Restriction map for
pFN21AE4595
(Circular) (Six-base) MAPSORT of: pFN21AE4595.seq Check: 8697 from: 1 to: 8428
pFN21AE4595 8428 bp
With 182 enzymes: SgfI *
March 28, 2012 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2298 3949 2298
Size: 1651 6777
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6238 278
Size: 53 83 186 936 4702 2468
Fragments arranged by size:
4702 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5011 5771 1937
Size: 3074 760 4594
Fragments arranged by size:
4594 3074 760
Acc65I G'GTAC_C
Cuts at: 2250 5749 2250
Size: 3499 4929
AclI AA'CG_TT
Cuts at: 1563 6555 6928 1563
Size: 4992 373 3063
Fragments arranged by size:
4992 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2861 3247 5151 5160 5422 5643 6491
Size: 447 782 386 1904 9 262 221 848
Cuts at: 6491 7875 8143 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1904 1384 848 782 447 386 268
262 221 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8231
AflIII A'CryG_T
Cuts at: 1203 3251 4728 4760 7327 8304 1203
Size: 2048 1477 32 2567 977 1327
Fragments arranged by size:
2567 2048 1477 1327 977 32
AgeI A'CCGG_T
Cuts at: 7259 7259
Size: 8428
AhdI GACnn_n'nnGTC
Cuts at: 2732 7157 2732
Size: 4425 4003
AleI CACnn'nnGTG
Cuts at: 1166 2833 4253 4593 4692 1166
Size: 1667 1420 340 99 4902
Fragments arranged by size:
4902 1667 1420 340 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2204 3020 1184
Size: 1020 816 6592
Fragments arranged by size:
6592 1020 816
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2558 2590 2558
Size: 32 8396
AlwNI CAG_nnn'CTG
Cuts at: 2510 2647 2839 3148 3334 3964 4363 5037 6171
Size: 137 192 309 186 630 399 674 1134
Cuts at: 6171 7743 2510
Size: 1572 3195
Fragments arranged by size:
3195 1572 1134 674 630 399 309 192
186 137
ApaI G_GGCC'C
Cuts at: 2113 2222 2364 2113
Size: 109 142 8177
Fragments arranged by size:
8177 142 109
ApaLI G'TGCA_C
Cuts at: 4211 6485 7641 4211
Size: 2274 1156 4998
Fragments arranged by size:
4998 2274 1156
ApoI r'AATT_y
Cuts at: 1493 2282 5737 5963 6115 8224 1493
Size: 789 3455 226 152 2109 1697
Fragments arranged by size:
3455 2109 1697 789 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6982 160
Size: 872 5950 1606
Fragments arranged by size:
5950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8428
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2245 2363 2805 3381 3534 3679
Size: 73 154 148 118 442 576 153 145
Cuts at: 3679 4031 4312 4599 5176 5481 5753 1870
Size: 352 281 287 577 305 272 4545
Fragments arranged by size:
4545 577 576 442 352 305 287 281
272 154 153 148 145 118 73
BamHI G'GATC_C
Cuts at: 2232 5638 5758 6125 2232
Size: 3406 120 367 4535
Fragments arranged by size:
4535 3406 367 120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2438 4181 4401 4471
Size: 325 217 627 463 188 1743 220 70
Cuts at: 4471 5749 7204 618
Size: 1278 1455 1842
Fragments arranged by size:
1842 1743 1455 1278 627 463 325 220
217 188 70
BanII G_rGCy'C
Cuts at: 1391 2113 2222 2364 2370 2576 2630 3426 3796
Size: 722 109 142 6 206 54 796 370
Cuts at: 3796 3894 3911 4114 5387 5747 1391
Size: 98 17 203 1273 360 4072
Fragments arranged by size:
4072 1273 796 722 370 360 206 203
142 109 98 54 17 6
BbeI G_GCGC'C
Cuts at: 4405 4475 4405
Size: 70 8358
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2937 5148 5309 5631 928
Size: 971 1038 2211 161 322 3725
Fragments arranged by size:
3725 2211 1038 971 322 161
BbvCI CC'TCA_GC
Cuts at: 2312 5286 5711 2312
Size: 2974 425 5029
Fragments arranged by size:
5029 2974 425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6619 6653 1903
Size: 34 4682 34 3678
Fragments arranged by size:
4682 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6320 7530 6320
Size: 1210 7218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2343 4034 4427
Size: 107 122 71 631 605 671 1691 393
Cuts at: 4427 7039 136
Size: 2612 1525
Fragments arranged by size:
2612 1691 1525 671 631 605 393 122
107 71
BglII A'GATC_T
Cuts at: 8423 8423
Size: 8428
BlpI GC'TnA_GC
Cuts at: 2948 3602 5845 2948
Size: 654 2243 5531
Fragments arranged by size:
5531 2243 654
Bme1580I G_kGCm'C
Cuts at: 1790 2113 2222 2364 2766 2876 4184 4215 4237
Size: 323 109 142 402 110 1308 31 22
Cuts at: 4237 4600 5526 6489 7645 1790
Size: 363 926 963 1156 2573
Fragments arranged by size:
2573 1308 1156 963 926 402 363 323
142 110 109 31 22
BmgBI CAC'GTC
Cuts at: 1318 4210 1318
Size: 2892 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4336 4346 6159
Size: 416 53 83 399 24 2919 10 1813
Cuts at: 6159 7117 442
Size: 958 1753
Fragments arranged by size:
2919 1813 1753 958 416 399 83 53
24 10
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8428
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2405 2963 5311 2161
Size: 244 558 2348 5278
Fragments arranged by size:
5278 2348 558 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2696 2706 3785 4223 4461 4581 4901 4935
Size: 1457 10 1079 438 238 120 320 34
Cuts at: 4935 5151 5583 7088 1239
Size: 216 432 1505 2579
Fragments arranged by size:
2579 1505 1457 1079 438 432 320 238
216 120 34 10
Bpu10I CC'TnA_GC
Cuts at: 2024 2312 2821 5286 5711 2024
Size: 288 509 2465 425 4741
Fragments arranged by size:
4741 2465 509 425 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6553 7418 7716 6553
Size: 865 298 7265
Fragments arranged by size:
7265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3278 5120 7091 882
Size: 617 1779 1842 1971 2219
Fragments arranged by size:
2219 1971 1842 1779 617
BsaAI yAC'GTr
Cuts at: 493 1204 2473 2767 3585 6219 493
Size: 711 1269 294 818 2634 2702
Fragments arranged by size:
2702 2634 1269 818 711 294
BsaBI GATnn'nnATC
Cuts at: 3049 4299 8422 3049
Size: 1250 4123 3055
Fragments arranged by size:
4123 3055 1250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2660 3687 4402 4472
Size: 53 83 186 936 1127 1027 715 70
Cuts at: 4472 5278 6235 6617 7252 275
Size: 806 957 382 635 1451
Fragments arranged by size:
1451 1127 1027 957 936 806 715 635
382 186 83 70 53
BsaWI w'CCGG_w
Cuts at: 6861 7259 7533 7680 8091 6861
Size: 398 274 147 411 7198
Fragments arranged by size:
7198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 2558 2588 2780
Size: 30 165 30 915 30 210 30 192
Cuts at: 2780 2810 4476 4506 5342 5372 5456 5486 1178
Size: 30 1666 30 836 30 84 30 4120
Fragments arranged by size:
4120 1666 915 836 210 192 165 84
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3173 4364 5216 5363 5480 1186
Size: 1987 1191 852 147 117 4134
Fragments arranged by size:
4134 1987 1191 852 147 117
BseYI C'CCAG_C
Cuts at: 2459 2577 2668 3130 3374 5091 5445 7631 8127
Size: 118 91 462 244 1717 354 2186 496
Cuts at: 8127 2459
Size: 2760
Fragments arranged by size:
2760 2186 1717 496 462 354 244 118
91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 3647 4145 4270 5612 1163
Size: 573 540 1371 498 125 1342 3979
Fragments arranged by size:
3979 1371 1342 573 540 498 125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2347 5878 6639 7667 1540
Size: 275 186 346 3531 761 1028 2301
Fragments arranged by size:
3531 2301 1028 761 346 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2630 2883 2994 3527 3894 3911 4215 4530
Size: 260 253 111 533 367 17 304 315
Cuts at: 4530 4680 5387 6489 6574 7645 2370
Size: 150 707 1102 85 1071 3153
Fragments arranged by size:
3153 1102 1071 707 533 367 315 304
260 253 150 111 85 17
BsmI GAATG_Cn'
Cuts at: 5939 6032 5939
Size: 93 8335
BsmBI CGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 8428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2222 2364 2370 2576 2630 2766
Size: 399 323 109 142 6 206 54 136
Cuts at: 2766 2876 2883 2994 3426 3527 3796 3894 3911
Size: 110 7 111 432 101 269 98 17
Cuts at: 3911 4114 4184 4215 4237 4530 4600 4680 5387
Size: 203 70 31 22 293 70 80 707
Cuts at: 5387 5526 5747 6489 6574 7645 1391
Size: 139 221 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 707 432 399 323 293
269 221 206 203 142 139 136 111
110 109 101 98 85 80 70 70
54 31 22 17 7 6
BspEI T'CCGG_A
Cuts at: 8091 8091
Size: 8428
BspHI T'CATG_A
Cuts at: 6317 8047 6317
Size: 1730 6698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2998 3878 3949 5783 844
Size: 1454 700 880 71 1834 3489
Fragments arranged by size:
3489 1834 1454 880 700 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2349 5371 5424 6315 1394
Size: 429 526 3022 53 891 3507
Fragments arranged by size:
3507 3022 891 526 429 53
BsrDI GCAATG_nn'
Cuts at: 65 1248 2198 4719 6923 7097 65
Size: 1183 950 2521 2204 174 1396
Fragments arranged by size:
2521 2204 1396 1183 950 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2854 3727 7072 7259 1700
Size: 110 1044 873 3345 187 2869
Fragments arranged by size:
3345 2869 1044 873 187 110
BsrGI T'GTAC_A
Cuts at: 96 4763 96
Size: 4667 3761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8428
BssSI C'ACGA_G
Cuts at: 2369 6488 7500 2369
Size: 4119 1012 3297
Fragments arranged by size:
4119 3297 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3148 794
Size: 2354 6074
BstBI TT'CG_AA
Cuts at: 7232 7232
Size: 8428
BstEII G'GTnAC_C
Cuts at: 1184 2446 3010 3970 1184
Size: 1262 564 960 5642
Fragments arranged by size:
5642 1262 960 564
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3619 4694 5129 2011
Size: 1608 1075 435 5310
Fragments arranged by size:
5310 1608 1075 435
BstYI r'GATC_y
Cuts at: 1972 2232 3044 4651 4660 5638 5758 6125 6510
Size: 260 812 1607 9 978 120 367 385
Cuts at: 6510 6527 7979 8065 8077 8423 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1607 1452 978 812 385 367 346
260 120 86 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 5429 5429
Size: 8428
BtgI C'CryG_G
Cuts at: 513 1065 2004 2442 2935 3107 3153 3975 4054
Size: 552 939 438 493 172 46 822 79
Cuts at: 4054 4185 4872 5388 513
Size: 131 687 516 3553
Fragments arranged by size:
3553 939 822 687 552 516 493 438
172 131 79 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2331 4076 487
Size: 683 56 1105 1745 4839
Fragments arranged by size:
4839 1745 1105 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 2828 2840 2908 3002 3671 4162 4588 5944
Size: 640 12 68 94 669 491 426 1356
Cuts at: 5944 6738 6758 2188
Size: 794 20 3858
Fragments arranged by size:
3858 1356 794 669 640 491 426 94
68 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8393
DraI TTT'AAA
Cuts at: 5732 5732
Size: 8428
DraIII CAC_nnn'GTG
Cuts at: 2868 4873 4966 2868
Size: 2005 93 6330
Fragments arranged by size:
6330 2005 93
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5277 7435 809
Size: 507 3961 2158 1802
Fragments arranged by size:
3961 2158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2344 2744 2848
Size: 54 1425 50 275 18 514 400 104
Cuts at: 2848 3657 3774 3856 5046 5664 5875 6764 8393
Size: 809 117 82 1190 618 211 889 1629
Cuts at: 8393 8
Size: 43
Fragments arranged by size:
1629 1425 1190 889 809 618 514 400
275 211 117 104 82 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2344 5875 1537
Size: 275 532 3531 4090
Fragments arranged by size:
4090 3531 532 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 4760 5316 6358 1353
Size: 1241 2166 556 1042 3423
Fragments arranged by size:
3423 2166 1241 1042 556
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3714 3799 7015 7389 7535 387
Size: 1064 138 2125 85 3216 374 146 1280
Fragments arranged by size:
3216 2125 1280 1064 374 146 138 85
EcoICRI GAG'CTC
Cuts at: 2368 2628 3892 3909 5385 2368
Size: 260 1264 17 1476 5411
Fragments arranged by size:
5411 1476 1264 260 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2696 2706 2861 3247 3785 4223
Size: 393 447 617 10 155 386 538 438
Cuts at: 4223 4461 4581 4901 4935 5151 5160 5422 5583
Size: 238 120 320 34 216 9 262 161
Cuts at: 5583 5643 6491 7088 7875 8143 1239
Size: 60 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 617 597 538 447 438
393 386 320 268 262 238 216 161
155 120 60 34 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2274 2756 4544 4836 2274
Size: 482 1788 292 5866
Fragments arranged by size:
5866 1788 482 292
EcoO109I rG'GnC_Cy
Cuts at: 2110 2142 2219 2266 2360 2680 2726 2982 3347
Size: 32 77 47 94 320 46 256 365
Cuts at: 3347 3378 4243 4642 4906 4939 5088 5171 5254
Size: 31 865 399 264 33 149 83 83
Cuts at: 5254 5573 5600 5608 2110
Size: 319 27 8 4930
Fragments arranged by size:
4930 865 399 365 320 319 264 256
149 94 83 83 77 47 46 33
32 31 27 8
EcoRI G'AATT_C
Cuts at: 2282 5737 6115 2282
Size: 3455 378 4595
Fragments arranged by size:
4595 3455 378
EcoRV GAT'ATC
Cuts at: 4383 4383
Size: 8428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 4912 3304
Size: 1608 6820
FspI TGC'GCA
Cuts at: 6934 6934
Size: 8428
HaeII r_GCGC'y
Cuts at: 1678 3888 4405 4443 4475 7575 1678
Size: 2210 517 38 32 3100 2531
Fragments arranged by size:
3100 2531 2210 517 38 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 2718 2750 3331
Size: 32 135 32 785 32 329 32 581
Cuts at: 3331 3363 4367 4399 4476 4508 4820 4852 5019
Size: 32 1004 32 77 32 312 32 167
Cuts at: 5019 5051 5300 5332 5456 5488 5704 5736 7069
Size: 32 249 32 124 32 216 32 1333
Cuts at: 7069 7101 7143 7175 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1333 1004 785 581 329 312 249
216 167 135 124 77 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 3733 5772 6018 669
Size: 988 281 541 1254 2039 246 3079
Fragments arranged by size:
3079 2039 1254 988 541 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8428
HpaI GTT'AAC
Cuts at: 6018 6018
Size: 8428
KasI G'GCGC_C
Cuts at: 4401 4471 4401
Size: 70 8358
KpnI G_GTAC'C
Cuts at: 2254 5753 2254
Size: 3499 4929
MfeI C'AATT_G
Cuts at: 6027 6027
Size: 8428
MluI A'CGCG_T
Cuts at: 8304 8304
Size: 8428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3228 3543 5298 7542 7726 1305
Size: 1923 315 1755 2244 184 2007
Fragments arranged by size:
2244 2007 1923 1755 315 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2746 2850 3659 3858 5048
Size: 54 1425 343 914 104 809 199 1190
Cuts at: 5048 10
Size: 3390
Fragments arranged by size:
3390 1425 1190 914 809 343 199 104
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2833 3197 4253 4593 4692 5529 6386
Size: 648 1667 364 1056 340 99 837 857
Cuts at: 6386 6745 6904 518
Size: 359 159 2042
Fragments arranged by size:
2042 1667 1056 857 837 648 364 359
340 159 99
MspA1I CmG'CkG
Cuts at: 1606 2952 3109 3145 3619 3977 4056 4076 4107
Size: 1346 157 36 474 358 79 20 31
Cuts at: 4107 4159 4187 4559 5149 5266 5449 5844 6521
Size: 52 28 372 590 117 183 395 677
Cuts at: 6521 7669 7914 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1346 1148 677 590 474 395 372
358 245 183 157 117 79 52 36
31 28 20
NarI GG'CG_CC
Cuts at: 4402 4472 4402
Size: 70 8358
NcoI C'CATG_G
Cuts at: 513 1065 2004 2442 2935 3153 513
Size: 552 939 438 493 218 5788
Fragments arranged by size:
5788 939 552 493 438 218
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8428
NotI GC'GGCC_GC
Cuts at: 5875 5875
Size: 8428
NspI r_CATG'y
Cuts at: 1132 1229 3255 3705 4321 4764 5786 7331 1132
Size: 97 2026 450 616 443 1022 1545 2229
Fragments arranged by size:
2229 2026 1545 1022 616 450 443 97
PasI CC'CwG_GG
Cuts at: 1348 2011 4942 5519 5604 1348
Size: 663 2931 577 85 4172
Fragments arranged by size:
4172 2931 663 577 85
PciI A'CATG_T
Cuts at: 3251 4760 7327 3251
Size: 1509 2567 4352
Fragments arranged by size:
4352 2567 1509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2868 2893 4363 4966 1108
Size: 1760 25 1470 603 4570
Fragments arranged by size:
4570 1760 1470 603 25
PfoI T'CCnGG_A
Cuts at: 2722 4239 2722
Size: 1517 6911
PmeI GTTT'AAAC
Cuts at: 5732 5732
Size: 8428
PmlI CAC'GTG
Cuts at: 2767 3585 2767
Size: 818 7610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2558 2590 6497 6529 8010 8042 2558
Size: 32 3907 32 1481 32 2944
Fragments arranged by size:
3907 2944 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2726 2982 3347 3378 4243 4642 5088 5171
Size: 460 256 365 31 865 399 446 83
Cuts at: 5171 5254 5573 5600 5608 2266
Size: 83 319 27 8 5086
Fragments arranged by size:
5086 865 460 446 399 365 319 256
83 83 31 27 8
PshAI GACnn'nnGTC
Cuts at: 1613 3237 3704 1613
Size: 1624 467 6337
Fragments arranged by size:
6337 1624 467
PsiI TTA'TAA
Cuts at: 5998 5998
Size: 8428
PspOMI G'GGCC_C
Cuts at: 2109 2218 2360 2109
Size: 109 142 8177
Fragments arranged by size:
8177 142 109
PstI C_TGCA'G
Cuts at: 830 2835 2980 3009 4558 5780 830
Size: 2005 145 29 1549 1222 3478
Fragments arranged by size:
3478 2005 1549 1222 145 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8428
PvuII CAG'CTG
Cuts at: 2952 3145 3619 4159 4559 5149 5449 2952
Size: 193 474 540 400 590 300 5931
Fragments arranged by size:
5931 590 540 474 400 300 193
SacI G_AGCT'C
Cuts at: 2370 2630 3894 3911 5387 2370
Size: 260 1264 17 1476 5411
Fragments arranged by size:
5411 1476 1264 260 17
SacII CC_GC'GG
Cuts at: 3110 3978 4057 4188 3110
Size: 868 79 131 7350
Fragments arranged by size:
7350 868 131 79
SalI G'TCGA_C
Cuts at: 1936 5770 1936
Size: 3834 4594
SanDI GG'GwC_CC
Cuts at: 2266 2982 3347 3378 4642 5088 5573 5600 2266
Size: 716 365 31 1264 446 485 27 5094
Fragments arranged by size:
5094 1264 716 485 446 365 31 27
SbfI CC_TGCA'GG
Cuts at: 5780 5780
Size: 8428
ScaI AGT'ACT
Cuts at: 6676 8388 6676
Size: 1712 6716
SexAI A'CCwGG_T
Cuts at: 5658 5658
Size: 8428
SfcI C'TryA_G
Cuts at: 826 1045 2831 2976 3005 4554 5012 5776 6911
Size: 219 1786 145 29 1549 458 764 1135
Cuts at: 6911 7592 7783 826
Size: 681 191 1471
Fragments arranged by size:
1786 1549 1471 1135 764 681 458 219
191 145 29
SfoI GGC'GCC
Cuts at: 4403 4473 4403
Size: 70 8358
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8428
SmaI CCC'GGG
Cuts at: 1872 2099 3383 3681 4314 5755 1872
Size: 227 1284 298 633 1441 4545
Fragments arranged by size:
4545 1441 1284 633 298 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6532 7433 7695 820
Size: 197 926 4589 901 262 1553
Fragments arranged by size:
4589 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6219 493
Size: 5726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8428
SphI G_CATG'C
Cuts at: 1132 5786 1132
Size: 4654 3774
SspI AAT'ATT
Cuts at: 5 52 6352 5
Size: 47 6300 2081
Fragments arranged by size:
6300 2081 47
StuI AGG'CCT
Cuts at: 1517 2064 2303 2693 2751 3260 4037 1517
Size: 547 239 390 58 509 777 5908
Fragments arranged by size:
5908 777 547 509 390 239 58
StyI C'CwwG_G
Cuts at: 513 1065 2004 2442 2935 3153 3279 4780 5058
Size: 552 939 438 493 218 126 1501 278
Cuts at: 5058 5867 513
Size: 809 3074
Fragments arranged by size:
3074 1501 939 809 552 493 438 278
218 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2900 3476 5343 6455 6472 6625 6810
Size: 1255 1320 576 1867 1112 17 153 185
Cuts at: 6810 7255 325
Size: 445 1498
Fragments arranged by size:
1867 1498 1320 1255 1112 576 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4763 6674 8386
Size: 274 80 33 51 1443 2786 1911 1712
Cuts at: 8386 96
Size: 138
Fragments arranged by size:
2786 1911 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 2780 2812 5342 5374 5709 5741 8388
Size: 32 430 32 2530 32 335 32 2647
Cuts at: 8388 8420 2318
Size: 32 2326
Fragments arranged by size:
2647 2530 2326 430 335 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3463 4280 5330 1316
Size: 2147 817 1050 4414
Fragments arranged by size:
4414 2147 1050 817
XbaI T'CTAG_A
Cuts at: 5764 5764
Size: 8428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3037 5056 5126 3037
Size: 2019 70 6339
Fragments arranged by size:
6339 2019 70
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8428
XmaI C'CCGG_G
Cuts at: 1870 2097 3381 3679 4312 5753 1870
Size: 227 1284 298 633 1441 4545
Fragments arranged by size:
4545 1441 1284 633 298 227
XmnI GAAnn'nnTTC
Cuts at: 2588 6557 2588
Size: 3969 4459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6236 276
Size: 53 83 186 936 4702 2468
Fragments arranged by size:
4702 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SapI SfiI SrfI SwaI