Restriction map for
pFN21AE5016
(Circular) (Six-base) MAPSORT of: pFN21AE5016.seq Check: 7235 from: 1 to: 5707
pFN21AE5016 5707 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2604 2604
Size: 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3770 5154 5422 1632
Size: 2138 1384 268 1917
Fragments arranged by size:
2138 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 2150 2958 1166
Size: 984 808 3915
Fragments arranged by size:
3915 984 808
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2168 1184
Size: 984 4723
AlwNI CAG_nnn'CTG
Cuts at: 2302 2374 3450 5022 2302
Size: 72 1076 1572 2987
Fragments arranged by size:
2987 1572 1076 72
ApaI G_GGCC'C
Cuts at: 2015 2083 2015
Size: 68 5639
ApaLI G'TGCA_C
Cuts at: 2143 3764 4920 2143
Size: 1621 1156 2930
Fragments arranged by size:
2930 1621 1156
ApoI r'AATT_y
Cuts at: 1493 3016 3242 3394 5503 1493
Size: 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 152
AscI GG'CGCG_CC
Cuts at: 2471 2471
Size: 5707
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2135 2203 2807 2977 3032 1870
Size: 73 164 28 68 604 170 55 4545
Fragments arranged by size:
4545 604 170 164 73 68 55 28
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2270 2407 2658 3028
Size: 325 217 627 333 150 137 251 370
Cuts at: 3028 4483 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 370 333 325 251 217
150 137
BanII G_rGCy'C
Cuts at: 1391 2015 2083 2484 2858 2865 2987 3026 1391
Size: 624 68 401 374 7 122 39 4072
Fragments arranged by size:
4072 624 401 374 122 68 39 7
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4736
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3898 3932 1903
Size: 34 50 34 1877 34 3678
Fragments arranged by size:
3678 1877 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 2720 3124 2720
Size: 404 5303
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2083 2147 2202 3768 4924 1790
Size: 225 68 64 55 1566 1156 2573
Fragments arranged by size:
2573 1566 1156 225 68 64 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 4367 1239
Size: 1259 1869 2579
Fragments arranged by size:
2579 1869 1259
Bpu10I CC'TnA_GC
Cuts at: 2210 2381 2210
Size: 171 5536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2914 4370 882
Size: 617 1415 1456 2219
Fragments arranged by size:
2219 1456 1415 617
BsaAI yAC'GTr
Cuts at: 493 1204 2148 3498 493
Size: 711 944 1350 2702
Fragments arranged by size:
2702 1350 944 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2710 2745 3514
Size: 53 83 186 936 588 589 35 769
Cuts at: 3514 3896 4531 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 769 635 589 588 382 186
83 53 35
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 2563 2851 4910 5406 2563
Size: 288 2059 496 2864
Fragments arranged by size:
2864 2059 496 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 2576 1163
Size: 573 573 267 4294
Fragments arranged by size:
4294 573 573 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2036 3157 3918 4946 1540
Size: 275 186 35 1121 761 1028 2301
Fragments arranged by size:
2301 1121 1028 761 275 186 35
BsiHKAI G_wGCw'C
Cuts at: 2147 2694 3768 3853 4924 2147
Size: 547 1074 85 1071 2930
Fragments arranged by size:
2930 1074 1071 547 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
BsmBI CGTCTCn'nnnn_
Cuts at: 2678 2678
Size: 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2083 2147 2202 2484 2694 2858
Size: 399 225 68 64 55 282 210 164
Cuts at: 2858 2865 2987 3026 3768 3853 4924 1391
Size: 7 122 39 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 225 210 164
122 85 68 64 55 39 7
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2604 3062 844
Size: 1472 288 458 3489
Fragments arranged by size:
3489 1472 458 288
BsrBI CCG'CTC
Cuts at: 1394 1823 2285 2579 2638 2773 3594 1394
Size: 429 462 294 59 135 821 3507
Fragments arranged by size:
3507 821 462 429 294 135 59
BsrDI GCAATG_nn'
Cuts at: 65 1248 2758 4202 4376 65
Size: 1183 1510 1444 174 1396
Fragments arranged by size:
1510 1444 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2456 2463 2655 2812 4351 4538 1700
Size: 110 646 7 192 157 1539 187 2869
Fragments arranged by size:
2869 1539 646 192 187 157 110 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 2224 2226 2289 2361 2431 2433 2471 2496
Size: 299 2 63 72 70 2 38 25
Cuts at: 2496 2625 2763 2879 1925
Size: 129 138 116 4753
Fragments arranged by size:
4753 299 138 129 116 72 70 63
38 25 2 2
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2332 2665 794
Size: 1538 333 3836
Fragments arranged by size:
3836 1538 333
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 2395 2395
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702
Size: 1065 367 385 17 1452 86 12 346
Cuts at: 5702 1972
Size: 1977
Fragments arranged by size:
1977 1452 1065 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2094 2484 2793 2953 2970 513
Size: 552 1029 390 309 160 17 3250
Fragments arranged by size:
3250 1029 552 390 309 160 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4017 4037 3223
Size: 794 20 4893
Fragments arranged by size:
4893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2332 2332
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2091 2166
Size: 54 1425 50 275 18 203 58 75
Cuts at: 2166 2454 2702 2790 3154 4043 5672 8
Size: 288 248 88 364 889 1629 43
Fragments arranged by size:
1629 1425 889 364 288 275 248 203
88 75 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2033 3154 1537
Size: 275 221 1121 4090
Fragments arranged by size:
4090 1121 275 221
EarI CTCTTCn'nnn_
Cuts at: 1353 3637 1353
Size: 2284 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2900 4294 4668 4814 387
Size: 1064 138 1311 1394 374 146 1280
Fragments arranged by size:
1394 1311 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2498 3770 4367 5154 5422 1239
Size: 393 866 1272 597 787 268 1524
Fragments arranged by size:
1524 1272 866 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2011 2012 2112 2353 2785 2011
Size: 1 100 241 432 4933
Fragments arranged by size:
4933 432 241 100 1
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FseI GG_CCGG'CC
Cuts at: 2816 2816
Size: 5707
FspI TGC'GCA
Cuts at: 2441 4213 2441
Size: 1772 3935
HaeII r_GCGC'y
Cuts at: 1678 2124 2302 2388 2492 2852 4854 1678
Size: 446 178 86 104 360 2002 2531
Fragments arranged by size:
2531 2002 446 360 178 104 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 4821 5005 1305
Size: 1461 2055 184 2007
Fragments arranged by size:
2055 2007 1461 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 10
Size: 54 1425 343 336 3549
Fragments arranged by size:
3549 1425 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2958 3665 4024 4183 518
Size: 648 984 808 707 359 159 2042
Fragments arranged by size:
2042 984 808 707 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2172 2371 2395 2428 2486 2506 2665
Size: 432 134 199 24 33 58 20 159
Cuts at: 2665 2972 2993 3123 3800 4948 5193 1606
Size: 307 21 130 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 432 307 245 199 159
134 130 58 33 24 21 20
NaeI GCC'GGC
Cuts at: 2458 2657 2814 2458
Size: 199 157 5351
Fragments arranged by size:
5351 199 157
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5155
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NgoMIV G'CCGG_C
Cuts at: 2456 2655 2812 2456
Size: 199 157 5351
Fragments arranged by size:
5351 199 157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2339 3065 4610 1132
Size: 97 1110 726 1545 2229
Fragments arranged by size:
2229 1545 1110 726 97
PasI CC'CwG_GG
Cuts at: 1348 2116 1348
Size: 768 4939
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PmlI CAC'GTG
Cuts at: 2148 2148
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2112 2785 2112
Size: 673 5034
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PspOMI G'GGCC_C
Cuts at: 2011 2079 2011
Size: 68 5639
PstI C_TGCA'G
Cuts at: 830 2370 2529 3059 830
Size: 1540 159 530 3478
Fragments arranged by size:
3478 1540 530 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2172 2371 2395 2172
Size: 199 24 5484
Fragments arranged by size:
5484 199 24
SacII CC_GC'GG
Cuts at: 2487 2973 2487
Size: 486 5221
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SanDI GG'GwC_CC
Cuts at: 2112 2112
Size: 5707
SbfI CC_TGCA'GG
Cuts at: 2529 3059 2529
Size: 530 5177
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 2294 2366 2525 3055 4190 4871 5062
Size: 219 1249 72 159 530 1135 681 191
Cuts at: 5062 826
Size: 1471
Fragments arranged by size:
1471 1249 1135 681 530 219 191 159
72
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 2205 2979 3034 1872
Size: 333 774 55 4545
Fragments arranged by size:
4545 774 333 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 2163 1517
Size: 646 5061
StyI C'CwwG_G
Cuts at: 513 1065 2066 2609 3146 513
Size: 552 1001 543 537 3074
Fragments arranged by size:
3074 1001 552 543 537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 3751 3904 4089 4534 325
Size: 1255 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3953 5665 96
Size: 274 80 33 51 1443 1976 1712 138
Fragments arranged by size:
1976 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 2581 3043 2581
Size: 462 5245
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 2203 2977 3032 1870
Size: 333 774 55 4545
Fragments arranged by size:
4545 774 333 55
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SapI
SexAI SfiI SrfI SwaI XcmI