Restriction map for
pFN21AE5001
(Circular) (Six-base) MAPSORT of: pFN21AE5001.seq Check: 2441 from: 1 to: 7852
pFN21AE5001 7852 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5662 278
Size: 53 83 186 936 4126 2468
Fragments arranged by size:
4126 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2893 5195 1937
Size: 956 2302 4594
Fragments arranged by size:
4594 2302 956
Acc65I G'GTAC_C
Cuts at: 5173 5173
Size: 7852
AclI AA'CG_TT
Cuts at: 1563 5979 6352 1563
Size: 4416 373 3063
Fragments arranged by size:
4416 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2894 3047 3294 4349 5915 7299 7567
Size: 416 846 153 247 1055 1566 1384 268
Cuts at: 7567 1632
Size: 1917
Fragments arranged by size:
1917 1566 1384 1055 846 416 268 247
153
AflII C'TTAA_G
Cuts at: 820 1017 4193 820
Size: 197 3176 4479
Fragments arranged by size:
4479 3176 197
AflIII A'CryG_T
Cuts at: 1203 2334 6751 7728 1203
Size: 1131 4417 977 1327
Fragments arranged by size:
4417 1327 1131 977
AgeI A'CCGG_T
Cuts at: 6683 6683
Size: 7852
AhdI GACnn_n'nnGTC
Cuts at: 3275 4581 6581 3275
Size: 1306 2000 4546
Fragments arranged by size:
4546 2000 1306
AleI CACnn'nnGTG
Cuts at: 1166 4436 1166
Size: 3270 4582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3442 1184
Size: 2258 5594
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3363 3395 3363
Size: 32 7820
AlwNI CAG_nnn'CTG
Cuts at: 2959 3382 3805 5595 7167 2959
Size: 423 423 1790 1572 3644
Fragments arranged by size:
3644 1790 1572 423 423
ApaLI G'TGCA_C
Cuts at: 4363 5909 7065 4363
Size: 1546 1156 5150
Fragments arranged by size:
5150 1546 1156
ApoI r'AATT_y
Cuts at: 1493 2037 2178 2760 3005 3123 3614 3878 3982
Size: 544 141 582 245 118 491 264 104
Cuts at: 3982 4900 5161 5387 5539 7648 1493
Size: 918 261 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 582 544 491 264 261
245 226 152 141 118 104
AseI AT'TA_AT
Cuts at: 160 1032 6406 160
Size: 872 5374 1606
Fragments arranged by size:
5374 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7852
AvaI C'yCGr_G
Cuts at: 1870 1943 4787 5177 1870
Size: 73 2844 390 4545
Fragments arranged by size:
4545 2844 390 73
BamHI G'GATC_C
Cuts at: 5182 5549 5182
Size: 367 7485
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5173 6628 618
Size: 325 217 627 3386 1455 1842
Fragments arranged by size:
3386 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4065 5171 1391
Size: 2674 1106 4072
Fragments arranged by size:
4072 2674 1106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4761 928
Size: 971 2862 4019
Fragments arranged by size:
4019 2862 971
BbvCI CC'TCA_GC
Cuts at: 3245 4121 3245
Size: 876 6976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4334 4368 6043 6077 1903
Size: 34 2397 34 1675 34 3678
Fragments arranged by size:
3678 2397 1675 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5744 6954 5744
Size: 1210 6642
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7852
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6463 136
Size: 107 122 71 631 605 4791 1525
Fragments arranged by size:
4791 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3283 4510 7847 3283
Size: 1227 3337 3288
Fragments arranged by size:
3337 3288 1227
BlpI GC'TnA_GC
Cuts at: 4734 4850 5269 4734
Size: 116 419 7317
Fragments arranged by size:
7317 419 116
Bme1580I G_kGCm'C
Cuts at: 1790 2922 4367 5913 7069 1790
Size: 1132 1445 1546 1156 2573
Fragments arranged by size:
2573 1546 1445 1156 1132
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7852
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2327 5583 6541
Size: 416 53 83 399 24 910 3256 958
Cuts at: 6541 442
Size: 1753
Fragments arranged by size:
