Restriction map for
pFN21AE5004
(Circular) (Six-base) MAPSORT of: pFN21AE5004.seq Check: 9592 from: 1 to: 7447
pFN21AE5004 7447 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3935 3935
Size: 7447
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3155 5257 278
Size: 53 83 186 936 1619 2102 2468
Fragments arranged by size:
2468 2102 1619 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2330 3975 4790 1937
Size: 393 1645 815 4594
Fragments arranged by size:
4594 1645 815 393
Acc65I G'GTAC_C
Cuts at: 4768 4768
Size: 7447
AclI AA'CG_TT
Cuts at: 1563 5574 5947 1563
Size: 4011 373 3063
Fragments arranged by size:
4011 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3126 3366 4301 5510 6894 7162 1632
Size: 1494 240 935 1209 1384 268 1917
Fragments arranged by size:
1917 1494 1384 1209 935 268 240
AfeI AGC'GCT
Cuts at: 3458 3458
Size: 7447
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7250
AflIII A'CryG_T
Cuts at: 1203 2728 6346 7323 1203
Size: 1525 3618 977 1327
Fragments arranged by size:
3618 1525 1327 977
AgeI A'CCGG_T
Cuts at: 6278 6278
Size: 7447
AhdI GACnn_n'nnGTC
Cuts at: 4629 6176 4629
Size: 1547 5900
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7447
AlwNI CAG_nnn'CTG
Cuts at: 3950 4331 5190 6762 3950
Size: 381 859 1572 4635
Fragments arranged by size:
4635 1572 859 381
ApaI G_GGCC'C
Cuts at: 2062 3321 3427 2062
Size: 1259 106 6082
Fragments arranged by size:
6082 1259 106
ApaLI G'TGCA_C
Cuts at: 5504 6660 5504
Size: 1156 6291
ApoI r'AATT_y
Cuts at: 1493 2525 2747 3812 4290 4756 4982 5134 7243
Size: 1032 222 1065 478 466 226 152 2109
Cuts at: 7243 1493
Size: 1697
Fragments arranged by size:
2109 1697 1065 1032 478 466 226 222
152
AseI AT'TA_AT
Cuts at: 160 1032 4152 4311 6001 160
Size: 872 3120 159 1690 1606
Fragments arranged by size:
3120 1690 1606 872 159
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
AvaI C'yCGr_G
Cuts at: 1870 1943 4201 4772 1870
Size: 73 2258 571 4545
Fragments arranged by size:
4545 2258 571 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2574 2607 2574
Size: 33 7414
BamHI G'GATC_C
Cuts at: 2397 3601 4777 5144 2397
Size: 1204 1176 367 4700
Fragments arranged by size:
4700 1204 1176 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3290 4768 6223 618
Size: 325 217 627 1503 1478 1455 1842
Fragments arranged by size:
1842 1503 1478 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 2482 3321 3427 4036 4766 1391
Size: 671 420 839 106 609 730 4072
Fragments arranged by size:
4072 839 730 671 609 420 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2267 3252 928
Size: 971 368 985 5123
Fragments arranged by size:
5123 985 971 368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3505 3539 5638 5672 1903
Size: 34 1568 34 2099 34 3678
Fragments arranged by size:
3678 2099 1568 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5339 6549 5339
Size: 1210 6237
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7447
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6058 136
Size: 107 122 71 631 605 4386 1525
Fragments arranged by size:
4386 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4519 7442 4519
Size: 2923 4524
BlpI GC'TnA_GC
Cuts at: 4864 4864
Size: 7447
Bme1580I G_kGCm'C
Cuts at: 1790 2062 3321 3427 5508 6664 1790
Size: 272 1259 106 2081 1156 2573
Fragments arranged by size:
2573 2081 1259 1156 272 106
BmgBI CAC'GTC
Cuts at: 1318 3744 1318
Size: 2426 5021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3600 5178 6136
Size: 416 53 83 399 24 2183 1578 958
Cuts at: 6136 442
Size: 1753
Fragments arranged by size:
2183 1753 1578 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2322 3476 6107 1239
Size: 1083 1154 2631 2579
Fragments arranged by size:
2631 2579 1154 1083
Bpu10I CC'TnA_GC
Cuts at: 3681 3681
Size: 7447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2312 3315 3491 3964 5572 6437 6735 2312
Size: 1003 176 473 1608 865 298 3024
Fragments arranged by size:
3024 1608 1003 865 473 298 176
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2275 2288 6110 882
Size: 617 776 13 3822 2219
Fragments arranged by size:
3822 2219 776 617 13
BsaAI yAC'GTr
Cuts at: 493 1204 5238 493
Size: 711 4034 2702
Fragments arranged by size:
4034 2702 711
BsaBI GATnn'nnATC
Cuts at: 7441 7441
Size: 7447
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3152 5254 5636 6271
Size: 53 83 186 936 1619 2102 382 635
Cuts at: 6271 275
Size: 1451
Fragments arranged by size:
2102 1619 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5880 6278 6552 6699 7110 5880
Size: 398 274 147 411 6217
Fragments arranged by size:
6217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3754 3784 4633 4663 1178
Size: 30 165 30 2351 30 849 30 3962
Fragments arranged by size:
3962 2351 849 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2325 3356 4056 1186
Size: 1139 1031 700 4577
Fragments arranged by size:
4577 1139 1031 700
BseYI C'CCAG_C
Cuts at: 3022 3818 4074 6650 7146 3022
Size: 796 256 2576 496 3323
Fragments arranged by size:
3323 2576 796 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6874
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3176 4897 5658 6686 1540
Size: 275 186 1175 1721 761 1028 2301
Fragments arranged by size:
2301 1721 1175 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4090 5508 5593 6664 4090
Size: 1418 85 1071 4873
Fragments arranged by size:
4873 1418 1071 85
BsmI GAATG_Cn'
Cuts at: 4689 4958 5051 4689
Size: 269 93 7085
Fragments arranged by size:
7085 269 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2482 3321 3427 4036 4090 4766
Size: 399 272 420 839 106 609 54 676
Cuts at: 4766 5508 5593 6664 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 839 742 676 609 420 399
272 106 85 54
BspEI T'CCGG_A
Cuts at: 7110 7110
Size: 7447
BspHI T'CATG_A
Cuts at: 5336 7066 5336
Size: 1730 5717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2561 3139 3935 4802 844
Size: 1717 578 796 867 3489
Fragments arranged by size:
3489 1717 867 796 578
BsrBI CCG'CTC
Cuts at: 1394 1823 5334 1394
Size: 429 3511 3507
Fragments arranged by size:
3511 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2418 2897 3675 4527 4578 5942 6116
Size: 1183 1170 479 778 852 51 1364 174
Cuts at: 6116 65
Size: 1396
Fragments arranged by size:
1396 1364 1183 1170 852 778 479 174
51
BsrFI r'CCGG_y
Cuts at: 1700 1810 6091 6278 1700
Size: 110 4281 187 2869
Fragments arranged by size:
4281 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2753 3067 4092 96
Size: 2657 314 1025 3451
Fragments arranged by size:
3451 2657 1025 314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7447
BssSI C'ACGA_G
Cuts at: 4584 5507 6519 4584
Size: 923 1012 5512
Fragments arranged by size:
5512 1012 923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7447
BstBI TT'CG_AA
Cuts at: 4375 6251 4375
Size: 1876 5571
BstEII G'GTnAC_C
Cuts at: 1184 2173 2963 1184
Size: 989 790 5668
Fragments arranged by size:
5668 989 790
BstXI CCAn_nnnn'nTGG
Cuts at: 3826 4082 3826
Size: 256 7191
BstYI r'GATC_y
Cuts at: 1972 2397 3215 3601 4519 4777 5144 5529 5546
Size: 425 818 386 918 258 367 385 17
