Restriction map for
pFN21AE5015
(Circular) (Six-base) MAPSORT of: pFN21AE5015.seq Check: 3612 from: 1 to: 5695
pFN21AE5015 5695 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2656 3758 5142 5410 1632
Size: 1024 1102 1384 268 1917
Fragments arranged by size:
1917 1384 1102 1024 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 2126 4424 2126
Size: 2298 3397
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 2122 2386 3438 5010 2122
Size: 264 1052 1572 2807
Fragments arranged by size:
2807 1572 1052 264
ApaI G_GGCC'C
Cuts at: 2980 2980
Size: 5695
ApaLI G'TGCA_C
Cuts at: 2044 2882 3752 4908 2044
Size: 838 870 1156 2831
Fragments arranged by size:
2831 1156 870 838
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2522 2765 2800 2810 2932 2938
Size: 73 147 432 243 35 10 122 6
Cuts at: 2938 3020 1870
Size: 82 4545
Fragments arranged by size:
4545 432 243 147 122 82 73 35
10 6
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2415 2711 2806 3016 4471
Size: 325 217 627 628 296 95 210 1455
Cuts at: 4471 618
Size: 1842
Fragments arranged by size:
1842 1455 628 627 325 296 217 210
95
BanII G_rGCy'C
Cuts at: 1391 2217 2700 2861 2939 2980 3014 1391
Size: 826 483 161 78 41 34 4072
Fragments arranged by size:
4072 826 483 161 78 41 34
BbeI G_GCGC'C
Cuts at: 2419 2715 2810 2419
Size: 296 95 5304
Fragments arranged by size:
5304 296 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2071 2320 2863 928
Size: 971 172 249 543 3760
Fragments arranged by size:
3760 971 543 249 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2756 2790 3886 3920 1903
Size: 34 50 34 735 34 1096 34 3678
Fragments arranged by size:
3678 1096 735 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2516 2525 4306
Size: 107 122 71 631 605 844 9 1781
Cuts at: 4306 136
Size: 1525
Fragments arranged by size:
1781 1525 844 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 2211 3112 2211
Size: 901 4794
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2886 2980 3756 4912 1790
Size: 258 838 94 776 1156 2573
Fragments arranged by size:
2573 1156 838 776 258 94
BmgBI CAC'GTC
Cuts at: 1318 2460 1318
Size: 1142 4553
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2210 2912 4355 1239
Size: 950 21 702 1443 2579
Fragments arranged by size:
2579 1443 950 702 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4685 4983 3820
Size: 865 298 4532
Fragments arranged by size:
4532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2665 2881 3486 493
Size: 711 1461 216 605 2702
Fragments arranged by size:
2702 1461 711 605 216
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2416 2712 2807 3502
Size: 53 83 186 936 883 296 95 695
Cuts at: 3502 3884 4519 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 883 695 635 382 296 186
95 83 53
BsaWI w'CCGG_w
Cuts at: 2148 4128 4526 4800 4947 5358 2148
Size: 1980 398 274 147 411 2485
Fragments arranged by size:
2485 1980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 2786 4898 5394 2786
Size: 2112 496 3087
Fragments arranged by size:
3087 2112 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2113 2172 2230 3145 3906
Size: 275 186 30 82 59 58 915 761
Cuts at: 3906 4934 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 915 761 275 186 82 59
58 30
BsiHKAI G_wGCw'C
Cuts at: 2048 2886 3756 3841 4912 2048
Size: 838 870 85 1071 2831
Fragments arranged by size:
2831 1071 870 838 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2217 2700 2861 2886 2939 2980
Size: 399 258 169 483 161 25 53 41
Cuts at: 2980 3014 3756 3841 4912 1391
Size: 34 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 483 399 258 169 161
85 53 41 34 25
BspEI T'CCGG_A
Cuts at: 2148 5358 2148
Size: 3210 2485
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 3050 844
Size: 1223 983 3489
Fragments arranged by size:
3489 1223 983
BsrBI CCG'CTC
Cuts at: 1394 1823 2634 2850 3582 1394
Size: 429 811 216 732 3507
Fragments arranged by size:
3507 811 732 429 216
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2225 4339 4526 1700
Size: 110 415 2114 187 2869
Fragments arranged by size:
2869 2114 415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 2023 2770 1925
Size: 98 747 4850
Fragments arranged by size:
4850 747 98
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690
Size: 1053 367 385 17 1452 86 12 346
Cuts at: 5690 1972
Size: 1977
Fragments arranged by size:
1977 1452 1053 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2469 2818 2853 513
Size: 552 1404 349 35 3355
Fragments arranged by size:
3355 1404 552 349 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2624 3211 4005 4025 2624
Size: 587 794 20 4294
Fragments arranged by size:
4294 794 587 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2052 2052
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2464 4702 809
Size: 507 1148 2238 1802
Fragments arranged by size:
2238 1802 1148 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2110 2169
Size: 54 1425 50 275 18 198 82 59
Cuts at: 2169 2227 2293 2430 2487 3142 4031 5660 8
Size: 58 66 137 57 655 889 1629 43
Fragments arranged by size:
1629 1425 889 655 275 198 137 82
66 59 58 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 2110 2169 2227 3142 1537
Size: 275 216 82 59 58 915 4090
Fragments arranged by size:
4090 915 275 216 82 59 58
EarI CTCTTCn'nnn_
Cuts at: 1353 3625 1353
Size: 2272 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 2851 4282 4656 4802 387
Size: 1064 138 528 734 1431 374 146 1280
Fragments arranged by size:
1431 1280 1064 734 528 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2210 2656 2912 3758 4355 5142
Size: 393 557 21 446 256 846 597 787
Cuts at: 5142 5410 1239
Size: 268 1524
Fragments arranged by size:
1524 846 787 597 557 446 393 268
256 21
EcoO109I rG'GnC_Cy
Cuts at: 2976 2976
Size: 5695
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2073 2145 2197 2204 2419 2645 2715 2757
Size: 395 72 52 7 215 226 70 42
Cuts at: 2757 2810 4842 1678
Size: 53 2032 2531
Fragments arranged by size:
2531 2032 395 226 215 72 70 53
52 42 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2112 2144 4336 4368 4410
Size: 32 135 32 540 32 2192 32 42
Cuts at: 4410 4442 1373
Size: 32 2626
Fragments arranged by size:
2626 2192 540 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KasI G'GCGC_C
Cuts at: 2415 2711 2806 2415
Size: 296 95 5304
Fragments arranged by size:
5304 296 95
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3653 4012 4171 518
Size: 648 2487 359 159 2042
Fragments arranged by size:
2487 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 3111 3788 4936 5181 1606
Size: 756 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 756 749 677 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 5695
NarI GG'CG_CC
Cuts at: 2416 2712 2807 2416
Size: 296 95 5304
Fragments arranged by size:
5304 296 95
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 2110 3142 2110
Size: 1032 4663
NspI r_CATG'y
Cuts at: 1132 1229 2338 3053 4598 1132
Size: 97 1109 715 1545 2229
Fragments arranged by size:
2229 1545 1109 715 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 1108
Size: 1278 4417
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PmlI CAC'GTG
Cuts at: 2881 2881
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2369 1613
Size: 756 4939
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2976 2976
Size: 5695
PspXI vC'TCGA_Gb
Cuts at: 2090 2932 2090
Size: 842 4853
PstI C_TGCA'G
Cuts at: 830 2631 2649 3047 830
Size: 1801 18 398 3478
Fragments arranged by size:
3478 1801 398 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SexAI A'CCwGG_T
Cuts at: 2478 2724 2478
Size: 246 5449
SfcI C'TryA_G
Cuts at: 826 1045 2627 2645 3043 4178 4859 5050 826
Size: 219 1582 18 398 1135 681 191 1471
Fragments arranged by size:
1582 1471 1135 681 398 219 191 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2525 2525
Size: 5695
SfoI GGC'GCC
Cuts at: 2417 2713 2808 2417
Size: 296 95 5304
Fragments arranged by size:
5304 296 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 2802 2940 3022 1872
Size: 930 138 82 4545
Fragments arranged by size:
4545 930 138 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2932 3799 4700 4962 820
Size: 197 926 147 842 867 901 262 1553
Fragments arranged by size:
1553 926 901 867 842 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5695
StyI C'CwwG_G
Cuts at: 513 1065 3134 513
Size: 552 2069 3074
Fragments arranged by size:
3074 2069 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2800 3722 3739 3892 4077 4522 325
Size: 1255 1220 922 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1220 922 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3941 5653 96
Size: 274 80 33 51 1443 1964 1712 138
Fragments arranged by size:
1964 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2440 2476 2440
Size: 36 5659
XhoI C'TCGA_G
Cuts at: 1943 2090 2932 1943
Size: 147 842 4706
Fragments arranged by size:
4706 842 147
XmaI C'CCGG_G
Cuts at: 1870 2800 2938 3020 1870
Size: 930 138 82 4545
Fragments arranged by size:
4545 930 138 82
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI
PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI
SrfI SwaI