Restriction map for
pFN21AE4590
(Circular) (Six-base) MAPSORT of: pFN21AE4590.seq Check: 5105 from: 1 to: 7987
pFN21AE4590 7987 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5797 278
Size: 53 83 186 936 4261 2468
Fragments arranged by size:
4261 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 5330 1937
Size: 939 2454 4594
Fragments arranged by size:
4594 2454 939
Acc65I G'GTAC_C
Cuts at: 3900 5308 3900
Size: 1408 6579
AclI AA'CG_TT
Cuts at: 1563 6114 6487 1563
Size: 4551 373 3063
Fragments arranged by size:
4551 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2343 2396 2912 3015 3140 3188 4281 4554
Size: 711 53 516 103 125 48 1093 273
Cuts at: 4554 5210 5285 6050 7434 7702 1632
Size: 656 75 765 1384 268 1917
Fragments arranged by size:
1917 1384 1093 765 711 656 516 273
268 125 103 75 53 48
AfeI AGC'GCT
Cuts at: 2407 2407
Size: 7987
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7790
AflIII A'CryG_T
Cuts at: 1203 3324 6886 7863 1203
Size: 2121 3562 977 1327
Fragments arranged by size:
3562 2121 1327 977
AgeI A'CCGG_T
Cuts at: 6818 6818
Size: 7987
AhdI GACnn_n'nnGTC
Cuts at: 6716 6716
Size: 7987
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2355 4808 1184
Size: 1171 2453 4363
Fragments arranged by size:
4363 2453 1171
AlwNI CAG_nnn'CTG
Cuts at: 4367 5730 7302 4367
Size: 1363 1572 5052
Fragments arranged by size:
5052 1572 1363
ApaLI G'TGCA_C
Cuts at: 5153 6044 7200 5153
Size: 891 1156 5940
Fragments arranged by size:
5940 1156 891
ApoI r'AATT_y
Cuts at: 1493 2818 3177 3227 4012 5050 5296 5522 5674
Size: 1325 359 50 785 1038 246 226 152
Cuts at: 5674 7783 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1325 1038 785 359 246 226
152 50
AseI AT'TA_AT
Cuts at: 160 1032 6541 160
Size: 872 5509 1606
Fragments arranged by size:
5509 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7987
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 5312 1870
Size: 73 152 3217 4545
Fragments arranged by size:
4545 3217 152 73
BamHI G'GATC_C
Cuts at: 2884 4832 5317 5684 2884
Size: 1948 485 367 5187
Fragments arranged by size:
5187 1948 485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3863 3900 3931 5308 6763
Size: 325 217 627 2076 37 31 1377 1455
Cuts at: 6763 618
Size: 1842
Fragments arranged by size:
2076 1842 1455 1377 627 325 217 37
31
BanII G_rGCy'C
Cuts at: 1391 2374 5306 1391
Size: 983 2932 4072
Fragments arranged by size:
4072 2932 983
BbeI G_GCGC'C
Cuts at: 3935 3935
Size: 7987
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2811 4338 928
Size: 971 912 1527 4577
Fragments arranged by size:
4577 1527 971 912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6178 6212 1903
Size: 34 4241 34 3678
Fragments arranged by size:
4241 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4495 5879 7089 4495
Size: 1384 1210 5393
Fragments arranged by size:
5393 1384 1210
BclI T'GATC_A
Cuts at: 1548 2839 3156 3697 1548
Size: 1291 317 541 5838
Fragments arranged by size:
5838 1291 541 317
BfrBI ATG'CAT
Cuts at: 2231 4795 2231
Size: 2564 5423
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3336 6598 136
Size: 107 122 71 631 605 1664 3262 1525
Fragments arranged by size:
3262 1664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2140 2662 4462 7982 2140
Size: 522 1800 3520 2145
Fragments arranged by size:
3520 2145 1800 522
BlpI GC'TnA_GC
Cuts at: 2964 5404 2964
Size: 2440 5547
Bme1580I G_kGCm'C
Cuts at: 1790 5157 6048 7204 1790
Size: 3367 891 1156 2573
Fragments arranged by size:
3367 2573 1156 891
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7987
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3664 3906 4475
Size: 416 53 83 399 24 2247 242 569
Cuts at: 4475 5718 6676 442
Size: 1243 958 1753
Fragments arranged by size:
2247 1753 1243 958 569 416 399 242
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7987
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 4072 4180 2095
Size: 1977 108 5902
Fragments arranged by size:
5902 1977 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4150 4476 6647 1239
Size: 2911 326 2171 2579
Fragments arranged by size:
2911 2579 2171 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3845 4841 6112 6977 7275 3845
Size: 996 1271 865 298 4557
Fragments arranged by size:
4557 1271 996 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3711 4708 6650 882
Size: 617 2212 997 1942 2219
Fragments arranged by size:
2219 2212 1942 997 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 4618 5778 493
Size: 711 2123 1291 1160 2702
Fragments arranged by size:
2702 2123 1291 1160 711
BsaBI GATnn'nnATC
Cuts at: 2838 2889 3846 7981 2838
Size: 51 957 4135 2844
Fragments arranged by size:
4135 2844 957 51
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3932 5794 6176 6811
Size: 53 83 186 936 2399 1862 382 635
Cuts at: 6811 275
Size: 1451
Fragments arranged by size:
2399 1862 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2039 6420 6818 7092 7239 7650 2039
Size: 4381 398 274 147 411 2376
Fragments arranged by size:
4381 2376 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3038 3068 1178
Size: 30 165 30 1635 30 6097
Fragments arranged by size:
6097 1635 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2058 4064 4153 4425 1186
Size: 872 2006 89 272 4748
Fragments arranged by size:
4748 2006 872 272 89
BseYI C'CCAG_C
Cuts at: 2062 3988 5208 7190 7686 2062
Size: 1926 1220 1982 496 2363
Fragments arranged by size:
2363 1982 1926 1220 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3071 3408 3798 5025 5124 1163
Size: 573 1335 337 390 1227 99 4026
Fragments arranged by size:
4026 1335 1227 573 390 337 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5437 6198 7226 1540
Size: 275 186 3436 761 1028 2301
Fragments arranged by size:
3436 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2374 5157 6048 6133 7204 2374
Size: 2783 891 85 1071 3157
Fragments arranged by size:
3157 2783 1071 891 85
BsmI GAATG_Cn'
Cuts at: 2515 2944 5498 5591 2515
Size: 429 2554 93 4911
Fragments arranged by size:
4911 2554 429 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2374 5157 5306 6048 6133 7204 1391
Size: 399 584 2783 149 742 85 1071 2174
Fragments arranged by size:
2783 2174 1071 742 584 399 149 85
BspEI T'CCGG_A
Cuts at: 2039 7650 2039
Size: 5611 2376
BspHI T'CATG_A
Cuts at: 2454 5876 7606 2454
Size: 3422 1730 2835
Fragments arranged by size:
3422 2835 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2716 3430 5342 844
Size: 1872 714 1912 3489
Fragments arranged by size:
3489 1912 1872 714
BsrBI CCG'CTC
Cuts at: 1394 1823 5874 1394
Size: 429 4051 3507
Fragments arranged by size:
4051 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4147 5034 6482 6656 65
Size: 1183 2899 887 1448 174 1396
Fragments arranged by size:
2899 1448 1396 1183 887 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6631 6818 1700
Size: 110 4821 187 2869
Fragments arranged by size:
4821 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7987
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7987
BssSI C'ACGA_G
Cuts at: 6047 7059 6047
Size: 1012 6975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2519 4999 794
Size: 1725 2480 3782
Fragments arranged by size:
3782 2480 1725
BstBI TT'CG_AA
Cuts at: 3949 6791 3949
Size: 2842 5145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7987
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3463 3310
Size: 153 7834
BstYI r'GATC_y
Cuts at: 1972 2140 2335 2662 2884 4462 4832 5317 5684
Size: 168 195 327 222 1578 370 485 367
Cuts at: 5684 6069 6086 7538 7624 7636 7982 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1578 1452 485 385 370 367 346
327 222 195 168 86 17 12
BstZ17I GTA'TAC
Cuts at: 2877 2877
Size: 7987
Bsu36I CC'TnA_GG
Cuts at: 4827 4827
Size: 7987
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7435
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7248
Fragments arranged by size:
7248 683 56
BtsI GCAGTG_nn'
Cuts at: 2626 3818 4078 4159 5503 6297 6317 2626
Size: 1192 260 81 1344 794 20 4296
Fragments arranged by size:
4296 1344 1192 794 260 81 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7987
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4477 4512 529
Size: 35 3913 35 4004
Fragments arranged by size:
4004 3913 35 35
DraI TTT'AAA
Cuts at: 3838 5291 3838
Size: 1453 6534
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6994 809
Size: 507 5678 1802
Fragments arranged by size:
5678 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 3453 3920
Size: 54 1425 50 275 18 435 1188 467
Cuts at: 3920 4962 5116 5434 6323 7952 8
Size: 1042 154 318 889 1629 43
Fragments arranged by size:
1629 1425 1188 1042 889 467 435 318
275 154 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5434 1537
Size: 275 3622 4090
Fragments arranged by size:
4090 3622 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 3116 3222 4041 4063 4384 4573 5917
Size: 1356 407 106 819 22 321 189 1344
Cuts at: 5917 1353
Size: 3423
Fragments arranged by size:
3423 1356 1344 819 407 321 189 106
22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6574 6948 7094 387
Size: 1064 138 4985 374 146 1280
Fragments arranged by size:
4985 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2372 2372
Size: 7987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2396 2912 3015 3140 3188 4150
Size: 393 711 53 516 103 125 48 962
Cuts at: 4150 4281 4476 4554 5210 5285 6050 6647 7434
Size: 131 195 78 656 75 765 597 787
Cuts at: 7434 7702 1239
Size: 268 1524
Fragments arranged by size:
1524 962 787 765 711 656 597 516
393 268 195 131 125 103 78 75
53 48
EcoNI CCTnn'n_nnAGG
Cuts at: 5279 5279
Size: 7987
EcoO109I rG'GnC_Cy
Cuts at: 2100 2380 2100
Size: 280 7707
EcoRI G'AATT_C
Cuts at: 4012 5296 5674 4012
Size: 1284 378 6325
Fragments arranged by size:
6325 1284 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4695 4695
Size: 7987
FspI TGC'GCA
Cuts at: 6493 6493
Size: 7987
HaeII r_GCGC'y
Cuts at: 1678 2409 3935 7134 1678
Size: 731 1526 3199 2531
Fragments arranged by size:
3199 2531 1526 731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3601 3633 4073 4105 4337
Size: 32 135 32 2029 32 440 32 232
Cuts at: 4337 4369 4522 4554 6628 6660 6702 6734 1373
Size: 32 153 32 2074 32 42 32 2626
Fragments arranged by size:
2626 2074 2029 440 232 153 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 4560 5331 5577 669
Size: 988 281 1203 1419 771 246 3079
Fragments arranged by size:
3079 1419 1203 988 771 281 246
HindIII A'AGCT_T
Cuts at: 748 2475 3170 3574 748
Size: 1727 695 404 5161
Fragments arranged by size:
5161 1727 695 404
HpaI GTT'AAC
Cuts at: 5577 5577
Size: 7987
KasI G'GCGC_C
Cuts at: 3931 3931
Size: 7987
KpnI G_GTAC'C
Cuts at: 3904 5312 3904
Size: 1408 6579
MfeI C'AATT_G
Cuts at: 5586 5586
Size: 7987
MluI A'CGCG_T
Cuts at: 7863 7863
Size: 7987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3173 3381 4921 7101 7285 1305
Size: 1868 208 1540 2180 184 2007
Fragments arranged by size:
2180 2007 1868 1540 208 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2267 3455 3922 4964 5118
Size: 54 1425 343 435 1188 467 1042 154
Cuts at: 5118 10
Size: 2879
Fragments arranged by size:
2879 1425 1188 1042 467 435 343 154
54
MslI CAynn'nnrTG
Cuts at: 518 1166 4790 5945 6304 6463 518
Size: 648 3624 1155 359 159 2042
Fragments arranged by size:
3624 2042 1155 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2968 5403 6080 7228 7473 1606
Size: 1362 2435 677 1148 245 2120
Fragments arranged by size:
2435 2120 1362 1148 677 245
NarI GG'CG_CC
Cuts at: 3932 3932
Size: 7987
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7435
NdeI CA'TA_TG
Cuts at: 387 2497 3450 387
Size: 2110 953 4924
Fragments arranged by size:
