Restriction map for
pFN21AE4583
(Circular) (Six-base) MAPSORT of: pFN21AE4583.seq Check: 3446 from: 1 to: 7225
pFN21AE4583 7225 bp
With 182 enzymes: SgfI *
March 28, 2012 14:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5035 278
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3465 4568 1937
Size: 1528 1103 4594
Fragments arranged by size:
4594 1528 1103
Acc65I G'GTAC_C
Cuts at: 4546 4546
Size: 7225
AclI AA'CG_TT
Cuts at: 1563 5352 5725 1563
Size: 3789 373 3063
Fragments arranged by size:
3789 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 4114 4407 5288 6672 6940 1632
Size: 476 2006 293 881 1384 268 1917
Fragments arranged by size:
2006 1917 1384 881 476 293 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7028
AflIII A'CryG_T
Cuts at: 1203 2089 4029 6124 7101 1203
Size: 886 1940 2095 977 1327
Fragments arranged by size:
2095 1940 1327 977 886
AgeI A'CCGG_T
Cuts at: 6056 6056
Size: 7225
AhdI GACnn_n'nnGTC
Cuts at: 5954 5954
Size: 7225
AleI CACnn'nnGTG
Cuts at: 1166 4176 1166
Size: 3010 4215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7225
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2409 2441 2409
Size: 32 7193
AlwNI CAG_nnn'CTG
Cuts at: 2829 4114 4968 6540 2829
Size: 1285 854 1572 3514
Fragments arranged by size:
3514 1572 1285 854
ApaLI G'TGCA_C
Cuts at: 5282 6438 5282
Size: 1156 6069
ApoI r'AATT_y
Cuts at: 1493 2240 2519 2577 2923 2998 4534 4760 4912
Size: 747 279 58 346 75 1536 226 152
Cuts at: 4912 7021 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1536 747 346 279 226 152
75 58
AseI AT'TA_AT
Cuts at: 160 1032 5779 160
Size: 872 4747 1606
Fragments arranged by size:
4747 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
AvaI C'yCGr_G
Cuts at: 1870 1943 4550 1870
Size: 73 2607 4545
Fragments arranged by size:
4545 2607 73
BamHI G'GATC_C
Cuts at: 3321 4555 4922 3321
Size: 1234 367 5624
Fragments arranged by size:
5624 1234 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3452 4546 6001 618
Size: 325 217 627 1665 1094 1455 1842
Fragments arranged by size:
1842 1665 1455 1094 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2113 2894 3418 3431 3803 4015 4412 4483
Size: 722 781 524 13 372 212 397 71
Cuts at: 4483 4544 1391
Size: 61 4072
Fragments arranged by size:
4072 781 722 524 397 372 212 71
61 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3486 3735 4272 928
Size: 971 1587 249 537 3881
Fragments arranged by size:
3881 1587 971 537 249
BbvCI CC'TCA_GC
Cuts at: 3163 3163
Size: 7225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5416 5450 1903
Size: 34 3479 34 3678
Fragments arranged by size:
3678 3479 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5117 6327 5117
Size: 1210 6015
BclI T'GATC_A
Cuts at: 1548 2037 1548
Size: 489 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3228 5836 136
Size: 107 122 71 631 605 1556 2608 1525
Fragments arranged by size:
2608 1556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2566 7220 2566
Size: 4654 2571
BlpI GC'TnA_GC
Cuts at: 4121 4642 4121
Size: 521 6704
Bme1580I G_kGCm'C
Cuts at: 1790 3189 4498 5286 6442 1790
Size: 1399 1309 788 1156 2573
Fragments arranged by size:
2573 1399 1309 1156 788
BmgBI CAC'GTC
Cuts at: 1318 3097 4030 4504 1318
Size: 1779 933 474 4039
Fragments arranged by size:
4039 1779 933 474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2582 3953 4956
Size: 416 53 83 399 24 1165 1371 1003
Cuts at: 4956 5914 442
Size: 958 1753
Fragments arranged by size:
1753 1371 1165 1003 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2675 1056
Size: 1619 5606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4502
Size: 7225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 3085 3942 5885 1239
Size: 896 950 857 1943 2579
Fragments arranged by size:
2579 1943 950 896 857
Bpu10I CC'TnA_GC
Cuts at: 3109 3163 3671 3109
Size: 54 508 6663
Fragments arranged by size:
6663 508 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4195 4381 5350 6215 6513 4195
Size: 186 969 865 298 4907
Fragments arranged by size:
4907 969 865 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4131 4292 5888 882
Size: 617 2632 161 1596 2219
Fragments arranged by size:
2632 2219 1596 617 161
BsaAI yAC'GTr
Cuts at: 493 1204 2092 3970 4020 5016 493
Size: 711 888 1878 50 996 2702
Fragments arranged by size:
2702 1878 996 888 711 50
BsaBI GATnn'nnATC
Cuts at: 2181 7219 2181
Size: 5038 2187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5032 5414 6049 275
Size: 53 83 186 936 3499 382 635 1451
Fragments arranged by size:
3499 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5658 6056 6330 6477 6888 5658
Size: 398 274 147 411 5995
Fragments arranged by size:
5995 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2487 2517 1178
Size: 30 165 30 1084 30 5886
Fragments arranged by size:
5886 1084 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2511 2994 1186
Size: 1325 483 5417
Fragments arranged by size:
5417 1325 483
BseYI C'CCAG_C
Cuts at: 3150 3665 3826 6428 6924 3150
Size: 515 161 2602 496 3451
Fragments arranged by size:
3451 2602 515 496 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4154 1163
Size: 573 2418 4234
Fragments arranged by size:
4234 2418 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4273 4675 5436 6464 1540
Size: 275 186 2272 402 761 1028 2301
Fragments arranged by size:
2301 2272 1028 761 402 275 186
BsiHKAI G_wGCw'C
Cuts at: 2113 2264 3418 4412 5286 5371 6442 2113
Size: 151 1154 994 874 85 1071 2896
Fragments arranged by size:
2896 1154 1071 994 874 151 85
BsiWI C'GTAC_G
Cuts at: 4020 4020
Size: 7225
BsmI GAATG_Cn'
Cuts at: 3820 4043 4736 4829 3820
Size: 223 693 93 6216
Fragments arranged by size:
6216 693 223 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3103 3103
Size: 7225
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2264 2894 3189 3418 3431 3803
Size: 399 323 151 630 295 229 13 372
Cuts at: 3803 4015 4412 4483 4498 4544 5286 5371 6442
Size: 212 397 71 15 46 742 85 1071
Cuts at: 6442 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 630 399 397 372 323
295 229 212 151 85 71 46 15
13
BspEI T'CCGG_A
Cuts at: 6888 6888
Size: 7225
BspHI T'CATG_A
Cuts at: 5114 6844 5114
Size: 1730 5495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2844 4580 844
Size: 2000 1736 3489
Fragments arranged by size:
3489 2000 1736
BsrBI CCG'CTC
Cuts at: 1394 1823 5112 1394
Size: 429 3289 3507
Fragments arranged by size:
3507 3289 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2856 3162 3274 3939 4452 5720 5894
Size: 1183 1608 306 112 665 513 1268 174
Cuts at: 5894 65
Size: 1396
Fragments arranged by size:
1608 1396 1268 1183 665 513 306 174
112
BsrFI r'CCGG_y
Cuts at: 1700 1810 5869 6056 1700
Size: 110 4059 187 2869
Fragments arranged by size:
4059 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7225
BssSI C'ACGA_G
Cuts at: 3197 5285 6297 3197
Size: 2088 1012 4125
Fragments arranged by size:
4125 2088 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4114 794
Size: 3320 3905
BstBI TT'CG_AA
Cuts at: 6029 6029
Size: 7225
BstEII G'GTnAC_C
Cuts at: 1184 3191 1184
Size: 2007 5218
BstXI CCAn_nnnn'nTGG
Cuts at: 3826 3826
Size: 7225
BstYI r'GATC_y
Cuts at: 1972 2566 3321 4555 4922 5307 5324 6776 6862
Size: 594 755 1234 367 385 17 1452 86
Cuts at: 6862 6874 7220 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1234 755 594 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2429 3343 4226 2429
Size: 914 883 5428
Fragments arranged by size:
5428 914 883
BtgI C'CryG_G
Cuts at: 513 1065 2436 3408 513
Size: 552 1371 972 4330
Fragments arranged by size:
4330 1371 972 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6486
Fragments arranged by size:
6486 683 56
BtsI GCAGTG_nn'
Cuts at: 4741 5535 5555 4741
Size: 794 20 6411
Fragments arranged by size:
6411 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7190
DraI TTT'AAA
Cuts at: 4529 4529
Size: 7225
DraIII CAC_nnn'GTG
Cuts at: 2075 3620 2075
Size: 1545 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6232 809
Size: 507 4916 1802
Fragments arranged by size:
4916 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2869 3229 3405
Size: 54 1425 50 275 18 1039 360 176
Cuts at: 3405 3898 3964 4270 4672 5561 7190 8
Size: 493 66 306 402 889 1629 43
Fragments arranged by size:
1629 1425 1039 889 493 402 360 306
275 176 66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4270 4672 1537
Size: 275 2458 402 4090
Fragments arranged by size:
4090 2458 402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5155 1353
Size: 3802 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4086 5812 6186 6332 387
Size: 1064 138 2497 1726 374 146 1280
Fragments arranged by size:
2497 1726 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2111 3416 4410 2111
Size: 1305 994 4926
Fragments arranged by size:
4926 1305 994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2135 3085 3942 4114 4407 5288
Size: 393 476 27 950 857 172 293 881
Cuts at: 5288 5885 6672 6940 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 950 881 857 787 597 476 393
293 268 172 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3176 3305 3176
Size: 129 7096
EcoRI G'AATT_C
Cuts at: 4534 4912 4534
Size: 378 6847
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 4488 2444
Size: 2044 5181
FspI TGC'GCA
Cuts at: 5731 5731
Size: 7225
HaeII r_GCGC'y
Cuts at: 1678 6372 1678
Size: 4694 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3005 3037 4016 4048 4229
Size: 32 135 32 1433 32 979 32 181
Cuts at: 4229 4261 5866 5898 5940 5972 1373
Size: 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 1433 979 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2953 3292 4569 4815 669
Size: 988 281 208 807 339 1277 246 3079
Fragments arranged by size:
3079 1277 988 807 339 281 246 208
HindIII A'AGCT_T
Cuts at: 748 2800 748
Size: 2052 5173
HpaI GTT'AAC
Cuts at: 2146 4815 2146
Size: 2669 4556
KpnI G_GTAC'C
Cuts at: 4550 4550
Size: 7225
MfeI C'AATT_G
Cuts at: 4824 4824
Size: 7225
MluI A'CGCG_T
Cuts at: 7101 7101
Size: 7225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2790 3354 3988 6339 6523 1305
Size: 1485 564 634 2351 184 2007
Fragments arranged by size:
2351 2007 1485 634 564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3966 10
Size: 54 1425 343 2134 3269
Fragments arranged by size:
3269 2134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 4082 4176 4421 5183 5542 5701
Size: 648 1009 1907 94 245 762 359 159
Cuts at: 5701 518
Size: 2042
Fragments arranged by size:
2042 1907 1009 762 648 359 245 159
94
MspA1I CmG'CkG
Cuts at: 1606 2397 3167 4134 4641 5318 6466 6711 1606
Size: 791 770 967 507 677 1148 245 2120
Fragments arranged by size:
