Restriction map for
pFN21AE4594
(Circular) (Six-base) MAPSORT of: pFN21AE4594.seq Check: 7282 from: 1 to: 8410
pFN21AE4594 8410 bp
With 182 enzymes: SgfI *
March 28, 2012 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3668 3668
Size: 8410
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6220 278
Size: 53 83 186 936 4684 2468
Fragments arranged by size:
4684 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 3174 5753 1937
Size: 816 421 2579 4594
Fragments arranged by size:
4594 2579 816 421
Acc65I G'GTAC_C
Cuts at: 5731 5731
Size: 8410
AclI AA'CG_TT
Cuts at: 1563 6537 6910 1563
Size: 4974 373 3063
Fragments arranged by size:
4974 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3010 3285 4057 6473 7857 8125 1632
Size: 1378 275 772 2416 1384 268 1917
Fragments arranged by size:
2416 1917 1384 1378 772 275 268
AfeI AGC'GCT
Cuts at: 5614 5614
Size: 8410
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8213
AflIII A'CryG_T
Cuts at: 1203 7309 8286 1203
Size: 6106 977 1327
Fragments arranged by size:
6106 1327 977
AgeI A'CCGG_T
Cuts at: 7241 7241
Size: 8410
AhdI GACnn_n'nnGTC
Cuts at: 3912 5542 7139 3912
Size: 1630 1597 5183
Fragments arranged by size:
5183 1630 1597
AleI CACnn'nnGTG
Cuts at: 1166 2375 1166
Size: 1209 7201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2142 4550 1184
Size: 958 2408 5044
Fragments arranged by size:
5044 2408 958
AlwNI CAG_nnn'CTG
Cuts at: 5206 5422 6153 7725 5206
Size: 216 731 1572 5891
Fragments arranged by size:
5891 1572 731 216
ApaLI G'TGCA_C
Cuts at: 3655 6467 7623 3655
Size: 2812 1156 4442
Fragments arranged by size:
4442 2812 1156
ApoI r'AATT_y
Cuts at: 1493 3481 3821 5719 5945 6097 8206 1493
Size: 1988 340 1898 226 152 2109 1697
Fragments arranged by size:
2109 1988 1898 1697 340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6964 160
Size: 872 5932 1606
Fragments arranged by size:
5932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8410
AvaI C'yCGr_G
Cuts at: 1870 1943 4574 4581 5161 5334 5405 5735 1870
Size: 73 2631 7 580 173 71 330 4545
Fragments arranged by size:
4545 2631 580 330 173 73 71 7
BamHI G'GATC_C
Cuts at: 3099 3862 5740 6107 3099
Size: 763 1878 367 5402
Fragments arranged by size:
5402 1878 763 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3109 3196 5552 5731 7186
Size: 325 217 627 1322 87 2356 179 1455
Cuts at: 7186 618
Size: 1842
Fragments arranged by size:
2356 1842 1455 1322 627 325 217 179
87
BanII G_rGCy'C
Cuts at: 1391 2665 4434 4581 4623 5729 1391
Size: 1274 1769 147 42 1106 4072
Fragments arranged by size:
4072 1769 1274 1106 147 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 3016 3957 4063 4065 928
Size: 971 359 758 941 106 2 5273
Fragments arranged by size:
5273 971 941 758 359 106 2
BbvCI CC'TCA_GC
Cuts at: 3230 4212 4518 4841 3230
Size: 982 306 323 6799
Fragments arranged by size:
6799 982 323 306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6601 6635 1903
Size: 34 50 34 4580 34 3678
Fragments arranged by size:
4580 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2165 6302 7512 2165
Size: 4137 1210 3063
Fragments arranged by size:
4137 3063 1210
BclI T'GATC_A
Cuts at: 1548 4761 1548
Size: 3213 5197
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7021 136
Size: 107 122 71 631 605 5349 1525
Fragments arranged by size:
5349 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8405 8405
Size: 8410
BlpI GC'TnA_GC
Cuts at: 5827 5827
Size: 8410
Bme1580I G_kGCm'C
Cuts at: 1790 3659 4180 6471 7627 1790
Size: 1869 521 2291 1156 2573
Fragments arranged by size:
2573 2291 1869 1156 521
BmgBI CAC'GTC
Cuts at: 1318 4276 5148 1318
Size: 2958 872 4580
Fragments arranged by size:
4580 2958 872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3565 6141 7099
Size: 416 53 83 399 24 2148 2576 958
Cuts at: 7099 442
Size: 1753
Fragments arranged by size:
2576 2148 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8410
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2285 2285
Size: 8410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2387 2849 3089 3685 5264 5588 7070
Size: 1016 132 462 240 596 1579 324 1482
Cuts at: 7070 1239
Size: 2579
Fragments arranged by size:
2579 1579 1482 1016 596 462 324 240
132
Bpu10I CC'TnA_GC
Cuts at: 3230 3632 4212 4518 4807 4841 3230
Size: 402 580 306 289 34 6799
Fragments arranged by size:
6799 580 402 306 289 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2942 6535 7400 7698 2651
Size: 291 3593 865 298 3363
Fragments arranged by size:
3593 3363 865 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5300 7073 882
Size: 617 3801 1773 2219
Fragments arranged by size:
3801 2219 1773 617
BsaAI yAC'GTr
Cuts at: 493 1204 2465 5494 6201 493
Size: 711 1261 3029 707 2702
Fragments arranged by size:
3029 2702 1261 711 707
BsaBI GATnn'nnATC
Cuts at: 8404 8404
Size: 8410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6217 6599 7234 275
Size: 53 83 186 936 4684 382 635 1451
Fragments arranged by size:
4684 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4749 4973 6843 7241 7515 7662 8073 4749
Size: 224 1870 398 274 147 411 5086
Fragments arranged by size:
5086 1870 411 398 274 224 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3783 3813 5282 5312 1178
Size: 30 165 30 2380 30 1469 30 4276
Fragments arranged by size:
4276 2380 1469 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3804 4029 4200 4631 4918 5008 5029 1186
Size: 2618 225 171 431 287 90 21 4567
Fragments arranged by size:
4567 2618 431 287 225 171 90 21
BseYI C'CCAG_C
Cuts at: 4370 4374 4721 5181 7613 8109 4370
Size: 4 347 460 2432 496 4671
Fragments arranged by size:
4671 2432 496 460 347 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 2324 4510 4853 5528 1163
Size: 573 342 246 2186 343 675 4045
Fragments arranged by size:
4045 2186 675 573 343 342 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3497 5860 6621 7649 1540
Size: 275 186 1496 2363 761 1028 2301
Fragments arranged by size:
2363 2301 1496 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2327 2715 3659 3753 4434 4623 6471 6556 7627
Size: 388 944 94 681 189 1848 85 1071
Cuts at: 7627 2327
Size: 3110
Fragments arranged by size:
3110 1848 1071 944 681 388 189 94
85
BsmI GAATG_Cn'
Cuts at: 2200 2602 5098 5921 6014 2200
Size: 402 2496 823 93 4596
Fragments arranged by size:
4596 2496 823 402 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2327 2665 2715 3659 3753 4180 4434
Size: 399 537 338 50 944 94 427 254
Cuts at: 4434 4581 4623 5729 6471 6556 7627 1391
Size: 147 42 1106 742 85 1071 2174
Fragments arranged by size:
2174 1106 1071 944 742 537 427 399
338 254 147 94 85 50 42
BspEI T'CCGG_A
Cuts at: 4749 8073 4749
Size: 3324 5086
BspHI T'CATG_A
Cuts at: 6299 8029 6299
Size: 1730 6680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3668 4542 5765 844
Size: 2824 874 1223 3489
Fragments arranged by size:
3489 2824 1223 874
BsrBI CCG'CTC
Cuts at: 1394 1823 5191 6297 1394
Size: 429 3368 1106 3507
Fragments arranged by size:
3507 3368 1106 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4662 6905 7079 65
Size: 1183 3414 2243 174 1396
Fragments arranged by size:
3414 2243 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4445 5412 7054 7241 1700
Size: 110 2635 967 1642 187 2869
Fragments arranged by size:
2869 2635 1642 967 187 110
BsrGI T'GTAC_A
Cuts at: 96 3222 4344 96
Size: 3126 1122 4162
Fragments arranged by size:
4162 3126 1122
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8410
BssSI C'ACGA_G
Cuts at: 4958 6470 7482 4958
Size: 1512 1012 5886
Fragments arranged by size:
5886 1512 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8410
BstBI TT'CG_AA
Cuts at: 2967 4316 7214 2967
Size: 1349 2898 4163
Fragments arranged by size:
4163 2898 1349
BstEII G'GTnAC_C
Cuts at: 1184 4405 1184
Size: 3221 5189
BstXI CCAn_nnnn'nTGG
Cuts at: 4786 4786
Size: 8410
BstYI r'GATC_y
Cuts at: 1972 3099 3862 5274 5740 6107 6492 6509 7961
Size: 1127 763 1412 466 367 385 17 1452
Cuts at: 7961 8047 8059 8405 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1412 1127 763 466 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2455 2455
Size: 8410
BtgI C'CryG_G
Cuts at: 513 1065 2665 4560 4779 5247 5290 5556 513
Size: 552 1600 1895 219 468 43 266 3367
Fragments arranged by size:
3367 1895 1600 552 468 266 219 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2220 487
Size: 683 56 994 6677
Fragments arranged by size:
6677 994 683 56
BtsI GCAGTG_nn'
Cuts at: 2713 2817 5098 5664 5926 6720 6740 2713
Size: 104 2281 566 262 794 20 4383
Fragments arranged by size:
4383 2281 794 566 262 104 20
ClaI AT'CG_AT
Cuts at: 1554 3273 5304 1554
Size: 1719 2031 4660
Fragments arranged by size:
4660 2031 1719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5640 5675 529
Size: 35 5076 35 3264
Fragments arranged by size:
5076 3264 35 35
DraI TTT'AAA
Cuts at: 5714 5714
Size: 8410
DraIII CAC_nnn'GTG
Cuts at: 3382 4963 3382
Size: 1581 6829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4067 7417 809
Size: 507 2751 3350 1802
Fragments arranged by size:
3350 2751 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2292 2616
Size: 54 1425 50 275 18 330 132 324
Cuts at: 2616 4698 4776 5514 5857 6746 8375 8
Size: 2082 78 738 343 889 1629 43
Fragments arranged by size:
2082 1629 1425 889 738 343 330 324
275 132 78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5857 1537
Size: 275 4045 4090
Fragments arranged by size:
4090 4045 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2429 2969 3144 4809 6340 1353
Size: 1076 540 175 1665 1531 3423
Fragments arranged by size:
3423 1665 1531 1076 540 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3092 6997 7371 7517 387
Size: 1064 138 1503 3905 374 146 1280
Fragments arranged by size:
3905 1503 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4432 4621 4432
Size: 189 8221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2387 2849 3010 3089 3285 3685
Size: 393 623 132 462 161 79 196 400
Cuts at: 3685 4057 5264 5588 6473 7070 7857 8125 1239
Size: 372 1207 324 885 597 787 268 1524
Fragments arranged by size:
1524 1207 885 787 623 597 462 400
393 372 324 268 196 161 132 79
EcoNI CCTnn'n_nnAGG
Cuts at: 3664 4226 4517 4676 3664
Size: 562 291 159 7398
