Restriction map for
pFN21AE4596
(Circular) (Six-base) MAPSORT of: pFN21AE4596.seq Check: 1503 from: 1 to: 8443
pFN21AE4596 8443 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6253 278
Size: 53 83 186 936 4717 2468
Fragments arranged by size:
4717 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4422 5786 1937
Size: 2485 1364 4594
Fragments arranged by size:
4594 2485 1364
Acc65I G'GTAC_C
Cuts at: 5764 5764
Size: 8443
AclI AA'CG_TT
Cuts at: 1563 6570 6943 1563
Size: 5007 373 3063
Fragments arranged by size:
5007 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3434 3940 4532 4752 4819 5099 6506 7890
Size: 1802 506 592 220 67 280 1407 1384
Cuts at: 7890 8158 1632
Size: 268 1917
Fragments arranged by size:
1917 1802 1407 1384 592 506 280 268
220 67
AfeI AGC'GCT
Cuts at: 3821 3821
Size: 8443
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8246
AflIII A'CryG_T
Cuts at: 1203 3692 3873 5413 7342 8319 1203
Size: 2489 181 1540 1929 977 1327
Fragments arranged by size:
2489 1929 1540 1327 977 181
AgeI A'CCGG_T
Cuts at: 7274 7274
Size: 8443
AhdI GACnn_n'nnGTC
Cuts at: 7172 7172
Size: 8443
AleI CACnn'nnGTG
Cuts at: 1166 4796 1166
Size: 3630 4813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8443
AlwNI CAG_nnn'CTG
Cuts at: 2344 2621 3868 4360 4679 4906 5077 5611 6186
Size: 277 1247 492 319 227 171 534 575
Cuts at: 6186 7758 2344
Size: 1572 3029
Fragments arranged by size:
3029 1572 1247 575 534 492 319 277
227 171
ApaI G_GGCC'C
Cuts at: 3644 5011 5536 3644
Size: 1367 525 6551
Fragments arranged by size:
6551 1367 525
ApaLI G'TGCA_C
Cuts at: 3905 6500 7656 3905
Size: 2595 1156 4692
Fragments arranged by size:
4692 2595 1156
ApoI r'AATT_y
Cuts at: 1493 4856 5483 5500 5752 5978 6130 8239 1493
Size: 3363 627 17 252 226 152 2109 1697
Fragments arranged by size:
3363 2109 1697 627 252 226 152 17
AseI AT'TA_AT
Cuts at: 160 1032 6997 160
Size: 872 5965 1606
Fragments arranged by size:
5965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8443
AvaI C'yCGr_G
Cuts at: 1870 1943 2760 3531 3729 4175 5535 5768 1870
Size: 73 817 771 198 446 1360 233 4545
Fragments arranged by size:
4545 1360 817 771 446 233 198 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2793 2826 2793
Size: 33 8410
BamHI G'GATC_C
Cuts at: 2260 4867 5773 6140 2260
Size: 2607 906 367 4563
Fragments arranged by size:
4563 2607 906 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4142 4687 5672 5764 7219
Size: 325 217 627 2355 545 985 92 1455
Cuts at: 7219 618
Size: 1842
Fragments arranged by size:
2355 1842 1455 985 627 545 325 217
92
BanII G_rGCy'C
Cuts at: 1391 2076 2119 2293 3513 3644 3885 5004 5011
Size: 685 43 174 1220 131 241 1119 7
Cuts at: 5011 5041 5286 5363 5536 5568 5762 1391
Size: 30 245 77 173 32 194 4072
Fragments arranged by size:
4072 1220 1119 685 245 241 194 174
173 131 77 43 32 30 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2261 2784 928
Size: 971 362 523 6587
Fragments arranged by size:
6587 971 523 362
BbvCI CC'TCA_GC
Cuts at: 2523 2523
Size: 8443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6634 6668 1903
Size: 34 4697 34 3678
Fragments arranged by size:
4697 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2469 2821 6335 7545 2469
Size: 352 3514 1210 3367
Fragments arranged by size:
3514 3367 1210 352
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2419 3840 4735
Size: 107 122 71 631 605 747 1421 895
Cuts at: 4735 7054 136
Size: 2319 1525
Fragments arranged by size:
2319 1525 1421 895 747 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2161 2500 2548 3426 4821 8438 2161
Size: 339 48 878 1395 3617 2166
Fragments arranged by size:
3617 2166 1395 878 339 48
BlpI GC'TnA_GC
Cuts at: 5860 5860
Size: 8443
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2158 3462 3644 3700 3909 4176 4692
Size: 301 67 1304 182 56 209 267 516
Cuts at: 4692 5011 5222 5536 6504 7660 1790
Size: 319 211 314 968 1156 2573
Fragments arranged by size:
2573 1304 1156 968 516 319 314 301
267 211 209 182 67 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 2288 2354
Size: 416 53 83 399 24 684 187 66
Cuts at: 2354 2425 3694 4225 5065 6174 7132 442
Size: 71 1269 531 840 1109 958 1753
Fragments arranged by size:
1753 1269 1109 958 840 684 531 416
399 187 83 71 66 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3769 4342 3769
Size: 573 7870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2022 2103 2133 2387 2400 2598 3275 3485
Size: 783 81 30 254 13 198 677 210
Cuts at: 3485 4748 5039 7103 1239
Size: 1263 291 2064 2579
Fragments arranged by size:
2579 2064 1263 783 677 291 254 210
198 81 30 13
Bpu10I CC'TnA_GC
Cuts at: 2523 2636 3098 4920 2523
Size: 113 462 1822 6046
Fragments arranged by size:
6046 1822 462 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3407 3917 5381 6568 7433 7731 3407
Size: 510 1464 1187 865 298 4119
Fragments arranged by size:
4119 1464 1187 865 510 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2274 3986 4457 4594 7106 882
Size: 617 775 1712 471 137 2512 2219
Fragments arranged by size:
2512 2219 1712 775 617 471 137
BsaAI yAC'GTr
Cuts at: 493 1204 3695 4245 5206 6234 493
Size: 711 2491 550 961 1028 2702
Fragments arranged by size:
2702 2491 1028 961 711 550
BsaBI GATnn'nnATC
Cuts at: 2892 8437 2892
Size: 5545 2898
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2868 6250 6632 7267
Size: 53 83 186 936 1335 3382 382 635
Cuts at: 7267 275
Size: 1451
Fragments arranged by size:
3382 1451 1335 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3175 6876 7274 7548 7695 8106 3175
Size: 3701 398 274 147 411 3512
Fragments arranged by size:
3701 3512 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2280 2310 2602 2632 2858
Size: 30 165 30 877 30 292 30 226
Cuts at: 2858 2888 4046 4076 5649 5679 1178
Size: 30 1158 30 1573 30 3942
Fragments arranged by size:
3942 1573 1158 877 292 226 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2531 2540 2543 3332 3549 4321 4409 4582
Size: 1345 9 3 789 217 772 88 173
Cuts at: 4582 5036 5264 5336 1186
Size: 454 228 72 4293
Fragments arranged by size:
4293 1345 789 772 454 228 217 173
88 72 9 3
BseYI C'CCAG_C
Cuts at: 2200 2284 2907 4218 4381 4443 4467 4930 5372
Size: 84 623 1311 163 62 24 463 442
Cuts at: 5372 5668 7646 8142 2200
Size: 296 1978 496 2501
Fragments arranged by size:
2501 1978 1311 623 496 463 442 296
163 84 62 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2948 3630 3854 4118 4175 1163
Size: 573 1212 682 224 264 57 5431
Fragments arranged by size:
5431 1212 682 573 264 224 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5893 6654 7682 1540
Size: 275 186 3892 761 1028 2301
Fragments arranged by size:
3892 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2119 2232 2752 3471 3513 3885 3909 4803
Size: 43 113 520 719 42 372 24 894
Cuts at: 4803 5190 5286 5309 6504 6589 7660 2076
Size: 387 96 23 1195 85 1071 2859
Fragments arranged by size:
2859 1195 1071 894 719 520 387 372
113 96 85 43 42 24 23
BsmI GAATG_Cn'
Cuts at: 5100 5601 5954 6047 5100
Size: 501 353 93 7496
Fragments arranged by size:
7496 501 353 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2091 2119 2158 2232 2293 2752
Size: 399 286 15 28 39 74 61 459
Cuts at: 2752 3462 3471 3513 3644 3700 3885 3909 4176
Size: 710 9 42 131 56 185 24 267
Cuts at: 4176 4692 4803 5004 5011 5041 5190 5222 5286
Size: 516 111 201 7 30 149 32 64
Cuts at: 5286 5309 5363 5536 5568 5762 6504 6589 7660
Size: 23 54 173 32 194 742 85 1071
Cuts at: 7660 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 710 516 459 399 286
267 201 194 185 173 149 131 111
85 74 64 61 56 54 42 39
32 32 30 28 24 23 15 9
7
BspEI T'CCGG_A
Cuts at: 3175 8106 3175
Size: 4931 3512
BspHI T'CATG_A
Cuts at: 3195 6332 8062 3195
Size: 3137 1730 3576
Fragments arranged by size:
3576 3137 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4086 4197 4434 4974 5798 844
Size: 3242 111 237 540 824 3489
Fragments arranged by size:
3489 3242 824 540 237 111
BsrBI CCG'CTC
Cuts at: 1394 1823 5119 6330 1394
Size: 429 3296 1211 3507
Fragments arranged by size:
3507 3296 1211 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3039 5147 6938 7112 65
Size: 1183 1791 2108 1791 174 1396
Fragments arranged by size:
2108 1791 1791 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2409 7087 7274 1700
Size: 110 599 4678 187 2869
Fragments arranged by size:
4678 2869 599 187 110
BsrGI T'GTAC_A
Cuts at: 96 3163 3225 5047 96
Size: 3067 62 1822 3492
Fragments arranged by size:
3492 3067 1822 62
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8443
BssSI C'ACGA_G
Cuts at: 4706 6503 7515 4706
Size: 1797 1012 5634
Fragments arranged by size:
5634 1797 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8443
BstBI TT'CG_AA
Cuts at: 7247 7247
Size: 8443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8443
BstXI CCAn_nnnn'nTGG
Cuts at: 2863 3046 3611 4381 5363 2863
Size: 183 565 770 982 5943
Fragments arranged by size:
5943 982 770 565 183
BstYI r'GATC_y
Cuts at: 1972 2041 2161 2260 2500 2548 3207 3426 3801
Size: 69 120 99 240 48 659 219 375
Cuts at: 3801 4821 4867 5773 6140 6525 6542 7994 8080
Size: 1020 46 906 367 385 17 1452 86
Cuts at: 8080 8092 8438 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1020 906 659 385 375 367
346 240 219 120 99 86 69 48
46 17 12
Bsu36I CC'TnA_GG
Cuts at: 3754 4283 5319 5580 3754
Size: 529 1036 261 6617
Fragments arranged by size:
6617 1036 529 261
BtgI C'CryG_G
Cuts at: 513 1065 2067 2137 3655 3708 3838 3961 4002
Size: 552 1002 70 1518 53 130 123 41
Cuts at: 4002 4752 513
Size: 750 4204
Fragments arranged by size:
4204 1518 1002 750 552 130 123 70
53 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7704
Fragments arranged by size:
7704 683 56
BtsI GCAGTG_nn'
Cuts at: 2700 2752 2855 4677 4890 5630 5959 6753 6773
Size: 52 103 1822 213 740 329 794 20
Cuts at: 6773 2700
Size: 4370
Fragments arranged by size:
4370 1822 794 740 329 213 103 52
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8408
DraI TTT'AAA
Cuts at: 5747 5747
Size: 8443
DraIII CAC_nnn'GTG
Cuts at: 4753 4753
Size: 8443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7450 809
Size: 507 6134 1802
Fragments arranged by size:
6134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2468 4411 5427
Size: 54 1425 50 275 18 638 1943 1016
Cuts at: 5427 5724 5890 6779 8408 8
Size: 297 166 889 1629 43
Fragments arranged by size:
1943 1629 1425 1016 889 638 297 275
166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5890 1537
Size: 275 4078 4090
Fragments arranged by size:
4090 4078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2409 3788 3815 3959 4298 6373 1353
Size: 1056 1379 27 144 339 2075 3423
Fragments arranged by size:
3423 2075 1379 1056 339 144 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4226 7030 7404 7550 387
Size: 1064 138 2546 91 2804 374 146 1280
Fragments arranged by size:
2804 2546 1280 1064 374 146 138 91
EcoICRI GAG'CTC
Cuts at: 2074 2117 3511 3883 5284 2074
Size: 43 1394 372 1401 5233
Fragments arranged by size:
5233 1401 1394 372 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2103 2133 2387 2400 2598 3275
Size: 393 390 81 30 254 13 198 677
Cuts at: 3275 3434 3485 3940 4532 4748 4752 4819 5039
Size: 159 51 455 592 216 4 67 220
Cuts at: 5039 5099 6506 7103 7890 8158 1239
Size: 60 1407 597 787 268 1524
Fragments arranged by size:
1524 1407 787 677 597 592 455 393
390 268 254 220 216 198 159 81
67 60 51 30 13 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3971 5399 3971
Size: 1428 7015
EcoO109I rG'GnC_Cy
Cuts at: 2711 2824 3094 3250 3640 3641 3865 4075 4126
Size: 113 270 156 390 1 224 210 51
Cuts at: 4126 4357 4654 4736 5574 5584 2711
Size: 231 297 82 838 10 5570
Fragments arranged by size:
5570 838 390 297 270 231 224 210
156 113 82 51 10 1
EcoRI G'AATT_C
Cuts at: 5752 6130 5752
Size: 378 8065
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2570 4327 2570
Size: 1757 6686
FspI TGC'GCA
Cuts at: 4049 6949 4049
Size: 2900 5543
HaeII r_GCGC'y
Cuts at: 1678 3823 5611 7590 1678
Size: 2145 1788 1979 2531
Fragments arranged by size:
2531 2145 1979 1788
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2280 2284 2312 2822
Size: 32 135 32 680 28 4 28 510
Cuts at: 2822 2854 2858 2890 7084 7116 7158 7190 1373
Size: 32 4 32 4194 32 42 32 2626
Fragments arranged by size:
4194 2626 680 510 135 42 32 32
32 32 32 32 28 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4423 5787 6033 669
Size: 988 281 2485 1364 246 3079
Fragments arranged by size:
3079 2485 1364 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8443
HpaI GTT'AAC
Cuts at: 6033 6033
Size: 8443
KpnI G_GTAC'C
Cuts at: 5768 5768
Size: 8443
MfeI C'AATT_G
Cuts at: 6042 6042
Size: 8443
MluI A'CGCG_T
Cuts at: 8319 8319
Size: 8443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2328 2474 2628 2633 5118 7557 7741 1305
Size: 1023 146 154 5 2485 2439 184 2007
Fragments arranged by size:
2485 2439 2007 1023 184 154 146 5
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2470 4413 5429 10
Size: 54 1425 343 638 1943 1016 3024
Fragments arranged by size:
3024 1943 1425 1016 638 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 4202 4796 6401 6760 6919 518
Size: 648 1695 1341 594 1605 359 159 2042
Fragments arranged by size:
2042 1695 1605 1341 648 594 359 159
MspA1I CmG'CkG
Cuts at: 1606 2807 3169 3566 3657 3738 3856 4222 4443
Size: 1201 362 397 91 81 118 366 221
Cuts at: 4443 5598 5690 5859 6536 7684 7929 1606
Size: 1155 92 169 677 1148 245 2120
Fragments arranged by size:
2120 1201 1155 1148 677 397 366 362
245 221 169 118 92 91 81
NaeI GCC'GGC
Cuts at: 2411 2411
Size: 8443
NcoI C'CATG_G
Cuts at: 513 1065 2137 4002 513
Size: 552 1072 1865 4954
Fragments arranged by size:
4954 1865 1072 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8443
NgoMIV G'CCGG_C
Cuts at: 2409 2409
Size: 8443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8443
NotI GC'GGCC_GC
Cuts at: 5890 5890
Size: 8443
NspI r_CATG'y
Cuts at: 1132 1229 3877 4018 5545 5801 7346 1132
Size: 97 2648 141 1527 256 1545 2229
Fragments arranged by size:
2648 2229 1545 1527 256 141 97
PasI CC'CwG_GG
Cuts at: 1348 3502 4085 5197 5363 1348
Size: 2154 583 1112 166 4428
Fragments arranged by size:
4428 2154 1112 583 166
PciI A'CATG_T
Cuts at: 3873 7342 3873
Size: 3469 4974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 2621 4753 5476 1108
Size: 1236 277 2132 723 4075
Fragments arranged by size:
4075 2132 1236 723 277
PfoI T'CCnGG_A
Cuts at: 2620 3114 4071 4899 2620
Size: 494 957 828 6164
Fragments arranged by size:
6164 957 828 494
PmeI GTTT'AAAC
Cuts at: 5747 5747
Size: 8443
PmlI CAC'GTG
Cuts at: 3695 3695
Size: 8443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6512 6544 8025 8057 6512
Size: 32 1481 32 6898
Fragments arranged by size:
6898 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2711 3250 3865 4075 4654 5574 2711
Size: 539 615 210 579 920 5580
Fragments arranged by size:
5580 920 615 579 539 210
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8443
PsiI TTA'TAA
Cuts at: 6013 6013
Size: 8443
PspOMI G'GGCC_C