3256 1753 958 910 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7852
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3879 3879
Size: 7852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 2936 4026 4073 4991 6512 1239
Size: 966 731 1090 47 918 1521 2579
Fragments arranged by size:
2579 1521 1090 966 918 731 47
Bpu10I CC'TnA_GC
Cuts at: 3245 4121 3245
Size: 876 6976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2489 4163 4997 5063 5977 6842 7140 2489
Size: 1674 834 66 914 865 298 3201
Fragments arranged by size:
3201 1674 914 865 834 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6515 882
Size: 617 5016 2219
Fragments arranged by size:
5016 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5643 493
Size: 711 4439 2702
Fragments arranged by size:
4439 2702 711
BsaBI GATnn'nnATC
Cuts at: 7846 7846
Size: 7852
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5659 6041 6676 275
Size: 53 83 186 936 4126 382 635 1451
Fragments arranged by size:
4126 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5013 6285 6683 6957 7104 7515 5013
Size: 1272 398 274 147 411 5350
Fragments arranged by size:
5350 1272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3845 3875 3901 3931 1178
Size: 30 165 30 2442 30 26 30 5099
Fragments arranged by size:
5099 2442 165 30 30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7852
BseYI C'CCAG_C
Cuts at: 2248 3023 4708 7055 7551 2248
Size: 775 1685 2347 496 2549
Fragments arranged by size:
2549 2347 1685 775 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2140 1163
Size: 573 404 6875
Fragments arranged by size:
6875 573 404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5151 5302 6063 7091 1540
Size: 275 186 3150 151 761 1028 2301
Fragments arranged by size:
3150 2301 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2631 4367 4462 5913 5998 7069 2631
Size: 1736 95 1451 85 1071 3414
Fragments arranged by size:
3414 1736 1451 1071 95 85
BsmI GAATG_Cn'
Cuts at: 5363 5456 5363
Size: 93 7759
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2631 2922 4065 4367 4462 5171 5913
Size: 399 841 291 1143 302 95 709 742
Cuts at: 5913 5998 7069 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1143 1071 841 742 709 399 302
291 95 85
BspEI T'CCGG_A
Cuts at: 5013 7515 5013
Size: 2502 5350
BspHI T'CATG_A
Cuts at: 5741 7471 5741
Size: 1730 6122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5207 844
Size: 4363 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5739 1394
Size: 429 3916 3507
Fragments arranged by size:
3916 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6347 6521 65
Size: 1183 5099 174 1396
Fragments arranged by size:
5099 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3660 6496 6683 1700
Size: 110 1850 2836 187 2869
Fragments arranged by size:
2869 2836 1850 187 110
BsrGI T'GTAC_A
Cuts at: 96 4494 96
Size: 4398 3454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7852
BssSI C'ACGA_G
Cuts at: 5912 6924 5912
Size: 1012 6840
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7852
BstBI TT'CG_AA
Cuts at: 6656 6656
Size: 7852
BstEII G'GTnAC_C
Cuts at: 1184 4803 1184
Size: 3619 4233
BstXI CCAn_nnnn'nTGG
Cuts at: 5079 5079
Size: 7852
BstYI r'GATC_y
Cuts at: 1972 2844 2962 3040 3283 3499 4261 4489 4510
Size: 872 118 78 243 216 762 228 21
Cuts at: 4510 5182 5549 5934 5951 7403 7489 7501 7847
Size: 672 367 385 17 1452 86 12 346
Cuts at: 7847 1972
Size: 1977
Fragments arranged by size:
1977 1452 872 762 672 385 367 346
243 228 216 118 86 78 21 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3849 513
Size: 552 939 1845 4516
Fragments arranged by size:
4516 1845 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7113
Fragments arranged by size:
7113 683 56
BtsI GCAGTG_nn'
Cuts at: 3616 5090 5368 6162 6182 3616
Size: 1474 278 794 20 5286
Fragments arranged by size:
5286 1474 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 2286 1554
Size: 732 7120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7817
DraI TTT'AAA
Cuts at: 2391 3151 3265 5156 2391
Size: 760 114 1891 5087
Fragments arranged by size:
5087 1891 760 114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6859 809
Size: 507 5543 1802
Fragments arranged by size:
5543 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 2982 4050
Size: 54 1425 50 275 18 687 465 1068
Cuts at: 4050 4428 4743 5048 5103 5299 6188 7817 8
Size: 378 315 305 55 196 889 1629 43
Fragments arranged by size:
1629 1425 1068 889 687 465 378 315
305 275 196 55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5299 1537
Size: 275 3487 4090
Fragments arranged by size:
4090 3487 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2913 3687 3835 5782 1353
Size: 671 889 774 148 1947 3423
Fragments arranged by size:
3423 1947 889 774 671 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6439 6813 6959 387
Size: 1064 138 4850 374 146 1280
Fragments arranged by size:
4850 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2205 2894 2936 3047 3294 4026
Size: 393 416 157 689 42 111 247 732
Cuts at: 4026 4073 4349 4991 5915 6512 7299 7567 1239
Size: 47 276 642 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 732 689 642 597 416
393 276 268 247 157 111 47 42
EcoO109I rG'GnC_Cy
Cuts at: 2437 3379 2437
Size: 942 6910
EcoRI G'AATT_C
Cuts at: 3614 3878 5161 5539 3614
Size: 264 1283 378 5927
Fragments arranged by size:
5927 1283 378 264
EcoRV GAT'ATC
Cuts at: 4256 4256
Size: 7852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4318 4318
Size: 7852
FspI TGC'GCA
Cuts at: 6358 6358
Size: 7852
HaeII r_GCGC'y
Cuts at: 1678 6999 1678
Size: 5321 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3129 3161 3261 3293 3484
Size: 32 135 32 1557 32 100 32 191
Cuts at: 3484 3516 3845 3877 4142 4174 4253 4285 4567
Size: 32 329 32 265 32 79 32 282
Cuts at: 4567 4599 6493 6525 6567 6599 1373
Size: 32 1894 32 42 32 2626
Fragments arranged by size:
2626 1894 1557 329 282 265 191 135
100 79 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4399 5196 5442 669
Size: 988 281 2461 797 246 3079
Fragments arranged by size:
3079 2461 988 797 281 246
HindIII A'AGCT_T
Cuts at: 748 2368 3163 748
Size: 1620 795 5437
Fragments arranged by size:
5437 1620 795
HpaI GTT'AAC
Cuts at: 4399 5442 4399
Size: 1043 6809
KpnI G_GTAC'C
Cuts at: 5177 5177
Size: 7852
MfeI C'AATT_G
Cuts at: 3035 5451 3035
Size: 2416 5436
MluI A'CGCG_T
Cuts at: 7728 7728
Size: 7852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3643 3678 4058 4185 6966 7150 1305
Size: 2338 35 380 127 2781 184 2007
Fragments arranged by size:
2781 2338 2007 380 184 127 35
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 2984 4052 4430 4745
Size: 54 1425 343 687 465 1068 378 315
Cuts at: 4745 5050 5105 10
Size: 305 55 2757
Fragments arranged by size:
2757 1425 1068 687 465 378 343 315
305 55 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3897 4436 4568 5810 6169 6328 518
Size: 648 2731 539 132 1242 359 159 2042
Fragments arranged by size:
2731 2042 1242 648 539 359 159 132
MspA1I CmG'CkG
Cuts at: 1606 4712 5268 5945 7093 7338 1606
Size: 3106 556 677 1148 245 2120
Fragments arranged by size:
3106 2120 1148 677 556 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3849 513
Size: 552 939 1845 4516
Fragments arranged by size:
4516 1845 939 552
NdeI CA'TA_TG