Fragments arranged by size:
7398 562 291 159
EcoO109I rG'GnC_Cy
Cuts at: 2170 2923 3064 4026 4367 4510 5242 2170
Size: 753 141 962 341 143 732 5338
Fragments arranged by size:
5338 962 753 732 341 143 141
EcoRI G'AATT_C
Cuts at: 5719 6097 5719
Size: 378 8032
EcoRV GAT'ATC
Cuts at: 3944 3944
Size: 8410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3998 3998
Size: 8410
FspI TGC'GCA
Cuts at: 5097 5210 6916 5097
Size: 113 1706 6591
Fragments arranged by size:
6591 1706 113
FspAI rTGC'GCAy
Cuts at: 5097 5097
Size: 8410
HaeII r_GCGC'y
Cuts at: 1678 5616 7557 1678
Size: 3938 1941 2531
Fragments arranged by size:
3938 2531 1941
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 2402 2434 3427
Size: 32 135 32 676 32 122 32 993
Cuts at: 3427 3459 3672 3704 3898 3930 4015 4047 5160
Size: 32 213 32 194 32 85 32 1113
Cuts at: 5160 5192 5503 5528 5535 5560 7051 7083 7125
Size: 32 311 25 7 25 1491 32 42
Cuts at: 7125 7157 1373
Size: 32 2626
Fragments arranged by size:
2626 1491 1113 993 676 311 213 194
135 122 85 42 32 32 32 32
32 32 32 32 32 32 32 25
25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 5754 6000 669
Size: 988 281 816 3000 246 3079
Fragments arranged by size:
3079 3000 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 3458 748
Size: 2710 5700
HpaI GTT'AAC
Cuts at: 6000 6000
Size: 8410
KpnI G_GTAC'C
Cuts at: 5735 5735
Size: 8410
MfeI C'AATT_G
Cuts at: 2637 5547 6009 2637
Size: 2910 462 5038
Fragments arranged by size:
5038 2910 462
MluI A'CGCG_T
Cuts at: 8286 8286
Size: 8410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7524 7708 1305
Size: 6219 184 2007
Fragments arranged by size:
6219 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 2618 4700 4778 5516
Size: 54 1425 343 462 324 2082 78 738
Cuts at: 5516 10
Size: 2904
Fragments arranged by size:
2904 2082 1425 738 462 343 324 78
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 3450 3510 4769 5438 5465 5492
Size: 648 1209 1075 60 1259 669 27 27
Cuts at: 5492 6368 6727 6886 518
Size: 876 359 159 2042
Fragments arranged by size:
2042 1259 1209 1075 876 669 648 359
159 60 27 27
MspA1I CmG'CkG
Cuts at: 1606 3263 4374 5419 5524 5826 6503 7651 7896
Size: 1657 1111 1045 105 302 677 1148 245
Cuts at: 7896 1606
Size: 2120
Fragments arranged by size:
2120 1657 1148 1111 1045 677 302 245
105
NaeI GCC'GGC
Cuts at: 4447 4447
Size: 8410
NcoI C'CATG_G
Cuts at: 513 1065 2665 5556 513
Size: 552 1600 2891 3367
Fragments arranged by size:
3367 2891 1600 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8410
NgoMIV G'CCGG_C
Cuts at: 4445 4445
Size: 8410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8410
NotI GC'GGCC_GC
Cuts at: 5857 5857
Size: 8410
NspI r_CATG'y
Cuts at: 1132 1229 5768 7313 1132
Size: 97 4539 1545 2229
Fragments arranged by size:
4539 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8410
PciI A'CATG_T
Cuts at: 7309 7309
Size: 8410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4963 1108
Size: 3855 4555
PfoI T'CCnGG_A
Cuts at: 2586 2586
Size: 8410
PmeI GTTT'AAAC
Cuts at: 5714 5714
Size: 8410
PmlI CAC'GTG
Cuts at: 5494 5494
Size: 8410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2946 2978 6479 6511 7992 8024 2946
Size: 32 3501 32 1481 32 3332
Fragments arranged by size:
3501 3332 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2923 4026 4367 4510 5242 2923
Size: 1103 341 143 732 6091
Fragments arranged by size:
6091 1103 732 341 143
PshAI GACnn'nnGTC
Cuts at: 1613 2677 1613
Size: 1064 7346
PsiI TTA'TAA
Cuts at: 2907 3011 5980 2907
Size: 104 2969 5337
Fragments arranged by size:
5337 2969 104
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3165 3197 3165
Size: 32 8378
PstI C_TGCA'G
Cuts at: 830 5762 830
Size: 4932 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8410
PvuII CAG'CTG
Cuts at: 4374 5419 5524 4374
Size: 1045 105 7260
Fragments arranged by size:
7260 1045 105
SacI G_AGCT'C
Cuts at: 4434 4623 4434
Size: 189 8221
SalI G'TCGA_C
Cuts at: 1936 2752 5752 1936
Size: 816 3000 4594
Fragments arranged by size:
4594 3000 816
SanDI GG'GwC_CC
Cuts at: 4367 5242 4367
Size: 875 7535
SapI GCTCTTCn'nnn_
Cuts at: 2969 2969
Size: 8410
SbfI CC_TGCA'GG
Cuts at: 5762 5762
Size: 8410
ScaI AGT'ACT
Cuts at: 6658 8370 6658
Size: 1712 6698
SexAI A'CCwGG_T
Cuts at: 5538 5538
Size: 8410
SfcI C'TryA_G
Cuts at: 826 1045 2149 4322 5758 6893 7574 7765 826
Size: 219 1104 2173 1436 1135 681 191 1471
Fragments arranged by size:
2173 1471 1436 1135 1104 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8410
SmaI CCC'GGG
Cuts at: 1872 4583 5737 1872
Size: 2711 1154 4545
Fragments arranged by size:
4545 2711 1154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2630 2957 6514 7415 7677 820
Size: 197 926 687 327 3557 901 262 1553
Fragments arranged by size:
3557 1553 926 901 687 327 262 197
SnaBI TAC'GTA
Cuts at: 493 2465 6201 493
Size: 1972 3736 2702
Fragments arranged by size:
3736 2702 1972
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8410
SphI G_CATG'C
Cuts at: 1132 5768 1132
Size: 4636 3774
SspI AAT'ATT
Cuts at: 5 52 6334 5
Size: 47 6282 2081
Fragments arranged by size:
6282 2081 47
StuI AGG'CCT
Cuts at: 1517 2225 4488 4523 1517
Size: 708 2263 35 5404
Fragments arranged by size:
5404 2263 708 35
StyI C'CwwG_G
Cuts at: 513 1065 2220 2328 2619 2665 3517 5556 5849
Size: 552 1155 108 291 46 852 2039 293
Cuts at: 5849 513
Size: 3074
Fragments arranged by size:
3074 2039 1155 852 552 293 291 108
46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2122 3192 6437 6454 6607 6792 7237
Size: 1255 542 1070 3245 17 153 185 445
Cuts at: 7237 325
Size: 1498
Fragments arranged by size:
3245 1498 1255 1070 542 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 3153 3222
Size: 274 80 33 51 1443 372 804 69
Cuts at: 3222 3322 4344 6656 8368 96
Size: 100 1022 2312 1712 138
Fragments arranged by size:
2312 1712 1443 1022 804 372 274 138
100 80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3678 3710 5282 5314 8370 8402 3678
Size: 32 1572 32 3056 32 3686
Fragments arranged by size:
3686 3056 1572 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8410
XbaI T'CTAG_A
Cuts at: 5746 5746
Size: 8410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2873 4729 4880 4960 5546 5563 2873
Size: 1856 151 80 586 17 5720
Fragments arranged by size:
5720 1856 586 151 80 17
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8410
XmaI C'CCGG_G
Cuts at: 1870 4581 5735 1870
Size: 2711 1154 4545
Fragments arranged by size:
4545 2711 1154
XmnI GAAnn'nnTTC
Cuts at: 3292 4230 6539 3292
Size: 938 2309 5163
Fragments arranged by size:
5163 2309 938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6218 276
Size: 53 83 186 936 4684 2468
Fragments arranged by size:
4684 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I FseI KasI NarI NruI NsiI PacI
PspOMI PspXI RsrII SacII SfiI SfoI SrfI SwaI