Cuts at: 3640 5007 5532 3640
Size: 1367 525 6551
Fragments arranged by size:
6551 1367 525
PstI C_TGCA'G
Cuts at: 830 2169 2747 3013 3819 4227 4684 4971 5795
Size: 1339 578 266 806 408 457 287 824
Cuts at: 5795 830
Size: 3478
Fragments arranged by size:
3478 1339 824 806 578 457 408 287
266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8443
PvuII CAG'CTG
Cuts at: 2807 3169 3566 3856 4222 4443 2807
Size: 362 397 290 366 221 6807
Fragments arranged by size:
6807 397 366 362 290 221
SacI G_AGCT'C
Cuts at: 2076 2119 3513 3885 5286 2076
Size: 43 1394 372 1401 5233
Fragments arranged by size:
5233 1401 1394 372 43
SacII CC_GC'GG
Cuts at: 3658 3658
Size: 8443
SalI G'TCGA_C
Cuts at: 1936 4421 5785 1936
Size: 2485 1364 4594
Fragments arranged by size:
4594 2485 1364
SanDI GG'GwC_CC
Cuts at: 5574 5574
Size: 8443
SapI GCTCTTCn'nnn_
Cuts at: 3788 3959 3788
Size: 171 8272
SbfI CC_TGCA'GG
Cuts at: 5795 5795
Size: 8443
ScaI AGT'ACT
Cuts at: 6691 8403 6691
Size: 1712 6731
SexAI A'CCwGG_T
Cuts at: 2566 2566
Size: 8443
SfcI C'TryA_G
Cuts at: 826 1045 2165 2309 2743 3009 3815 4223 4583
Size: 219 1120 144 434 266 806 408 360
Cuts at: 4583 4680 4967 5791 6926 7607 7798 826
Size: 97 287 824 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 824 806 681 434 408
360 287 266 219 191 144 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 2419 2419
Size: 8443
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8443
SmaI CCC'GGG
Cuts at: 1872 3731 5537 5770 1872
Size: 1859 1806 233 4545
Fragments arranged by size:
4545 1859 1806 233
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3386 3932 5396 6547 7448 7710
Size: 197 926 1443 546 1464 1151 901 262
Cuts at: 7710 820
Size: 1553
Fragments arranged by size:
1553 1464 1443 1151 926 901 546 262
197
SnaBI TAC'GTA
Cuts at: 493 6234 493
Size: 5741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8443
SphI G_CATG'C
Cuts at: 1132 5545 5801 1132
Size: 4413 256 3774
Fragments arranged by size:
4413 3774 256
SspI AAT'ATT
Cuts at: 5 52 4436 6367 5
Size: 47 4384 1931 2081
Fragments arranged by size:
4384 2081 1931 47
StuI AGG'CCT
Cuts at: 1517 3182 3752 3759 1517
Size: 1665 570 7 6201
Fragments arranged by size:
6201 1665 570 7
StyI C'CwwG_G
Cuts at: 513 1065 2137 4002 4794 5882 513
Size: 552 1072 1865 792 1088 3074
Fragments arranged by size:
3074 1865 1088 1072 792 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2340 2654 3890 4944 5660 5753 6470
Size: 1255 760 314 1236 1054 716 93 717
Cuts at: 6470 6487 6640 6825 7270 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1236 1054 760 717 716 445
314 185 153 93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3163 3225 5047
Size: 274 80 33 51 1443 1186 62 1822
Cuts at: 5047 6689 8401 96
Size: 1642 1712 138
Fragments arranged by size:
1822 1712 1642 1443 1186 274 138 80
62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 4046 4078 4521 4553 5649 5681 8403
Size: 32 772 32 443 32 1096 32 2722
Cuts at: 8403 8435 3242
Size: 32 3250
Fragments arranged by size:
3250 2722 1096 772 443 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2984 4955 1316
Size: 1668 1971 4804
Fragments arranged by size:
4804 1971 1668
XbaI T'CTAG_A
Cuts at: 5779 5779
Size: 8443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2919 2965 5456 2919
Size: 46 2491 5906
Fragments arranged by size:
5906 2491 46
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8443
XmaI C'CCGG_G
Cuts at: 1870 3729 5535 5768 1870
Size: 1859 1806 233 4545
Fragments arranged by size:
4545 1859 1806 233
XmnI GAAnn'nnTTC
Cuts at: 6572 6572
Size: 8443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6251 276
Size: 53 83 186 936 4717 2468
Fragments arranged by size:
4717 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SfoI SrfI SwaI