Restriction map for
pFN21AE5007
(Circular) (Six-base) MAPSORT of: pFN21AE5007.seq Check: 8491 from: 1 to: 5737
pFN21AE5007 5737 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2393 2393
Size: 5737
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2016 2097 3080 1937
Size: 79 81 983 4594
Fragments arranged by size:
4594 983 81 79
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3800 5184 5452 1632
Size: 2168 1384 268 1917
Fragments arranged by size:
2168 1917 1384 268
AfeI AGC'GCT
Cuts at: 2063 2063
Size: 5737
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2304 2336 2304
Size: 32 5705
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaI G_GGCC'C
Cuts at: 2222 2222
Size: 5737
ApaLI G'TGCA_C
Cuts at: 3794 4950 3794
Size: 1156 4581
ApoI r'AATT_y
Cuts at: 1493 3046 3272 3424 5533 1493
Size: 1553 226 152 2109 1697
Fragments arranged by size:
2109 1697 1553 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 3062 1870
Size: 73 271 848 4545
Fragments arranged by size:
4545 848 271 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2266 2299 2266
Size: 33 5704
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3058 4513 618
Size: 325 217 627 1271 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2184 2222 2774 3056 1391
Size: 793 38 552 282 4072
Fragments arranged by size:
4072 793 552 282 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2614 928
Size: 971 715 4051
Fragments arranged by size:
4051 971 715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3928 3962 1903
Size: 34 50 34 1907 34 3678
Fragments arranged by size:
3678 1907 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2267 3629 4839 2267
Size: 1362 1210 3165
Fragments arranged by size:
3165 1362 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2648 2795 2990 5732 2648
Size: 147 195 2742 2653
Fragments arranged by size:
2742 2653 195 147
BlpI GC'TnA_GC
Cuts at: 3154 3154
Size: 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2222 3798 4954 1790
Size: 432 1576 1156 2573
Fragments arranged by size:
2573 1576 1156 432
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4397 1239
Size: 3158 2579
Bpu10I CC'TnA_GC
Cuts at: 2030 2030
Size: 5737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4727 5025 3862
Size: 865 298 4574
Fragments arranged by size:
4574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2601 2835 4400 882
Size: 617 1102 234 1565 2219
Fragments arranged by size:
2219 1565 1102 617 234
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 5731 5731
Size: 5737
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2145 2154 3544
Size: 53 83 186 936 493 119 9 1390
Cuts at: 3544 3926 4561 275
Size: 382 635 1451
Fragments arranged by size:
1451 1390 936 635 493 382 186 119
83 53 9
BsaWI w'CCGG_w
Cuts at: 4170 4568 4842 4989 5400 4170
Size: 398 274 147 411 4507
Fragments arranged by size:
4507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2683 2713 1178
Size: 30 165 30 1280 30 4202
Fragments arranged by size:
4202 1280 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 4845
BseYI C'CCAG_C
Cuts at: 4940 5436 4940
Size: 496 5241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2386 1163
Size: 573 650 4514
Fragments arranged by size:
4514 650 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2233 3187 3948 4976 1540
Size: 275 186 232 954 761 1028 2301
Fragments arranged by size:
2301 1028 954 761 275 232 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2211 3798 3883 4954 2184
Size: 27 1587 85 1071 2967
Fragments arranged by size:
2967 1587 1071 85 27
BsmI GAATG_Cn'
Cuts at: 3248 3341 3248
Size: 93 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2211 2222 2774 3056 3798 3883
Size: 399 394 27 11 552 282 742 85
Cuts at: 3883 4954 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 394 282 85
27 11
BspEI T'CCGG_A
Cuts at: 5400 5400
Size: 5737
BspHI T'CATG_A
Cuts at: 3626 5356 3626
Size: 1730 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2393 2817 3092 844
Size: 1549 424 275 3489
Fragments arranged by size:
3489 1549 424 275
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4232 4406 65
Size: 1183 2984 174 1396
Fragments arranged by size:
2984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4381 4568 1700
Size: 110 2571 187 2869
Fragments arranged by size:
2869 2571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5737
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 2588 2648 2795 2978 2990 3067 3434 3819
Size: 616 60 147 183 12 77 367 385
Cuts at: 3819 3836 5288 5374 5386 5732 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 616 385 367 346 183 147
86 77 60 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 3253 4047 4067 2266
Size: 987 794 20 3936
Fragments arranged by size:
3936 987 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 2382 3041 2382
Size: 659 5078
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 3184 4073
Size: 54 1425 50 275 18 250 1104 889
Cuts at: 4073 5702 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1104 889 275 250 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3667 1353
Size: 2314 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2182 2182
Size: 5737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3800 4397 5184 5452 1239
Size: 393 2168 597 787 268 1524
Fragments arranged by size:
2168 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2218 2218
Size: 5737
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2988 2988
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 2065 4884 1678
Size: 387 2819 2531
Fragments arranged by size:
2819 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2683 2715 2756 2788 2858
Size: 32 135 32 1111 32 41 32 70
Cuts at: 2858 2890 4378 4410 4452 4484 1373
Size: 32 1488 32 42 32 2626
Fragments arranged by size:
2626 1488 1111 135 70 42 41 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2264 4851 5035 1305
Size: 959 2587 184 2007
Fragments arranged by size:
2587 2007 959 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3695 4054 4213 518
Size: 648 2529 359 159 2042
Fragments arranged by size:
2529 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3153 3830 4978 5223 1606
Size: 1547 677 1148 245 2120
Fragments arranged by size:
2120 1547 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5185
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NruI TCG'CGA
Cuts at: 2229 2229
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 3095 4640 1132
Size: 97 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5737
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5737
PfoI T'CCnGG_A
Cuts at: 2043 2043
Size: 5737
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PspOMI G'GGCC_C
Cuts at: 2218 2218
Size: 5737
PstI C_TGCA'G
Cuts at: 830 3089 830
Size: 2259 3478
PvuI CG_AT'CG
Cuts at: 2001 2233 2001
Size: 232 5505
SacI G_AGCT'C
Cuts at: 2184 2184
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2242 3085 4220 4901 5092 826
Size: 219 1197 843 1135 681 191 1471
Fragments arranged by size:
1471 1197 1135 843 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 2216 3064 1872
Size: 344 848 4545
Fragments arranged by size:
4545 848 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3841 4742 5004 820
Size: 197 926 1898 901 262 1553
Fragments arranged by size:
1898 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 2530 3661 5
Size: 47 2478 1131 2081
Fragments arranged by size:
2478 2081 1131 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 3176 513
Size: 552 2111 3074
Fragments arranged by size:
3074 2111 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2823 3764 3781 3934 4119 4564 325
Size: 1255 1243 941 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1243 941 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3983 5695 96
Size: 274 80 33 51 1443 2006 1712 138
Fragments arranged by size:
2006 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5697 5729 5697
Size: 32 5705
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 2603 2732 2987 3073 2603
Size: 129 255 86 5267
Fragments arranged by size:
5267 255 129 86
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 2214 3062 1870
Size: 344 848 4545
Fragments arranged by size:
4545 848 344
XmnI GAAnn'nnTTC
Cuts at: 3866 3866
Size: 5737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI