Restriction map for
pFN21AE5063
(Circular) (Six-base) MAPSORT of: pFN21AE5063.seq Check: 2416 from: 1 to: 6256
pFN21AE5063 6256 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2010 3599 1937
Size: 73 1589 4594
Fragments arranged by size:
4594 1589 73
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3330 4319 5703 5971 1632
Size: 1698 989 1384 268 1917
Fragments arranged by size:
1917 1698 1384 989 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 2514 2598 5087 2514
Size: 84 2489 3683
Fragments arranged by size:
3683 2489 84
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 3999 5571 3999
Size: 1572 4684
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 2125 2264 2376 2628 2712 2964 3565 3791
Size: 632 139 112 252 84 252 601 226
Cuts at: 3791 3943 6052 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 632 601 252 252 226 152
139 112 84
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 3581 1870
Size: 73 1638 4545
Fragments arranged by size:
4545 1638 73
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3577 5032 618
Size: 325 217 627 1790 1455 1842
Fragments arranged by size:
1842 1790 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3575 1391
Size: 2184 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5285
BbvCI CC'TCA_GC
Cuts at: 3109 3109
Size: 6256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BfrBI ATG'CAT
Cuts at: 2162 2162
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 4317 5473 1790
Size: 2527 1156 2573
Fragments arranged by size:
2573 2527 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2015 2814 3987
Size: 416 53 83 399 24 598 799 1173
Cuts at: 3987 4945 442
Size: 958 1753
Fragments arranged by size:
1753 1173 958 799 598 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2490 2574 2910 3078 3414 3498 4916 1239
Size: 1251 84 336 168 336 84 1418 2579
Fragments arranged by size:
2579 1418 1251 336 336 168 84 84
Bpu10I CC'TnA_GC
Cuts at: 3109 3109
Size: 6256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4381 5246 5544 4381
Size: 865 298 5093
Fragments arranged by size:
5093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3377 4047 493
Size: 711 2173 670 2702
Fragments arranged by size:
2702 2173 711 670
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4063 4445 5080 275
Size: 53 83 186 936 2530 382 635 1451
Fragments arranged by size:
2530 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2514 2598 4689 5087 5361 5508 5919 2514
Size: 84 2091 398 274 147 411 2851
Fragments arranged by size:
2851 2091 411 398 274 147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6031
Fragments arranged by size:
6031 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6256
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3357 1163
Size: 573 1621 4062
Fragments arranged by size:
4062 1621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 4317 4402 5473 2064
Size: 2253 85 1071 2847
Fragments arranged by size:
2847 2253 1071 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 3575 4317 4402 5473 1391
Size: 399 274 1511 742 85 1071 2174
Fragments arranged by size:
2174 1511 1071 742 399 274 85
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3611 844
Size: 2767 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4143 1394
Size: 429 2320 3507
Fragments arranged by size:
3507 2320 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3349 4751 4925 65
Size: 1183 2101 1402 174 1396
Fragments arranged by size:
2101 1402 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2514 2598 4900 5087 1700
Size: 110 704 84 2302 187 2869
Fragments arranged by size:
2869 2302 704 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 2172 96
Size: 2076 4180
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 3586 3953 4338 4355 5807 5893 5905 6251
Size: 1614 367 385 17 1452 86 12 346
Cuts at: 6251 1972
Size: 1977
Fragments arranged by size:
1977 1614 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2329 513
Size: 552 939 325 4440
Fragments arranged by size:
4440 939 552 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 3772 4566 4586 3772
Size: 794 20 5442
Fragments arranged by size:
5442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3016 3560 3016
Size: 544 5712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 3703 4592
Size: 54 1425 50 275 18 224 1649 889
Cuts at: 4592 6221 8
Size: 1629 43
Fragments arranged by size:
1649 1629 1425 889 275 224 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 4186 1353
Size: 1281 1552 3423
Fragments arranged by size:
3423 1552 1281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2490 2574 2910 3078 3330 3414 3498
Size: 393 858 84 336 168 252 84 84
Cuts at: 3498 4319 4916 5703 5971 1239
Size: 821 597 787 268 1524
Fragments arranged by size:
1524 858 821 787 597 393 336 268
252 168 84 84 84
EcoRI G'AATT_C
Cuts at: 2628 2712 2964 3565 3943 2628
Size: 84 252 601 378 4941
Fragments arranged by size:
4941 601 378 252 84
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 5403 1678
Size: 3725 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4897 4929 4971 5003 1373
Size: 32 135 32 3325 32 42 32 2626
Fragments arranged by size:
3325 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2505 2589 3009 748
Size: 1757 84 420 3995
Fragments arranged by size:
3995 1757 420 84
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2056 10
Size: 54 1425 343 224 4210
Fragments arranged by size:
4210 1425 343 224 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 2821 3501 4214 4573 4732 518
Size: 648 985 670 680 713 359 159 2042
Fragments arranged by size:
2042 985 713 680 670 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3672 4349 5497 5742 1606
Size: 2066 677 1148 245 2120
Fragments arranged by size:
2120 2066 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
NdeI CA'TA_TG
Cuts at: 387 2104 2718 3138 387
Size: 1717 614 420 3505
Fragments arranged by size:
3505 1717 614 420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NsiI A_TGCA'T
Cuts at: 2164 2164
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2166 2350 3614 5159 1132
Size: 97 937 184 1264 1545 2229
Fragments arranged by size:
2229 1545 1264 937 184 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 2187 3826 2187
Size: 1639 4617
PstI C_TGCA'G
Cuts at: 830 3608 830
Size: 2778 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SfcI C'TryA_G
Cuts at: 826 1045 3604 4739 5420 5611 826
Size: 219 2559 1135 681 191 1471
Fragments arranged by size:
2559 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4360 5261 5523 820
Size: 197 926 2417 901 262 1553
Fragments arranged by size:
2417 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 2350 3614 1132
Size: 1218 1264 3774
Fragments arranged by size:
3774 1264 1218
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 2675 3095 1517
Size: 1158 420 4678
Fragments arranged by size:
4678 1158 420
StyI C'CwwG_G
Cuts at: 513 1065 2004 3695 513
Size: 552 939 1691 3074
Fragments arranged by size:
3074 1691 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4283 4300 4453 4638 5083 325
Size: 1255 2703 17 153 185 445 1498
Fragments arranged by size:
2703 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 4502 6214
Size: 274 80 33 51 1443 195 2330 1712
Cuts at: 6214 96
Size: 138
Fragments arranged by size:
2330 1712 1443 274 195 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6216 6248 6216
Size: 32 6224
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 2312 4385 2312
Size: 2073 4183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI