Restriction map for
pFN21AE4580
(Circular) (Six-base) MAPSORT of: pFN21AE4580.seq Check: 1486 from: 1 to: 7105
pFN21AE4580 7105 bp
With 182 enzymes: SgfI *
March 28, 2012 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3457 3457
Size: 7105
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4915 278
Size: 53 83 186 936 3379 2468
Fragments arranged by size:
3379 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4448 1937
Size: 2511 4594
Acc65I G'GTAC_C
Cuts at: 4426 4426
Size: 7105
AclI AA'CG_TT
Cuts at: 1563 5232 5605 1563
Size: 3669 373 3063
Fragments arranged by size:
3669 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4292 5168 6552 6820 1632
Size: 2660 876 1384 268 1917
Fragments arranged by size:
2660 1917 1384 876 268
AfeI AGC'GCT
Cuts at: 3773 3773
Size: 7105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6908
AflIII A'CryG_T
Cuts at: 1203 6004 6981 1203
Size: 4801 977 1327
Fragments arranged by size:
4801 1327 977
AgeI A'CCGG_T
Cuts at: 5936 5936
Size: 7105
AhdI GACnn_n'nnGTC
Cuts at: 5834 5834
Size: 7105
AleI CACnn'nnGTG
Cuts at: 1166 2127 2711 3404 1166
Size: 961 584 693 4867
Fragments arranged by size:
4867 961 693 584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7105
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2708 2740 2708
Size: 32 7073
AlwNI CAG_nnn'CTG
Cuts at: 2746 3139 3385 3472 3556 4848 6420 2746
Size: 393 246 87 84 1292 1572 3431
Fragments arranged by size:
3431 1572 1292 393 246 87 84
ApaI G_GGCC'C
Cuts at: 3156 3156
Size: 7105
ApaLI G'TGCA_C
Cuts at: 5162 6318 5162
Size: 1156 5949
ApoI r'AATT_y
Cuts at: 1493 4414 4640 4792 6901 1493
Size: 2921 226 152 2109 1697
Fragments arranged by size:
2921 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5659 160
Size: 872 4627 1606
Fragments arranged by size:
4627 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
AvaI C'yCGr_G
Cuts at: 1870 1943 2757 3281 3402 3638 3981 4430 1870
Size: 73 814 524 121 236 343 449 4545
Fragments arranged by size:
4545 814 524 449 343 236 121 73
AvrII C'CTAG_G
Cuts at: 2572 2572
Size: 7105
BamHI G'GATC_C
Cuts at: 3634 4435 4802 3634
Size: 801 367 5937
Fragments arranged by size:
5937 801 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2075 2097 2438 4304
Size: 325 217 627 279 9 22 341 1866
Cuts at: 4304 4426 5881 618
Size: 122 1455 1842
Fragments arranged by size:
1866 1842 1455 627 341 325 279 217
122 22 9
BanII G_rGCy'C
Cuts at: 1391 2268 2454 3156 3448 3747 4217 4286 4424
Size: 877 186 702 292 299 470 69 138
Cuts at: 4424 1391
Size: 4072
Fragments arranged by size:
4072 877 702 470 299 292 186 138
69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4235 4349 928
Size: 971 2336 114 3684
Fragments arranged by size:
3684 2336 971 114
BbvCI CC'TCA_GC
Cuts at: 2220 3378 4012 2220
Size: 1158 634 5313
Fragments arranged by size:
5313 1158 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2300 2334 2975 3009 5296 5330 1903
Size: 34 363 34 641 34 2287 34 3678
Fragments arranged by size:
3678 2287 641 363 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4997 6207 4997
Size: 1210 5895
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7105
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2095 3615 5716
Size: 107 122 71 631 605 423 1520 2101
Cuts at: 5716 136
Size: 1525
Fragments arranged by size:
2101 1525 1520 631 605 423 122 107
71
BglII A'GATC_T
Cuts at: 7100 7100
Size: 7105
BlpI GC'TnA_GC
Cuts at: 3775 4361 4522 3775
Size: 586 161 6358
Fragments arranged by size:
6358 586 161
Bme1580I G_kGCm'C
Cuts at: 1790 2441 2758 3156 4307 5166 6322 1790
Size: 651 317 398 1151 859 1156 2573
Fragments arranged by size:
2573 1156 1151 859 651 398 317
BmgBI CAC'GTC
Cuts at: 1318 2866 3672 1318
Size: 1548 806 4751
Fragments arranged by size:
4751 1548 806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4836 5794 442
Size: 416 53 83 399 24 3419 958 1753
Fragments arranged by size:
3419 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3790 3790
Size: 7105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2696 3119 3494 3515 3533 3620 3841 3977
Size: 1457 423 375 21 18 87 221 136
Cuts at: 3977 4270 4304 5765 1239
Size: 293 34 1461 2579
Fragments arranged by size:
2579 1461 1457 423 375 293 221 136
87 34 21 18
Bpu10I CC'TnA_GC
Cuts at: 2220 2338 3140 3378 4012 4216 2220
Size: 118 802 238 634 204 5109
Fragments arranged by size:
5109 802 634 238 204 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 5230 6095 6393 2816
Size: 2414 865 298 3528
Fragments arranged by size:
3528 2414 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3953 5768 882
Size: 617 2454 1815 2219
Fragments arranged by size:
2454 2219 1815 617
BsaAI yAC'GTr
Cuts at: 493 1204 4141 4896 493
Size: 711 2937 755 2702
Fragments arranged by size:
2937 2702 755 711
BsaBI GATnn'nnATC
Cuts at: 3633 4043 7099 3633
Size: 410 3056 3639
Fragments arranged by size:
3639 3056 410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 4912 5294 5929
Size: 53 83 186 936 1990 1389 382 635
Cuts at: 5929 275
Size: 1451
Fragments arranged by size:
1990 1451 1389 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2124 5538 5936 6210 6357 6768 2124
Size: 3414 398 274 147 411 2461
Fragments arranged by size:
3414 2461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2708 2738 3773 3803 1178
Size: 30 165 30 1305 30 1035 30 4480
Fragments arranged by size:
4480 1305 1035 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2341 2381 2462 2513 2635 2693 2732 2735
Size: 1155 40 81 51 122 58 39 3
Cuts at: 2735 2870 2873 3116 3200 3371 3374 3446 3491
Size: 135 3 243 84 171 3 72 45
Cuts at: 3491 3638 3746 3755 3782 3821 3857 4274 1186
Size: 147 108 9 27 39 36 417 4017
Fragments arranged by size:
4017 1155 417 243 171 147 135 122
108 84 81 72 58 51 45 40
39 39 36 27 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2554 3618 6308 6804 2554
Size: 1064 2690 496 2855
Fragments arranged by size:
2855 2690 1064 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3989 1163
Size: 573 2253 4279
Fragments arranged by size:
4279 2253 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 2064 3903 4555 5316 6344
Size: 275 186 45 18 1839 652 761 1028
Cuts at: 6344 1540
Size: 2301
Fragments arranged by size:
2301 1839 1028 761 652 275 186 45
18
BsiHKAI G_wGCw'C
Cuts at: 3146 3448 3747 3827 4286 4362 5166 5251 6322
Size: 302 299 80 459 76 804 85 1071
Cuts at: 6322 3146
Size: 3929
Fragments arranged by size:
3929 1071 804 459 302 299 85 80
76
BsmI GAATG_Cn'
Cuts at: 4616 4709 4616
Size: 93 7012
BsmBI CGTCTCn'nnnn_
Cuts at: 4142 4142
Size: 7105
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2441 2454 2758 3146 3156 3448
Size: 399 478 173 13 304 388 10 292
Cuts at: 3448 3747 3827 4217 4286 4307 4362 4424 5166
Size: 299 80 390 69 21 55 62 742
Cuts at: 5166 5251 6322 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 390 388 304
299 292 173 85 80 69 62 55
21 13 10
BspEI T'CCGG_A
Cuts at: 6768 6768
Size: 7105
BspHI T'CATG_A
Cuts at: 4994 6724 4994
Size: 1730 5375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2772 2952 3457 4460 844
Size: 1928 180 505 1003 3489
Fragments arranged by size:
3489 1928 1003 505 180
BsrBI CCG'CTC
Cuts at: 1394 1823 2199 2275 2899 3037 3091 3643 4237
Size: 429 376 76 624 138 54 552 594
Cuts at: 4237 4992 1394
Size: 755 3507
Fragments arranged by size:
3507 755 624 594 552 429 376 138
76 54
BsrDI GCAATG_nn'
Cuts at: 65 1248 5600 5774 65
Size: 1183 4352 174 1396
Fragments arranged by size:
4352 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2063 2625 3525 5749 5936 1700
Size: 110 253 562 900 2224 187 2869
Fragments arranged by size:
2869 2224 900 562 253 187 110
BsrGI T'GTAC_A
Cuts at: 96 2748 96
Size: 2652 4453
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7105
BssSI C'ACGA_G
Cuts at: 5165 6177 5165
Size: 1012 6093
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7105
BstBI TT'CG_AA
Cuts at: 5909 5909
Size: 7105
BstEII G'GTnAC_C
Cuts at: 1184 3193 1184
Size: 2009 5096
BstXI CCAn_nnnn'nTGG
Cuts at: 2674 2696 3886 2674
Size: 22 1190 5893
Fragments arranged by size:
5893 1190 22
BstYI r'GATC_y
Cuts at: 1972 3105 3634 3699 4435 4802 5187 5204 6656
Size: 1133 529 65 736 367 385 17 1452
Cuts at: 6656 6742 6754 7100 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1133 736 529 385 367 346
86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 3183 3183
Size: 7105
BtgI C'CryG_G
Cuts at: 513 1065 2004 2093 2778 4204 4308 513
Size: 552 939 89 685 1426 104 3310
Fragments arranged by size:
3310 1426 939 685 552 104 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2009 2313 2358 487
Size: 683 56 783 304 45 5234
Fragments arranged by size:
5234 783 683 304 56 45
BtsI GCAGTG_nn'
Cuts at: 2143 2601 4621 5415 5435 2143
Size: 458 2020 794 20 3813
Fragments arranged by size:
3813 2020 794 458 20
ClaI AT'CG_AT
Cuts at: 1554 4047 1554
Size: 2493 4612
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7070
DraI TTT'AAA
Cuts at: 3202 4409 3202
Size: 1207 5898
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6112 809
Size: 507 4796 1802
Fragments arranged by size:
4796 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2061 3038
Size: 54 1425 50 275 18 213 18 977
Cuts at: 3038 4041 4552 5441 7070 8
Size: 1003 511 889 1629 43
Fragments arranged by size:
1629 1425 1003 977 889 511 275 213
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 2061 4552 1537
Size: 275 231 18 2491 4090
Fragments arranged by size:
4090 2491 275 231 18
EarI CTCTTCn'nnn_
Cuts at: 1353 2654 3015 5035 1353
Size: 1301 361 2020 3423
Fragments arranged by size:
3423 2020 1301 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3503 4255 5692 6066 6212 387
Size: 1064 138 1914 752 1437 374 146 1280
Fragments arranged by size:
1914 1437 1280 1064 752 374 146 138
EcoICRI GAG'CTC
Cuts at: 3446 3745 4284 3446
Size: 299 539 6267
Fragments arranged by size:
6267 539 299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2696 3119 3494 3515 3533 3620 3841
Size: 393 1064 423 375 21 18 87 221
Cuts at: 3841 3977 4270 4292 4304 5168 5765 6552 6820
Size: 136 293 22 12 864 597 787 268
Cuts at: 6820 1239
Size: 1524
Fragments arranged by size:
1524 1064 864 787 597 423 393 375
293 268 221 136 87 22 21 18
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 3839 3890 4319 2576
Size: 1263 51 429 5362
Fragments arranged by size:
5362 1263 429 51
EcoO109I rG'GnC_Cy
Cuts at: 2647 2743 2761 2770 3152 3153 3459 3991 2647
Size: 96 18 9 382 1 306 532 5761
Fragments arranged by size:
5761 532 382 306 96 18 9 1
EcoRI G'AATT_C
Cuts at: 4414 4792 4414
Size: 378 6727
FspI TGC'GCA
Cuts at: 5611 5611
Size: 7105
HaeII r_GCGC'y
Cuts at: 1678 2539 3775 4268 6252 1678
Size: 861 1236 493 1984 2531
Fragments arranged by size:
2531 1984 1236 861 493
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2336 2368 5746 5778 5820
Size: 32 135 32 764 32 3378 32 42
Cuts at: 5820 5852 1373
Size: 32 2626
Fragments arranged by size:
3378 2626 764 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4449 4695 669
Size: 988 281 2511 246 3079
Fragments arranged by size:
3079 2511 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7105
HpaI GTT'AAC
Cuts at: 4695 4695
Size: 7105
KpnI G_GTAC'C
Cuts at: 4430 4430
Size: 7105
MfeI C'AATT_G
Cuts at: 4704 4704
Size: 7105
MluI A'CGCG_T
Cuts at: 6981 6981
Size: 7105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3372 6219 6403 1305
Size: 2067 2847 184 2007
Fragments arranged by size:
2847 2067 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4043 10
Size: 54 1425 343 2211 3072
Fragments arranged by size:
3072 2211 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 2711 3404 5063 5422 5581 518
Size: 648 961 584 693 1659 359 159 2042
Fragments arranged by size:
2042 1659 961 693 648 584 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2318 2462 2522 2632 3511 3556 3613
Size: 489 223 144 60 110 879 45 57
Cuts at: 3613 3923 4189 4365 4371 4521 5198 6346 6591
Size: 310 266 176 6 150 677 1148 245
Cuts at: 6591 1606
Size: 2120
Fragments arranged by size:
2120 1148 879 677 489 310 266 245
223 176 150 144 110 60 57 45
6
NaeI GCC'GGC
Cuts at: 2065 2627 3527 2065
Size: 562 900 5643
Fragments arranged by size:
5643 900 562
NcoI C'CATG_G
Cuts at: 513 1065 2004 4204 513
Size: 552 939 2200 3414
Fragments arranged by size:
3414 2200 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7105
NgoMIV G'CCGG_C
Cuts at: 2063 2625 3525 2063
Size: 562 900 5643
Fragments arranged by size:
5643 900 562
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7105
NotI GC'GGCC_GC
Cuts at: 4552 4552
Size: 7105
NruI TCG'CGA
Cuts at: 3729 3729
Size: 7105
NspI r_CATG'y
Cuts at: 1132 1229 2789 4463 6008 1132
Size: 97 1560 1674 1545 2229
Fragments arranged by size:
2229 1674 1560 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4300 1348
Size: 2952 4153
PciI A'CATG_T
Cuts at: 6004 6004
Size: 7105
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2554 1108
Size: 1446 5659
PfoI T'CCnGG_A
Cuts at: 3885 4320 3885
Size: 435 6670
PmeI GTTT'AAAC
Cuts at: 4409 4409
Size: 7105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2740 5174 5206 6687 6719 2708
Size: 32 2434 32 1481 32 3094
Fragments arranged by size:
3094 2434 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2647 2743 2761 3459 2647
Size: 96 18 698 6293
Fragments arranged by size:
6293 698 96 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7105
PsiI TTA'TAA
Cuts at: 4675 4675
Size: 7105
PspOMI G'GGCC_C
Cuts at: 3152 3152
Size: 7105
PstI C_TGCA'G
Cuts at: 830 2608 2637 2769 2853 3129 3504 4457 830
Size: 1778 29 132 84 276 375 953 3478
Fragments arranged by size:
3478 1778 953 375 276 132 84 29
PvuI CG_AT'CG
Cuts at: 2001 3903 2001
Size: 1902 5203
PvuII CAG'CTG
Cuts at: 2462 2522 2632 3511 3556 3923 4189 2462
Size: 60 110 879 45 367 266 5378
Fragments arranged by size:
5378 879 367 266 110 60 45
SacI G_AGCT'C
Cuts at: 3448 3747 4286 3448
Size: 299 539 6267
Fragments arranged by size:
6267 539 299
SacII CC_GC'GG
Cuts at: 2096 2096
Size: 7105
SalI G'TCGA_C
Cuts at: 1936 4447 1936
Size: 2511 4594
SanDI GG'GwC_CC
Cuts at: 3459 3459
Size: 7105
SapI GCTCTTCn'nnn_
Cuts at: 3015 3015
Size: 7105
SbfI CC_TGCA'GG
Cuts at: 2769 4457 2769
Size: 1688 5417
ScaI AGT'ACT
Cuts at: 5353 7065 5353
Size: 1712 5393
SexAI A'CCwGG_T
Cuts at: 2883 2883
Size: 7105
SfcI C'TryA_G
Cuts at: 826 1045 2604 2633 2765 2849 3125 3500 3548
Size: 219 1559 29 132 84 276 375 48
Cuts at: 3548 4453 5588 6269 6460 826
Size: 905 1135 681 191 1471
Fragments arranged by size:
1559 1471 1135 905 681 375 276 219
191 132 84 48 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7105
SmaI CCC'GGG
Cuts at: 1872 2759 3983 4432 1872
Size: 887 1224 449 4545
Fragments arranged by size:
4545 1224 887 449
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2831 5209 6110 6372 820
Size: 197 926 888 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 4896 493
Size: 4403 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7105
SphI G_CATG'C
Cuts at: 1132 2789 4463 1132
Size: 1657 1674 3774
Fragments arranged by size:
3774 1674 1657
SspI AAT'ATT
Cuts at: 5 52 5029 5
Size: 47 4977 2081
Fragments arranged by size:
4977 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7105
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2442 2526 2572 3690 4170
Size: 552 939 402 36 84 46 1118 480
Cuts at: 4170 4204 4401 4544 513
Size: 34 197 143 3074
Fragments arranged by size:
3074 1118 939 552 480 402 197 143
84 46 36 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4183 5132 5149 5302 5487 5932 325
Size: 1255 2603 949 17 153 185 445 1498
Fragments arranged by size:
2603 1498 1255 949 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 4020 4056
Size: 274 80 33 51 1443 771 1272 36
Cuts at: 4056 5351 7063 96
Size: 1295 1712 138
Fragments arranged by size:
1712 1443 1295 1272 771 274 138 80
51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3033 3065 7065 7097 3033
Size: 32 4000 32 3041
Fragments arranged by size:
4000 3041 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3330 1316
Size: 2014 5091
XbaI T'CTAG_A
Cuts at: 4441 4441
Size: 7105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3296 3712 3883 3296
Size: 416 171 6518
Fragments arranged by size:
6518 416 171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7105
XmaI C'CCGG_G
Cuts at: 1870 2757 3981 4430 1870
Size: 887 1224 449 4545
Fragments arranged by size:
4545 1224 887 449
XmnI GAAnn'nnTTC
Cuts at: 5234 5234
Size: 7105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4913 276
Size: 53 83 186 936 3379 2468
Fragments arranged by size:
3379 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NarI NsiI PacI PmlI
PspXI PsrI RsrII SfiI SfoI SrfI SwaI