Restriction map for
pFN21AE5065
(Circular) (Six-base) MAPSORT of: pFN21AE5065.seq Check: 9713 from: 1 to: 6475
pFN21AE5065 6475 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3818 1937
Size: 1881 4594
Acc65I G'GTAC_C
Cuts at: 3081 3796 3081
Size: 715 5760
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3029 4538 5922 6190 1632
Size: 1397 1509 1384 268 1917
Fragments arranged by size:
1917 1509 1397 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 2406 3730 5374 6351 1203
Size: 1203 1324 1644 977 1327
Fragments arranged by size:
1644 1327 1324 1203 977
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 2902 2917 5204 2902
Size: 15 2287 4173
Fragments arranged by size:
4173 2287 15
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6475
AlwNI CAG_nnn'CTG
Cuts at: 4218 5790 4218
Size: 1572 4903
ApaI G_GGCC'C
Cuts at: 2605 2731 2605
Size: 126 6349
ApaLI G'TGCA_C
Cuts at: 2822 4532 5688 2822
Size: 1710 1156 3609
Fragments arranged by size:
3609 1710 1156
ApoI r'AATT_y
Cuts at: 1493 3650 3784 4010 4162 6271 1493
Size: 2157 134 226 152 2109 1697
Fragments arranged by size:
2157 2109 1697 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2285 2300 2485 2885 2907 3800
Size: 73 270 72 15 185 400 22 893
Cuts at: 3800 1870
Size: 4545
Fragments arranged by size:
4545 893 400 270 185 73 72 22
15
BamHI G'GATC_C
Cuts at: 2106 3805 4172 2106
Size: 1699 367 4409
Fragments arranged by size:
4409 1699 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2372 2422 2490 3081 3103
Size: 325 217 627 585 50 68 591 22
Cuts at: 3103 3397 3796 5251 618
Size: 294 399 1455 1842
Fragments arranged by size:
1842 1455 627 591 585 399 325 294
217 68 50 22
BanII G_rGCy'C
Cuts at: 1391 2212 2564 2605 2640 2673 2731 2756 2841
Size: 821 352 41 35 33 58 25 85
Cuts at: 2841 3045 3756 3794 1391
Size: 204 711 38 4072
Fragments arranged by size:
4072 821 711 352 204 85 58 41
38 35 33 25
BbeI G_GCGC'C
Cuts at: 2376 2494 3107 2376
Size: 118 613 5744
Fragments arranged by size:
5744 613 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3138 928
Size: 971 1239 4265
Fragments arranged by size:
4265 1239 971
BbvCI CC'TCA_GC
Cuts at: 2501 2501
Size: 6475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4666 4700 1903
Size: 34 2729 34 3678
Fragments arranged by size:
3678 2729 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4367 5577 4367
Size: 1210 5265
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 3571 5086
Size: 107 122 71 631 605 470 1429 1515
Cuts at: 5086 136
Size: 1525
Fragments arranged by size:
1525 1515 1429 631 605 470 122 107
71
BglII A'GATC_T
Cuts at: 2766 6470 2766
Size: 3704 2771
BlpI GC'TnA_GC
Cuts at: 2558 3892 2558
Size: 1334 5141
Bme1580I G_kGCm'C
Cuts at: 1790 2427 2605 2731 2826 4536 5692 1790
Size: 637 178 126 95 1710 1156 2573
Fragments arranged by size:
2573 1710 1156 637 178 126 95
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3757 4206 5164
Size: 416 53 83 399 24 2340 449 958
Cuts at: 5164 442
Size: 1753
Fragments arranged by size:
2340 1753 958 449 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2855 5135 1239
Size: 1616 2280 2579
Fragments arranged by size:
2579 2280 1616
Bpu10I CC'TnA_GC
Cuts at: 2501 2501
Size: 6475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4600 5465 5763 4600
Size: 865 298 5312
Fragments arranged by size:
5312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2282 3592 5138 882
Size: 617 783 1310 1546 2219
Fragments arranged by size:
2219 1546 1310 783 617
BsaAI yAC'GTr
Cuts at: 493 1204 2409 3733 4266 493
Size: 711 1205 1324 533 2702
Fragments arranged by size:
2702 1324 1205 711 533
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2373 2464 2491 3104
Size: 53 83 186 936 840 91 27 613
Cuts at: 3104 3320 4282 4664 5299 275
Size: 216 962 382 635 1451
Fragments arranged by size:
1451 962 936 840 635 613 382 216
186 91 83 53 27
BsaWI w'CCGG_w
Cuts at: 4908 5306 5580 5727 6138 4908
Size: 398 274 147 411 5245
Fragments arranged by size:
5245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2787 2817 1178
Size: 30 165 30 1384 30 4836
Fragments arranged by size:
4836 1384 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2666 2710 2732 1186
Size: 1045 435 44 22 4929
Fragments arranged by size:
4929 1045 435 44 22
BseYI C'CCAG_C
Cuts at: 2242 2427 2587 2614 2850 5678 6174 2242
Size: 185 160 27 236 2828 496 2543
Fragments arranged by size:
2828 2543 496 236 185 160 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2104 3292 3404 1163
Size: 573 368 1188 112 4234
Fragments arranged by size:
4234 1188 573 368 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2522 2918 2976 3925 4686 5714
