Restriction map for
pFN21AE4584
(Circular) (Six-base) MAPSORT of: pFN21AE4584.seq Check: 3598 from: 1 to: 7264
pFN21AE4584 7264 bp
With 182 enzymes: SgfI *
March 28, 2012 14:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2562 5074 278
Size: 53 83 186 936 1026 2512 2468
Fragments arranged by size:
2512 2468 1026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4607 1937
Size: 2670 4594
Acc65I G'GTAC_C
Cuts at: 3654 4585 3654
Size: 931 6333
AclI AA'CG_TT
Cuts at: 1563 5391 5764 1563
Size: 3828 373 3063
Fragments arranged by size:
3828 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2467 2684 3491 3758 5327 6711 6979 1632
Size: 835 217 807 267 1569 1384 268 1917
Fragments arranged by size:
1917 1569 1384 835 807 268 267 217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7067
AflIII A'CryG_T
Cuts at: 1203 2868 6163 7140 1203
Size: 1665 3295 977 1327
Fragments arranged by size:
3295 1665 1327 977
AgeI A'CCGG_T
Cuts at: 6095 6095
Size: 7264
AhdI GACnn_n'nnGTC
Cuts at: 2872 5993 2872
Size: 3121 4143
AleI CACnn'nnGTG
Cuts at: 1166 4346 1166
Size: 3180 4084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 2279 1184
Size: 1011 84 6169
Fragments arranged by size:
6169 1011 84
AlwNI CAG_nnn'CTG
Cuts at: 2719 3277 3946 5007 6579 2719
Size: 558 669 1061 1572 3404
Fragments arranged by size:
3404 1572 1061 669 558
ApaI G_GGCC'C
Cuts at: 2912 3022 3121 3492 2912
Size: 110 99 371 6684
Fragments arranged by size:
6684 371 110 99
ApaLI G'TGCA_C
Cuts at: 3535 4339 5321 6477 3535
Size: 804 982 1156 4322
Fragments arranged by size:
4322 1156 982 804
ApoI r'AATT_y
Cuts at: 1493 2644 2858 3104 3512 4515 4573 4799 4951
Size: 1151 214 246 408 1003 58 226 152
Cuts at: 4951 7060 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1151 1003 408 246 226 214
152 58
AseI AT'TA_AT
Cuts at: 160 1032 5818 160
Size: 872 4786 1606
Fragments arranged by size:
4786 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7264
AvaI C'yCGr_G
Cuts at: 1870 1943 2905 4589 1870
Size: 73 962 1684 4545
Fragments arranged by size:
4545 1684 962 73
BamHI G'GATC_C
Cuts at: 4594 4961 4594
Size: 367 6897
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2247 2374 3654 3681
Size: 325 217 627 334 126 127 1280 27
Cuts at: 3681 3812 3885 4585 6040 618
Size: 131 73 700 1455 1842
Fragments arranged by size:
1842 1455 1280 700 627 334 325 217
131 127 126 73 27
BanII G_rGCy'C
Cuts at: 1391 2090 2505 2912 3022 3121 3492 4305 4583
Size: 699 415 407 110 99 371 813 278
Cuts at: 4583 1391
Size: 4072
Fragments arranged by size:
4072 813 699 415 407 371 278 110
99
BbeI G_GCGC'C
Cuts at: 2251 3685 2251
Size: 1434 5830
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 2389 2622 2932 4536 928
Size: 971 325 165 233 310 1604 3656
Fragments arranged by size:
3656 1604 971 325 310 233 165
BbvCI CC'TCA_GC
Cuts at: 3004 3004
Size: 7264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2542 2576 3325 3359 4145 4179 5455
Size: 34 605 34 749 34 786 34 1276
Cuts at: 5455 5489 1903
Size: 34 3678
Fragments arranged by size:
3678 1276 786 749 605 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4568 5156 6366 4568
Size: 588 1210 5466
Fragments arranged by size:
5466 1210 588
BclI T'GATC_A
Cuts at: 1548 2571 1548
Size: 1023 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 5875 136
Size: 107 122 71 631 605 649 3554 1525
Fragments arranged by size:
3554 1525 649 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2667 7259 2667
Size: 4592 2672
BlpI GC'TnA_GC
Cuts at: 3855 4681 3855
Size: 826 6438
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2379 2477 2912 3022 3121 3492 3539
Size: 336 253 98 435 110 99 371 47
Cuts at: 3539 4343 5325 6481 1790
Size: 804 982 1156 2573
Fragments arranged by size:
2573 1156 982 804 435 371 336 253
110 99 98 47
BmgBI CAC'GTC
Cuts at: 1318 2464 2869 1318
Size: 1146 405 5713
Fragments arranged by size:
5713 1146 405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3025 4995 5953
Size: 416 53 83 399 24 1608 1970 958
Cuts at: 5953 442
Size: 1753
Fragments arranged by size:
1970 1753 1608 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7264
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 7264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2381 3347 3551 3779 3914 4127 4487
Size: 965 177 966 204 228 135 213 360
Cuts at: 4487 5924 1239
Size: 1437 2579
Fragments arranged by size:
2579 1437 966 965 360 228 213 204
177 135
Bpu10I CC'TnA_GC
Cuts at: 2678 3004 2678
Size: 326 6938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5389 6254 6552 5389
Size: 865 298 6101
Fragments arranged by size:
6101 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3917 5927 882
Size: 617 2418 2010 2219
Fragments arranged by size:
2418 2219 2010 617
BsaAI yAC'GTr
Cuts at: 493 1204 2428 3046 3370 3970 5055 493
Size: 711 1224 618 324 600 1085 2702
Fragments arranged by size:
2702 1224 1085 711 618 600 324
BsaBI GATnn'nnATC
Cuts at: 3986 7258 3986
Size: 3272 3992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2248 2559 3682 5071
Size: 53 83 186 936 715 311 1123 1389
Cuts at: 5071 5453 6088 275
Size: 382 635 1451
Fragments arranged by size:
1451 1389 1123 936 715 635 382 311
186 83 53
BsaWI w'CCGG_w
Cuts at: 3657 4284 5697 6095 6369 6516 6927 3657
Size: 627 1413 398 274 147 411 3994
Fragments arranged by size:
3994 1413 627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4085 4115 1178
Size: 30 165 30 2682 30 4327
Fragments arranged by size:
4327 2682 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2180 2762 2993 3197 3491 3859 3935
Size: 847 147 582 231 204 294 368 76
Cuts at: 3935 4484 4493 4496 4499 1186
Size: 549 9 3 3 3951
Fragments arranged by size:
3951 847 582 549 368 294 231 204
147 76 9 3 3
BseYI C'CCAG_C
Cuts at: 2895 3457 3583 4086 4531 6467 6963 2895
Size: 562 126 503 445 1936 496 3196
Fragments arranged by size:
3196 1936 562 503 496 445 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 2239 2363 1163
Size: 573 477 26 124 6064
Fragments arranged by size:
6064 573 477 124 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2275 4299 4399 4714 5475 6503
Size: 275 186 274 2024 100 315 761 1028
Cuts at: 6503 1540
Size: 2301
Fragments arranged by size:
2301 2024 1028 761 315 275 274 186
100
BsiHKAI G_wGCw'C
Cuts at: 2174 2505 3539 4305 4343 5325 5410 6481 2174
Size: 331 1034 766 38 982 85 1071 2957
Fragments arranged by size:
2957 1071 1034 982 766 331 85 38
BsmI GAATG_Cn'
Cuts at: 3085 4775 4868 3085
Size: 1690 93 5481
Fragments arranged by size:
5481 1690 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2693 4103 2693
Size: 1410 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2126 2174 2379 2477 2505 2912
Size: 399 300 36 48 205 98 28 407
Cuts at: 2912 3022 3121 3492 3539 4305 4343 4583 5325
Size: 110 99 371 47 766 38 240 742
Cuts at: 5325 5410 6481 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 766 742 407 399 371 300
240 205 110 99 98 85 48 47
38 36 28
BspEI T'CCGG_A
Cuts at: 6927 6927
Size: 7264
BspHI T'CATG_A
Cuts at: 2544 3075 3723 5153 6883 2544
Size: 531 648 1430 1730 2925
Fragments arranged by size:
2925 1730 1430 648 531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4559 4619 844
Size: 3715 60 3489
Fragments arranged by size:
3715 3489 60
BsrBI CCG'CTC
Cuts at: 1394 1823 2818 3883 5151 1394
Size: 429 995 1065 1268 3507
Fragments arranged by size:
3507 1268 1065 995 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4528 5759 5933 65
Size: 1183 3280 1231 174 1396
Fragments arranged by size:
3280 1396 1231 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3728 3782 3951 5908 6095 1700
Size: 110 1918 54 169 1957 187 2869
Fragments arranged by size:
2869 1957 1918 187 169 110 54
BsrGI T'GTAC_A
Cuts at: 96 4350 96
Size: 4254 3010
BssHII G'CGCG_C
Cuts at: 1925 3416 1925
Size: 1491 5773
BssSI C'ACGA_G
Cuts at: 4208 5324 6336 4208
Size: 1116 1012 5136
Fragments arranged by size:
5136 1116 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2200 794
Size: 1406 5858
BstBI TT'CG_AA
Cuts at: 6068 6068
Size: 7264
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7264
BstXI CCAn_nnnn'nTGG
Cuts at: 2072 2446 4009 2072
Size: 374 1563 5327
Fragments arranged by size:
5327 1563 374
BstYI r'GATC_y
Cuts at: 1972 2667 3390 3606 4380 4594 4961 5346 5363
Size: 695 723 216 774 214 367 385 17
Cuts at: 5363 6815 6901 6913 7259 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 774 723 695 385 367 346
216 214 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2103 2707 2826 2968 3685 3771 3808
Size: 552 1038 604 119 142 717 86 37
Cuts at: 3808 3862 4002 513
Size: 54 140 3775
Fragments arranged by size:
3775 1038 717 604 552 142 140 119
86 54 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3328 3459 3973 3982 4049 487
Size: 683 56 2102 131 514 9 67 3702
Fragments arranged by size:
3702 2102 683 514 131 67 56 9
BtsI GCAGTG_nn'
Cuts at: 2586 4780 5574 5594 2586
Size: 2194 794 20 4256
Fragments arranged by size:
4256 2194 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7264
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3027 3062 3481 3516 529
Size: 35 2463 35 419 35 4277
Fragments arranged by size:
4277 2463 419 35 35 35
DraI TTT'AAA
Cuts at: 4568 4568
Size: 7264
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2414 6271 809
Size: 507 1098 3857 1802
Fragments arranged by size:
3857 1802 1098 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2313 2322
Size: 54 1425 50 275 18 270 213 9
Cuts at: 2322 2466 2532 2710 2730 2926 3024 4134 4296
Size: 144 66 178 20 196 98 1110 162
Cuts at: 4296 4711 5600 7229 8
Size: 415 889 1629 43
Fragments arranged by size:
1629 1425 1110 889 415 275 270 213
196 178 162 144 98 66 54 50
43 20 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4296 4711 1537
Size: 275 2484 415 4090
Fragments arranged by size:
4090 2484 415 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 3734 4022 4076 4371 5194 1353
Size: 938 1443 288 54 295 823 3423
Fragments arranged by size:
3423 1443 938 823 295 288 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5851 6225 6371 387
Size: 1064 138 4262 374 146 1280
Fragments arranged by size:
4262 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2503 4303 2503
Size: 1800 5464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2381 2467 2684 3347 3491 3551
Size: 393 572 177 86 217 663 144 60
Cuts at: 3551 3758 3779 3914 4127 4487 5327 5924 6711
Size: 207 21 135 213 360 840 597 787
Cuts at: 6711 6979 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 663 597 572 393 360
268 217 213 207 177 144 135 86
60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2156 2264 3425 3473 2015
Size: 141 108 1161 48 5806
Fragments arranged by size:
5806 1161 141 108 48
EcoO109I rG'GnC_Cy
Cuts at: 2149 2239 3019 3117 3118 3488 3619 2149
Size: 90 780 98 1 370 131 5794
Fragments arranged by size:
5794 780 370 131 98 90 1
EcoRI G'AATT_C
Cuts at: 3512 4573 4951 3512
Size: 1061 378 5825
Fragments arranged by size:
5825 1061 378
FspI TGC'GCA
Cuts at: 5770 5770
Size: 7264
HaeII r_GCGC'y
Cuts at: 1678 2180 2251 3685 4433 6411 1678
Size: 502 71 1434 748 1978 2531
Fragments arranged by size:
2531 1978 1434 748 502 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2858 2890 4085 4117 5905
Size: 32 135 32 1286 32 1195 32 1788
Cuts at: 5905 5937 5979 6011 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1788 1286 1195 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 4608 4854 669
Size: 988 281 1234 1436 246 3079
Fragments arranged by size:
3079 1436 1234 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7264
HpaI GTT'AAC
Cuts at: 4854 4854
Size: 7264
KasI G'GCGC_C
Cuts at: 2247 3681 2247
Size: 1434 5830
KpnI G_GTAC'C
Cuts at: 3658 4589 3658
Size: 931 6333
MfeI C'AATT_G
Cuts at: 4863 4863
Size: 7264
MluI A'CGCG_T
Cuts at: 7140 7140
Size: 7264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6378 6562 1305
Size: 5073 184 2007
Fragments arranged by size:
5073 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2324 2534 3026 4136 10
Size: 54 1425 343 492 210 492 1110 3138
Fragments arranged by size:
3138 1425 1110 492 492 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2579 3569 4136 4142 4274 4346
Size: 648 1050 363 990 567 6 132 72
Cuts at: 4346 5222 5581 5740 518
Size: 876 359 159 2042
Fragments arranged by size:
2042 1050 990 876 648 567 363 359
159 132 72 6
MspA1I CmG'CkG
Cuts at: 1606 2105 2277 2709 3030 3089 3810 4680 5357
Size: 499 172 432 321 59 721 870 677
Cuts at: 5357 6505 6750 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 870 721 677 499 432 321
245 172 59
NaeI GCC'GGC
Cuts at: 3784 3784
Size: 7264
NarI GG'CG_CC
Cuts at: 2248 3682 2248
Size: 1434 5830
NcoI C'CATG_G
Cuts at: 513 1065 3771 513
Size: 552 2706 4006
Fragments arranged by size:
4006 2706 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7264
NgoMIV G'CCGG_C
Cuts at: 3782 3782
Size: 7264
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7264
NotI GC'GGCC_GC
Cuts at: 4711 4711
Size: 7264
NspI r_CATG'y
Cuts at: 1132 1229 4622 6167 1132
Size: 97 3393 1545 2229
Fragments arranged by size:
3393 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4179 1348
Size: 2831 4433
PciI A'CATG_T
Cuts at: 6163 6163
Size: 7264
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3982 4179 1108
Size: 2874 197 4193
Fragments arranged by size:
4193 2874 197
PfoI T'CCnGG_A
Cuts at: 2145 2358 2835 2145
Size: 213 477 6574
Fragments arranged by size:
6574 477 213
PmeI GTTT'AAAC
Cuts at: 4568 4568
Size: 7264
PmlI CAC'GTG
Cuts at: 2428 3370 3970 2428
Size: 942 600 5722
Fragments arranged by size:
5722 942 600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 5333 5365 6846 6878 2756
Size: 32 2545 32 1481 32 3142
Fragments arranged by size:
3142 2545 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2239 3619 2149
