Restriction map for
pFN21AE5060
(Circular) (Six-base) MAPSORT of: pFN21AE5060.seq Check: 8176 from: 1 to: 5884
pFN21AE5060 5884 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2871 2871
Size: 5884
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 2109 2457 3205 2109
Size: 348 748 4788
Fragments arranged by size:
4788 748 348
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2463 3947 5331 5599 1632
Size: 831 1484 1384 268 1917
Fragments arranged by size:
1917 1484 1384 831 268
AflII C'TTAA_G
Cuts at: 820 1017 2188 2530 2848 820
Size: 197 1171 342 318 3856
Fragments arranged by size:
3856 1171 342 318 197
AflIII A'CryG_T
Cuts at: 1203 2390 4783 5760 1203
Size: 1187 2393 977 1327
Fragments arranged by size:
2393 1327 1187 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2237 1184
Size: 1053 4831
AlwNI CAG_nnn'CTG
Cuts at: 3627 5199 3627
Size: 1572 4312
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 2125 2317 2501 2549 3017 3193 3419 3571
Size: 632 192 184 48 468 176 226 152
Cuts at: 3571 5680 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 632 468 226 192 184 176
152 48
AseI AT'TA_AT
Cuts at: 160 1032 2241 2402 4438 160
Size: 872 1209 161 2036 1606
Fragments arranged by size:
2036 1606 1209 872 161
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2307 3209 1870
Size: 73 364 902 4545
Fragments arranged by size:
4545 902 364 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2093 2126 2093
Size: 33 5851
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 2457 3205 4660 618
Size: 325 217 627 322 348 748 1455 1842
Fragments arranged by size:
1842 1455 748 627 348 325 322 217
BanII G_rGCy'C
Cuts at: 1391 3203 1391
Size: 1812 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2947 928
Size: 971 1048 3865
Fragments arranged by size:
3865 1048 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2660 3060 3776 4986 2660
Size: 400 716 1210 3558
Fragments arranged by size:
3558 1210 716 400
BclI T'GATC_A
Cuts at: 1548 2740 1548
Size: 1192 4692
BfrBI ATG'CAT
Cuts at: 2214 2214
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5879 5879
Size: 5884
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 3945 5101 1790
Size: 2155 1156 2573
Fragments arranged by size:
2573 2155 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3615 4573 442
Size: 416 53 83 399 24 2198 958 1753
Fragments arranged by size:
2198 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2379 2377
Size: 2 5882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2670 4544 1239
Size: 1431 1874 2579
Fragments arranged by size:
2579 1874 1431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4009 4874 5172 4009
Size: 865 298 4721
Fragments arranged by size:
4721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4547 882
Size: 617 3048 2219
Fragments arranged by size:
3048 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3675 493
Size: 711 2471 2702
Fragments arranged by size:
2702 2471 711
BsaBI GATnn'nnATC
Cuts at: 2480 2541 2684 5878 2480
Size: 61 143 3194 2486
Fragments arranged by size:
3194 2486 143 61
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3691 4073 4708 275
Size: 53 83 186 936 2158 382 635 1451
Fragments arranged by size:
2158 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2691 4317 4715 4989 5136 5547 2691
Size: 1626 398 274 147 411 3028
Fragments arranged by size:
3028 1626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5659
Fragments arranged by size:
5659 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5884
BseYI C'CCAG_C
Cuts at: 2885 5087 5583 2885
Size: 2202 496 3186
Fragments arranged by size:
3186 2202 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2047 1163
Size: 573 311 5000
Fragments arranged by size:
5000 573 311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3945 4030 5101 3945
Size: 85 1071 4728
Fragments arranged by size:
4728 1071 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
BsmBI CGTCTCn'nnnn_
Cuts at: 2063 2063
Size: 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3203 3945 4030 5101 1391
Size: 399 1413 742 85 1071 2174
Fragments arranged by size:
2174 1413 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2691 5547 2691
Size: 2856 3028
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2871 3159 3239 844
Size: 2027 288 80 3489
Fragments arranged by size:
3489 2027 288 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3099 4379 4553 65
Size: 1183 1851 1280 174 1396
Fragments arranged by size:
1851 1396 1280 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4528 4715 1700
Size: 110 2718 187 2869
Fragments arranged by size:
2869 2718 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstYI r'GATC_y
Cuts at: 1972 2311 3214 3581 3966 3983 5435 5521 5533
Size: 339 903 367 385 17 1452 86 12
Cuts at: 5533 5879 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 903 385 367 346 339 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4393
Fragments arranged by size:
4393 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5145
Fragments arranged by size:
5145 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3144 3400 4194 4214 3130
Size: 14 256 794 20 4800
Fragments arranged by size:
4800 794 256 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 2806 3188 2806
Size: 382 5502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 3331 4220
Size: 54 1425 50 275 18 600 901 889
Cuts at: 4220 5849 8
Size: 1629 43
Fragments arranged by size:
1629 1425 901 889 600 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3075 3814 1353
Size: 1722 739 3423
Fragments arranged by size:
3423 1722 739
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2887 4471 4845 4991 387
Size: 1064 138 1298 1584 374 146 1280
Fragments arranged by size:
1584 1298 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2670 3947 4544 5331 5599 1239
Size: 393 831 207 1277 597 787 268 1524
Fragments arranged by size:
1524 1277 831 787 597 393 268 207
EcoO109I rG'GnC_Cy
Cuts at: 2929 2929
Size: 5884
EcoRI G'AATT_C
Cuts at: 2501 3193 3571 2501
Size: 692 378 4814
Fragments arranged by size:
4814 692 378
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 5031 1678
Size: 3353 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4525 4557 4599 4631 1373
Size: 32 135 32 2953 32 42 32 2626
Fragments arranged by size:
2953 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 3228 3474 669
Size: 988 281 103 1187 246 3079
Fragments arranged by size:
3079 1187 988 281 246 103
HindIII A'AGCT_T
Cuts at: 748 2782 2845 748
Size: 2034 63 3787
Fragments arranged by size:
3787 2034 63
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KpnI G_GTAC'C
Cuts at: 2113 2461 3209 2113
Size: 348 748 4788
Fragments arranged by size:
4788 748 348
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 2644 2989 4998 5182 1305
Size: 904 435 345 2009 184 2007
Fragments arranged by size:
2009 2007 904 435 345 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2432 10
Size: 54 1425 343 600 3462
Fragments arranged by size:
3462 1425 600 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3842 4201 4360 518
Size: 648 2676 359 159 2042
Fragments arranged by size:
2676 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2648 3300 3977 5125 5370 1606
Size: 501 541 652 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 541 501 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4393
Fragments arranged by size:
4393 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NsiI A_TGCA'T
Cuts at: 2216 2216
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2750 3242 4787 1132
Size: 97 1521 492 1545 2229
Fragments arranged by size:
2229 1545 1521 492 97
PasI CC'CwG_GG
Cuts at: 1348 2064 1348
Size: 716 5168
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2648 2839 2945 1108
Size: 1540 191 106 4047
Fragments arranged by size:
4047 1540 191 106
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 2754 3454 2754
Size: 700 5184
PstI C_TGCA'G
Cuts at: 830 3036 3139 3236 830
Size: 2206 103 97 3478
Fragments arranged by size:
3478 2206 103 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2648 2648
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2021 3032 3135 3232 4367 5048 5239
Size: 219 976 1011 103 97 1135 681 191
Cuts at: 5239 826
Size: 1471
Fragments arranged by size:
1471 1135 1011 976 681 219 191 103
97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3211 1872
Size: 1339 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2188 2530 2848 3988 4889 5151
Size: 197 926 245 342 318 1140 901 262
Cuts at: 5151 820
Size: 1553
Fragments arranged by size:
1553 1140 926 901 342 318 262 245
197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5884
StyI C'CwwG_G
Cuts at: 513 1065 2004 2867 3323 513
Size: 552 939 863 456 3074
Fragments arranged by size:
3074 939 863 552 456
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 3928 4081 4266 4711 325
Size: 1255 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5844 5876 5844
Size: 32 5852
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3209 1870
Size: 1339 4545
XmnI GAAnn'nnTTC
Cuts at: 2058 4013 2058
Size: 1955 3929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI