Restriction map for
pFN21AE5066
(Circular) (Six-base) MAPSORT of: pFN21AE5066.seq Check: 6514 from: 1 to: 7609
pFN21AE5066 7609 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4194 4759 4194
Size: 565 7044
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5419 278
Size: 53 83 186 936 3883 2468
Fragments arranged by size:
3883 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3312 4952 1937
Size: 1375 1640 4594
Fragments arranged by size:
4594 1640 1375
Acc65I G'GTAC_C
Cuts at: 4930 4930
Size: 7609
AclI AA'CG_TT
Cuts at: 1563 4836 4908 5736 6109 1563
Size: 3273 72 828 373 3063
Fragments arranged by size:
3273 3063 828 373 72
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3116 3312 3981 5672 7056 7324 1632
Size: 1484 196 669 1691 1384 268 1917
Fragments arranged by size:
1917 1691 1484 1384 669 268 196
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7412
AflIII A'CryG_T
Cuts at: 1203 6508 7485 1203
Size: 5305 977 1327
Fragments arranged by size:
5305 1327 977
AgeI A'CCGG_T
Cuts at: 6440 6440
Size: 7609
AhdI GACnn_n'nnGTC
Cuts at: 6338 6338
Size: 7609
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7609
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 7577
AlwNI CAG_nnn'CTG
Cuts at: 3073 3202 5352 6924 3073
Size: 129 2150 1572 3758
Fragments arranged by size:
3758 2150 1572 129
ApaI G_GGCC'C
Cuts at: 2552 2552
Size: 7609
ApaLI G'TGCA_C
Cuts at: 5666 6822 5666
Size: 1156 6453
ApoI r'AATT_y
Cuts at: 1493 2476 3192 4918 5144 5296 7405 1493
Size: 983 716 1726 226 152 2109 1697
Fragments arranged by size:
2109 1726 1697 983 716 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3129 6163 160
Size: 872 2097 3034 1606
Fragments arranged by size:
3034 2097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
AvaI C'yCGr_G
Cuts at: 1870 1943 4195 4892 4934 1870
Size: 73 2252 697 42 4545
Fragments arranged by size:
4545 2252 697 73 42
BamHI G'GATC_C
Cuts at: 4939 5306 4939
Size: 367 7242
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2828 4274 4598 4930 6385
Size: 325 217 627 1041 1446 324 332 1455
Cuts at: 6385 618
Size: 1842
Fragments arranged by size:
1842 1455 1446 1041 627 332 325 324
217
BanII G_rGCy'C
Cuts at: 1391 2312 2552 3423 3792 4928 1391
Size: 921 240 871 369 1136 4072
Fragments arranged by size:
4072 1136 921 871 369 240
BbeI G_GCGC'C
Cuts at: 4602 4602
Size: 7609
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3390 3805 4541 928
Size: 971 1491 415 736 3996
Fragments arranged by size:
3996 1491 971 736 415
BbvCI CC'TCA_GC
Cuts at: 2859 4603 2859
Size: 1744 5865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5800 5834 1903
Size: 34 3863 34 3678
Fragments arranged by size:
3863 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4259 5501 6711 4259
Size: 1242 1210 5157
Fragments arranged by size:
5157 1242 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7609
BfrBI ATG'CAT
Cuts at: 3083 3083
Size: 7609
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4289 6220 136
Size: 107 122 71 631 605 2617 1931 1525
Fragments arranged by size:
2617 1931 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2449 2466 2643 3627 7604 2449
Size: 17 177 984 3977 2454
Fragments arranged by size:
3977 2454 984 177 17
BlpI GC'TnA_GC
Cuts at: 2201 3612 5026 2201
Size: 1411 1414 4784
Fragments arranged by size:
4784 1414 1411
Bme1580I G_kGCm'C
Cuts at: 1790 2552 2825 2833 3430 5670 6826 1790
Size: 762 273 8 597 2240 1156 2573
Fragments arranged by size:
2573 2240 1156 762 597 273 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7609
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2853 4423 5340
Size: 416 53 83 399 24 1436 1570 917
Cuts at: 5340 6298 442
Size: 958 1753
Fragments arranged by size:
1753 1570 1436 958 917 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 3458 3466 4604 6269 1239
Size: 1421 798 8 1138 1665 2579
Fragments arranged by size:
2579 1665 1421 1138 798 8
Bpu10I CC'TnA_GC
Cuts at: 2859 3090 3702 4603 2859
Size: 231 612 901 5865
Fragments arranged by size:
5865 901 612 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3137 5734 6599 6897 3137
Size: 2597 865 298 3849
Fragments arranged by size:
3849 2597 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4061 6272 882
Size: 617 2562 2211 2219
Fragments arranged by size:
2562 2219 2211 617
BsaAI yAC'GTr
Cuts at: 493 1204 2076 4704 5400 493
Size: 711 872 2628 696 2702
Fragments arranged by size:
2702 2628 872 711 696
BsaBI GATnn'nnATC
Cuts at: 2280 7603 2280
Size: 5323 2286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2601 3172 4599 5416
Size: 53 83 186 936 1068 571 1427 817
Cuts at: 5416 5798 6433 275
Size: 382 635 1451
Fragments arranged by size:
1451 1427 1068 936 817 635 571 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2583 4251 6042 6440 6714 6861 7272 2583
Size: 1668 1791 398 274 147 411 2920
Fragments arranged by size:
2920 1791 1668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2970 3000 3530 3560 3705
Size: 30 165 30 1567 30 530 30 145
Cuts at: 3705 3735 4200 4230 1178
Size: 30 465 30 4557
Fragments arranged by size:
4557 1567 530 465 165 145 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 3233 3347 3726 1186
Size: 1171 876 114 379 5069
Fragments arranged by size:
5069 1171 876 379 114
BseYI C'CCAG_C
Cuts at: 2237 2305 3348 4881 6812 7308 2237
Size: 68 1043 1533 1931 496 2538
Fragments arranged by size:
2538 1931 1533 1043 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3164 4397 4784 4800 1163
Size: 573 1428 1233 387 16 3972
Fragments arranged by size:
3972 1428 1233 573 387 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2584 5059 5820 6848 1540
Size: 275 186 583 2475 761 1028 2301
Fragments arranged by size:
2475 2301 1028 761 583 275 186
BsiHKAI G_wGCw'C
Cuts at: 3423 5670 5755 6826 3423
Size: 2247 85 1071 4206
Fragments arranged by size:
4206 2247 1071 85
BsmI GAATG_Cn'
Cuts at: 2385 2462 2688 3703 4732 5120 5213 2385
Size: 77 226 1015 1029 388 93 4781
Fragments arranged by size:
4781 1029 1015 388 226 93 77
BsmBI CGTCTCn'nnnn_
Cuts at: 2971 4099 2971
Size: 1128 6481
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 2552 2825 2833 3423 3430 3792
Size: 399 522 240 273 8 590 7 362
Cuts at: 3792 4928 5670 5755 6826 1391
Size: 1136 742 85 1071 2174
Fragments arranged by size:
2174 1136 1071 742 590 522 399 362
273 240 85 8 7
BspEI T'CCGG_A
Cuts at: 4251 7272 4251
Size: 3021 4588
BspHI T'CATG_A
Cuts at: 3807 5498 7228 3807
Size: 1691 1730 4188
Fragments arranged by size:
4188 1730 1691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4194 4759 4964 844
Size: 3350 565 205 3489
Fragments arranged by size:
3489 3350 565 205
BsrBI CCG'CTC
Cuts at: 1394 1823 4596 5496 1394
Size: 429 2773 900 3507
Fragments arranged by size:
3507 2773 900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4236 6104 6278 65
Size: 1183 2988 1868 174 1396
Fragments arranged by size:
2988 1868 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2943 6253 6440 1700
Size: 110 1133 3310 187 2869
Fragments arranged by size:
3310 2869 1133 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7609
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7609
BssSI C'ACGA_G
Cuts at: 5669 6681 5669
Size: 1012 6597
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7609
BstBI TT'CG_AA
Cuts at: 6413 6413
Size: 7609
BstEII G'GTnAC_C
Cuts at: 1184 3746 1184
Size: 2562 5047
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2381 2923 3578 2104
Size: 277 542 655 6135
Fragments arranged by size:
6135 655 542 277
BstYI r'GATC_y
Cuts at: 1972 2449 2466 2571 2643 3271 3627 4939 5306
Size: 477 17 105 72 628 356 1312 367
Cuts at: 5306 5691 5708 7160 7246 7258 7604 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1312 628 477 385 367 356
346 105 86 72 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 4295 4295
Size: 7609
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 513
Size: 552 939 186 5932
Fragments arranged by size:
5932 939 552 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6870
Fragments arranged by size:
6870 683 56
BtsI GCAGTG_nn'
Cuts at: 2572 3231 3673 4413 5125 5919 5939 2572
Size: 659 442 740 712 794 20 4242
Fragments arranged by size:
4242 794 740 712 659 442 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7574
DraI TTT'AAA
Cuts at: 4913 4913
Size: 7609
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2792 6616 809
Size: 507 1476 3824 1802
Fragments arranged by size:
3824 1802 1476 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 4410 4866
Size: 54 1425 50 275 18 1111 1469 456
Cuts at: 4866 5056 5945 7574 8
Size: 190 889 1629 43
Fragments arranged by size:
1629 1469 1425 1111 889 456 275 190
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5056 1537
Size: 275 3244 4090
Fragments arranged by size:
4090 3244 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2706 3132 3288 3324 3411 3549 3957 4640
Size: 1353 426 156 36 87 138 408 683
Cuts at: 4640 4676 5539 1353
Size: 36 863 3423
Fragments arranged by size:
3423 1353 863 683 426 408 156 138
87 36 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2978 6196 6570 6716 387
Size: 1064 138 1389 3218 374 146 1280
Fragments arranged by size:
3218 1389 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3421 3421
Size: 7609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2660 3116 3312 3458 3466 3981 4604
Size: 393 1028 456 196 146 8 515 623
Cuts at: 4604 5672 6269 7056 7324 1239
Size: 1068 597 787 268 1524
Fragments arranged by size:
1524 1068 1028 787 623 597 515 456
393 268 196 146 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3650 4400 4472 4850 3650
Size: 750 72 378 6409
Fragments arranged by size:
6409 750 378 72
EcoO109I rG'GnC_Cy
Cuts at: 2549 3103 3301 4045 4105 4291 4405 4612 2549
Size: 554 198 744 60 186 114 207 5546
Fragments arranged by size:
5546 744 554 207 198 186 114 60
EcoRI G'AATT_C
Cuts at: 4918 5296 4918
Size: 378 7231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 7609
FseI GG_CCGG'CC
Cuts at: 2947 2947
Size: 7609
FspI TGC'GCA
Cuts at: 3533 6115 3533
Size: 2582 5027
HaeII r_GCGC'y
Cuts at: 1678 4602 4859 6756 1678
Size: 2924 257 1897 2531
Fragments arranged by size:
2924 2531 1897 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2578 2610 2970 3002 3612
Size: 32 135 32 1006 32 360 32 610
Cuts at: 3612 3644 4118 4150 6250 6282 6324 6356 1373
Size: 32 474 32 2100 32 42 32 2626
Fragments arranged by size:
2626 2100 1006 610 474 360 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2617 4953 5199 669
Size: 988 281 679 2336 246 3079
Fragments arranged by size:
3079 2336 988 679 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7609
HpaI GTT'AAC
Cuts at: 5199 5199
Size: 7609
KasI G'GCGC_C
Cuts at: 4598 4598
Size: 7609
KpnI G_GTAC'C
Cuts at: 4934 4934
Size: 7609
MfeI C'AATT_G
Cuts at: 2172 5208 2172
Size: 3036 4573
MluI A'CGCG_T
Cuts at: 7485 7485
Size: 7609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2796 6723 6907 1305
Size: 1491 3927 184 2007
Fragments arranged by size:
3927 2007 1491 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4412 4868 10
Size: 54 1425 343 2580 456 2751
Fragments arranged by size:
2751 2580 1425 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2076 2726 2921 5567 5926 6085 518
Size: 648 910 650 195 2646 359 159 2042
Fragments arranged by size:
2646 2042 910 650 648 359 195 159
MspA1I CmG'CkG
Cuts at: 1606 2435 3352 4864 4885 5025 5702 6850 7095
Size: 829 917 1512 21 140 677 1148 245
Cuts at: 7095 1606
Size: 2120
Fragments arranged by size:
2120 1512 1148 917 829 677 245 140
21
NaeI GCC'GGC
Cuts at: 2945 2945
Size: 7609
NarI GG'CG_CC
Cuts at: 4599 4599
Size: 7609
NcoI C'CATG_G
Cuts at: 513 1065 2004 2190 513
Size: 552 939 186 5932
Fragments arranged by size:
5932 939 552 186
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7609
NgoMIV G'CCGG_C
Cuts at: 2943 2943
Size: 7609
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7609
NotI GC'GGCC_GC
Cuts at: 5056 5056
Size: 7609
NsiI A_TGCA'T
Cuts at: 3085 3085
Size: 7609
NspI r_CATG'y
Cuts at: 1132 1229 2349 4967 6512 1132
Size: 97 1120 2618 1545 2229
Fragments arranged by size:
2618 2229 1545 1120 97
PasI CC'CwG_GG
Cuts at: 1348 3073 1348
Size: 1725 5884
PciI A'CATG_T
Cuts at: 6508 6508
Size: 7609
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2843 3073 4758 1108
Size: 1735 230 1685 3959
Fragments arranged by size:
3959 1735 1685 230
PfoI T'CCnGG_A
Cuts at: 3180 3223 4581 3180
Size: 43 1358 6208
Fragments arranged by size:
6208 1358 43
PmeI GTTT'AAAC
Cuts at: 4913 4913
Size: 7609
PmlI CAC'GTG
Cuts at: 4704 4704
Size: 7609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5678 5710 7191 7223 3530
Size: 32 2116 32 1481 32 3916
Fragments arranged by size:
3916 2116 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3103 4105 3103
Size: 1002 6607
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7609
PsiI TTA'TAA
Cuts at: 5179 5179
Size: 7609
PspOMI G'GGCC_C
Cuts at: 2548 2548
Size: 7609
PspXI vC'TCGA_Gb
Cuts at: 4195 4195
Size: 7609
PstI C_TGCA'G
Cuts at: 830 2760 4420 4851 4863 4961 830
Size: 1930 1660 431 12 98 3478
Fragments arranged by size:
3478 1930 1660 431 98 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7609
PvuII CAG'CTG
Cuts at: 2435 3352 4864 4885 2435
Size: 917 1512 21 5159
Fragments arranged by size:
5159 1512 917 21
SacI G_AGCT'C
Cuts at: 3423 3423
Size: 7609
SalI G'TCGA_C
Cuts at: 1936 4951 1936
Size: 3015 4594
SanDI GG'GwC_CC
Cuts at: 4105 4105
Size: 7609
SapI GCTCTTCn'nnn_
Cuts at: 3411 4640 4676 3411
Size: 1229 36 6344
Fragments arranged by size:
6344 1229 36
SbfI CC_TGCA'GG
Cuts at: 4851 4961 4851
Size: 110 7499
ScaI AGT'ACT
Cuts at: 5857 7569 5857
Size: 1712 5897
SexAI A'CCwGG_T
Cuts at: 3645 3645
Size: 7609
SfcI C'TryA_G
Cuts at: 826 1045 2756 4416 4847 4859 4957 6092 6773
Size: 219 1711 1660 431 12 98 1135 681
Cuts at: 6773 6964 826
Size: 191 1471
Fragments arranged by size:
1711 1660 1471 1135 681 431 219 191
98 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 4289 4289
Size: 7609
SfoI GGC'GCC
Cuts at: 4600 4600
Size: 7609
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7609
SmaI CCC'GGG
Cuts at: 1872 4936 1872
Size: 3064 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3152 4195 5713 6614 6876 820
Size: 197 926 1209 1043 1518 901 262 1553
Fragments arranged by size:
1553 1518 1209 1043 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2076 5400 493
Size: 1583 3324 2702
Fragments arranged by size:
3324 2702 1583
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7609
SphI G_CATG'C
Cuts at: 1132 4967 1132
Size: 3835 3774
SspI AAT'ATT
Cuts at: 5 52 5533 5
Size: 47 5481 2081
Fragments arranged by size:
5481 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7609
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 3924 3993 4242 4396 4608
Size: 552 939 186 1734 69 249 154 212
Cuts at: 4608 5048 513
Size: 440 3074
Fragments arranged by size:
3074 1734 939 552 440 249 212 186
154 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2387 2528 3956 4509 4750 5636 5653
Size: 1255 807 141 1428 553 241 886 17
Cuts at: 5653 5806 5991 6436 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1428 1255 886 807 553 445 241
185 153 141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3462 5855 7567
Size: 274 80 33 51 1443 1485 2393 1712
Cuts at: 7567 96
Size: 138
Fragments arranged by size:
2393 1712 1485 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2839 2871 3705 3737 4200 4232 7569 7601 2839
Size: 32 834 32 463 32 3337 32 2847
Fragments arranged by size:
3337 2847 834 463 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7609
XbaI T'CTAG_A
Cuts at: 4945 4945
Size: 7609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2101 2101
Size: 7609
XhoI C'TCGA_G
Cuts at: 1943 4195 1943
Size: 2252 5357
XmaI C'CCGG_G
Cuts at: 1870 4934 1870
Size: 3064 4545
XmnI GAAnn'nnTTC
Cuts at: 2147 2811 3545 4544 4634 5738 2147
Size: 664 734 999 90 1104 4018
Fragments arranged by size:
4018 1104 999 734 664 90
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5417 276
Size: 53 83 186 936 3883 2468
Fragments arranged by size:
3883 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BplI BsiWI BstZ17I DraIII
EcoRV FspAI NruI PacI PsrI RsrII SacII SrfI
SwaI