Restriction map for
pFN21AE5061
(Circular) (Six-base) MAPSORT of: pFN21AE5061.seq Check: 1618 from: 1 to: 5878
pFN21AE5061 5878 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 2736 3941 5325 5593 1632
Size: 1085 19 1205 1384 268 1917
Fragments arranged by size:
1917 1384 1205 1085 268 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 2034 4777 5754 1203
Size: 831 2743 977 1327
Fragments arranged by size:
2743 1327 977 831
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 3621 5193 3621
Size: 1572 4306
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2159 2336 2779 3187 3413 3565 5674 1493
Size: 666 177 443 408 226 152 2109 1697
Fragments arranged by size:
2109 1697 666 443 408 226 177 152
AseI AT'TA_AT
Cuts at: 160 1032 2379 4432 160
Size: 872 1347 2053 1606
Fragments arranged by size:
2053 1606 1347 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 3203 1870
Size: 73 1260 4545
Fragments arranged by size:
4545 1260 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3199 4654 618
Size: 325 217 627 1412 1455 1842
Fragments arranged by size:
1842 1455 1412 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3197 1391
Size: 1806 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2705 928
Size: 971 806 4101
Fragments arranged by size:
4101 971 806
BbvCI CC'TCA_GC
Cuts at: 2392 2392
Size: 5878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3001 3770 4980 3001
Size: 769 1210 3899
Fragments arranged by size:
3899 1210 769
BclI T'GATC_A
Cuts at: 1548 2040 2601 2821 1548
Size: 492 561 220 4605
Fragments arranged by size:
4605 561 492 220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2547 5873 2547
Size: 3326 2552
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 3939 5095 1790
Size: 2149 1156 2573
Fragments arranged by size:
2573 2149 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4567 442
Size: 416 53 83 399 24 2192 958 1753
Fragments arranged by size:
2192 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 2536 2849 4538 1239
Size: 1193 104 313 1689 2579
Fragments arranged by size:
2579 1689 1193 313 104
Bpu10I CC'TnA_GC
Cuts at: 2392 2392
Size: 5878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2403 4003 4868 5166 2213
Size: 190 1600 865 298 2925
Fragments arranged by size:
2925 1600 865 298 190
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2297 2717 2849 3050 4541 882
Size: 617 798 420 132 201 1491 2219
Fragments arranged by size:
2219 1491 798 617 420 201 132
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 2747 5872 2747
Size: 3125 2753
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2934 4311 4709 4983 5130 5541 2934
Size: 1377 398 274 147 411 3271
Fragments arranged by size:
3271 1377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 2695 2725 1178
Size: 30 165 30 762 30 500 30 4331
Fragments arranged by size:
4331 762 500 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2969 1186
Size: 1783 4095
BseYI C'CCAG_C
Cuts at: 2768 5081 5577 2768
Size: 2313 496 3069
Fragments arranged by size:
3069 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3939 4024 5095 3939
Size: 85 1071 4722
Fragments arranged by size:
4722 1071 85
BsmI GAATG_Cn'
Cuts at: 2014 2107 3389 3482 2014
Size: 93 1282 93 4410
Fragments arranged by size:
4410 1282 93 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3197 3939 4024 5095 1391
Size: 399 1407 742 85 1071 2174
Fragments arranged by size:
2174 1407 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2934 5541 2934
Size: 2607 3271
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 3233 844
Size: 2089 300 3489
Fragments arranged by size:
3489 2089 300
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2173 4373 4547 65
Size: 1183 925 2200 174 1396
Fragments arranged by size:
2200 1396 1183 925 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2734
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2547 3208 3575 3960 3977 5429 5515 5527
Size: 575 661 367 385 17 1452 86 12
Cuts at: 5527 5873 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 661 575 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 2604 3394 4188 4208 2466
Size: 138 790 794 20 4136
Fragments arranged by size:
4136 794 790 138 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3325 4214 5843
Size: 54 1425 50 275 18 1495 889 1629
Cuts at: 5843 8
Size: 43
Fragments arranged by size:
1629 1495 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 3107 3808 1353
Size: 1298 456 701 3423
Fragments arranged by size:
3423 1298 701 456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 2536 2717 2736 2849 3941 4538
Size: 393 800 104 181 19 113 1092 597
Cuts at: 4538 5325 5593 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1092 800 787 597 393 268 181
113 104 19
EcoRI G'AATT_C
Cuts at: 2336 3187 3565 2336
Size: 851 378 4649
Fragments arranged by size:
4649 851 378
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 5025 1678
Size: 3347 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2496 2528 4519
Size: 32 135 32 593 32 299 32 1991
Cuts at: 4519 4551 4593 4625 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1991 593 299 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2052 2764 3222 3468 669
Size: 988 281 114 712 458 246 3079
Fragments arranged by size:
3079 988 712 458 281 246 114
HindIII A'AGCT_T
Cuts at: 748 2621 2690 748
Size: 1873 69 3936
Fragments arranged by size:
3936 1873 69
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2000 4992 5176 1305
Size: 695 2992 184 2007
Fragments arranged by size:
2992 2007 695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4056
Fragments arranged by size:
4056 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2682 3173 3836 4195 4354 518
Size: 648 1516 491 663 359 159 2042
Fragments arranged by size:
2042 1516 663 648 491 359 159
MspA1I CmG'CkG
Cuts at: 1606 2852 3294 3971 5119 5364 1606
Size: 1246 442 677 1148 245 2120
Fragments arranged by size:
2120 1246 1148 677 442 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5326
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 3236 4781 1132
Size: 97 2007 1545 2229
Fragments arranged by size:
2229 2007 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2331 2331
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2705 1613
Size: 1092 4786
PsiI TTA'TAA
Cuts at: 2510 3084 3448 2510
Size: 574 364 4940
Fragments arranged by size:
4940 574 364
PstI C_TGCA'G
Cuts at: 830 3230 830
Size: 2400 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SapI GCTCTTCn'nnn_
Cuts at: 2651 2651
Size: 5878
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 3226 4361 5042 5233 826
Size: 219 2181 1135 681 191 1471
Fragments arranged by size:
2181 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 2418 3982 4883 5145 820
Size: 197 926 285 190 1564 901 262 1553
Fragments arranged by size:
1564 1553 926 901 285 262 197 190
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 2377 3802 5
Size: 47 2325 1425 2081
Fragments arranged by size:
2325 2081 1425 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 3317 513
Size: 552 2252 3074
Fragments arranged by size:
3074 2252 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3905 3922 4075 4260 4705 325
Size: 1255 2325 17 153 185 445 1498
Fragments arranged by size:
2325 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4124 5836 96
Size: 274 80 33 51 1443 2147 1712 138
Fragments arranged by size:
2147 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2695 2727 5838 5870 2695
Size: 32 3111 32 2703
Fragments arranged by size:
3111 2703 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 4007 4007
Size: 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI