Restriction map for
pFN21AE4586
(Circular) (Six-base) MAPSORT of: pFN21AE4586.seq Check: 3697 from: 1 to: 7375
pFN21AE4586 7375 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2575 2575
Size: 7375
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4398 5185 278
Size: 53 83 186 936 2862 787 2468
Fragments arranged by size:
2862 2468 936 787 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3531 3981 4718 1937
Size: 1594 450 737 4594
Fragments arranged by size:
4594 1594 737 450
Acc65I G'GTAC_C
Cuts at: 4696 4696
Size: 7375
AclI AA'CG_TT
Cuts at: 1563 5502 5875 1563
Size: 3939 373 3063
Fragments arranged by size:
3939 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2882 3467 5438 6822 7090 1632
Size: 1250 585 1971 1384 268 1917
Fragments arranged by size:
1971 1917 1384 1250 585 268
AflII C'TTAA_G
Cuts at: 820 1017 4274 820
Size: 197 3257 3921
Fragments arranged by size:
3921 3257 197
AflIII A'CryG_T
Cuts at: 1203 6274 7251 1203
Size: 5071 977 1327
Fragments arranged by size:
5071 1327 977
AgeI A'CCGG_T
Cuts at: 6206 6206
Size: 7375
AhdI GACnn_n'nnGTC
Cuts at: 6104 6104
Size: 7375
AleI CACnn'nnGTG
Cuts at: 1166 2381 1166
Size: 1215 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4029 4631 1184
Size: 2845 602 3928
Fragments arranged by size:
3928 2845 602
AlwNI CAG_nnn'CTG
Cuts at: 5118 6690 5118
Size: 1572 5803
ApaI G_GGCC'C
Cuts at: 2033 3379 2033
Size: 1346 6029
ApaLI G'TGCA_C
Cuts at: 5432 6588 5432
Size: 1156 6219
ApoI r'AATT_y
Cuts at: 1493 3571 4684 4910 5062 7171 1493
Size: 2078 1113 226 152 2109 1697
Fragments arranged by size:
2109 2078 1697 1113 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5929 160
Size: 872 4897 1606
Fragments arranged by size:
4897 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 4700 1870
Size: 73 184 2573 4545
Fragments arranged by size:
4545 2573 184 73
BamHI G'GATC_C
Cuts at: 4705 5072 4705
Size: 367 7008
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 3581 4622 4696 6151
Size: 325 217 627 444 1350 1041 74 1455
Cuts at: 6151 618
Size: 1842
Fragments arranged by size:
1842 1455 1350 1041 627 444 325 217
74
BanII G_rGCy'C
Cuts at: 1391 2033 2840 3162 3216 3363 3379 3633 3786
Size: 642 807 322 54 147 16 254 153
Cuts at: 3786 3896 4266 4530 4694 1391
Size: 110 370 264 164 4072
Fragments arranged by size:
4072 807 642 370 322 264 254 164
153 147 110 54 16
BbeI G_GCGC'C
Cuts at: 4626 4626
Size: 7375
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3232 4513 928
Size: 971 1333 1281 3790
Fragments arranged by size:
3790 1333 1281 971
BbvCI CC'TCA_GC
Cuts at: 3566 3566
Size: 7375
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2399 2433 4453 4487 5566 5600 1903
Size: 34 462 34 2020 34 1079 34 3678
Fragments arranged by size:
3678 2020 1079 462 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5267 6477 5267
Size: 1210 6165
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7375
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2930 3734 4209
Size: 107 122 71 631 605 1258 804 475
Cuts at: 4209 4503 5986 136
Size: 294 1483 1525
Fragments arranged by size:
1525 1483 1258 804 631 605 475 294
122 107 71
BglII A'GATC_T
Cuts at: 7370 7370
Size: 7375
BlpI GC'TnA_GC
Cuts at: 4448 4792 4448
Size: 344 7031
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2664 3379 3584 3603 3825 5436 6592
Size: 243 631 715 205 19 222 1611 1156
Cuts at: 6592 1790
Size: 2573
Fragments arranged by size:
2573 1611 1156 715 631 243 222 205
19
BmgBI CAC'GTC
Cuts at: 1318 2472 3774 1318
Size: 1154 1302 4919
Fragments arranged by size:
4919 1302 1154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5106 6064 442
Size: 416 53 83 399 24 3689 958 1753
Fragments arranged by size:
3689 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2540 2627 2714 2911 3230 3671 3800
Size: 1061 240 87 87 197 319 441 129
Cuts at: 3800 4103 4511 6035 1239
Size: 303 408 1524 2579
Fragments arranged by size:
2579 1524 1061 441 408 319 303 240
197 129 87 87
Bpu10I CC'TnA_GC
Cuts at: 3338 3344 3566 3338
Size: 6 222 7147
Fragments arranged by size:
7147 222 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3074 4514 5500 6365 6663 3074
Size: 1440 986 865 298 3786
Fragments arranged by size:
3786 1440 986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2504 4037 6038 882
Size: 617 1005 1533 2001 2219
Fragments arranged by size:
2219 2001 1533 1005 617
BsaAI yAC'GTr
Cuts at: 493 1204 4101 4585 5166 493
Size: 711 2897 484 581 2702
Fragments arranged by size:
2897 2702 711 581 484
BsaBI GATnn'nnATC
Cuts at: 7369 7369
Size: 7375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2775 4395 4623 5182
Size: 53 83 186 936 1242 1620 228 559
Cuts at: 5182 5564 6199 275
Size: 382 635 1451
Fragments arranged by size:
1620 1451 1242 936 635 559 382 228
186 83 53
BsaWI w'CCGG_w
Cuts at: 5808 6206 6480 6627 7038 5808
Size: 398 274 147 411 6145
Fragments arranged by size:
6145 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2909 2939 1178
Size: 30 165 30 1506 30 5614
Fragments arranged by size:
5614 1506 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2494 4271 4274 4400 1186
Size: 942 366 1777 3 126 4161
Fragments arranged by size:
4161 1777 942 366 126 3
BseYI C'CCAG_C
Cuts at: 2026 2199 2783 4560 6578 7074 2026
Size: 173 584 1777 2018 496 2327
Fragments arranged by size:
2327 2018 1777 584 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2359 2867 3067 1163
Size: 573 623 508 200 5471
Fragments arranged by size:
5471 623 573 508 200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4111 4254 4318 4674 4825 5586
Size: 275 186 2110 143 64 356 151 761
Cuts at: 5586 6614 1540
Size: 1028 2301
Fragments arranged by size:
2301 2110 1028 761 356 275 186 151
143 64
BsiHKAI G_wGCw'C
Cuts at: 2134 2634 3350 3633 3786 3896 4266 4530 4599
Size: 500 716 283 153 110 370 264 69
Cuts at: 4599 5436 5521 6592 2134
Size: 837 85 1071 2917
Fragments arranged by size:
2917 1071 837 716 500 370 283 264
153 110 85 69
BsmI GAATG_Cn'
Cuts at: 4886 4979 4886
Size: 93 7282
BsmBI CGTCTCn'nnnn_
Cuts at: 2163 3780 2163
Size: 1617 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2134 2634 2664 2840 3162 3216
Size: 399 243 101 500 30 176 322 54
Cuts at: 3216 3350 3363 3379 3584 3603 3633 3786 3825
Size: 134 13 16 205 19 30 153 39
Cuts at: 3825 3896 4266 4530 4599 4694 5436 5521 6592
Size: 71 370 264 69 95 742 85 1071
Cuts at: 6592 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 500 399 370 322 264
243 205 176 153 134 101 95 85
71 69 54 39 30 30 19 16
13
BspEI T'CCGG_A
Cuts at: 7038 7038
Size: 7375
BspHI T'CATG_A
Cuts at: 5264 6994 5264
Size: 1730 5645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2343 2575 2856 3150 3543 4730 844
Size: 1499 232 281 294 393 1187 3489
Fragments arranged by size:
3489 1499 1187 393 294 281 232
BsrBI CCG'CTC
Cuts at: 1394 1823 3963 5262 1394
Size: 429 2140 1299 3507