Cuts at: 5546 6998 7084 7096 7442 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 918 818 425 386 385 367
346 258 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3537 4348 513
Size: 552 939 1533 811 3612
Fragments arranged by size:
3612 1533 939 811 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6708
Fragments arranged by size:
6708 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 4963 5757 5777 3106
Size: 1857 794 20 4776
Fragments arranged by size:
4776 1857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3268 3303 529
Size: 35 2704 35 4673
Fragments arranged by size:
4673 2704 35 35
DraI TTT'AAA
Cuts at: 4701 4751 4701
Size: 50 7397
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3742 6454 809
Size: 507 2426 2712 1802
Fragments arranged by size:
2712 2426 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4264 4894 5783
Size: 54 1425 50 275 18 2434 630 889
Cuts at: 5783 7412 8
Size: 1629 43
Fragments arranged by size:
2434 1629 1425 889 630 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4894 1537
Size: 275 3082 4090
Fragments arranged by size:
4090 3082 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4259 4428 5377 1353
Size: 2906 169 949 3423
Fragments arranged by size:
3423 2906 949 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4301 4605 6034 6408 6554 387
Size: 1064 138 2712 304 1429 374 146 1280
Fragments arranged by size:
2712 1429 1280 1064 374 304 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2322 3126 3366 3476 4301 5510 6107
Size: 393 690 804 240 110 825 1209 597
Cuts at: 6107 6894 7162 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1209 825 804 787 690 597 393
268 240 110
EcoO109I rG'GnC_Cy
Cuts at: 2058 2059 3027 3317 3318 3423 3424 2058
Size: 1 968 290 1 105 1 6081
Fragments arranged by size:
6081 968 290 105 1 1 1
EcoRI G'AATT_C
Cuts at: 2747 4756 5134 2747
Size: 2009 378 5060
Fragments arranged by size:
5060 2009 378
FspI TGC'GCA
Cuts at: 5953 5953
Size: 7447
HaeII r_GCGC'y
Cuts at: 1678 3460 6594 1678
Size: 1782 3134 2531
Fragments arranged by size:
3134 2531 1782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3011 3043 3332 3364 3474
Size: 32 135 32 1439 32 289 32 110
Cuts at: 3474 3506 4547 4579 4615 4628 4647 4660 6088
Size: 32 1041 32 36 13 19 13 1428
Cuts at: 6088 6120 6162 6194 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1439 1428 1041 289 135 110 42
36 32 32 32 32 32 32 32
32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4048 4791 5037 669
Size: 988 281 2110 743 246 3079
Fragments arranged by size:
3079 2110 988 743 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7447
HpaI GTT'AAC
Cuts at: 4048 5037 4048
Size: 989 6458
KpnI G_GTAC'C
Cuts at: 4772 4772
Size: 7447
MfeI C'AATT_G
Cuts at: 5046 5046
Size: 7447
MluI A'CGCG_T
Cuts at: 7323 7323
Size: 7447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3230 4599 6561 6745 1305
Size: 1925 1369 1962 184 2007
Fragments arranged by size:
2007 1962 1925 1369 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4266 10
Size: 54 1425 343 2434 3191
Fragments arranged by size:
3191 2434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2864 3650 5405 5764 5923 518
Size: 648 1698 786 1755 359 159 2042
Fragments arranged by size:
2042 1755 1698 786 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2616 3165 4735 4863 5540 6688 6933
Size: 477 533 549 1570 128 677 1148 245
Cuts at: 6933 1606
Size: 2120
Fragments arranged by size:
2120 1570 1148 677 549 533 477 245
128
NcoI C'CATG_G
Cuts at: 513 1065 2004 4348 513
Size: 552 939 2344 3612
Fragments arranged by size:
3612 2344 939 552
NdeI CA'TA_TG
Cuts at: 387 3376 387
Size: 2989 4458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7447
NotI GC'GGCC_GC
Cuts at: 4894 4894
Size: 7447
NspI r_CATG'y
Cuts at: 1132 1229 2732 4805 6350 1132
Size: 97 1503 2073 1545 2229
Fragments arranged by size:
2229 2073 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7447
PciI A'CATG_T
Cuts at: 2728 6346 2728
Size: 3618 3829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3212 1108
Size: 2104 5343
PmeI GTTT'AAAC
Cuts at: 4751 4751
Size: 7447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5516 5548 7029 7061 5516
Size: 32 1481 32 5902
Fragments arranged by size:
5902 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3027 3027
Size: 7447
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7447
PsiI TTA'TAA
Cuts at: 5017 5017
Size: 7447
PspOMI G'GGCC_C
Cuts at: 2058 3317 3423 2058
Size: 1259 106 6082
Fragments arranged by size:
6082 1259 106
PstI C_TGCA'G
Cuts at: 830 2907 3012 3136 3164 3328 4734 4799 830
Size: 2077 105 124 28 164 1406 65 3478
Fragments arranged by size:
3478 2077 1406 164 124 105 65 28
PvuI CG_AT'CG
Cuts at: 2001 3176 2001
Size: 1175 6272
PvuII CAG'CTG
Cuts at: 2083 2616 4735 2083
Size: 533 2119 4795
Fragments arranged by size:
4795 2119 533
SalI G'TCGA_C
Cuts at: 1936 4789 1936
Size: 2853 4594
SbfI CC_TGCA'GG
Cuts at: 4799 4799
Size: 7447
ScaI AGT'ACT
Cuts at: 5695 7407 5695
Size: 1712 5735
SfcI C'TryA_G
Cuts at: 826 1045 2205 2739 2903 3008 3132 3160 3324
Size: 219 1160 534 164 105 124 28 164
Cuts at: 3324 4730 4795 5930 6611 6802 826
Size: 1406 65 1135 681 191 1471
Fragments arranged by size:
1471 1406 1160 1135 681 534 219 191
164 164 124 105 65 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7447
SmaI CCC'GGG
Cuts at: 1872 4774 1872
Size: 2902 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 3330 3470 3979 5551 6452
Size: 197 926 348 1039 140 509 1572 901
Cuts at: 6452 6714 820
Size: 262 1553
Fragments arranged by size:
1572 1553 1039 926 901 509 348 262
197 140
SnaBI TAC'GTA
Cuts at: 493 5238 493
Size: 4745 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7447
SphI G_CATG'C
Cuts at: 1132 4805 1132
Size: 3673 3774
SspI AAT'ATT
Cuts at: 5 52 3844 4663 5371 5
Size: 47 3792 819 708 2081
Fragments arranged by size:
3792 2081 819 708 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7447
StyI C'CwwG_G
Cuts at: 513 1065 2004 3850 3921 4348 4886 513
Size: 552 939 1846 71 427 538 3074
Fragments arranged by size:
3074 1846 939 552 538 427 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3252 5474 5491 5644 5829 6274 325
Size: 1255 1672 2222 17 153 185 445 1498
Fragments arranged by size:
2222 1672 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 2753 3067
Size: 274 80 33 51 1443 531 245 314
Cuts at: 3067 4092 4382 5693 7405 96
Size: 1025 290 1311 1712 138
Fragments arranged by size:
1712 1443 1311 1025 531 314 290 274
245 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7407 7439 7407
Size: 32 7415
Tth111I GACn'n_nGTC
Cuts at: 1316 3971 1316
Size: 2655 4792
XbaI T'CTAG_A
Cuts at: 2050 4783 2050
Size: 2733 4714
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3509 3509
Size: 7447
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7447
XmaI C'CCGG_G
Cuts at: 1870 4772 1870
Size: 2902 4545
XmnI GAAnn'nnTTC
Cuts at: 4422 5576 4422
Size: 1154 6293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3153 5255 276
Size: 53 83 186 936 1619 2102 2468
Fragments arranged by size:
2468 2102 1619 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI