Restriction map for
pFN21AE5022
(Circular) (Six-base) MAPSORT of: pFN21AE5022.seq Check: 6417 from: 1 to: 6013
pFN21AE5022 6013 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2444 3823 278
Size: 53 83 186 936 908 1379 2468
Fragments arranged by size:
2468 1379 936 908 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3036 3356 1937
Size: 1099 320 4594
Fragments arranged by size:
4594 1099 320
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2587 4076 5460 5728 1632
Size: 955 1489 1384 268 1917
Fragments arranged by size:
1917 1489 1384 955 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 2392 4912 5889 1203
Size: 1189 2520 977 1327
Fragments arranged by size:
2520 1327 1189 977
AgeI A'CCGG_T
Cuts at: 2122 3079 4844 2122
Size: 957 1765 3291
Fragments arranged by size:
3291 1765 957
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 2246 2700 1166
Size: 1080 454 4479
Fragments arranged by size:
4479 1080 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2532 1184
Size: 1348 4665
AlwNI CAG_nnn'CTG
Cuts at: 2713 3756 5328 2713
Size: 1043 1572 3398
Fragments arranged by size:
3398 1572 1043
ApaI G_GGCC'C
Cuts at: 2023 2091 2023
Size: 68 5945
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 3322 3548 3700 5809 1493
Size: 1829 226 152 2109 1697
Fragments arranged by size:
2109 1829 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2912 3273 3338 1870
Size: 73 147 822 361 65 4545
Fragments arranged by size:
4545 822 361 147 73 65
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2113 2119 2145 2789
Size: 325 217 627 252 74 6 26 644
Cuts at: 2789 3334 4789 618
Size: 545 1455 1842
Fragments arranged by size:
1842 1455 644 627 545 325 252 217
74 26 6
BanII G_rGCy'C
Cuts at: 1391 2023 2091 3332 1391
Size: 632 68 1241 4072
Fragments arranged by size:
4072 1241 632 68
BbeI G_GCGC'C
Cuts at: 2043 2117 2149 2043
Size: 74 32 5907
Fragments arranged by size:
5907 74 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2593 928
Size: 971 694 4348
Fragments arranged by size:
4348 971 694
BbvCI CC'TCA_GC
Cuts at: 2033 2033
Size: 6013
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 2067 2492
Size: 107 122 71 631 605 365 30 425
Cuts at: 2492 2567 2621 2879 4624 136
Size: 75 54 258 1745 1525
Fragments arranged by size:
1745 1525 631 605 425 365 258 122
107 75 71 54 30
BglII A'GATC_T
Cuts at: 6008 6008
Size: 6013
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2091 2178 2483 2707 4074 5230 1790
Size: 233 68 87 305 224 1367 1156 2573
Fragments arranged by size:
2573 1367 1156 305 233 224 87 68
BmgBI CAC'GTC
Cuts at: 1318 2254 2870 1318
Size: 936 616 4461
Fragments arranged by size:
4461 936 616
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3019 3744 4702
Size: 416 53 83 399 24 1602 725 958
Cuts at: 4702 442
Size: 1753
Fragments arranged by size:
1753 1602 958 725 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3270 1056
Size: 2214 3799
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 3294 4673 1239
Size: 1211 844 1379 2579
Fragments arranged by size:
2579 1379 1211 844
Bpu10I CC'TnA_GC
Cuts at: 2033 2225 3093 2033
Size: 192 868 4953
Fragments arranged by size:
4953 868 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3119 4138 5003 5301 3119
Size: 1019 865 298 3831
Fragments arranged by size:
3831 1019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4676 882
Size: 617 3177 2219
Fragments arranged by size:
3177 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 2698 3804 493
Size: 711 1191 303 1106 2702
Fragments arranged by size:
2702 1191 1106 711 303
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2114 2146 2441
Size: 53 83 186 936 507 74 32 295
Cuts at: 2441 2535 3820 4202 4837 275
Size: 94 1285 382 635 1451
Fragments arranged by size:
1451 1285 936 635 507 382 295 186
94 83 74 53 32
BsaWI w'CCGG_w
Cuts at: 2122 3079 4446 4844 5118 5265 5676 2122
Size: 957 1367 398 274 147 411 2459
Fragments arranged by size:
2459 1367 957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3292 3322 1178
Size: 30 165 30 1889 30 3869
Fragments arranged by size:
3869 1889 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2489 1186
Size: 907 396 4710
Fragments arranged by size:
4710 907 396
BseYI C'CCAG_C
Cuts at: 2706 5216 5712 2706
Size: 2510 496 3007
Fragments arranged by size:
3007 2510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2330 1163
Size: 573 411 183 4846
Fragments arranged by size:
4846 573 411 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2155 2487 2797 3463 4224 5252
Size: 275 186 154 332 310 666 761 1028
Cuts at: 5252 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 666 332 310 275 186
154
BsiHKAI G_wGCw'C
Cuts at: 2532 4074 4159 5230 2532
Size: 1542 85 1071 3315
Fragments arranged by size:
3315 1542 1071 85
BsmI GAATG_Cn'
Cuts at: 3524 3617 3524
Size: 93 5920
BsmBI CGTCTCn'nnnn_
Cuts at: 2426 2585 2426
Size: 159 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2091 2178 2483 2532 2707 3332
Size: 399 233 68 87 305 49 175 625
Cuts at: 3332 4074 4159 5230 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 399 305 233 175
87 85 68 49
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 3368 844
Size: 1832 692 3489
Fragments arranged by size:
3489 1832 692
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2334 2514 4508 4682 65
Size: 1183 1086 180 1994 174 1396
Fragments arranged by size:
1994 1396 1183 1086 180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2116 2122 2142 2519 3079 4657 4844
Size: 110 306 6 20 377 560 1578 187
Cuts at: 4844 1700
Size: 2869
Fragments arranged by size:
2869 1578 560 377 306 187 110 20
6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 2214 2216 1925
Size: 289 2 5722
Fragments arranged by size:
5722 289 2
BssSI C'ACGA_G
Cuts at: 2807 4073 5085 2807
Size: 1266 1012 3735
Fragments arranged by size:
3735 1266 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6013
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2248 2407 2011
Size: 237 159 5617
Fragments arranged by size:
5617 237 159
BstYI r'GATC_y
Cuts at: 1972 3343 3710 4095 4112 5564 5650 5662 6008
Size: 1371 367 385 17 1452 86 12 346
Cuts at: 6008 1972
Size: 1977
Fragments arranged by size:
1977 1452 1371 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2149 2499 513
Size: 552 939 132 13 350 4027
Fragments arranged by size:
4027 939 552 350 132 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2085 3038 487
Size: 683 56 859 953 3462
Fragments arranged by size:
3462 953 859 683 56
BtsI GCAGTG_nn'
Cuts at: 2176 2516 3094 3529 4323 4343 2176
Size: 340 578 435 794 20 3846
Fragments arranged by size:
3846 794 578 435 340 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DraIII CAC_nnn'GTG
Cuts at: 2949 2949
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2440 2540 5020 809
Size: 507 1124 100 2480 1802
Fragments arranged by size:
2480 1802 1124 507 100
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2206 2358
Size: 54 1425 50 275 18 183 193 152
Cuts at: 2358 2397 2409 2484 2493 2559 2568 2880 3460
Size: 39 12 75 9 66 9 312 580
Cuts at: 3460 4349 5978 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 580 312 275 193 183
152 75 66 54 50 43 39 18
12 9 9
EagI C'GGCC_G
Cuts at: 1537 1812 2484 3460 1537
Size: 275 672 976 4090
Fragments arranged by size:
4090 976 672 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2285 2318 2426 3014 3943 1353
Size: 717 215 33 108 588 929 3423
Fragments arranged by size:
3423 929 717 588 215 108 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2587 3294 4076 4673 5460 5728
Size: 393 818 137 707 782 597 787 268
Cuts at: 5728 1239
Size: 1524
Fragments arranged by size:
1524 818 787 782 707 597 393 268
137
EcoNI CCTnn'n_nnAGG
Cuts at: 2687 2687
Size: 6013
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 3067 2019
Size: 1 1047 4965
Fragments arranged by size:
4965 1047 1
EcoRI G'AATT_C
Cuts at: 3322 3700 3322
Size: 378 5635
FspI TGC'GCA
Cuts at: 2462 4519 2462
Size: 2057 3956
HaeII r_GCGC'y
Cuts at: 1678 2043 2117 2149 2266 2627 3299 5160 1678
Size: 365 74 32 117 361 672 1861 2531
Fragments arranged by size:
2531 1861 672 365 361 117 74 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3292 3324 4654 4686 4728
Size: 32 135 32 1720 32 1330 32 42
Cuts at: 4728 4760 1373
Size: 32 2626
Fragments arranged by size:
2626 1720 1330 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3037 3357 3603 669
Size: 988 281 1099 320 246 3079
Fragments arranged by size:
3079 1099 988 320 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KasI G'GCGC_C
Cuts at: 2039 2113 2145 2039
Size: 74 32 5907
Fragments arranged by size:
5907 74 32
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5127 5311 1305
Size: 3822 184 2007
Fragments arranged by size:
3822 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2015 2208 2399 2561 2570
Size: 54 1425 343 183 193 191 162 9
Cuts at: 2570 2882 10
Size: 312 3141
Fragments arranged by size:
3141 1425 343 312 193 191 183 162
54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2700 2763 2936 3971 4330 4489
Size: 648 1080 454 63 173 1035 359 159
Cuts at: 4489 518
Size: 2042
Fragments arranged by size:
2042 1080 1035 648 454 359 173 159
63
MspA1I CmG'CkG
Cuts at: 1606 2037 2151 2489 2710 2752 3429 4106 5254
Size: 431 114 338 221 42 677 677 1148
Cuts at: 5254 5499 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 677 431 338 245 221
114 42
NaeI GCC'GGC
Cuts at: 2118 2144 2521 2118
Size: 26 377 5610
Fragments arranged by size:
5610 377 26
NarI GG'CG_CC
Cuts at: 2040 2114 2146 2040
Size: 74 32 5907
Fragments arranged by size:
5907 74 32
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4522
Fragments arranged by size:
4522 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NgoMIV G'CCGG_C
Cuts at: 2116 2142 2519 2116
Size: 26 377 5610
Fragments arranged by size:
5610 377 26
NheI G'CTAG_C
Cuts at: 1052 3266 1052
Size: 2214 3799
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2247 3371 4916 1132
Size: 97 1018 1124 1545 2229
Fragments arranged by size:
2229 1545 1124 1018 97
PasI CC'CwG_GG
Cuts at: 1348 3071 1348
Size: 1723 4290
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 1108
Size: 1659 4354
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PmlI CAC'GTG
Cuts at: 2395 2698 2395
Size: 303 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3067 3067
Size: 6013
PshAI GACnn'nnGTC
Cuts at: 1613 2597 3080 1613
Size: 984 483 4546
Fragments arranged by size:
4546 984 483
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspOMI G'GGCC_C
Cuts at: 2019 2087 2019
Size: 68 5945
PstI C_TGCA'G
Cuts at: 830 2511 2718 3089 3365 830
Size: 1681 207 371 276 3478
Fragments arranged by size:
3478 1681 371 276 207
PvuI CG_AT'CG
Cuts at: 2001 2797 2001
Size: 796 5217
PvuII CAG'CTG
Cuts at: 2710 2710
Size: 6013
SacII CC_GC'GG
Cuts at: 2152 2152
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3035 3355 1936
Size: 1099 320 4594
Fragments arranged by size:
4594 1099 320
SapI GCTCTTCn'nnn_
Cuts at: 2285 2318 2285
Size: 33 5980
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SexAI A'CCwGG_T
Cuts at: 2682 2682
Size: 6013
SfcI C'TryA_G
Cuts at: 826 1045 2507 2714 3085 3361 4496 5177 5368
Size: 219 1462 207 371 276 1135 681 191
Cuts at: 5368 826
Size: 1471
Fragments arranged by size:
1471 1462 1135 681 371 276 219 207
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2492 2567 2492
Size: 75 5938
SfoI GGC'GCC
Cuts at: 2041 2115 2147 2041
Size: 74 32 5907
Fragments arranged by size:
5907 74 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 2122 1700
Size: 422 5591
SmaI CCC'GGG
Cuts at: 1872 2092 3340 1872
Size: 220 1248 4545
Fragments arranged by size:
4545 1248 220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2912 3134 4117 5018 5280 820
Size: 197 926 969 222 983 901 262 1553
Fragments arranged by size:
1553 983 969 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2004 3452 513
Size: 552 939 1448 3074
Fragments arranged by size:
3074 1448 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 2537 4040 4057 4210 4395 4840
Size: 1255 612 345 1503 17 153 185 445
Cuts at: 4840 325
Size: 1498
Fragments arranged by size:
1503 1498 1255 612 445 345 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 4259 5971
Size: 274 80 33 51 1443 765 1517 1712
Cuts at: 5971 96
Size: 138
Fragments arranged by size:
1712 1517 1443 765 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 2912 1943
Size: 969 5044
XmaI C'CCGG_G
Cuts at: 1870 2090 3338 1870
Size: 220 1248 4545
Fragments arranged by size:
4545 1248 220
XmnI GAAnn'nnTTC
Cuts at: 4142 4142
Size: 6013
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2442 3821 276
Size: 53 83 186 936 908 1379 2468
Fragments arranged by size:
2468 1379 936 908 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI
SrfI SwaI XcmI