'CATG_A
Cuts at: 3191 4921 3191
Size: 1730 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 844
Size: 1813 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3189 1394
Size: 429 1366 3507
Fragments arranged by size:
3507 1366 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3797 3971 65
Size: 1183 2549 174 1396
Fragments arranged by size:
2549 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2368 3946 4133 1700
Size: 110 558 1578 187 2869
Fragments arranged by size:
2869 1578 558 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5302
BssHII G'CGCG_C
Cuts at: 1925 2191 1925
Size: 266 5036
BssSI C'ACGA_G
Cuts at: 3362 4374 3362
Size: 1012 4290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5302
BstBI TT'CG_AA
Cuts at: 4106 4106
Size: 5302
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5302
BstYI r'GATC_y
Cuts at: 1972 2139 2250 2437 2632 2999 3384 3401 4853
Size: 167 111 187 195 367 385 17 1452
Cuts at: 4853 4939 4951 5297 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 195 187 167
111 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3811
Fragments arranged by size:
3811 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4563
Fragments arranged by size:
4563 683 56
BtsI GCAGTG_nn'
Cuts at: 2818 3612 3632 2818
Size: 794 20 4488
Fragments arranged by size:
4488 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5267
DraI TTT'AAA
Cuts at: 2606 2606
Size: 5302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4309 809
Size: 507 2993 1802
Fragments arranged by size:
2993 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2370 2598
Size: 54 1425 50 275 18 385 155 228
Cuts at: 2598 2749 3638 5267 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 889 385 275 228 155 151
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2215 2370 2598 2749 1537
Size: 275 403 155 228 151 4090
Fragments arranged by size:
4090 403 275 228 155 151
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 3232 1353
Size: 675 1204 3423
Fragments arranged by size:
3423 1204 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3889 4263 4409 387
Size: 1064 138 2300 374 146 1280
Fragments arranged by size:
2300 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2559 3365 3962 4749 5017 1239
Size: 393 556 371 806 597 787 268 1524
Fragments arranged by size:
1524 806 787 597 556 393 371 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2279
Size: 5302
EcoRI G'AATT_C
Cuts at: 2611 2989 2611
Size: 378 4924
FspI TGC'GCA
Cuts at: 3808 3808
Size: 5302
HaeII r_GCGC'y
Cuts at: 1678 2042 2102 2263 4449 1678
Size: 364 60 161 2186 2531
Fragments arranged by size:
2531 2186 364 161 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3943 3975 4017 4049 1373
Size: 32 135 32 2371 32 42 32 2626
Fragments arranged by size:
2626 2371 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 2892 669
Size: 988 281 708 246 3079
Fragments arranged by size:
3079 988 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5302
HpaI GTT'AAC
Cuts at: 2892 2892
Size: 5302
KasI G'GCGC_C
Cuts at: 2038 2098 2038
Size: 60 5242
KpnI G_GTAC'C
Cuts at: 2627 2627
Size: 5302
MfeI C'AATT_G
Cuts at: 2901 2901
Size: 5302
MluI A'CGCG_T
Cuts at: 5178 5178
Size: 5302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4416 4600 1305
Size: 3111 184 2007
Fragments arranged by size:
3111 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3480
Fragments arranged by size:
3480 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3260 3619 3778 518
Size: 648 2094 359 159 2042
Fragments arranged by size:
2094 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2208 2537 2718 3395 4543 4788 1606
Size: 439 163 329 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 439 329 245 181 163
NaeI GCC'GGC
Cuts at: 2370 2370
Size: 5302
NarI GG'CG_CC
Cuts at: 2039 2099 2039
Size: 60 5242
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3811
Fragments arranged by size:
3811 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5302
NgoMIV G'CCGG_C
Cuts at: 2368 2368
Size: 5302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5302
NotI GC'GGCC_GC
Cuts at: 2749 2749
Size: 5302
NspI r_CATG'y
Cuts at: 1132 1229 2119 2660 4205 1132
Size: 97 890 541 1545 2229
Fragments arranged by size:
2229 1545 890 541 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5302
PciI A'CATG_T
Cuts at: 4201 4201
Size: 5302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5302
PmeI GTTT'AAAC
Cuts at: 2606 2606
Size: 5302
PmlI CAC'GTG
Cuts at: 2241 2241
Size: 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4884 4916 3371
Size: 32 1481 32 3757
Fragments arranged by size:
3757 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5302
PsiI TTA'TAA
Cuts at: 2872 2872
Size: 5302
PstI C_TGCA'G
Cuts at: 830 2654 830
Size: 1824 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5302
PvuII CAG'CTG
Cuts at: 2537 2537
Size: 5302
SalI G'TCGA_C
Cuts at: 1936 2644 1936
Size: 708 4594
SbfI CC_TGCA'GG
Cuts at: 2654 2654
Size: 5302
ScaI AGT'ACT
Cuts at: 3550 5262 3550
Size: 1712 3590
SexAI A'CCwGG_T
Cuts at: 2280 2280
Size: 5302
SfcI C'TryA_G
Cuts at: 826 1045 2650 3785 4466 4657 826
Size: 219 1605 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2040 2100 2040
Size: 60 5242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5302
SmaI CCC'GGG
Cuts at: 1872 2073 2629 1872
Size: 201 556 4545
Fragments arranged by size:
4545 556 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 2390 3406 4307 4569 820
Size: 197 926 312 135 1016 901 262 1553
Fragments arranged by size:
1553 1016 926 901 312 262 197 135
SnaBI TAC'GTA
Cuts at: 493 3093 493
Size: 2600 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5302
SphI G_CATG'C
Cuts at: 1132 2660 1132
Size: 1528 3774
SrfI GCCC'GGGC
Cuts at: 2073 2073
Size: 5302
SspI AAT'ATT
Cuts at: 5 52 3226 5
Size: 47 3174 2081
Fragments arranged by size:
3174 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5302
StyI C'CwwG_G
Cuts at: 513 1065 2004 2424 2521 2741 513
Size: 552 939 420 97 220 3074
Fragments arranged by size:
3074 939 552 420 220 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3329 3346 3499 3684 4129 325
Size: 1255 1749 17 153 185 445 1498
Fragments arranged by size:
1749 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3548 5260 96
Size: 274 80 33 51 1443 1571 1712 138
Fragments arranged by size:
1712 1571 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5262 5294 5262
Size: 32 5270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5302
XbaI T'CTAG_A
Cuts at: 2638 2638
Size: 5302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 2431 2170
Size: 261 5041
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5302
XmaI C'CCGG_G
Cuts at: 1870 2071 2627 1870
Size: 201 556 4545
Fragments arranged by size:
4545 556 201
XmnI GAAnn'nnTTC
Cuts at: 3431 3431
Size: 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3110 276
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SwaI