Restriction map for
pFN21AE4587
(Circular) (Six-base) MAPSORT of: pFN21AE4587.seq Check: 3675 from: 1 to: 7384
pFN21AE4587 7384 bp
With 182 enzymes: SgfI *
March 28, 2012 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2332 3400 2332
Size: 1068 6316
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3582 5194 278
Size: 53 83 186 936 2046 1612 2468
Fragments arranged by size:
2468 2046 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4727 1937
Size: 2790 4594
Acc65I G'GTAC_C
Cuts at: 4705 4705
Size: 7384
AclI AA'CG_TT
Cuts at: 1563 5511 5884 1563
Size: 3948 373 3063
Fragments arranged by size:
3948 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2701 5447 6831 7099 1632
Size: 402 667 2746 1384 268 1917
Fragments arranged by size:
2746 1917 1384 667 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7187
AflIII A'CryG_T
Cuts at: 1203 2076 6283 7260 1203
Size: 873 4207 977 1327
Fragments arranged by size:
4207 1327 977 873
AgeI A'CCGG_T
Cuts at: 6215 6215
Size: 7384
AhdI GACnn_n'nnGTC
Cuts at: 3925 6113 3925
Size: 2188 5196
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7384
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3188 3239 3875 1184
Size: 2004 51 636 4693
Fragments arranged by size:
4693 2004 636 51
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2946 2978 2946
Size: 32 7352
AlwNI CAG_nnn'CTG
Cuts at: 2524 2998 3022 4042 4363 4477 4576 5127 6699
Size: 474 24 1020 321 114 99 551 1572
Cuts at: 6699 2524
Size: 3209
Fragments arranged by size:
3209 1572 1020 551 474 321 114 99
24
ApaI G_GGCC'C
Cuts at: 2539 2837 3013 3845 4330 4563 4602 2539
Size: 298 176 832 485 233 39 5321
Fragments arranged by size:
5321 832 485 298 233 176 39
ApaLI G'TGCA_C
Cuts at: 5441 6597 5441
Size: 1156 6228
ApoI r'AATT_y
Cuts at: 1493 4693 4919 5071 7180 1493
Size: 3200 226 152 2109 1697
Fragments arranged by size:
3200 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5938 160
Size: 872 4906 1606
Fragments arranged by size:
4906 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7384
AvaI C'yCGr_G
Cuts at: 1870 1943 2202 2267 3266 3735 3870 3954 4323
Size: 73 259 65 999 469 135 84 369
Cuts at: 4323 4562 4709 1870
Size: 239 147 4545
Fragments arranged by size:
4545 999 469 369 259 239 147 135
84 73 65
BamHI G'GATC_C
Cuts at: 4714 5081 4714
Size: 367 7017
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3352 4669 4705 6160 618
Size: 325 217 627 1565 1317 36 1455 1842
Fragments arranged by size:
1842 1565 1455 1317 627 325 217 36
BanII G_rGCy'C
Cuts at: 1391 2539 2698 2837 3013 3439 3845 4281 4330
Size: 1148 159 139 176 426 406 436 49
Cuts at: 4330 4563 4602 4703 1391
Size: 233 39 101 4072
Fragments arranged by size:
4072 1148 436 426 406 233 176 159
139 101 49 39
BbeI G_GCGC'C
Cuts at: 3356 3356
Size: 7384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3439 3877 928
Size: 971 1540 438 4435
Fragments arranged by size:
4435 1540 971 438
BbvCI CC'TCA_GC
Cuts at: 3005 4368 3005
Size: 1363 6021
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 4172 4206 5575 5609 1903
Size: 34 1772 34 429 34 1369 34 3678
Fragments arranged by size:
3678 1772 1369 429 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5276 6486 5276
Size: 1210 6174
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7384
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3849 5995 136
Size: 107 122 71 631 605 2177 2146 1525
Fragments arranged by size:
2177 2146 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7379 7379
Size: 7384
BlpI GC'TnA_GC
Cuts at: 4275 4356 4801 4275
Size: 81 445 6858
Fragments arranged by size:
6858 445 81
Bme1580I G_kGCm'C
Cuts at: 1790 2539 2820 2837 3013 3393 3845 4330 4563
Size: 749 281 17 176 380 452 485 233
Cuts at: 4563 4602 5445 6601 1790
Size: 39 843 1156 2573
Fragments arranged by size:
2573 1156 843 749 485 452 380 281
233 176 39 17
BmgBI CAC'GTC
Cuts at: 1318 2171 3922 1318
Size: 853 1751 4780
Fragments arranged by size:
4780 1751 853
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2748 4621 5115
Size: 416 53 83 399 24 1331 1873 494
Cuts at: 5115 6073 442
Size: 958 1753
Fragments arranged by size:
1873 1753 1331 958 494 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 4371 3067
Size: 1304 6080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2918 3044 3113 3326 4064 4151 4265 4355
Size: 1679 126 69 213 738 87 114 90
Cuts at: 4355 4361 4454 4674 6044 1239
Size: 6 93 220 1370 2579
Fragments arranged by size:
2579 1679 1370 738 220 213 126 114
93 90 87 69 6
Bpu10I CC'TnA_GC
Cuts at: 2531 3005 3456 4368 4635 2531
Size: 474 451 912 267 5280
Fragments arranged by size:
5280 912 474 451 267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4088 4629 5509 6374 6672 4088
Size: 541 880 865 298 4800
Fragments arranged by size:
4800 880 865 541 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3055 3818 6047 882
Size: 617 1556 763 2229 2219
Fragments arranged by size:
2229 2219 1556 763 617
BsaAI yAC'GTr
Cuts at: 493 1204 2454 2782 4666 5175 493
Size: 711 1250 328 1884 509 2702
Fragments arranged by size:
2702 1884 1250 711 509 328
BsaBI GATnn'nnATC
Cuts at: 7378 7378
Size: 7384
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2655 3353 3579 3960
Size: 53 83 186 936 1122 698 226 381
Cuts at: 3960 5191 5573 6208 275
Size: 1231 382 635 1451
Fragments arranged by size:
1451 1231 1122 936 698 635 382 381
226 186 83 53
BsaWI w'CCGG_w
Cuts at: 2872 3188 3657 5817 6215 6489 6636 7047 2872
Size: 316 469 2160 398 274 147 411 3209
Fragments arranged by size:
3209 2160 469 411 398 316 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2397 2427 4169 4199 1178
Size: 30 165 30 994 30 1742 30 4363
Fragments arranged by size:
4363 1742 994 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 2759 2813 3041 3056 3143 3290 3494
Size: 1462 111 54 228 15 87 147 204
Cuts at: 3494 1186
Size: 5076
Fragments arranged by size:
5076 1462 228 204 147 111 87 54
15
BseYI C'CCAG_C
Cuts at: 2550 2620 2806 2890 3062 3469 3509 3556 4329
Size: 70 186 84 172 407 40 47 773
Cuts at: 4329 4415 4442 4549 4601 6587 7083 2550
Size: 86 27 107 52 1986 496 2851
Fragments arranged by size:
2851 1986 773 496 407 186 172 107
86 84 70 52 47 40 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2164 2669 2783 3194 3428 3944 1163
Size: 573 428 505 114 411 234 516 4603
Fragments arranged by size:
4603 573 516 505 428 411 234 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4063 4834 5595 6623 1540
Size: 275 186 2062 771 761 1028 2301
Fragments arranged by size:
2301 2062 1028 771 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4160 4281 5445 5530 6601 4160
Size: 121 1164 85 1071 4943
Fragments arranged by size:
4943 1164 1071 121 85
BsmI GAATG_Cn'
Cuts at: 4895 4988 4895
Size: 93 7291
BsmBI CGTCTCn'nnnn_
Cuts at: 3668 3668
Size: 7384
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2539 2698 2820 2837 3013 3393 3439
Size: 399 749 159 122 17 176 380 46
Cuts at: 3439 3845 4160 4281 4330 4563 4602 4703 5445
Size: 406 315 121 49 233 39 101 742
Cuts at: 5445 5530 6601 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 749 742 406 399 380 315
233 176 159 122 121 101 85 49
46 39 17
BspEI T'CCGG_A
Cuts at: 7047 7047
Size: 7384
BspHI T'CATG_A
Cuts at: 5273 7003 5273
Size: 1730 5654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2332 3400 4447 4739 844
Size: 1488 1068 1047 292 3489
Fragments arranged by size:
3489 1488 1068 1047 292
BsrBI CCG'CTC
Cuts at: 1394 1823 4072 5271 1394
Size: 429 2249 1199 3507
Fragments arranged by size:
3507 2249 1199 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5879 6053 65
Size: 1183 4631 174 1396
Fragments arranged by size:
4631 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4062 6028 6215 1700
Size: 110 2252 1966 187 2869
Fragments arranged by size:
2869 2252 1966 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7384
BssHII G'CGCG_C
Cuts at: 1925 3199 1925
Size: 1274 6110
BssSI C'ACGA_G
Cuts at: 2795 5444 6456 2795
Size: 2649 1012 3723
Fragments arranged by size:
3723 2649 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2547 2998 794
Size: 1753 451 5180
Fragments arranged by size:
5180 1753 451
BstBI TT'CG_AA
Cuts at: 6188 6188
Size: 7384
BstEII G'GTnAC_C
Cuts at: 1184 2155 2596 1184
Size: 971 441 5972
Fragments arranged by size:
5972 971 441
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 7384
BstYI r'GATC_y
Cuts at: 1972 2271 4080 4714 5081 5466 5483 6935 7021
Size: 299 1809 634 367 385 17 1452 86
Cuts at: 7021 7033 7379 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1809 1452 634 385 367 346 299
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3477 3477
Size: 7384
BtgI C'CryG_G
Cuts at: 513 1065 2004 3082 3750 4057 4149 513
Size: 552 939 1078 668 307 92 3748
Fragments arranged by size:
3748 1078 939 668 552 307 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3712 487
Size: 683 56 2486 4159
Fragments arranged by size:
4159 2486 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 3804 3919 4900 5694 5714 2358
Size: 1446 115 981 794 20 4028
Fragments arranged by size:
4028 1446 981 794 115 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7384
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7349
DraI TTT'AAA
Cuts at: 4688 4688
Size: 7384
DraIII CAC_nnn'GTG
Cuts at: 4669 4669
Size: 7384
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3881 6391 809
Size: 507 2565 2510 1802
Fragments arranged by size:
2565 2510 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 3678 3984
Size: 54 1425 50 275 18 357 1491 306
Cuts at: 3984 4020 4060 4146 4257 4590 4831 5720 7349
Size: 36 40 86 111 333 241 889 1629
Cuts at: 7349 8
Size: 43
Fragments arranged by size:
1629 1491 1425 889 357 333 306 275
241 111 86 54 50 43 40 36
18
EagI C'GGCC_G
Cuts at: 1537 1812 4060 4831 1537
Size: 275 2248 771 4090
Fragments arranged by size:
4090 2248 771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 4532 5314 1353
Size: 657 2522 782 3423
Fragments arranged by size:
3423 2522 782 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2716 3614 4190 5971 6345 6491
Size: 1064 138 1127 898 576 1781 374 146
Cuts at: 6491 387
Size: 1280
Fragments arranged by size:
1781 1280 1127 1064 898 576 374 146
138
EcoICRI GAG'CTC
Cuts at: 4279 4279
Size: 7384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2701 2918 3044 3113 3326 4064
Size: 393 402 667 217 126 69 213 738
Cuts at: 4064 4151 4265 4355 4361 4454 4674 5447 6044
Size: 87 114 90 6 93 220 773 597
Cuts at: 6044 6831 7099 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 773 738 667 597 402 393
268 220 217 213 126 114 93 90
87 69 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3026 3026
Size: 7384
EcoO109I rG'GnC_Cy
Cuts at: 2287 2444 2535 2834 3009 3010 3298 3473 3560
Size: 157 91 299 175 1 288 175 87
Cuts at: 3560 3664 3841 3842 3867 3951 4412 4429 4559
Size: 104 177 1 25 84 461 17 130
Cuts at: 4559 4639 2287
Size: 80 5032
Fragments arranged by size:
5032 461 299 288 177 175 175 157
130 104 91 87 84 80 25 17
1 1
EcoRI G'AATT_C
Cuts at: 4693 5071 4693
Size: 378 7006
EcoRV GAT'ATC
Cuts at: 4620 4620
Size: 7384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2267 2267
Size: 7384
FspI TGC'GCA
Cuts at: 2150 5890 2150
Size: 3740 3644
FspAI rTGC'GCAy
Cuts at: 2150 2150
Size: 7384
HaeII r_GCGC'y
Cuts at: 1678 2381 3136 3356 6531 1678
Size: 703 755 220 3175 2531
Fragments arranged by size:
3175 2531 755 703 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3449 3481 3911 3943 4169
Size: 32 135 32 1877 32 430 32 226
Cuts at: 4169 4201 4604 4636 6025 6057 6099 6131 1373
Size: 32 403 32 1389 32 42 32 2626
Fragments arranged by size:
2626 1877 1389 430 403 226 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4728 4974 669
Size: 988 281 2790 246 3079
Fragments arranged by size:
3079 2790 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7384
HpaI GTT'AAC
Cuts at: 4974 4974
Size: 7384
KasI G'GCGC_C
Cuts at: 3352 3352
Size: 7384
KpnI G_GTAC'C
Cuts at: 4709 4709
Size: 7384
MfeI C'AATT_G
Cuts at: 4983 4983
Size: 7384
MluI A'CGCG_T
Cuts at: 7260 7260
Size: 7384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2848 2964 6498 6682 1305
Size: 1543 116 3534 184 2007
Fragments arranged by size:
3534 2007 1543 184 116
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 3986 4259 4592 10
Size: 54 1425 343 357 1797 273 333 2802
Fragments arranged by size:
2802 1797 1425 357 343 333 273 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 3170 3221 4148 5342 5701 5860
Size: 648 984 1020 51 927 1194 359 159
Cuts at: 5860 518
Size: 2042
Fragments arranged by size:
2042 1194 1020 984 927 648 359 159
51
MspA1I CmG'CkG
Cuts at: 1606 2624 2890 2950 3084 3509 3625 3982 4059
Size: 1018 266 60 134 425 116 357 77
Cuts at: 4059 4333 4363 4800 5477 6625 6870 1606
Size: 274 30 437 677 1148 245 2120
Fragments arranged by size:
2120 1148 1018 677 437 425 357 274
266 245 134 116 77 60 30
NaeI GCC'GGC
Cuts at: 4064 4064
Size: 7384
NarI GG'CG_CC
Cuts at: 3353 3353
Size: 7384
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5893
Fragments arranged by size:
5893 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7384
NgoMIV G'CCGG_C
Cuts at: 4062 4062
Size: 7384
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7384
NotI GC'GGCC_GC
Cuts at: 4831 4831
Size: 7384
NspI r_CATG'y
Cuts at: 1132 1229 3226 4742 6287 1132
Size: 97 1997 1516 1545 2229
Fragments arranged by size:
2229 1997 1545 1516 97
PasI CC'CwG_GG
Cuts at: 1348 2212 2306 2830 4477 4576 4607 1348
Size: 864 94 524 1647 99 31 4125
Fragments arranged by size:
4125 1647 864 524 99 94 31
PciI A'CATG_T
Cuts at: 6283 6283
Size: 7384
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 2218 3349 4477 1108
Size: 951 159 1131 1128 4015
Fragments arranged by size:
4015 1131 1128 951 159
PfoI T'CCnGG_A
Cuts at: 2709 2964 4083 4651 2709
Size: 255 1119 568 5442
Fragments arranged by size:
5442 1119 568 255
PmeI GTTT'AAAC
Cuts at: 4688 4688
Size: 7384
PmlI CAC'GTG
Cuts at: 2782 4666 2782
Size: 1884 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 5453 5485 6966 6998 3470
Size: 32 1951 32 1481 32 3856
Fragments arranged by size:
3856 1951 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 2444 3298 3473 3664 3951 4429 2287
Size: 157 854 175 191 287 478 5242
Fragments arranged by size:
5242 854 478 287 191 175 157
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7384
PsiI TTA'TAA
Cuts at: 4954 4954
Size: 7384
PspOMI G'GGCC_C
Cuts at: 2535 2833 3009 3841 4326 4559 4598 2535
Size: 298 176 832 485 233 39 5321
Fragments arranged by size:
5321 832 485 298 233 176 39
PstI C_TGCA'G
Cuts at: 830 2343 3018 3624 4233 4736 830
Size: 1513 675 606 609 503 3478
Fragments arranged by size:
3478 1513 675 609 606 503
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7384
PvuII CAG'CTG
Cuts at: 2890 2950 3509 3625 3982 4333 4363 2890
Size: 60 559 116 357 351 30 5911
Fragments arranged by size:
5911 559 357 351 116 60 30
RsrII CG'GwC_CG
Cuts at: 2870 2870
Size: 7384
SacI G_AGCT'C
Cuts at: 4281 4281
Size: 7384
SacII CC_GC'GG
Cuts at: 3085 4060 3085
Size: 975 6409
SalI G'TCGA_C
Cuts at: 1936 4726 1936
Size: 2790 4594
SbfI CC_TGCA'GG
Cuts at: 2343 4736 2343
Size: 2393 4991
ScaI AGT'ACT
Cuts at: 5632 7344 5632
Size: 1712 5672
SfcI C'TryA_G
Cuts at: 826 1045 2339 3014 3620 4229 4732 5867 6548
Size: 219 1294 675 606 609 503 1135 681
Cuts at: 6548 6739 826
Size: 191 1471
Fragments arranged by size:
1471 1294 1135 681 675 609 606 503
219 191
SfoI GGC'GCC
Cuts at: 3354 3354
Size: 7384
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7384
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7384
SmaI CCC'GGG
Cuts at: 1872 3268 3737 3872 3956 4325 4711 1872
Size: 1396 469 135 84 369 386 4545
Fragments arranged by size:
4545 1396 469 386 369 135 84
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 4067 4644 5488 6389 6651
Size: 197 926 324 1800 577 844 901 262
Cuts at: 6651 820
Size: 1553
Fragments arranged by size:
1800 1553 926 901 844 577 324 262
197
SnaBI TAC'GTA
Cuts at: 493 5175 493
Size: 4682 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7384
SphI G_CATG'C
Cuts at: 1132 4742 1132
Size: 3610 3774
SrfI GCCC'GGGC
Cuts at: 4325 4325
Size: 7384
SspI AAT'ATT
Cuts at: 5 52 5308 5
Size: 47 5256 2081
Fragments arranged by size:
5256 2081 47
StuI AGG'CCT
Cuts at: 1517 4253 1517
Size: 2736 4648
StyI C'CwwG_G
Cuts at: 513 1065 2004 3348 4170 4254 4823 513
Size: 552 939 1344 822 84 569 3074
Fragments arranged by size:
3074 1344 939 822 569 552 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5411 5428 5581 5766 6211 325
Size: 1255 3831 17 153 185 445 1498
Fragments arranged by size:
3831 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4675 5630 7342
Size: 274 80 33 51 1443 2698 955 1712
Cuts at: 7342 96
Size: 138
Fragments arranged by size:
2698 1712 1443 955 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 7344 7376 2397
Size: 32 4915 32 2405
Fragments arranged by size:
4915 2405 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2657 2864 3239 1316
Size: 1341 207 375 5461
Fragments arranged by size:
5461 1341 375 207
XbaI T'CTAG_A
Cuts at: 3148 4720 3148
Size: 1572 5812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2827 3118 2827
Size: 291 7093
XhoI C'TCGA_G
Cuts at: 1943 2267 1943
Size: 324 7060
XmaI C'CCGG_G
Cuts at: 1870 3266 3735 3870 3954 4323 4709 1870
Size: 1396 469 135 84 369 386 4545
Fragments arranged by size:
4545 1396 469 386 369 135 84
XmnI GAAnn'nnTTC
Cuts at: 4385 5513 4385
Size: 1128 6256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3580 5192 276
Size: 53 83 186 936 2046 1612 2468
Fragments arranged by size:
2468 2046 1612 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI
NruI NsiI PacI PspXI PsrI SanDI SapI SexAI
SfiI SwaI