4924 2110 953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7987
NotI GC'GGCC_GC
Cuts at: 5434 5434
Size: 7987
NsiI A_TGCA'T
Cuts at: 2233 4797 2233
Size: 2564 5423
NspI r_CATG'y
Cuts at: 1132 1229 2746 4799 5345 6890 1132
Size: 97 1517 2053 546 1545 2229
Fragments arranged by size:
2229 2053 1545 1517 546 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7987
PciI A'CATG_T
Cuts at: 6886 6886
Size: 7987
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4940 1108
Size: 3832 4155
PfoI T'CCnGG_A
Cuts at: 3462 3462
Size: 7987
PmeI GTTT'AAAC
Cuts at: 5291 5291
Size: 7987
PmlI CAC'GTG
Cuts at: 3327 4618 3327
Size: 1291 6696
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6056 6088 7569 7601 6056
Size: 32 1481 32 6442
Fragments arranged by size:
6442 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2380
Size: 7987
PshAI GACnn'nnGTC
Cuts at: 1613 4195 1613
Size: 2582 5405
PsiI TTA'TAA
Cuts at: 3398 5557 3398
Size: 2159 5828
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4423 4455 4938 4970 4423
Size: 32 483 32 7440
Fragments arranged by size:
7440 483 32 32
PstI C_TGCA'G
Cuts at: 830 3609 5339 830
Size: 2779 1730 3478
Fragments arranged by size:
3478 2779 1730
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7987
PvuII CAG'CTG
Cuts at: 2968 2968
Size: 7987
SacI G_AGCT'C
Cuts at: 2374 2374
Size: 7987
SalI G'TCGA_C
Cuts at: 1936 5329 1936
Size: 3393 4594
SbfI CC_TGCA'GG
Cuts at: 5339 5339
Size: 7987
ScaI AGT'ACT
Cuts at: 4588 6235 7947 4588
Size: 1647 1712 4628
Fragments arranged by size:
4628 1712 1647
SexAI A'CCwGG_T
Cuts at: 2307 2307
Size: 7987
SfcI C'TryA_G
Cuts at: 826 1045 3285 3605 4779 5202 5335 6470 7151
Size: 219 2240 320 1174 423 133 1135 681
Cuts at: 7151 7342 826
Size: 191 1471
Fragments arranged by size:
2240 1471 1174 1135 681 423 320 219
191 133
SfoI GGC'GCC
Cuts at: 3933 3933
Size: 7987
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7987
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7987
SmaI CCC'GGG
Cuts at: 1872 5314 1872
Size: 3442 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3824 4820 6091 6992 7254 820
Size: 197 926 1881 996 1271 901 262 1553
Fragments arranged by size:
1881 1553 1271 996 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5778 493
Size: 5285 2702
SpeI A'CTAG_T
Cuts at: 152 2701 3019 3581 152
Size: 2549 318 562 4558
Fragments arranged by size:
4558 2549 562 318
SphI G_CATG'C
Cuts at: 1132 5345 1132
Size: 4213 3774
SspI AAT'ATT
Cuts at: 5 52 2278 3795 4927 5911 5
Size: 47 2226 1517 1132 984 2081
Fragments arranged by size:
2226 2081 1517 1132 984 47
StuI AGG'CCT
Cuts at: 1517 4203 1517
Size: 2686 5301
StyI C'CwwG_G
Cuts at: 513 1065 2780 4902 5426 513
Size: 552 1715 2122 524 3074
Fragments arranged by size:
3074 2122 1715 552 524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6014 6031 6184 6369 6814 325
Size: 1255 4434 17 153 185 445 1498
Fragments arranged by size:
4434 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3672 4586 6233
Size: 274 80 33 51 1443 1695 914 1647
Cuts at: 6233 7945 96
Size: 1712 138
Fragments arranged by size:
1712 1695 1647 1443 914 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2776 2808 7947 7979 2776
Size: 32 5139 32 2784
Fragments arranged by size:
5139 2784 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7987
XbaI T'CTAG_A
Cuts at: 4459 5323 4459
Size: 864 7123
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7987
XmaI C'CCGG_G
Cuts at: 1870 5312 1870
Size: 3442 4545
XmnI GAAnn'nnTTC
Cuts at: 2241 2586 3125 3531 6116 2241
Size: 345 539 406 2585 4112
Fragments arranged by size:
4112 2585 539 406 345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5795 276
Size: 53 83 186 936 4261 2468
Fragments arranged by size:
4261 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BsmBI DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PspOMI PspXI RsrII SacII SanDI SapI
SfiI SrfI SwaI XcmI