Size: 90 1380 5794
Fragments arranged by size:
5794 1380 90
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7264
PsiI TTA'TAA
Cuts at: 4834 4834
Size: 7264
PspOMI G'GGCC_C
Cuts at: 2908 3018 3117 3488 2908
Size: 110 99 371 6684
Fragments arranged by size:
6684 371 110 99
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4343 4375 4343
Size: 32 7232
PstI C_TGCA'G
Cuts at: 830 2268 2282 4407 4616 830
Size: 1438 14 2125 209 3478
Fragments arranged by size:
3478 2125 1438 209 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7264
SacI G_AGCT'C
Cuts at: 2505 4305 2505
Size: 1800 5464
SacII CC_GC'GG
Cuts at: 2106 2710 3811 2106
Size: 604 1101 5559
Fragments arranged by size:
5559 1101 604
SalI G'TCGA_C
Cuts at: 1936 4606 1936
Size: 2670 4594
SapI GCTCTTCn'nnn_
Cuts at: 3734 4076 3734
Size: 342 6922
SbfI CC_TGCA'GG
Cuts at: 2268 4616 2268
Size: 2348 4916
ScaI AGT'ACT
Cuts at: 2069 5512 7224 2069
Size: 3443 1712 2109
Fragments arranged by size:
3443 2109 1712
SexAI A'CCwGG_T
Cuts at: 3549 3549
Size: 7264
SfcI C'TryA_G
Cuts at: 826 1045 2264 2278 4403 4612 5747 6428 6619
Size: 219 1219 14 2125 209 1135 681 191
Cuts at: 6619 826
Size: 1471
Fragments arranged by size:
2125 1471 1219 1135 681 219 209 191
14
SfiI GGCCn_nnn'nGGCC
Cuts at: 2321 2321
Size: 7264
SfoI GGC'GCC
Cuts at: 2249 3683 2249
Size: 1434 5830
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7264
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7264
SmaI CCC'GGG
Cuts at: 1872 4591 1872
Size: 2719 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5368 6269 6531 820
Size: 197 926 3425 901 262 1553
Fragments arranged by size:
3425 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5055 493
Size: 4562 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7264
SphI G_CATG'C
Cuts at: 1132 4622 1132
Size: 3490 3774
SspI AAT'ATT
Cuts at: 5 52 5188 5
Size: 47 5136 2081
Fragments arranged by size:
5136 2081 47
StuI AGG'CCT
Cuts at: 1517 2783 3764 1517
Size: 1266 981 5017
Fragments arranged by size:
5017 1266 981
StyI C'CwwG_G
Cuts at: 513 1065 2847 3771 3849 4703 513
Size: 552 1782 924 78 854 3074
Fragments arranged by size:
3074 1782 924 854 552 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2554 4078 4413 4552 5291 5308 5461
Size: 1255 974 1524 335 139 739 17 153
Cuts at: 5461 5646 6091 325
Size: 185 445 1498
Fragments arranged by size:
1524 1498 1255 974 739 445 335 185
153 139 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 3573 4017
Size: 274 80 33 51 1443 90 1506 444
Cuts at: 4017 4350 5510 7222 96
Size: 333 1160 1712 138
Fragments arranged by size:
1712 1506 1443 1160 444 333 274 138
90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7224 7256 7224
Size: 32 7232
Tth111I GACn'n_nGTC
Cuts at: 1316 2354 3356 3551 1316
Size: 1038 1002 195 5029
Fragments arranged by size:
5029 1038 1002 195
XbaI T'CTAG_A
Cuts at: 4600 4600
Size: 7264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3418 3896 4276 4315 3418
Size: 478 380 39 6367
Fragments arranged by size:
6367 478 380 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7264
XmaI C'CCGG_G
Cuts at: 1870 4589 1870
Size: 2719 4545
XmnI GAAnn'nnTTC
Cuts at: 5393 5393
Size: 7264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2560 5072 276
Size: 53 83 186 936 1026 2512 2468
Fragments arranged by size:
2512 2468 1026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PvuII RsrII SanDI SrfI SwaI