Restriction map for
pFN21AE5055
(Circular) (Six-base) MAPSORT of: pFN21AE5055.seq Check: 9871 from: 1 to: 5296
pFN21AE5055 5296 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3106 278
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 2639 1937
Size: 487 215 4594
Fragments arranged by size:
4594 487 215
Acc65I G'GTAC_C
Cuts at: 2617 2617
Size: 5296
AclI AA'CG_TT
Cuts at: 1563 3423 3796 1563
Size: 1860 373 3063
Fragments arranged by size:
3063 1860 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3359 4743 5011 1632
Size: 1727 1384 268 1917
Fragments arranged by size:
1917 1727 1384 268
AfeI AGC'GCT
Cuts at: 2012 2012
Size: 5296
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5099
AflIII A'CryG_T
Cuts at: 1203 2361 4195 5172 1203
Size: 1158 1834 977 1327
Fragments arranged by size:
1834 1327 1158 977
AgeI A'CCGG_T
Cuts at: 4127 4127
Size: 5296
AhdI GACnn_n'nnGTC
Cuts at: 2524 4025 2524
Size: 1501 3795
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 2419 1184
Size: 885 350 4061
Fragments arranged by size:
4061 885 350
AlwNI CAG_nnn'CTG
Cuts at: 3039 4611 3039
Size: 1572 3724
ApaI G_GGCC'C
Cuts at: 2197 2271 2197
Size: 74 5222
ApaLI G'TGCA_C
Cuts at: 2178 2561 3353 4509 2178
Size: 383 792 1156 2965
Fragments arranged by size:
2965 1156 792 383
ApoI r'AATT_y
Cuts at: 1493 2605 2831 2983 5092 1493
Size: 1112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3850 160
Size: 872 2818 1606
Fragments arranged by size:
2818 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
AvaI C'yCGr_G
Cuts at: 1870 1943 2432 2621 1870
Size: 73 489 189 4545
Fragments arranged by size:
4545 489 189 73
BamHI G'GATC_C
Cuts at: 2626 2993 2626
Size: 367 4929
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2399 2460 2617 4072 618
Size: 325 217 627 612 61 157 1455 1842
Fragments arranged by size:
1842 1455 627 612 325 217 157 61
BanII G_rGCy'C
Cuts at: 1391 2197 2271 2615 1391
Size: 806 74 344 4072
Fragments arranged by size:
4072 806 344 74
BbeI G_GCGC'C
Cuts at: 2403 2464 2403
Size: 61 5235
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2194 2228 3487 3521 1903
Size: 34 50 34 173 34 1259 34 3678
Fragments arranged by size:
3678 1259 173 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3188 4398 3188
Size: 1210 4086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 2275 2314
Size: 107 122 71 631 605 493 110 39
Cuts at: 2314 2408 3907 136
Size: 94 1499 1525
Fragments arranged by size:
1525 1499 631 605 493 122 110 107
94 71 39
BglII A'GATC_T
Cuts at: 5291 5291
Size: 5296
BlpI GC'TnA_GC
Cuts at: 2713 2713
Size: 5296
Bme1580I G_kGCm'C
Cuts at: 1790 2182 2197 2271 2565 3357 4513 1790
Size: 392 15 74 294 792 1156 2573
Fragments arranged by size:
2573 1156 792 392 294 74 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5296
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 3027 3985
Size: 416 53 83 399 24 747 863 958
Cuts at: 3985 442
Size: 1753
Fragments arranged by size:
1753 958 863 747 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 3956 1239
Size: 1109 1608 2579
Fragments arranged by size:
2579 1608 1109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3421 4286 4584 3421
Size: 865 298 4133
Fragments arranged by size:
4133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3959 882
Size: 617 2460 2219
Fragments arranged by size:
2460 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3087 493
Size: 711 1883 2702
Fragments arranged by size:
2702 1883 711
BsaBI GATnn'nnATC
Cuts at: 5290 5290
Size: 5296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2225 2400 2461
Size: 53 83 186 936 615 77 175 61
Cuts at: 2461 3103 3485 4120 275
Size: 642 382 635 1451
Fragments arranged by size:
1451 936 642 635 615 382 186 175
83 77 61 53
BsaWI w'CCGG_w
Cuts at: 3729 4127 4401 4548 4959 3729
Size: 398 274 147 411 4066
Fragments arranged by size:
4066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5071
Fragments arranged by size:
5071 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5296
BseYI C'CCAG_C
Cuts at: 2234 2347 4499 4995 2234
Size: 113 2152 496 2535
Fragments arranged by size:
2535 2152 496 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 1163
Size: 573 372 4351
Fragments arranged by size:
4351 573 372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2746 3507 4535 1540
Size: 275 186 745 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2182 2565 3357 3442 4513 2043
Size: 139 383 792 85 1071 2826
Fragments arranged by size:
2826 1071 792 383 139 85
BsmI GAATG_Cn'
Cuts at: 2807 2900 2807
Size: 93 5203
BsmBI CGTCTCn'nnnn_
Cuts at: 2366 2366
Size: 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2182 2197 2271 2565 2615 3357
Size: 399 253 139 15 74 294 50 742
Cuts at: 3357 3442 4513 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 294 253 139 85
74 50 15
BspEI T'CCGG_A
Cuts at: 4959 4959
Size: 5296
BspHI T'CATG_A
Cuts at: 3185 4915 3185
Size: 1730 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2116 2651 844
Size: 1272 535 3489
Fragments arranged by size:
3489 1272 535
BsrBI CCG'CTC
Cuts at: 1394 1823 2476 3183 1394
Size: 429 653 707 3507
Fragments arranged by size:
3507 707 653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3791 3965 65
Size: 1183 2543 174 1396
Fragments arranged by size:
2543 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3940 4127 1700
Size: 110 2130 187 2869
Fragments arranged by size:
2869 2130 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5296
BssHII G'CGCG_C
Cuts at: 1925 2281 2283 2417 2482 2484 2576 1925
Size: 356 2 134 65 2 92 4645
Fragments arranged by size:
4645 356 134 92 65 2 2
BssSI C'ACGA_G
Cuts at: 2564 3356 4368 2564
Size: 792 1012 3492
Fragments arranged by size:
3492 1012 792
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2131 794
Size: 1337 3959
BstBI TT'CG_AA
Cuts at: 4100 4100
Size: 5296
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5296
BstYI r'GATC_y
Cuts at: 1972 2626 2993 3378 3395 4847 4933 4945 5291
Size: 654 367 385 17 1452 86 12 346
Cuts at: 5291 1972
Size: 1977
Fragments arranged by size:
1977 1452 654 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4744
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2219 487
Size: 683 56 993 3564
Fragments arranged by size:
3564 993 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3606 3626 2812
Size: 794 20 4482
Fragments arranged by size:
4482 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5261
DraI TTT'AAA
Cuts at: 2600 2600
Size: 5296
DraIII CAC_nnn'GTG
Cuts at: 2560 2560
Size: 5296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4303 809
Size: 507 2987 1802
Fragments arranged by size:
2987 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2743 3632 5261
Size: 54 1425 50 275 18 913 889 1629
Cuts at: 5261 8
Size: 43
Fragments arranged by size:
1629 1425 913 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2743 1537
Size: 275 931 4090
Fragments arranged by size:
4090 931 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3226 1353
Size: 1873 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3883 4257 4403 387
Size: 1064 138 2294 374 146 1280
Fragments arranged by size:
2294 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 3359 3956 4743 5011 1239
Size: 393 716 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 716 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2080 2193 2194 2267 2268 2080
Size: 113 1 73 1 5108
Fragments arranged by size:
5108 113 73 1 1
EcoRI G'AATT_C
Cuts at: 2605 2983 2605
Size: 378 4918
FspI TGC'GCA
Cuts at: 2395 3802 2395
Size: 1407 3889
HaeII r_GCGC'y
Cuts at: 1678 2014 2403 2464 4443 1678
Size: 336 389 61 1979 2531
Fragments arranged by size:
2531 1979 389 336 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 3937 3969 4011
Size: 32 135 32 938 32 1395 32 42
Cuts at: 4011 4043 1373
Size: 32 2626
Fragments arranged by size:
2626 1395 938 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2640 2886 669
Size: 988 281 702 246 3079
Fragments arranged by size:
3079 988 702 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5296
HpaI GTT'AAC
Cuts at: 2886 2886
Size: 5296
KasI G'GCGC_C
Cuts at: 2399 2460 2399
Size: 61 5235
KpnI G_GTAC'C
Cuts at: 2621 2621
Size: 5296
MfeI C'AATT_G
Cuts at: 2895 2895
Size: 5296
MluI A'CGCG_T
Cuts at: 2361 5172 2361
Size: 2811 2485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 4410 4594 1305
Size: 1086 2019 184 2007
Fragments arranged by size:
2019 2007 1086 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3474
Fragments arranged by size:
3474 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 3254 3613 3772 518
Size: 648 900 1188 359 159 2042
Fragments arranged by size:
2042 1188 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2455 2712 3389 4537 4782 1606
Size: 849 257 677 1148 245 2120
Fragments arranged by size:
2120 1148 849 677 257 245
NarI GG'CG_CC
Cuts at: 2400 2461 2400
Size: 61 5235
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4744
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5296
NotI GC'GGCC_GC
Cuts at: 2743 2743
Size: 5296
NspI r_CATG'y
Cuts at: 1132 1229 2654 4199 1132
Size: 97 1425 1545 2229
Fragments arranged by size:
2229 1545 1425 97
PasI CC'CwG_GG
Cuts at: 1348 2229 1348
Size: 881 4415
PciI A'CATG_T
Cuts at: 4195 4195
Size: 5296
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5296
PfoI T'CCnGG_A
Cuts at: 2532 2532
Size: 5296
PmeI GTTT'AAAC
Cuts at: 2600 2600
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 4878 4910 3365
Size: 32 1481 32 3751
Fragments arranged by size:
3751 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5296
PsiI TTA'TAA
Cuts at: 2866 2866
Size: 5296
PspOMI G'GGCC_C
Cuts at: 2193 2267 2193
Size: 74 5222
PstI C_TGCA'G
Cuts at: 830 2048 2190 2648 830
Size: 1218 142 458 3478
Fragments arranged by size:
3478 1218 458 142
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5296
SalI G'TCGA_C
Cuts at: 1936 2638 1936
Size: 702 4594
SbfI CC_TGCA'GG
Cuts at: 2190 2648 2190
Size: 458 4838
ScaI AGT'ACT
Cuts at: 3544 5256 3544
Size: 1712 3584
SfcI C'TryA_G
Cuts at: 826 1045 2044 2186 2644 3779 4460 4651 826
Size: 219 999 142 458 1135 681 191 1471
Fragments arranged by size:
1471 1135 999 681 458 219 191 142
SfoI GGC'GCC
Cuts at: 2401 2462 2401
Size: 61 5235
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5296
SmaI CCC'GGG
Cuts at: 1872 2623 1872
Size: 751 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3400 4301 4563 820
Size: 197 926 1457 901 262 1553
Fragments arranged by size:
1553 1457 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3087 493
Size: 2594 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5296
SphI G_CATG'C
Cuts at: 1132 2654 1132
Size: 1522 3774
SspI AAT'ATT
Cuts at: 5 52 3220 5
Size: 47 3168 2081
Fragments arranged by size:
3168 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5296
StyI C'CwwG_G
Cuts at: 513 1065 2028 2735 513
Size: 552 963 707 3074
Fragments arranged by size:
3074 963 707 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3323 3340 3493 3678 4123 325
Size: 1255 1743 17 153 185 445 1498
Fragments arranged by size:
1743 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3542 5254 96
Size: 274 80 33 51 1443 1565 1712 138
Fragments arranged by size:
1712 1565 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5256 5288 5256
Size: 32 5264
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5296
XbaI T'CTAG_A
Cuts at: 2632 2632
Size: 5296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5296
XmaI C'CCGG_G
Cuts at: 1870 2621 1870
Size: 751 4545
XmnI GAAnn'nnTTC
Cuts at: 3425 3425
Size: 5296
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3104 276
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI