Restriction map for
pFN21AE5057
(Circular) (Six-base) MAPSORT of: pFN21AE5057.seq Check: 9229 from: 1 to: 5281
pFN21AE5057 5281 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3091 278
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 1937
Size: 687 4594
Acc65I G'GTAC_C
Cuts at: 2602 2602
Size: 5281
AclI AA'CG_TT
Cuts at: 1563 3408 3781 1563
Size: 1845 373 3063
Fragments arranged by size:
3063 1845 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 3344 4728 4996 1632
Size: 580 1132 1384 268 1917
Fragments arranged by size:
1917 1384 1132 580 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5084
AflIII A'CryG_T
Cuts at: 1203 2099 4180 5157 1203
Size: 896 2081 977 1327
Fragments arranged by size:
2081 1327 977 896
AgeI A'CCGG_T
Cuts at: 4112 4112
Size: 5281
AhdI GACnn_n'nnGTC
Cuts at: 4010 4010
Size: 5281
AleI CACnn'nnGTG
Cuts at: 1166 2102 1166
Size: 936 4345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5281
AlwNI CAG_nnn'CTG
Cuts at: 3024 4596 3024
Size: 1572 3709
ApaLI G'TGCA_C
Cuts at: 3338 4494 3338
Size: 1156 4125
ApoI r'AATT_y
Cuts at: 1493 2504 2590 2816 2968 5077 1493
Size: 1011 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 1011 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 3835 160
Size: 872 2803 1606
Fragments arranged by size:
2803 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 2606 1870
Size: 73 312 351 4545
Fragments arranged by size:
4545 351 312 73
BamHI G'GATC_C
Cuts at: 2611 2978 2611
Size: 367 4914
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 2602 4057 618
Size: 325 217 627 507 308 1455 1842
Fragments arranged by size:
1842 1455 627 507 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2184 2436 2600 1391
Size: 793 252 164 4072
Fragments arranged by size:
4072 793 252 164
BbeI G_GCGC'C
Cuts at: 2298 2298
Size: 5281
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4310
BbvCI CC'TCA_GC
Cuts at: 2163 2163
Size: 5281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2492 2526 3472 3506 1903
Size: 34 555 34 946 34 3678
Fragments arranged by size:
3678 946 555 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3173 4383 3173
Size: 1210 4071
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 3892 136
Size: 107 122 71 631 605 637 1583 1525
Fragments arranged by size:
1583 1525 637 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5276 5276
Size: 5281
BlpI GC'TnA_GC
Cuts at: 2698 2698
Size: 5281
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2256 3342 4498 1790
Size: 361 105 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 361 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2341 3012 3970
Size: 416 53 83 399 24 924 671 958
Cuts at: 3970 442
Size: 1753
Fragments arranged by size:
1753 958 924 671 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 3941 1239
Size: 1109 1593 2579
Fragments arranged by size:
2579 1593 1109
Bpu10I CC'TnA_GC
Cuts at: 2163 2163
Size: 5281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3406 4271 4569 3406
Size: 865 298 4118
Fragments arranged by size:
4118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2137 2226 3944 882
Size: 617 638 89 1718 2219
Fragments arranged by size:
2219 1718 638 617 89
BsaAI yAC'GTr
Cuts at: 493 1204 2100 3072 493
Size: 711 896 972 2702
Fragments arranged by size:
2702 972 896 711
BsaBI GATnn'nnATC
Cuts at: 5275 5275
Size: 5281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2295 3088 3470 4105
Size: 53 83 186 936 762 793 382 635
Cuts at: 4105 275
Size: 1451
Fragments arranged by size:
1451 936 793 762 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2082 3714 4112 4386 4533 4944 2082
Size: 1632 398 274 147 411 2419
Fragments arranged by size:
2419 1632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5056
Fragments arranged by size:
5056 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2413 2444 2513 1186
Size: 1204 23 31 69 3954
Fragments arranged by size:
3954 1204 69 31 23
BseYI C'CCAG_C
Cuts at: 4484 4980 4484
Size: 496 4785
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 2179 1163
Size: 573 306 137 4265
Fragments arranged by size:
4265 573 306 137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 3492 4520 1540
Size: 275 186 730 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 275 186
BsiHKAI G_wGCw'C
Cuts at: 3342 3427 4498 3342
Size: 85 1071 4125
Fragments arranged by size:
4125 1071 85
BsmI GAATG_Cn'
Cuts at: 2792 2885 2792
Size: 93 5188
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2184 2256 2436 2600 3342 3427
Size: 399 361 33 72 180 164 742 85
Cuts at: 3427 4498 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 180 164 85
72 33
BspEI T'CCGG_A
Cuts at: 2082 4944 2082
Size: 2862 2419
BspHI T'CATG_A
Cuts at: 3170 4900 3170
Size: 1730 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 844
Size: 1792 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3168 1394
Size: 429 1345 3507
Fragments arranged by size:
3507 1345 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3776 3950 65
Size: 1183 2528 174 1396
Fragments arranged by size:
2528 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 3925 4112 1700
Size: 110 248 1867 187 2869
Fragments arranged by size:
2869 1867 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5281
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5281
BssSI C'ACGA_G
Cuts at: 3341 4353 3341
Size: 1012 4269
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5281
BstBI TT'CG_AA
Cuts at: 4085 4085
Size: 5281
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5281
BstYI r'GATC_y
Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276
Size: 639 367 385 17 1452 86 12 346
Cuts at: 5276 1972
Size: 1977
Fragments arranged by size:
1977 1452 639 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2456 2456
Size: 5281
BtgI C'CryG_G
Cuts at: 513 1065 2265 2304 513
Size: 552 1200 39 3490
Fragments arranged by size:
3490 1200 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4542
Fragments arranged by size:
4542 683 56
BtsI GCAGTG_nn'
Cuts at: 2508 2797 3591 3611 2508
Size: 289 794 20 4178
Fragments arranged by size:
4178 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5246
DraI TTT'AAA
Cuts at: 2585 2585
Size: 5281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4288 809
Size: 507 2972 1802
Fragments arranged by size:
2972 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2301 2310
Size: 54 1425 50 275 18 432 39 9
Cuts at: 2310 2728 3617 5246 8
Size: 418 889 1629 43
Fragments arranged by size:
1629 1425 889 432 418 275 54 50
43 39 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2728 1537
Size: 275 916 4090
Fragments arranged by size:
4090 916 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2180 2531 3211 1353
Size: 827 351 680 3423
Fragments arranged by size:
3423 827 680 351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3868 4242 4388 387
Size: 1064 138 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2348 3344 3941 4728 4996 1239
Size: 393 580 136 996 597 787 268 1524
Fragments arranged by size:
1524 996 787 597 580 393 268 136
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2240 2162
Size: 78 5203
EcoRI G'AATT_C
Cuts at: 2504 2590 2968 2504
Size: 86 378 4817
Fragments arranged by size:
4817 378 86
FspI TGC'GCA
Cuts at: 3787 3787
Size: 5281
HaeII r_GCGC'y
Cuts at: 1678 2298 4428 1678
Size: 620 2130 2531
Fragments arranged by size:
2531 2130 620
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 3922 3954 3996
Size: 32 135 32 779 32 1539 32 42
Cuts at: 3996 4028 1373
Size: 32 2626
Fragments arranged by size:
2626 1539 779 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 2871 669
Size: 988 281 687 246 3079
Fragments arranged by size:
3079 988 687 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5281
HpaI GTT'AAC
Cuts at: 2871 2871
Size: 5281
KasI G'GCGC_C
Cuts at: 2294 2294
Size: 5281
KpnI G_GTAC'C
Cuts at: 2606 2606
Size: 5281
MfeI C'AATT_G
Cuts at: 2880 2880
Size: 5281
MluI A'CGCG_T
Cuts at: 5157 5157
Size: 5281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4395 4579 1305
Size: 3090 184 2007
Fragments arranged by size:
3090 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3459
Fragments arranged by size:
3459 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2489 3239 3598 3757 518
Size: 648 936 387 750 359 159 2042
Fragments arranged by size:
2042 936 750 648 387 359 159
MspA1I CmG'CkG
Cuts at: 1606 2544 2697 3374 4522 4767 1606
Size: 938 153 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 245 153
NarI GG'CG_CC
Cuts at: 2295 2295
Size: 5281
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4729
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5281
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5281
NotI GC'GGCC_GC
Cuts at: 2728 2728
Size: 5281
NspI r_CATG'y
Cuts at: 1132 1229 2639 4184 1132
Size: 97 1410 1545 2229
Fragments arranged by size:
2229 1545 1410 97
PasI CC'CwG_GG
Cuts at: 1348 2574 1348
Size: 1226 4055
PciI A'CATG_T
Cuts at: 4180 4180
Size: 5281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5281
PmeI GTTT'AAAC
Cuts at: 2585 2585
Size: 5281
PmlI CAC'GTG
Cuts at: 2100 2100
Size: 5281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4863 4895 3350
Size: 32 1481 32 3736
Fragments arranged by size:
3736 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2012 1613
Size: 399 4882
PsiI TTA'TAA
Cuts at: 2851 2851
Size: 5281
PstI C_TGCA'G
Cuts at: 830 2633 830
Size: 1803 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5281
SalI G'TCGA_C
Cuts at: 1936 2623 1936
Size: 687 4594
SbfI CC_TGCA'GG
Cuts at: 2633 2633
Size: 5281
ScaI AGT'ACT
Cuts at: 3529 5241 3529
Size: 1712 3569
SfcI C'TryA_G
Cuts at: 826 1045 2629 3764 4445 4636 826
Size: 219 1584 1135 681 191 1471
Fragments arranged by size:
1584 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2309 2309
Size: 5281
SfoI GGC'GCC
Cuts at: 2296 2296
Size: 5281
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5281
SmaI CCC'GGG
Cuts at: 1872 2257 2608 1872
Size: 385 351 4545
Fragments arranged by size:
4545 385 351
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3385 4286 4548 820
Size: 197 926 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3072 493
Size: 2579 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5281
SphI G_CATG'C
Cuts at: 1132 2639 1132
Size: 1507 3774
SrfI GCCC'GGGC
Cuts at: 2257 2257
Size: 5281
SspI AAT'ATT
Cuts at: 5 52 3205 5
Size: 47 3153 2081
Fragments arranged by size:
3153 2081 47
StuI AGG'CCT
Cuts at: 1517 2035 1517
Size: 518 4763
StyI C'CwwG_G
Cuts at: 513 1065 2298 2720 513
Size: 552 1233 422 3074
Fragments arranged by size:
3074 1233 552 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2119 2582 3308 3325 3478 3663 4108
Size: 1255 539 463 726 17 153 185 445
Cuts at: 4108 325
Size: 1498
Fragments arranged by size:
1498 1255 726 539 463 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3527 5239 96
Size: 274 80 33 51 1443 1550 1712 138
Fragments arranged by size:
1712 1550 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5241 5273 5241
Size: 32 5249
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5281
XbaI T'CTAG_A
Cuts at: 2617 2617
Size: 5281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2401 2323
Size: 78 5203
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5281
XmaI C'CCGG_G
Cuts at: 1870 2255 2606 1870
Size: 385 351 4545
Fragments arranged by size:
4545 385 351
XmnI GAAnn'nnTTC
Cuts at: 3410 3410
Size: 5281
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3089 276
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SwaI