Size: 275 186 521 396 58 949 761 1028
Cuts at: 5714 1540
Size: 2301
Fragments arranged by size:
2301 1028 949 761 521 396 275 186
58
BsiHKAI G_wGCw'C
Cuts at: 2535 2555 2640 2826 3039 3756 4536 4621 5692
Size: 20 85 186 213 717 780 85 1071
Cuts at: 5692 2535
Size: 3318
Fragments arranged by size:
3318 1071 780 717 213 186 85 85
20
BsmI GAATG_Cn'
Cuts at: 3986 4079 3986
Size: 93 6382
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2212 2427 2535 2555 2564 2605 2640
Size: 399 422 215 108 20 9 41 35
Cuts at: 2640 2673 2731 2756 2826 2841 3039 3045 3756
Size: 33 58 25 70 15 198 6 711
Cuts at: 3756 3794 4536 4621 5692 1391
Size: 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 711 422 399 215 198
108 85 70 58 41 38 35 33
25 20 15 9 6
BspEI T'CCGG_A
Cuts at: 6138 6138
Size: 6475
BspHI T'CATG_A
Cuts at: 4364 6094 4364
Size: 1730 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2038 2731 2994 3093 3830 844
Size: 1194 693 263 99 737 3489
Fragments arranged by size:
3489 1194 737 693 263 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 3248 4362 1394
Size: 429 269 1156 1114 3507
Fragments arranged by size:
3507 1156 1114 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 4970 5144 65
Size: 1183 3722 174 1396
Fragments arranged by size:
3722 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2025 2032 2913 2971 2975 3100
Size: 110 208 7 7 881 58 4 125
Cuts at: 3100 3125 3322 3517 5119 5306 1700
Size: 25 197 195 1602 187 2869
Fragments arranged by size:
2869 1602 881 208 197 195 187 125
110 58 25 7 7 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 2014 2061 2925 1925
Size: 89 47 864 5475
Fragments arranged by size:
5475 864 89 47
BssSI C'ACGA_G
Cuts at: 2318 3038 3589 4535 5547 2318
Size: 720 551 946 1012 3246
Fragments arranged by size:
3246 1012 946 720 551
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 2367 5279 2367
Size: 2912 3563
BstEII G'GTnAC_C
Cuts at: 1184 3335 1184
Size: 2151 4324
BstYI r'GATC_y
Cuts at: 1972 2106 2766 2808 3000 3495 3805 4172 4557
Size: 134 660 42 192 495 310 367 385
Cuts at: 4557 4574 6026 6112 6124 6470 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 660 495 385 367 346 310
192 134 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 3237 3237
Size: 6475
BtgI C'CryG_G
Cuts at: 513 1065 2004 2200 2269 2673 2747 3352 3471
Size: 552 939 196 69 404 74 605 119
Cuts at: 3471 3499 513
Size: 28 3489
Fragments arranged by size:
3489 939 605 552 404 196 119 74
69 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5736
Fragments arranged by size:
5736 683 56
BtsI GCAGTG_nn'
Cuts at: 3991 4785 4805 3991
Size: 794 20 5661
Fragments arranged by size:
5661 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3714 3749 529
Size: 35 3150 35 3255
Fragments arranged by size:
3255 3150 35 35
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5482 809
Size: 507 4166 1802
Fragments arranged by size:
4166 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 2519 2682
Size: 54 1425 50 275 18 373 316 163
Cuts at: 2682 2915 2973 3349 3468 3515 3544 3922 4811
Size: 233 58 376 119 47 29 378 889
Cuts at: 4811 6440 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 378 376 373 316 275
233 163 119 58 54 50 47 43
29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2519 2915 2973 3922 1537
Size: 275 707 396 58 949 4090
Fragments arranged by size:
4090 949 707 396 275 58
EarI CTCTTCn'nnn_
Cuts at: 1353 4405 1353
Size: 3052 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2501 2993 3152 5062 5436 5582
Size: 1064 138 912 492 159 1910 374 146
Cuts at: 5582 387
Size: 1280
Fragments arranged by size:
1910 1280 1064 912 492 374 159 146
138
EcoICRI GAG'CTC
Cuts at: 2638 3754 2638
Size: 1116 5359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2855 3029 4538 5135 5922 6190 1239
Size: 393 1223 174 1509 597 787 268 1524
Fragments arranged by size:
1524 1509 1223 787 597 393 268 174
EcoNI CCTnn'n_nnAGG
Cuts at: 3290 3290
Size: 6475
EcoO109I rG'GnC_Cy
Cuts at: 2601 2727 2728 2743 2857 2881 2601
Size: 126 1 15 114 24 6195
Fragments arranged by size:
6195 126 114 24 15 1
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 2376 2494 3107 3368 5622 1678
Size: 698 118 613 261 2254 2531
Fragments arranged by size:
2531 2254 698 613 261 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2787 2819 2888 2903 2920
Size: 32 135 32 1215 32 69 15 17
Cuts at: 2920 2935 3284 3316 3655 3687 5116 5148 5190
Size: 15 349 32 339 32 1429 32 42
Cuts at: 5190 5222 1373
Size: 32 2626
Fragments arranged by size:
2626 1429 1215 349 339 135 69 42
32 32 32 32 32 32 32 17
15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2056 2191 3819 4065 669
Size: 988 281 118 135 1628 246 3079
Fragments arranged by size:
3079 1628 988 281 246 135 118
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6475
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KasI G'GCGC_C
Cuts at: 2372 2490 3103 2372
Size: 118 613 5744
Fragments arranged by size:
5744 613 118
KpnI G_GTAC'C
Cuts at: 3085 3800 3085
Size: 715 5760
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3417 3629 5589 5773 1305
Size: 2112 212 1960 184 2007
Fragments arranged by size:
2112 2007 1960 212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3351 10
Size: 54 1425 343 1519 3134
Fragments arranged by size:
3134 1519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4433 4792 4951 518
Size: 648 3267 359 159 2042
Fragments arranged by size:
3267 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2242 2271 2329 2392 2622 2749 3231
Size: 558 78 29 58 63 230 127 482
Cuts at: 3231 3891 4568 5716 5961 1606
Size: 660 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 558 482 245 230
127 78 63 58 29
NaeI GCC'GGC
Cuts at: 2020 2027 2973 2977 3102 3324 3519 2020
Size: 7 946 4 125 222 195 4976
Fragments arranged by size:
4976 946 222 195 125 7 4
NarI GG'CG_CC
Cuts at: 2373 2491 3104 2373
Size: 118 613 5744
Fragments arranged by size:
5744 613 118
NcoI C'CATG_G
Cuts at: 513 1065 2004 2200 3499 513
Size: 552 939 196 1299 3489
Fragments arranged by size:
3489 1299 939 552 196
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NgoMIV G'CCGG_C
Cuts at: 2018 2025 2971 2975 3100 3322 3517 2018
Size: 7 946 4 125 222 195 4976
Fragments arranged by size:
4976 946 222 195 125 7 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 2519 3922 2519
Size: 1403 5072
NspI r_CATG'y
Cuts at: 1132 1229 3833 5378 1132
Size: 97 2604 1545 2229
Fragments arranged by size:
2604 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2598 2785 3190 1348
Size: 1250 187 405 4633
Fragments arranged by size:
4633 1250 405 187
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3022 1108
Size: 1914 4561
PfoI T'CCnGG_A
Cuts at: 2400 2400
Size: 6475
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PmlI CAC'GTG
Cuts at: 3733 3733
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 6057 6089 4544
Size: 32 1481 32 4930
Fragments arranged by size:
4930 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2743 2881 2743
Size: 138 6337
PshAI GACnn'nnGTC
Cuts at: 1613 3537 1613
Size: 1924 4551
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PspOMI G'GGCC_C
Cuts at: 2601 2727 2601
Size: 126 6349
PstI C_TGCA'G
Cuts at: 830 3827 830
Size: 2997 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2242 3231 2242
Size: 989 5486
SacI G_AGCT'C
Cuts at: 2640 3756 2640
Size: 1116 5359
SacII CC_GC'GG
Cuts at: 2272 2750 2272
Size: 478 5997
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SbfI CC_TGCA'GG
Cuts at: 3827 3827
Size: 6475
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SfcI C'TryA_G
Cuts at: 826 1045 2773 3823 4958 5639 5830 826
Size: 219 1728 1050 1135 681 191 1471
Fragments arranged by size:
1728 1471 1135 1050 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2142 2142
Size: 6475
SfoI GGC'GCC
Cuts at: 2374 2492 3105 2374
Size: 118 613 5744
Fragments arranged by size:
5744 613 118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 3100 1700
Size: 1400 5075
SmaI CCC'GGG
Cuts at: 1872 2487 2909 3802 1872
Size: 615 422 893 4545
Fragments arranged by size:
4545 893 615 422
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4579 5480 5742 820
Size: 197 926 2636 901 262 1553
Fragments arranged by size:
2636 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 3833 1132
Size: 2701 3774
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6475
StyI C'CwwG_G
Cuts at: 513 1065 2004 2200 3499 3509 3914 513
Size: 552 939 196 1299 10 405 3074
Fragments arranged by size:
3074 1299 939 552 405 196 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2410 4502 4519 4672 4857 5302 325
Size: 1255 830 2092 17 153 185 445 1498
Fragments arranged by size:
2092 1498 1255 830 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4721 6433 96
Size: 274 80 33 51 1443 2744 1712 138
Fragments arranged by size:
2744 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6435 6467 6435
Size: 32 6443
Tth111I GACn'n_nGTC
Cuts at: 1316 3482 3610 1316
Size: 2166 128 4181
Fragments arranged by size:
4181 2166 128
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6475
XmaI C'CCGG_G
Cuts at: 1870 2485 2907 3800 1870
Size: 615 422 893 4545
Fragments arranged by size:
4545 893 615 422
XmnI GAAnn'nnTTC
Cuts at: 4604 4604
Size: 6475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI
SapI SexAI SrfI SwaI XcmI