Restriction map for
pFN21AE4579
(Circular) (Six-base) MAPSORT of: pFN21AE4579.seq Check: 8493 from: 1 to: 7060
pFN21AE4579 7060 bp
With 182 enzymes: SgfI *
March 28, 2012 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2069 2341 3256 2069
Size: 272 915 5873
Fragments arranged by size:
5873 915 272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3297 4870 278
Size: 53 83 186 936 1761 1573 2468
Fragments arranged by size:
2468 1761 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3864 4403 1937
Size: 1927 539 4594
Fragments arranged by size:
4594 1927 539
Acc65I G'GTAC_C
Cuts at: 4381 4381
Size: 7060
AclI AA'CG_TT
Cuts at: 1563 5187 5560 1563
Size: 3624 373 3063
Fragments arranged by size:
3624 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 3112 3164 3355 3590 3656 5123 6507
Size: 632 848 52 191 235 66 1467 1384
Cuts at: 6507 6775 1632
Size: 268 1917
Fragments arranged by size:
1917 1467 1384 848 632 268 235 191
66 52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6863
AflIII A'CryG_T
Cuts at: 1203 5959 6936 1203
Size: 4756 977 1327
Fragments arranged by size:
4756 1327 977
AgeI A'CCGG_T
Cuts at: 5891 5891
Size: 7060
AhdI GACnn_n'nnGTC
Cuts at: 5789 5789
Size: 7060
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2244 1184
Size: 1060 6000
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2568 2600 2568
Size: 32 7028
AlwNI CAG_nnn'CTG
Cuts at: 2407 2736 4803 6375 2407
Size: 329 2067 1572 3092
Fragments arranged by size:
3092 2067 1572 329
ApaI G_GGCC'C
Cuts at: 4226 4226
Size: 7060
ApaLI G'TGCA_C
Cuts at: 2171 5117 6273 2171
Size: 2946 1156 2958
Fragments arranged by size:
2958 2946 1156
ApoI r'AATT_y
Cuts at: 1493 3957 4369 4595 4747 6856 1493
Size: 2464 412 226 152 2109 1697
Fragments arranged by size:
2464 2109 1697 412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5614 160
Size: 872 4582 1606
Fragments arranged by size:
4582 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
AvaI C'yCGr_G
Cuts at: 1870 1943 2488 3756 4385 1870
Size: 73 545 1268 629 4545
Fragments arranged by size:
4545 1268 629 545 73
BamHI G'GATC_C
Cuts at: 3697 4192 4390 4757 3697
Size: 495 198 367 6000
Fragments arranged by size:
6000 495 367 198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2466 3674 4381 5836 618
Size: 325 217 627 679 1208 707 1455 1842
Fragments arranged by size:
1842 1455 1208 707 679 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2489 3757 4226 4379 1391
Size: 697 401 1268 469 153 4072
Fragments arranged by size:
4072 1268 697 469 401 153
BbeI G_GCGC'C
Cuts at: 3678 3678
Size: 7060
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2894 3304 3578 4024 928
Size: 971 995 410 274 446 3964
Fragments arranged by size:
3964 995 971 446 410 274
BbvCI CC'TCA_GC
Cuts at: 3220 3220
Size: 7060
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5251 5285 1903
Size: 34 50 34 3230 34 3678
Fragments arranged by size:
3678 3230 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2786 4952 6162 2786
Size: 2166 1210 3684
Fragments arranged by size:
3684 2166 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7060
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2311 3654 5671
Size: 107 122 71 631 605 639 1343 2017
Cuts at: 5671 136
Size: 1525
Fragments arranged by size:
2017 1525 1343 639 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3435 4353 7055 3435
Size: 918 2702 3440
Fragments arranged by size:
3440 2702 918
BlpI GC'TnA_GC
Cuts at: 4477 4477
Size: 7060
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2175 2289 2316 2724 3191 4226 5121
Size: 360 25 114 27 408 467 1035 895
Cuts at: 5121 6277 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1035 895 467 408 360 114
27 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2544 3181 3645
Size: 416 53 83 399 24 1127 637 464
Cuts at: 3645 4791 5749 442
Size: 1146 958 1753
Fragments arranged by size:
1753 1146 1127 958 637 464 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2348 3433 3542 3575 3599 4283 4297
Size: 995 114 1085 109 33 24 684 14
Cuts at: 4297 5720 1239
Size: 1423 2579
Fragments arranged by size:
2579 1423 1085 995 684 114 109 33
24 14
Bpu10I CC'TnA_GC
Cuts at: 2411 3220 2411
Size: 809 6251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3983 5185 6050 6348 3983
Size: 1202 865 298 4695
Fragments arranged by size:
4695 1202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5723 882
Size: 617 4224 2219
Fragments arranged by size:
4224 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3285 4851 493
Size: 711 2081 1566 2702
Fragments arranged by size:
2702 2081 1566 711
BsaBI GATnn'nnATC
Cuts at: 7054 7054
Size: 7060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3294 3675 4867 5249
Size: 53 83 186 936 1761 381 1192 382
Cuts at: 5249 5884 275
Size: 635 1451
Fragments arranged by size:
1761 1451 1192 936 635 382 381 186
83 53
BsaWI w'CCGG_w
Cuts at: 5493 5891 6165 6312 6723 5493
Size: 398 274 147 411 5830
Fragments arranged by size:
5830 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 3020 3050 3515
Size: 30 165 30 945 30 642 30 465
Cuts at: 3515 3545 1178
Size: 30 4693
Fragments arranged by size:
4693 945 642 465 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3086 3392 4361 1186
Size: 1900 306 969 3885
Fragments arranged by size:
3885 1900 969 306
BseYI C'CCAG_C
Cuts at: 2163 2309 2369 2865 3741 6263 6759 2163
Size: 146 60 496 876 2522 496 2464
Fragments arranged by size:
2522 2464 876 496 496 146 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2721 3181 3363 3879 3902 1163
Size: 573 985 460 182 516 23 4321
Fragments arranged by size:
4321 985 573 516 460 182 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4510 5271 6299 1540
Size: 275 186 2509 761 1028 2301
Fragments arranged by size:
2509 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 5121 5206 6277 2175
Size: 2946 85 1071 2958
Fragments arranged by size:
2958 2946 1071 85
BsmI GAATG_Cn'
Cuts at: 2263 3430 4571 4664 2263
Size: 1167 1141 93 4659
Fragments arranged by size:
4659 1167 1141 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2150 2175 2289 2316 2489 2724
Size: 399 298 62 25 114 27 173 235
Cuts at: 2724 3191 3757 4226 4379 5121 5206 6277 1391
Size: 467 566 469 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 566 469 467 399 298
235 173 153 114 85 62 27 25
BspEI T'CCGG_A
Cuts at: 6723 6723
Size: 7060
BspHI T'CATG_A
Cuts at: 4949 6679 4949
Size: 1730 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2069 2341 2762 3256 3924 4266 4415 844
Size: 1225 272 421 494 668 342 149 3489
Fragments arranged by size:
3489 1225 668 494 421 342 272 149
BsrBI CCG'CTC
Cuts at: 1394 1823 4947 1394
Size: 429 3124 3507
Fragments arranged by size:
3507 3124 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2468 2533 2592 3830 5555 5729 65
Size: 1183 1220 65 59 1238 1725 174 1396
Fragments arranged by size:
1725 1396 1238 1220 1183 174 65 59
BsrFI r'CCGG_y
Cuts at: 1700 1810 2320 5704 5891 1700
Size: 110 510 3384 187 2869
Fragments arranged by size:
3384 2869 510 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7060
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7060
BssSI C'ACGA_G
Cuts at: 3419 5120 6132 3419
Size: 1701 1012 4347
Fragments arranged by size:
4347 1701 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2026 2407 3195 794
Size: 1232 381 788 4659
Fragments arranged by size:
4659 1232 788 381
BstBI TT'CG_AA
Cuts at: 5864 5864
Size: 7060
BstEII G'GTnAC_C
Cuts at: 1184 3352 1184
Size: 2168 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2912 2960 2309
Size: 603 48 6409
Fragments arranged by size:
6409 603 48
BstYI r'GATC_y
Cuts at: 1972 3435 3697 4192 4353 4390 4757 5142 5159
Size: 1463 262 495 161 37 367 385 17
Cuts at: 5159 6611 6697 6709 7055 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1463 1452 495 385 367 346 262
161 86 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2613 3963 2613
Size: 1350 5710
BtgI C'CryG_G
Cuts at: 513 1065 2686 2742 3614 513
Size: 552 1621 56 872 3959
Fragments arranged by size:
3959 1621 872 552 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2710 2752 3751 487
Size: 683 56 1484 42 999 3796
Fragments arranged by size:
3796 1484 999 683 56 42
BtsI GCAGTG_nn'
Cuts at: 2823 3597 4576 5370 5390 2823
Size: 774 979 794 20 4493
Fragments arranged by size:
4493 979 794 774 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7060
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7025
DraI TTT'AAA
Cuts at: 4364 4364
Size: 7060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6067 809
Size: 507 4751 1802
Fragments arranged by size:
4751 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 4507 5396
Size: 54 1425 50 275 18 310 2367 889
Cuts at: 5396 7025 8
Size: 1629 43
Fragments arranged by size:
2367 1629 1425 889 310 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4507 1537
Size: 275 2695 4090
Fragments arranged by size:
4090 2695 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2199 4990 1353
Size: 740 106 2791 3423
Fragments arranged by size:
3423 2791 740 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2240 5647 6021 6167 387
Size: 1064 138 651 3407 374 146 1280
Fragments arranged by size:
3407 1280 1064 651 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2264 2348 3112 3164 3355 3433
Size: 393 602 30 84 764 52 191 78
Cuts at: 3433 3542 3575 3590 3599 3656 4283 4297 5123
Size: 109 33 15 9 57 627 14 826
Cuts at: 5123 5720 6507 6775 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 826 787 764 627 602 597 393
268 191 109 84 78 57 52 33
30 15 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3182 3182
Size: 7060
EcoO109I rG'GnC_Cy
Cuts at: 3175 3216 3526 3762 4222 4223 3175
Size: 41 310 236 460 1 6012
Fragments arranged by size:
6012 460 310 236 41 1
EcoRI G'AATT_C
Cuts at: 4369 4747 4369
Size: 378 6682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 7060
FseI GG_CCGG'CC
Cuts at: 2324 2324
Size: 7060
FspI TGC'GCA
Cuts at: 5566 5566
Size: 7060
HaeII r_GCGC'y
Cuts at: 1678 3233 3678 4302 6207 1678
Size: 1555 445 624 1905 2531
Fragments arranged by size:
2531 1905 1555 624 445
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 2932 2964 4151
Size: 32 135 32 1046 32 282 32 1187
Cuts at: 4151 4183 5701 5733 5775 5807 1373
Size: 32 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 1187 1046 282 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4404 4650 669
Size: 988 281 2466 246 3079
Fragments arranged by size:
3079 2466 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7060
HpaI GTT'AAC
Cuts at: 4650 4650
Size: 7060
KasI G'GCGC_C
Cuts at: 3674 3674
Size: 7060
KpnI G_GTAC'C
Cuts at: 4385 4385
Size: 7060
MfeI C'AATT_G
Cuts at: 4659 4659
Size: 7060
MluI A'CGCG_T
Cuts at: 6936 6936
Size: 7060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6174 6358 1305
Size: 4869 184 2007
Fragments arranged by size:
4869 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2142 10
Size: 54 1425 343 310 4928
Fragments arranged by size:
4928 1425 343 310 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2037 2504 2664 2972 5018 5377 5536
Size: 648 871 467 160 308 2046 359 159
Cuts at: 5536 518
Size: 2042
Fragments arranged by size:
2046 2042 871 648 467 359 308 160
159
MspA1I CmG'CkG
Cuts at: 1606 2859 2865 3224 3242 3832 4476 5153 6301
Size: 1253 6 359 18 590 644 677 1148
Cuts at: 6301 6546 1606
Size: 245 2120
Fragments arranged by size:
2120 1253 1148 677 644 590 359 245
18 6
NaeI GCC'GGC
Cuts at: 2322 2322
Size: 7060
NarI GG'CG_CC
Cuts at: 3675 3675
Size: 7060
NcoI C'CATG_G
Cuts at: 513 1065 2686 513
Size: 552 1621 4887
Fragments arranged by size:
4887 1621 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7060
NgoMIV G'CCGG_C
Cuts at: 2320 2320
Size: 7060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7060
NotI GC'GGCC_GC
Cuts at: 4507 4507
Size: 7060
NspI r_CATG'y
Cuts at: 1132 1229 3085 3587 4054 4418 5963 1132
Size: 97 1856 502 467 364 1545 2229
Fragments arranged by size:
2229 1856 1545 502 467 364 97
PasI CC'CwG_GG
Cuts at: 1348 2047 2103 1348
Size: 699 56 6305
Fragments arranged by size:
6305 699 56
PciI A'CATG_T
Cuts at: 5959 5959
Size: 7060
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3171 3438 1108
Size: 2063 267 4730
Fragments arranged by size:
4730 2063 267
PfoI T'CCnGG_A
Cuts at: 2586 2586
Size: 7060
PmeI GTTT'AAAC
Cuts at: 4364 4364
Size: 7060
PmlI CAC'GTG
Cuts at: 3285 3285
Size: 7060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5129 5161 6642 6674 5129
Size: 32 1481 32 5515
Fragments arranged by size:
5515 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3175 3216 3526 3762 3175
Size: 41 310 236 6473
Fragments arranged by size:
6473 310 236 41
PshAI GACnn'nnGTC
Cuts at: 1613 3446 1613
Size: 1833 5227
PsiI TTA'TAA
Cuts at: 4630 4630
Size: 7060
PspOMI G'GGCC_C
Cuts at: 4222 4222
Size: 7060
PstI C_TGCA'G
Cuts at: 830 2864 3229 3267 4412 830
Size: 2034 365 38 1145 3478
Fragments arranged by size:
3478 2034 1145 365 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7060
PvuII CAG'CTG
Cuts at: 2859 2865 3224 3242 3832 2859
Size: 6 359 18 590 6087
Fragments arranged by size:
6087 590 359 18 6
SalI G'TCGA_C
Cuts at: 1936 4402 1936
Size: 2466 4594
SapI GCTCTTCn'nnn_
Cuts at: 2093 2093
Size: 7060
SbfI CC_TGCA'GG
Cuts at: 4412 4412
Size: 7060
ScaI AGT'ACT
Cuts at: 5308 7020 5308
Size: 1712 5348
SfcI C'TryA_G
Cuts at: 826 1045 2605 2854 2860 3225 3263 3766 4408
Size: 219 1560 249 6 365 38 503 642
Cuts at: 4408 5543 6224 6415 826
Size: 1135 681 191 1471
Fragments arranged by size:
1560 1471 1135 681 642 503 365 249
219 191 38 6
SfoI GGC'GCC
Cuts at: 3676 3676
Size: 7060
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7060
SmaI CCC'GGG
Cuts at: 1872 2490 4387 1872
Size: 618 1897 4545
Fragments arranged by size:
4545 1897 618
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3998 5164 6065 6327 820
Size: 197 926 2055 1166 901 262 1553
Fragments arranged by size:
2055 1553 1166 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4851 493
Size: 4358 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7060
SphI G_CATG'C
Cuts at: 1132 3085 4418 1132
Size: 1953 1333 3774
Fragments arranged by size:
3774 1953 1333
SrfI GCCC'GGGC
Cuts at: 2490 2490
Size: 7060
SspI AAT'ATT
Cuts at: 5 52 4984 5
Size: 47 4932 2081
Fragments arranged by size:
4932 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7060
StyI C'CwwG_G
Cuts at: 513 1065 2232 2686 4143 4499 513
Size: 552 1167 454 1457 356 3074
Fragments arranged by size:
3074 1457 1167 552 454 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2614 3627 5087 5104 5257 5442 5887
Size: 1255 1034 1013 1460 17 153 185 445
Cuts at: 5887 325
Size: 1498
Fragments arranged by size:
1498 1460 1255 1034 1013 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5306 7018 96
Size: 274 80 33 51 1443 3329 1712 138
Fragments arranged by size:
3329 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 7020 7052 3515
Size: 32 3473 32 3523
Fragments arranged by size:
3523 3473 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7060
XbaI T'CTAG_A
Cuts at: 4396 4396
Size: 7060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3061 3061
Size: 7060
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7060
XmaI C'CCGG_G
Cuts at: 1870 2488 4385 1870
Size: 618 1897 4545
Fragments arranged by size:
4545 1897 618
XmnI GAAnn'nnTTC
Cuts at: 3641 5189 3641
Size: 1548 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3295 4868 276
Size: 53 83 186 936 1761 1573 2468
Fragments arranged by size:
2468 1761 1573 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SwaI