Fragments arranged by size:
3507 2140 1299 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5870 6044 65
Size: 1183 4622 174 1396
Fragments arranged by size:
4622 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2345 2442 3311 3398 3659 3728 4106
Size: 110 535 97 869 87 261 69 378
Cuts at: 4106 6019 6206 1700
Size: 1913 187 2869
Fragments arranged by size:
2869 1913 869 535 378 261 187 110
97 87 69
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7375
BssHII G'CGCG_C
Cuts at: 1925 4055 1925
Size: 2130 5245
BssSI C'ACGA_G
Cuts at: 5435 6447 5435
Size: 1012 6363
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 3279 3288 3588 794
Size: 1541 944 9 300 4581
Fragments arranged by size:
4581 1541 944 300 9
BstBI TT'CG_AA
Cuts at: 6179 6179
Size: 7375
BstEII G'GTnAC_C
Cuts at: 1184 3391 1184
Size: 2207 5168
BstXI CCAn_nnnn'nTGG
Cuts at: 3664 3724 4035 3664
Size: 60 311 7004
Fragments arranged by size:
7004 311 60
BstYI r'GATC_y
Cuts at: 1972 4065 4705 5072 5457 5474 6926 7012 7024
Size: 2093 640 367 385 17 1452 86 12
Cuts at: 7024 7370 1972
Size: 346 1977
Fragments arranged by size:
2093 1977 1452 640 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2841 2841
Size: 7375
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 3072 3636 3804 3897 4248
Size: 552 939 588 480 564 168 93 351
Cuts at: 4248 4338 513
Size: 90 3550
Fragments arranged by size:
3550 939 588 564 552 480 351 168
93 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2713 3250 487
Size: 683 56 1487 537 4612
Fragments arranged by size:
4612 1487 683 537 56
BtsI GCAGTG_nn'
Cuts at: 2016 2852 4891 5685 5705 2016
Size: 836 2039 794 20 3686
Fragments arranged by size:
3686 2039 836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7375
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7340
DraI TTT'AAA
Cuts at: 4679 4679
Size: 7375
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2393 6382 809
Size: 507 1077 3989 1802
Fragments arranged by size:
3989 1802 1077 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 2589 2617
Size: 54 1425 50 275 18 513 246 28
Cuts at: 2617 3381 3457 3484 3726 3927 4108 4251 4284
Size: 764 76 27 242 201 181 143 33
Cuts at: 4284 4671 4822 5711 7340 8
Size: 387 151 889 1629 43
Fragments arranged by size:
1629 1425 889 764 513 387 275 246
242 201 181 151 143 76 54 50
43 33 28 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 4251 4671 4822 1537
Size: 275 2296 143 420 151 4090
Fragments arranged by size:
4090 2296 420 275 151 143
EarI CTCTTCn'nnn_
Cuts at: 1353 2455 2828 3551 3704 5305 1353
Size: 1102 373 723 153 1601 3423
Fragments arranged by size:
3423 1601 1102 723 373 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2468 2612 5962 6336 6482 387
Size: 1064 138 879 144 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 879 374 146 144 138
EcoICRI GAG'CTC
Cuts at: 3631 3784 3894 4264 4528 3631
Size: 153 110 370 264 6478
Fragments arranged by size:
6478 370 264 153 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2540 2627 2714 2882 2911 3230
Size: 393 668 240 87 87 168 29 319
Cuts at: 3230 3467 3671 3800 4103 4511 5438 6035 6822
Size: 237 204 129 303 408 927 597 787
Cuts at: 6822 7090 1239
Size: 268 1524
Fragments arranged by size:
1524 927 787 668 597 408 393 319
303 268 240 237 204 168 129 87
87 29
EcoNI CCTnn'n_nnAGG
Cuts at: 4457 4457
Size: 7375
EcoO109I rG'GnC_Cy
Cuts at: 2030 2124 2299 2654 2845 3375 3376 3451 2030
Size: 94 175 355 191 530 1 75 5954
Fragments arranged by size:
5954 530 355 191 175 94 75 1
EcoRI G'AATT_C
Cuts at: 4684 5062 4684
Size: 378 6997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3871 4654 3871
Size: 783 6592
FseI GG_CCGG'CC
Cuts at: 3732 3732
Size: 7375
FspI TGC'GCA
Cuts at: 2534 5881 2534
Size: 3347 4028
HaeII r_GCGC'y
Cuts at: 1678 2701 4152 4365 4626 6522 1678
Size: 1023 1451 213 261 1896 2531
Fragments arranged by size:
2531 1896 1451 1023 261 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2941 6016 6048 6090
Size: 32 135 32 1337 32 3075 32 42
Cuts at: 6090 6122 1373
Size: 32 2626
Fragments arranged by size:
3075 2626 1337 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 3190 4719 4965 669
Size: 988 281 688 564 1529 246 3079
Fragments arranged by size:
3079 1529 988 688 564 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7375
HpaI GTT'AAC
Cuts at: 4965 4965
Size: 7375
KasI G'GCGC_C
Cuts at: 4622 4622
Size: 7375
KpnI G_GTAC'C
Cuts at: 4700 4700
Size: 7375
MfeI C'AATT_G
Cuts at: 4974 4974
Size: 7375
MluI A'CGCG_T
Cuts at: 7251 7251
Size: 7375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2082 6489 6673 1305
Size: 732 45 4407 184 2007
Fragments arranged by size:
4407 2007 732 184 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3383 3459 4286 10
Size: 54 1425 343 1551 76 827 3099
Fragments arranged by size:
3099 1551 1425 827 343 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3071 3473 3722 3914 4166 5333
Size: 648 1215 690 402 249 192 252 1167
Cuts at: 5333 5692 5851 518
Size: 359 159 2042
Fragments arranged by size:
2042 1215 1167 690 648 402 359 252
249 192 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2177 2186 2373 2381 3043 3565 4359
Size: 420 151 9 187 8 662 522 794
Cuts at: 4359 4542 4791 5468 6616 6861 1606
Size: 183 249 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 662 522 420 249
245 187 183 151 9 8
NaeI GCC'GGC
Cuts at: 2347 3400 3730 2347
Size: 1053 330 5992
Fragments arranged by size:
5992 1053 330
NarI GG'CG_CC
Cuts at: 4623 4623
Size: 7375
NcoI C'CATG_G
Cuts at: 513 1065 2004 3072 3897 513
Size: 552 939 1068 825 3991
Fragments arranged by size:
3991 1068 939 825 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7375
NgoMIV G'CCGG_C
Cuts at: 2345 3398 3728 2345
Size: 1053 330 5992
Fragments arranged by size:
5992 1053 330
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7375
NotI GC'GGCC_GC
Cuts at: 4822 4822
Size: 7375
NruI TCG'CGA
Cuts at: 2421 2421
Size: 7375
NspI r_CATG'y
Cuts at: 1132 1229 2692 2941 4171 4348 4733 6278 1132
Size: 97 1463 249 1230 177 385 1545 2229
Fragments arranged by size:
2229 1545 1463 1230 385 249 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7375
PciI A'CATG_T
Cuts at: 6274 6274
Size: 7375
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7375
PfoI T'CCnGG_A
Cuts at: 2277 3099 4488 2277
Size: 822 1389 5164
Fragments arranged by size:
5164 1389 822
PmeI GTTT'AAAC
Cuts at: 4679 4679
Size: 7375
PmlI CAC'GTG
Cuts at: 4101 4585 4101
Size: 484 6891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5444 5476 6957 6989 5444
Size: 32 1481 32 5830
Fragments arranged by size:
5830 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2845 3451 2299
Size: 546 606 6223
Fragments arranged by size:
6223 606 546
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7375
PsiI TTA'TAA
Cuts at: 4945 4945
Size: 7375
PspOMI G'GGCC_C
Cuts at: 2029 3375 2029
Size: 1346 6029
PstI C_TGCA'G
Cuts at: 830 2340 2676 2802 3756 4076 4232 4727 830
Size: 1510 336 126 954 320 156 495 3478
Fragments arranged by size:
3478 1510 954 495 336 320 156 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7375
PvuII CAG'CTG
Cuts at: 2373 3565 4542 2373
Size: 1192 977 5206
Fragments arranged by size:
5206 1192 977
SacI G_AGCT'C
Cuts at: 3633 3786 3896 4266 4530 3633
Size: 153 110 370 264 6478
Fragments arranged by size:
6478 370 264 153 110
SalI G'TCGA_C
Cuts at: 1936 4717 1936
Size: 2781 4594
SanDI GG'GwC_CC
Cuts at: 2299 2299
Size: 7375
SapI GCTCTTCn'nnn_
Cuts at: 2828 2828
Size: 7375
SbfI CC_TGCA'GG
Cuts at: 2802 4727 2802
Size: 1925 5450
ScaI AGT'ACT
Cuts at: 5623 7335 5623
Size: 1712 5663
SexAI A'CCwGG_T
Cuts at: 3489 3606 3489
Size: 117 7258
SfcI C'TryA_G
Cuts at: 826 1045 2336 2672 2798 3752 3814 4072 4228
Size: 219 1291 336 126 954 62 258 156
Cuts at: 4228 4723 5858 6539 6730 826
Size: 495 1135 681 191 1471
Fragments arranged by size:
1471 1291 1135 954 681 495 336 258
219 191 156 126 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 3734 3734
Size: 7375
SfoI GGC'GCC
Cuts at: 4624 4624
Size: 7375
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
SgrAI Cr'CCGG_yG
Cuts at: 1700 2442 1700
Size: 742 6633
SmaI CCC'GGG
Cuts at: 1872 4702 1872
Size: 2830 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3089 4274 4529 5479 6380 6642
Size: 197 926 1146 1185 255 950 901 262
Cuts at: 6642 820
Size: 1553
Fragments arranged by size:
1553 1185 1146 950 926 901 262 255
197
SnaBI TAC'GTA
Cuts at: 493 5166 493
Size: 4673 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7375
SphI G_CATG'C
Cuts at: 1132 2692 2941 4348 4733 1132
Size: 1560 249 1407 385 3774
Fragments arranged by size:
3774 1560 1407 385 249
SspI AAT'ATT
Cuts at: 5 52 5299 5
Size: 47 5247 2081
Fragments arranged by size:
5247 2081 47
StuI AGG'CCT
Cuts at: 1517 3113 4217 1517
Size: 1596 1104 4675
Fragments arranged by size:
4675 1596 1104
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2304 2769 3072 3454 3846
Size: 552 939 285 15 465 303 382 392
Cuts at: 3846 3897 4401 4814 513
Size: 51 504 413 3074
Fragments arranged by size:
3074 939 552 504 465 413 392 382
303 285 51 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2528 3365 4076 4585 5402 5419 5572
Size: 1255 948 837 711 509 817 17 153
Cuts at: 5572 5757 6202 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 948 837 817 711 509 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2553 5621 7333
Size: 274 80 33 51 1443 576 3068 1712
Cuts at: 7333 96
Size: 138
Fragments arranged by size:
3068 1712 1443 576 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 7335 7367 2933
Size: 32 4370 32 2941
Fragments arranged by size:
4370 2941 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4394 1316
Size: 3078 4297
XbaI T'CTAG_A
Cuts at: 4711 4711
Size: 7375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2150 3169 3859 2098
Size: 52 1019 690 5614
Fragments arranged by size:
5614 1019 690 52
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7375
XmaI C'CCGG_G
Cuts at: 1870 4700 1870
Size: 2830 4545
XmnI GAAnn'nnTTC
Cuts at: 5504 5504
Size: 7375
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4396 5183 276
Size: 53 83 186 936 2862 787 2468
Fragments arranged by size:
2862 2468 936 787 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FspAI NsiI PacI PspXI PsrI
RsrII SacII SrfI SwaI