2120 1148 967 791 770 677 507 245
NcoI C'CATG_G
Cuts at: 513 1065 2436 513
Size: 552 1371 5302
Fragments arranged by size:
5302 1371 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7225
NheI G'CTAG_C
Cuts at: 1052 2671 1052
Size: 1619 5606
NotI GC'GGCC_GC
Cuts at: 4672 4672
Size: 7225
NspI r_CATG'y
Cuts at: 1132 1229 2865 4087 4583 6128 1132
Size: 97 1636 1222 496 1545 2229
Fragments arranged by size:
2229 1636 1545 1222 496 97
PasI CC'CwG_GG
Cuts at: 1348 2325 2887 1348
Size: 977 562 5686
Fragments arranged by size:
5686 977 562
PciI A'CATG_T
Cuts at: 6124 6124
Size: 7225
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7225
PfoI T'CCnGG_A
Cuts at: 3002 3002
Size: 7225
PmeI GTTT'AAAC
Cuts at: 4529 4529
Size: 7225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5294 5326 6807 6839 5294
Size: 32 1481 32 5680
Fragments arranged by size:
5680 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7225
PsiI TTA'TAA
Cuts at: 4795 4795
Size: 7225
PstI C_TGCA'G
Cuts at: 830 3565 4577 830
Size: 2735 1012 3478
Fragments arranged by size:
3478 2735 1012
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7225
PvuII CAG'CTG
Cuts at: 2397 3167 2397
Size: 770 6455
SacI G_AGCT'C
Cuts at: 2113 3418 4412 2113
Size: 1305 994 4926
Fragments arranged by size:
4926 1305 994
SalI G'TCGA_C
Cuts at: 1936 4567 1936
Size: 2631 4594
SbfI CC_TGCA'GG
Cuts at: 4577 4577
Size: 7225
ScaI AGT'ACT
Cuts at: 5473 7185 5473
Size: 1712 5513
SexAI A'CCwGG_T
Cuts at: 3616 3616
Size: 7225
SfcI C'TryA_G
Cuts at: 826 1045 2149 3217 3561 4507 4573 5708 6389
Size: 219 1104 1068 344 946 66 1135 681
Cuts at: 6389 6580 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1104 1068 946 681 344 219
191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7225
SmaI CCC'GGG
Cuts at: 1872 4552 1872
Size: 2680 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4174 4396 5329 6230 6492 820
Size: 197 926 2231 222 933 901 262 1553
Fragments arranged by size:
2231 1553 933 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 4020 5016 493
Size: 3527 996 2702
Fragments arranged by size:
3527 2702 996
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7225
SphI G_CATG'C
Cuts at: 1132 4583 1132
Size: 3451 3774
SspI AAT'ATT
Cuts at: 5 52 2785 5149 5
Size: 47 2733 2364 2081
Fragments arranged by size:
2733 2364 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7225
StyI C'CwwG_G
Cuts at: 513 1065 2436 3363 3865 4066 4306 4431 4513
Size: 552 1371 927 502 201 240 125 82
Cuts at: 4513 4664 513
Size: 151 3074
Fragments arranged by size:
3074 1371 927 552 502 240 201 151
125 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2900 5252 5269 5422 5607 6052 325
Size: 1255 1320 2352 17 153 185 445 1498
Fragments arranged by size:
2352 1498 1320 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5471 7183 96
Size: 274 80 33 51 1443 3494 1712 138
Fragments arranged by size:
3494 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2487 2519 7185 7217 2487
Size: 32 4666 32 2495
Fragments arranged by size:
4666 2495 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7225
XbaI T'CTAG_A
Cuts at: 4561 4561
Size: 7225
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7225
XmaI C'CCGG_G
Cuts at: 1870 4550 1870
Size: 2680 4545
XmnI GAAnn'nnTTC
Cuts at: 2371 3493 4468 5354 2371
Size: 1122 975 886 4242
Fragments arranged by size:
4242 1122 975 886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5033 276
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BstZ17I EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI