Restriction map for
pFN21AE5073
(Circular) (Six-base) MAPSORT of: pFN21AE5073.seq Check: 9422 from: 1 to: 5092
pFN21AE5073 5092 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2141 2141
Size: 5092
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2902 278
Size: 53 83 186 936 1366 2468
Fragments arranged by size:
2468 1366 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2435 1937
Size: 498 4594
Acc65I G'GTAC_C
Cuts at: 2413 2413
Size: 5092
AclI AA'CG_TT
Cuts at: 1563 3219 3592 1563
Size: 1656 373 3063
Fragments arranged by size:
3063 1656 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3155 4539 4807 1632
Size: 1523 1384 268 1917
Fragments arranged by size:
1917 1523 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4895
AflIII A'CryG_T
Cuts at: 1203 3991 4968 1203
Size: 2788 977 1327
Fragments arranged by size:
2788 1327 977
AgeI A'CCGG_T
Cuts at: 3923 3923
Size: 5092
AhdI GACnn_n'nnGTC
Cuts at: 3821 3821
Size: 5092
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5092
AlwNI CAG_nnn'CTG
Cuts at: 2835 4407 2835
Size: 1572 3520
ApaLI G'TGCA_C
Cuts at: 2089 3149 4305 2089
Size: 1060 1156 2876
Fragments arranged by size:
2876 1156 1060
ApoI r'AATT_y
Cuts at: 1493 2401 2627 2779 4888 1493
Size: 908 226 152 2109 1697
Fragments arranged by size:
2109 1697 908 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3646 160
Size: 872 2614 1606
Fragments arranged by size:
2614 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5092
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 1870
Size: 73 474 4545
Fragments arranged by size:
4545 474 73
BamHI G'GATC_C
Cuts at: 2422 2789 2422
Size: 367 4725
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2327 2360 2413 3868
Size: 325 217 627 525 15 33 53 1455
Cuts at: 3868 618
Size: 1842
Fragments arranged by size:
1842 1455 627 525 325 217 53 33
15
BanII G_rGCy'C
Cuts at: 1391 2303 2411 1391
Size: 912 108 4072
Fragments arranged by size:
4072 912 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2354 2388 3283 3317 1903
Size: 34 417 34 895 34 3678
Fragments arranged by size:
3678 895 417 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2183 2984 4194 2183
Size: 801 1210 3081
Fragments arranged by size:
3081 1210 801
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2103 3703 136
Size: 107 122 71 631 605 431 1600 1525
Fragments arranged by size:
1600 1525 631 605 431 122 107 71
BglII A'GATC_T
Cuts at: 5087 5087
Size: 5092
BlpI GC'TnA_GC
Cuts at: 2509 2509
Size: 5092
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2330 3153 4309 1790
Size: 303 237 823 1156 2573
Fragments arranged by size:
2573 1156 823 303 237
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5092
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2823 3781 442
Size: 416 53 83 399 24 1406 958 1753
Fragments arranged by size:
1753 1406 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 3752 1239
Size: 976 1537 2579
Fragments arranged by size:
2579 1537 976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2287 3217 4082 4380 2287
Size: 930 865 298 2999
Fragments arranged by size:
2999 930 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2222 2360 3755 882
Size: 617 723 138 1395 2219
Fragments arranged by size:
2219 1395 723 617 138
BsaAI yAC'GTr
Cuts at: 493 1204 2883 493
Size: 711 1679 2702
Fragments arranged by size:
2702 1679 711
BsaBI GATnn'nnATC
Cuts at: 5086 5086
Size: 5092
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2899 3281 3916 275
Size: 53 83 186 936 1366 382 635 1451
Fragments arranged by size:
1451 1366 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3525 3923 4197 4344 4755 3525
Size: 398 274 147 411 3862
Fragments arranged by size:
3862 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 1178
Size: 30 165 30 705 30 4132
Fragments arranged by size:
4132 705 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5092
BseYI C'CCAG_C
Cuts at: 2055 2168 2218 4295 4791 2055
Size: 113 50 2077 496 2356
Fragments arranged by size:
2356 2077 496 113 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2542 3303 4331 1540
Size: 275 186 541 761 1028 2301
Fragments arranged by size:
2301 1028 761 541 275 186
BsiHKAI G_wGCw'C
Cuts at: 2093 2303 3153 3238 4309 2093
Size: 210 850 85 1071 2876
Fragments arranged by size:
2876 1071 850 210 85
BsmI GAATG_Cn'
Cuts at: 2603 2696 2603
Size: 93 4999
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2303 2330 2411 3153 3238 4309
Size: 399 303 210 27 81 742 85 1071
Cuts at: 4309 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 303 210 85 81
27
BspEI T'CCGG_A
Cuts at: 4755 4755
Size: 5092
BspHI T'CATG_A
Cuts at: 2981 4711 2981
Size: 1730 3362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 2447 844
Size: 1297 306 3489
Fragments arranged by size:
3489 1297 306
BsrBI CCG'CTC
Cuts at: 1394 1823 2979 1394
Size: 429 1156 3507
Fragments arranged by size:
3507 1156 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3587 3761 65
Size: 1183 2339 174 1396
Fragments arranged by size:
2339 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3736 3923 1700
Size: 110 1926 187 2869
Fragments arranged by size:
2869 1926 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5092
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5092
BssSI C'ACGA_G
Cuts at: 3152 4164 3152
Size: 1012 4080
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5092
BstBI TT'CG_AA
Cuts at: 3896 3896
Size: 5092
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5092
BstXI CCAn_nnnn'nTGG
Cuts at: 2081 2081
Size: 5092
BstYI r'GATC_y
Cuts at: 1972 2422 2789 3174 3191 4643 4729 4741 5087
Size: 450 367 385 17 1452 86 12 346
Cuts at: 5087 1972
Size: 1977
Fragments arranged by size:
1977 1452 450 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2074 513
Size: 552 1009 3531
Fragments arranged by size:
3531 1009 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4353
Fragments arranged by size:
4353 683 56
BtsI GCAGTG_nn'
Cuts at: 2608 3402 3422 2608
Size: 794 20 4278
Fragments arranged by size:
4278 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5092
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5057
DraI TTT'AAA
Cuts at: 2396 2396
Size: 5092
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4099 809
Size: 507 2783 1802
Fragments arranged by size:
2783 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2539 3428 5057
Size: 54 1425 50 275 18 709 889 1629
Cuts at: 5057 8
Size: 43
Fragments arranged by size:
1629 1425 889 709 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2539 1537
Size: 275 727 4090
Fragments arranged by size:
4090 727 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3022 1353
Size: 1669 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3679 4053 4199 387
Size: 1064 138 2090 374 146 1280
Fragments arranged by size:
2090 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2301 2301
Size: 5092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 3155 3752 4539 4807 1239
Size: 393 583 940 597 787 268 1524
Fragments arranged by size:
1524 940 787 597 583 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2096 2096
Size: 5092
EcoRI G'AATT_C
Cuts at: 2401 2779 2401
Size: 378 4714
FspI TGC'GCA
Cuts at: 3598 3598
Size: 5092
HaeII r_GCGC'y
Cuts at: 1678 2242 4239 1678
Size: 564 1997 2531
Fragments arranged by size:
2531 1997 564
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3733 3765 3807 3839 1373
Size: 32 135 32 2161 32 42 32 2626
Fragments arranged by size:
2626 2161 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2436 2682 669
Size: 988 281 498 246 3079
Fragments arranged by size:
3079 988 498 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5092
HpaI GTT'AAC
Cuts at: 2682 2682
Size: 5092
KpnI G_GTAC'C
Cuts at: 2417 2417
Size: 5092
MfeI C'AATT_G
Cuts at: 2691 2691
Size: 5092
MluI A'CGCG_T
Cuts at: 4968 4968
Size: 5092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4206 4390 1305
Size: 2901 184 2007
Fragments arranged by size:
2901 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3270
Fragments arranged by size:
3270 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 3050 3409 3568 518
Size: 648 913 971 359 159 2042
Fragments arranged by size:
2042 971 913 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2172 2310 2508 3185 4333 4578 1606
Size: 566 138 198 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 566 245 198 138
NcoI C'CATG_G
Cuts at: 513 1065 2074 513
Size: 552 1009 3531
Fragments arranged by size:
3531 1009 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5092
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5092
NotI GC'GGCC_GC
Cuts at: 2539 2539
Size: 5092
NspI r_CATG'y
Cuts at: 1132 1229 2450 3995 1132
Size: 97 1221 1545 2229
Fragments arranged by size:
2229 1545 1221 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5092
PciI A'CATG_T
Cuts at: 3991 3991
Size: 5092
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5092
PfoI T'CCnGG_A
Cuts at: 2126 2126
Size: 5092
PmeI GTTT'AAAC
Cuts at: 2396 2396
Size: 5092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 4674 4706 3161
Size: 32 1481 32 3547
Fragments arranged by size:
3547 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5092
PsiI TTA'TAA
Cuts at: 2662 2662
Size: 5092
PstI C_TGCA'G
Cuts at: 830 2444 830
Size: 1614 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5092
SacI G_AGCT'C
Cuts at: 2303 2303
Size: 5092
SalI G'TCGA_C
Cuts at: 1936 2434 1936
Size: 498 4594
SbfI CC_TGCA'GG
Cuts at: 2444 2444
Size: 5092
ScaI AGT'ACT
Cuts at: 3340 5052 3340
Size: 1712 3380
SfcI C'TryA_G
Cuts at: 826 1045 2440 3575 4256 4447 826
Size: 219 1395 1135 681 191 1471
Fragments arranged by size:
1471 1395 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2103 2103
Size: 5092
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5092
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5092
SmaI CCC'GGG
Cuts at: 1872 2419 1872
Size: 547 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2302 3196 4097 4359 820
Size: 197 926 359 894 901 262 1553
Fragments arranged by size:
1553 926 901 894 359 262 197
SnaBI TAC'GTA
Cuts at: 493 2883 493
Size: 2390 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5092
SphI G_CATG'C
Cuts at: 1132 2450 1132
Size: 1318 3774
SspI AAT'ATT
Cuts at: 5 52 3016 5
Size: 47 2964 2081
Fragments arranged by size:
2964 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5092
StyI C'CwwG_G
Cuts at: 513 1065 2074 2100 2232 2531 513
Size: 552 1009 26 132 299 3074
Fragments arranged by size:
3074 1009 552 299 132 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3119 3136 3289 3474 3919 325
Size: 1255 1539 17 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3338 5050 96
Size: 274 80 33 51 1443 1361 1712 138
Fragments arranged by size:
1712 1443 1361 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5052 5084 5052
Size: 32 5060
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5092
XbaI T'CTAG_A
Cuts at: 2428 2428
Size: 5092
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5092
XmaI C'CCGG_G
Cuts at: 1870 2417 1870
Size: 547 4545
XmnI GAAnn'nnTTC
Cuts at: 3221 3221
Size: 5092
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2900 276
Size: 53 83 186 936 1366 2468
Fragments arranged by size:
2468 1366 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI
SrfI SwaI XcmI