Restriction map for
pFN21AE5074
(Circular) (Six-base) MAPSORT of: pFN21AE5074.seq Check: 3460 from: 1 to: 5233
pFN21AE5074 5233 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3043 278
Size: 53 83 186 936 1507 2468
Fragments arranged by size:
2468 1507 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 2576 1937
Size: 370 269 4594
Fragments arranged by size:
4594 370 269
Acc65I G'GTAC_C
Cuts at: 2554 2554
Size: 5233
AclI AA'CG_TT
Cuts at: 1563 3360 3733 1563
Size: 1797 373 3063
Fragments arranged by size:
3063 1797 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3296 4680 4948 1632
Size: 1664 1384 268 1917
Fragments arranged by size:
1917 1664 1384 268
AfeI AGC'GCT
Cuts at: 2410 2410
Size: 5233
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5036
AflIII A'CryG_T
Cuts at: 1203 4132 5109 1203
Size: 2929 977 1327
Fragments arranged by size:
2929 1327 977
AgeI A'CCGG_T
Cuts at: 4064 4064
Size: 5233
AhdI GACnn_n'nnGTC
Cuts at: 3962 3962
Size: 5233
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5233
AlwNI CAG_nnn'CTG
Cuts at: 2021 2976 4548 2021
Size: 955 1572 2706
Fragments arranged by size:
2706 1572 955
ApaI G_GGCC'C
Cuts at: 2525 2525
Size: 5233
ApaLI G'TGCA_C
Cuts at: 3290 4446 3290
Size: 1156 4077
ApoI r'AATT_y
Cuts at: 1493 2542 2768 2920 5029 1493
Size: 1049 226 152 2109 1697
Fragments arranged by size:
2109 1697 1049 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3787 160
Size: 872 2755 1606
Fragments arranged by size:
2755 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5233
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2434 2558 1870
Size: 73 144 347 124 4545
Fragments arranged by size:
4545 347 144 124 73
BamHI G'GATC_C
Cuts at: 2563 2930 2563
Size: 367 4866
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2294 2364 2554 4009
Size: 325 217 627 238 269 70 190 1455
Cuts at: 4009 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 269 238 217 190
70
BanII G_rGCy'C
Cuts at: 1391 2256 2505 2525 2552 1391
Size: 865 249 20 27 4072
Fragments arranged by size:
4072 865 249 27 20
BbeI G_GCGC'C
Cuts at: 2029 2298 2029
Size: 269 4964
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4262
BbvCI CC'TCA_GC
Cuts at: 2229 2375 2229
Size: 146 5087
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3424 3458 1903
Size: 34 1487 34 3678
Fragments arranged by size:
3678 1487 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3125 4335 3125
Size: 1210 4023
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5233
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2273 2477 3844
Size: 107 122 71 631 605 601 204 1367
Cuts at: 3844 136
Size: 1525
Fragments arranged by size:
1525 1367 631 605 601 204 122 107
71
BglII A'GATC_T
Cuts at: 5228 5228
Size: 5233
BlpI GC'TnA_GC
Cuts at: 2650 2650
Size: 5233
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2525 3294 4450 1790
Size: 421 314 769 1156 2573
Fragments arranged by size:
2573 1156 769 421 314
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5233
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2476 2964 3922
Size: 416 53 83 399 24 1059 488 958
Cuts at: 3922 442
Size: 1753
Fragments arranged by size:
1753 1059 958 488 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2438 2459 3893 1239
Size: 1031 168 21 1434 2579
Fragments arranged by size:
2579 1434 1031 168 21
Bpu10I CC'TnA_GC
Cuts at: 2229 2375 2229
Size: 146 5087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3358 4223 4521 3358
Size: 865 298 4070
Fragments arranged by size:
4070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2451 3896 882
Size: 617 952 1445 2219
Fragments arranged by size:
2219 1445 952 617
BsaAI yAC'GTr
Cuts at: 493 1204 2259 3024 493
Size: 711 1055 765 2702
Fragments arranged by size:
2702 1055 765 711
BsaBI GATnn'nnATC
Cuts at: 5227 5227
Size: 5233
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2295 3040 3422
Size: 53 83 186 936 493 269 745 382
Cuts at: 3422 4057 275
Size: 635 1451
Fragments arranged by size:
1451 936 745 635 493 382 269 186
83 53
BsaWI w'CCGG_w
Cuts at: 3666 4064 4338 4485 4896 3666
Size: 398 274 147 411 4003
Fragments arranged by size:
4003 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 1178
Size: 30 165 30 840 30 4138
Fragments arranged by size:
4138 840 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2480 1186
Size: 943 351 3939
Fragments arranged by size:
3939 943 351
BseYI C'CCAG_C
Cuts at: 2021 2506 4436 4932 2021
Size: 485 1930 496 2322
Fragments arranged by size:
2322 1930 496 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 1163
Size: 573 593 4067
Fragments arranged by size:
4067 593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2323 2337 2683 3444 4472 1540
Size: 275 186 322 14 346 761 1028 2301
Fragments arranged by size:
2301 1028 761 346 322 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 3294 3379 4450 3294
Size: 85 1071 4077
Fragments arranged by size:
4077 1071 85
BsmI GAATG_Cn'
Cuts at: 2744 2837 2744
Size: 93 5140
BsmBI CGTCTCn'nnnn_
Cuts at: 2161 2161
Size: 5233
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2256 2505 2525 2552 3294 3379
Size: 399 421 45 249 20 27 742 85
Cuts at: 3379 4450 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 249 85 45
27 20
BspEI T'CCGG_A
Cuts at: 4896 4896
Size: 5233
BspHI T'CATG_A
Cuts at: 3122 4852 3122
Size: 1730 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2588 844
Size: 1400 344 3489
Fragments arranged by size:
3489 1400 344
BsrBI CCG'CTC
Cuts at: 1394 1823 2331 3120 1394
Size: 429 508 789 3507
Fragments arranged by size:
3507 789 508 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3728 3902 65
Size: 1183 2480 174 1396
Fragments arranged by size:
2480 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 2288 3877 4064 1700
Size: 110 201 277 1589 187 2869
Fragments arranged by size:
2869 1589 277 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5233
BssHII G'CGCG_C
Cuts at: 1925 2094 2126 2128 2179 2181 2284 1925
Size: 169 32 2 51 2 103 4874
Fragments arranged by size:
4874 169 103 51 32 2 2
BssSI C'ACGA_G
Cuts at: 3293 4305 3293
Size: 1012 4221
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5233
BstBI TT'CG_AA
Cuts at: 4037 4037
Size: 5233
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5233
BstYI r'GATC_y
Cuts at: 1972 2563 2930 3315 3332 4784 4870 4882 5228
Size: 591 367 385 17 1452 86 12 346
Cuts at: 5228 1972
Size: 1977
Fragments arranged by size:
1977 1452 591 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2512 2526 2512
Size: 14 5219
BtgI C'CryG_G
Cuts at: 513 1065 2004 2029 2103 2268 2277 2368 513
Size: 552 939 25 74 165 9 91 3378
Fragments arranged by size:
3378 939 552 165 91 74 25 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4494
Fragments arranged by size:
4494 683 56
BtsI GCAGTG_nn'
Cuts at: 2749 3543 3563 2749
Size: 794 20 4419
Fragments arranged by size:
4419 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5198
DraI TTT'AAA
Cuts at: 2537 2537
Size: 5233
DraIII CAC_nnn'GTG
Cuts at: 2145 2145
Size: 5233
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4240 809
Size: 507 2924 1802
Fragments arranged by size:
2924 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2320 2334 2680
Size: 54 1425 50 275 18 490 14 346
Cuts at: 2680 3569 5198 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 490 346 275 54 50
43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 2320 2334 2680 1537
Size: 275 508 14 346 4090
Fragments arranged by size:
4090 508 346 275 14
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2399 3163 1353
Size: 734 312 764 3423
Fragments arranged by size:
3423 764 734 312
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3820 4194 4340 387
Size: 1064 138 2231 374 146 1280
Fragments arranged by size:
2231 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2438 2459 3296 3893 4680 4948
Size: 393 638 168 21 837 597 787 268
Cuts at: 4948 1239
Size: 1524
Fragments arranged by size:
1524 837 787 638 597 393 268 168
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2384
Size: 5233
EcoO109I rG'GnC_Cy
Cuts at: 2233 2470 2522 2233
Size: 237 52 4944
Fragments arranged by size:
4944 237 52
EcoRI G'AATT_C
Cuts at: 2542 2920 2542
Size: 378 4855
FspI TGC'GCA
Cuts at: 3739 3739
Size: 5233
HaeII r_GCGC'y
Cuts at: 1678 2029 2298 2412 4380 1678
Size: 351 269 114 1968 2531
Fragments arranged by size:
2531 1968 351 269 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3874 3906 3948 3980 1373
Size: 32 135 32 2302 32 42 32 2626
Fragments arranged by size:
2626 2302 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2152 2577 2823 669
Size: 988 281 214 425 246 3079
Fragments arranged by size:
3079 988 425 281 246 214
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5233
HpaI GTT'AAC
Cuts at: 2823 2823
Size: 5233
KasI G'GCGC_C
Cuts at: 2025 2294 2025
Size: 269 4964
KpnI G_GTAC'C
Cuts at: 2558 2558
Size: 5233
MfeI C'AATT_G
Cuts at: 2832 2832
Size: 5233
MluI A'CGCG_T
Cuts at: 5109 5109
Size: 5233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4347 4531 1305
Size: 3042 184 2007
Fragments arranged by size:
3042 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3411
Fragments arranged by size:
3411 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3191 3550 3709 518
Size: 648 2025 359 159 2042
Fragments arranged by size:
2042 2025 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2105 2122 2279 2370 2649 3326 4474
Size: 425 74 17 157 91 279 677 1148
Cuts at: 4474 4719 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 425 279 245 157 91
74 17
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 5233
NarI GG'CG_CC
Cuts at: 2026 2295 2026
Size: 269 4964
NcoI C'CATG_G
Cuts at: 513 1065 2004 2268 513
Size: 552 939 264 3478
Fragments arranged by size:
3478 939 552 264
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5233
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 5233
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5233
NotI GC'GGCC_GC
Cuts at: 2680 2680
Size: 5233
NspI r_CATG'y
Cuts at: 1132 1229 2591 4136 1132
Size: 97 1362 1545 2229
Fragments arranged by size:
2229 1545 1362 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5233
PciI A'CATG_T
Cuts at: 4132 4132
Size: 5233
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5233
PmeI GTTT'AAAC
Cuts at: 2537 2537
Size: 5233
PmlI CAC'GTG
Cuts at: 2259 2259
Size: 5233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 4815 4847 3302
Size: 32 1481 32 3688
Fragments arranged by size:
3688 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2233
Size: 5233
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5233
PsiI TTA'TAA
Cuts at: 2803 2803
Size: 5233
PspOMI G'GGCC_C
Cuts at: 2521 2521
Size: 5233
PstI C_TGCA'G
Cuts at: 830 2496 2585 830
Size: 1666 89 3478
Fragments arranged by size:
3478 1666 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5233
PvuII CAG'CTG
Cuts at: 2122 2122
Size: 5233
SacII CC_GC'GG
Cuts at: 2032 2106 2280 2371 2032
Size: 74 174 91 4894
Fragments arranged by size:
4894 174 91 74
SalI G'TCGA_C
Cuts at: 1936 2575 1936
Size: 639 4594
SapI GCTCTTCn'nnn_
Cuts at: 2399 2399
Size: 5233
SbfI CC_TGCA'GG
Cuts at: 2496 2585 2496
Size: 89 5144
ScaI AGT'ACT
Cuts at: 3481 5193 3481
Size: 1712 3521
SfcI C'TryA_G
Cuts at: 826 1045 2492 2581 3716 4397 4588 826
Size: 219 1447 89 1135 681 191 1471
Fragments arranged by size:
1471 1447 1135 681 219 191 89
SfiI GGCCn_nnn'nGGCC
Cuts at: 2477 2477
Size: 5233
SfoI GGC'GCC
Cuts at: 2027 2296 2027
Size: 269 4964
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5233
SgrAI Cr'CCGG_yG
Cuts at: 1700 2288 1700
Size: 588 4645
SmaI CCC'GGG
Cuts at: 1872 2436 2560 1872
Size: 564 124 4545
Fragments arranged by size:
4545 564 124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3337 4238 4500 820
Size: 197 926 1394 901 262 1553
Fragments arranged by size:
1553 1394 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3024 493
Size: 2531 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5233
SphI G_CATG'C
Cuts at: 1132 2591 1132
Size: 1459 3774
SspI AAT'ATT
Cuts at: 5 52 3157 5
Size: 47 3105 2081
Fragments arranged by size:
3105 2081 47
StuI AGG'CCT
Cuts at: 1517 2390 1517
Size: 873 4360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2672 513
Size: 552 939 264 404 3074
Fragments arranged by size:
3074 939 552 404 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3260 3277 3430 3615 4060 325
Size: 1255 1680 17 153 185 445 1498
Fragments arranged by size:
1680 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3479 5191 96
Size: 274 80 33 51 1443 1502 1712 138
Fragments arranged by size:
1712 1502 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 5193 5225 2243
Size: 32 2918 32 2251
Fragments arranged by size:
2918 2251 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5233
XbaI T'CTAG_A
Cuts at: 2569 2569
Size: 5233
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5233
XmaI C'CCGG_G
Cuts at: 1870 2434 2558 1870
Size: 564 124 4545
Fragments arranged by size:
4545 564 124
XmnI GAAnn'nnTTC
Cuts at: 2390 3362 2390
Size: 972 4261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3041 276
Size: 53 83 186 936 1507 2468
Fragments arranged by size:
2468 1507 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI
SexAI SrfI SwaI XcmI