Cuts at: 387 3765 387
Size: 3378 4474
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7852
NotI GC'GGCC_GC
Cuts at: 5299 5299
Size: 7852
NspI r_CATG'y
Cuts at: 1132 1229 2338 2918 3253 5210 6755 1132
Size: 97 1109 580 335 1957 1545 2229
Fragments arranged by size:
2229 1957 1545 1109 580 335 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7852
PciI A'CATG_T
Cuts at: 2334 6751 2334
Size: 4417 3435
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2959 3382 1108
Size: 1851 423 5578
Fragments arranged by size:
5578 1851 423
PfoI T'CCnGG_A
Cuts at: 2182 2952 3381 2182
Size: 770 429 6653
Fragments arranged by size:
6653 770 429
PmeI GTTT'AAAC
Cuts at: 5156 5156
Size: 7852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2682 2714 5921 5953 7434 7466 2682
Size: 32 3207 32 1481 32 3068
Fragments arranged by size:
3207 3068 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3379 3379
Size: 7852
PshAI GACnn'nnGTC
Cuts at: 1613 4142 1613
Size: 2529 5323
PsiI TTA'TAA
Cuts at: 2796 3230 5422 2796
Size: 434 2192 5226
Fragments arranged by size:
5226 2192 434
PstI C_TGCA'G
Cuts at: 830 2355 3801 5204 830
Size: 1525 1446 1403 3478
Fragments arranged by size:
3478 1525 1446 1403
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7852
PvuII CAG'CTG
Cuts at: 4712 4712
Size: 7852
SalI G'TCGA_C
Cuts at: 1936 5194 1936
Size: 3258 4594
SapI GCTCTTCn'nnn_
Cuts at: 3687 3687
Size: 7852
SbfI CC_TGCA'GG
Cuts at: 5204 5204
Size: 7852
ScaI AGT'ACT
Cuts at: 6100 7812 6100
Size: 1712 6140
SfcI C'TryA_G
Cuts at: 826 1045 2351 2840 3357 3797 5125 5200 6335
Size: 219 1306 489 517 440 1328 75 1135
Cuts at: 6335 7016 7207 826
Size: 681 191 1471
Fragments arranged by size:
1471 1328 1306 1135 681 517 489 440
219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7852
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7852
SmaI CCC'GGG
Cuts at: 1872 5179 1872
Size: 3307 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2468 4178 4193 4787 4976 5042
Size: 197 926 525 1710 15 594 189 66
Cuts at: 5042 5956 6857 7119 820
Size: 914 901 262 1553
Fragments arranged by size:
1710 1553 926 914 901 594 525 262
197 189 66 15
SnaBI TAC'GTA
Cuts at: 493 5643 493
Size: 5150 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7852
SphI G_CATG'C
Cuts at: 1132 5210 1132
Size: 4078 3774
SspI AAT'ATT
Cuts at: 5 52 5776 5
Size: 47 5724 2081
Fragments arranged by size:
5724 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7852
StyI C'CwwG_G
Cuts at: 513 1065 2004 2077 3495 3849 5291 513
Size: 552 939 73 1418 354 1442 3074
Fragments arranged by size:
3074 1442 1418 939 552 354 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4733 5085 5879 5896 6049 6234 6679
Size: 1255 3153 352 794 17 153 185 445
Cuts at: 6679 325
Size: 1498
Fragments arranged by size:
3153 1498 1255 794 445 352 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4494 6098 7810
Size: 274 80 33 51 1443 2517 1604 1712
Cuts at: 7810 96
Size: 138
Fragments arranged by size:
2517 1712 1604 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3901 3933 7812 7844 3901
Size: 32 3879 32 3909
Fragments arranged by size:
3909 3879 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7852
XbaI T'CTAG_A
Cuts at: 5188 5188
Size: 7852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3602 3847 3602
Size: 245 7607
XhoI C'TCGA_G
Cuts at: 1943 4787 1943
Size: 2844 5008
XmaI C'CCGG_G
Cuts at: 1870 5177 1870
Size: 3307 4545
XmnI GAAnn'nnTTC
Cuts at: 3696 5981 3696
Size: 2285 5567
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5660 276
Size: 53 83 186 936 4126 2468
Fragments arranged by size:
4126 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI