Restriction map for
pFN21AE4576
(Circular) (Six-base) MAPSORT of: pFN21AE4576.seq Check: 2698 from: 1 to: 6994
pFN21AE4576 6994 bp
With 182 enzymes: SgfI *
March 28, 2012 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2294 4102 2294
Size: 1808 5186
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4804 278
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 1937
Size: 2400 4594
Acc65I G'GTAC_C
Cuts at: 2513 4315 2513
Size: 1802 5192
AclI AA'CG_TT
Cuts at: 1563 5121 5494 1563
Size: 3558 373 3063
Fragments arranged by size:
3558 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2035 2541 3265 3558 3693 4001 4020 5057
Size: 403 506 724 293 135 308 19 1037
Cuts at: 5057 6441 6709 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1037 724 506 403 308 293
268 135 19
AflII C'TTAA_G
Cuts at: 820 1017 3365 820
Size: 197 2348 4449
Fragments arranged by size:
4449 2348 197
AflIII A'CryG_T
Cuts at: 1203 2319 3796 5893 6870 1203
Size: 1116 1477 2097 977 1327
Fragments arranged by size:
2097 1477 1327 1116 977
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 5723 5723
Size: 6994
AleI CACnn'nnGTG
Cuts at: 1166 3764 1166
Size: 2598 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4081 1184
Size: 2897 4097
AlwNI CAG_nnn'CTG
Cuts at: 2309 3046 3529 4737 6309 2309
Size: 737 483 1208 1572 2994
Fragments arranged by size:
2994 1572 1208 737 483
ApaLI G'TGCA_C
Cuts at: 5051 6207 5051
Size: 1156 5838
ApoI r'AATT_y
Cuts at: 1493 2981 3062 3949 4303 4529 4681 6790 1493
Size: 1488 81 887 354 226 152 2109 1697
Fragments arranged by size:
2109 1697 1488 887 354 226 152 81
AseI AT'TA_AT
Cuts at: 160 1032 5548 160
Size: 872 4516 1606
Fragments arranged by size:
4516 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 4319 1870
Size: 73 124 2252 4545
Fragments arranged by size:
4545 2252 124 73
AvrII C'CTAG_G
Cuts at: 3886 3886
Size: 6994
BamHI G'GATC_C
Cuts at: 2055 2432 4324 4691 2055
Size: 377 1892 367 4358
Fragments arranged by size:
4358 1892 377 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2513 3752 4178 4315 5770
Size: 325 217 627 726 1239 426 137 1455
Cuts at: 5770 618
Size: 1842
Fragments arranged by size:
1842 1455 1239 726 627 426 325 217
137
BanII G_rGCy'C
Cuts at: 1391 3073 4313 1391
Size: 1682 1240 4072
Fragments arranged by size:
4072 1682 1240
BbeI G_GCGC'C
Cuts at: 4182 4182
Size: 6994
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 4023 928
Size: 971 192 1932 3899
Fragments arranged by size:
3899 1932 971 192
BbvCI CC'TCA_GC
Cuts at: 2033 2033
Size: 6994
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5185 5219 1903
Size: 34 3248 34 3678
Fragments arranged by size:
3678 3248 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4886 6096 4886
Size: 1210 5784
BclI T'GATC_A
Cuts at: 1548 2251 1548
Size: 703 6291
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2018 5605
Size: 107 122 71 631 605 337 9 3587
Cuts at: 5605 136
Size: 1525
Fragments arranged by size:
3587 1525 631 605 337 122 107 71
9
BglII A'GATC_T
Cuts at: 2769 3808 6989 2769
Size: 1039 3181 2774
Fragments arranged by size:
3181 2774 1039
BlpI GC'TnA_GC
Cuts at: 4411 4411
Size: 6994
Bme1580I G_kGCm'C
Cuts at: 1790 5055 6211 1790
Size: 3265 1156 2573
Fragments arranged by size:
3265 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6994
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4725 5683
Size: 416 53 83 399 24 1886 1422 958
Cuts at: 5683 442
Size: 1753
Fragments arranged by size:
1886 1753 1422 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4256 1056
Size: 3200 3794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2682 3551 3674 5654 1239
Size: 1443 869 123 1980 2579
Fragments arranged by size:
2579 1980 1443 869 123
Bpu10I CC'TnA_GC
Cuts at: 2033 2033
Size: 6994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 3578 4187 5119 5984 6282 2474
Size: 1104 609 932 865 298 3186
Fragments arranged by size:
3186 1104 932 865 609 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3596 5657 882
Size: 617 2097 2061 2219
Fragments arranged by size:
2219 2097 2061 617
BsaAI yAC'GTr
Cuts at: 493 1204 4785 493
Size: 711 3581 2702
Fragments arranged by size:
3581 2702 711
BsaBI GATnn'nnATC
Cuts at: 2027 6988 2027
Size: 4961 2033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4179 4801 5183 5818
Size: 53 83 186 936 2646 622 382 635
Cuts at: 5818 275
Size: 1451
Fragments arranged by size:
2646 1451 936 635 622 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5427 5825 6099 6246 6657 5427
Size: 398 274 147 411 5764
Fragments arranged by size:
5764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2979 3009 1178
Size: 30 165 30 1576 30 5163
Fragments arranged by size:
5163 1576 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2460 1186
Size: 1274 5720
BseYI C'CCAG_C
Cuts at: 2530 6197 6693 2530
Size: 3667 496 2831
Fragments arranged by size:
3667 2831 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2649 2886 3129 1163
Size: 573 913 237 243 5028
Fragments arranged by size:
5028 913 573 243 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3546 4444 5205 6233 1540
Size: 275 186 1545 898 761 1028 2301
Fragments arranged by size:
2301 1545 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3073 5055 5140 6211 3073
Size: 1982 85 1071 3856
Fragments arranged by size:
3856 1982 1071 85
BsmI GAATG_Cn'
Cuts at: 2622 4505 4598 2622
Size: 1883 93 5018
Fragments arranged by size:
5018 1883 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3073 4313 5055 5140 6211 1391
Size: 399 1283 1240 742 85 1071 2174
Fragments arranged by size:
2174 1283 1240 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6657 6657
Size: 6994
BspHI T'CATG_A
Cuts at: 4883 6613 4883
Size: 1730 5264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 4102 4349 844
Size: 1450 1808 247 3489
Fragments arranged by size:
3489 1808 1450 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2064 3832 4881 1394
Size: 429 241 1768 1049 3507
Fragments arranged by size:
3507 1768 1049 429 241
BsrDI GCAATG_nn'
Cuts at: 65 1248 5489 5663 65
Size: 1183 4241 174 1396
Fragments arranged by size:
4241 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2957 4197 5638 5825 1700
Size: 110 1147 1240 1441 187 2869
Fragments arranged by size:
2869 1441 1240 1147 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6994
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6994
BssSI C'ACGA_G
Cuts at: 5054 6066 5054
Size: 1012 5982
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3376 794
Size: 2582 4412
BstBI TT'CG_AA
Cuts at: 2161 5798 2161
Size: 3637 3357
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6994
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 3766 2578
Size: 1188 5806
BstYI r'GATC_y
Cuts at: 1972 2055 2432 2769 3808 4080 4168 4324 4691
Size: 83 377 337 1039 272 88 156 367
Cuts at: 4691 5076 5093 6545 6631 6643 6989 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1039 385 377 367 346 337
272 156 88 86 83 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3034 3144 3510 513
Size: 552 939 1030 110 366 3997
Fragments arranged by size:
3997 1030 939 552 366 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3115 487
Size: 683 56 1889 4366
Fragments arranged by size:
4366 1889 683 56
BtsI GCAGTG_nn'
Cuts at: 3186 4510 5304 5324 3186
Size: 1324 794 20 4856
Fragments arranged by size:
4856 1324 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3718 3753 529
Size: 35 3154 35 3770
Fragments arranged by size:
3770 3154 35 35
DraI TTT'AAA
Cuts at: 4298 4298
Size: 6994
DraIII CAC_nnn'GTG
Cuts at: 3376 3376
Size: 6994
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6001 809
Size: 507 4685 1802
Fragments arranged by size:
4685 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4086 4441 5330
Size: 54 1425 50 275 18 2256 355 889
Cuts at: 5330 6959 8
Size: 1629 43
Fragments arranged by size:
2256 1629 1425 889 355 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3929 4924 1353
Size: 2576 995 3423
Fragments arranged by size:
3423 2576 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3818 4019 5581 5955 6101 387
Size: 1064 138 2229 201 1562 374 146 1280
Fragments arranged by size:
2229 1562 1280 1064 374 201 146 138
EcoICRI GAG'CTC
Cuts at: 3071 3071
Size: 6994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2541 2682 3265 3551 3558 3674
Size: 393 403 506 141 583 286 7 116
Cuts at: 3674 3693 4001 4020 5057 5654 6441 6709 1239
Size: 19 308 19 1037 597 787 268 1524
Fragments arranged by size:
1524 1037 787 597 583 506 403 393
308 286 268 141 116 19 19 7
EcoNI CCTnn'n_nnAGG
Cuts at: 4073 4073
Size: 6994
EcoO109I rG'GnC_Cy
Cuts at: 2535 3043 3355 4151 2535
Size: 508 312 796 5378
Fragments arranged by size:
5378 796 508 312
EcoRI G'AATT_C
Cuts at: 3949 4303 4681 3949
Size: 354 378 6262
Fragments arranged by size:
6262 378 354
EcoRV GAT'ATC
Cuts at: 2314 2314
Size: 6994
FspI TGC'GCA
Cuts at: 5500 5500
Size: 6994
HaeII r_GCGC'y
Cuts at: 1678 4182 6141 1678
Size: 2504 1959 2531
Fragments arranged by size:
2531 2504 1959
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3114 3146 3391 3423 5635
Size: 32 135 32 1542 32 245 32 2212
Cuts at: 5635 5667 5709 5741 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2212 1542 245 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2659 3703 4338 4584 669
Size: 988 281 721 1044 635 246 3079
Fragments arranged by size:
3079 1044 988 721 635 281 246
HindIII A'AGCT_T
Cuts at: 748 3733 748
Size: 2985 4009
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KasI G'GCGC_C
Cuts at: 4178 4178
Size: 6994
KpnI G_GTAC'C
Cuts at: 2517 4319 2517
Size: 1802 5192
MfeI C'AATT_G
Cuts at: 4593 4593
Size: 6994
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6108 6292 1305
Size: 4803 184 2007
Fragments arranged by size:
4803 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4088 10
Size: 54 1425 343 2256 2916
Fragments arranged by size:
2916 2256 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3764 4952 5311 5470 518
Size: 648 2448 150 1188 359 159 2042
Fragments arranged by size:
2448 2042 1188 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 3526 3578 4249 4410 5087 6235 6480 1606
Size: 1920 52 671 161 677 1148 245 2120
Fragments arranged by size:
2120 1920 1148 677 671 245 161 52
NarI GG'CG_CC
Cuts at: 4179 4179
Size: 6994
NcoI C'CATG_G
Cuts at: 513 1065 2004 3034 513
Size: 552 939 1030 4473
Fragments arranged by size:
4473 1030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6994
NheI G'CTAG_C
Cuts at: 1052 4252 1052
Size: 3200 3794
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NspI r_CATG'y
Cuts at: 1132 1229 2323 3496 3800 4352 5897 1132
Size: 97 1094 1173 304 552 1545 2229
Fragments arranged by size:
2229 1545 1173 1094 552 304 97
PasI CC'CwG_GG
Cuts at: 1348 2677 1348
Size: 1329 5665
PciI A'CATG_T
Cuts at: 2319 3796 5893 2319
Size: 1477 2097 3420
Fragments arranged by size:
3420 2097 1477
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2668 1108
Size: 1560 5434
PfoI T'CCnGG_A
Cuts at: 3690 3690
Size: 6994
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 6576 6608 5063
Size: 32 1481 32 5449
Fragments arranged by size:
5449 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3043 3355 3043
Size: 312 6682
PshAI GACnn'nnGTC
Cuts at: 1613 3360 1613
Size: 1747 5247
PsiI TTA'TAA
Cuts at: 4564 4564
Size: 6994
PstI C_TGCA'G
Cuts at: 830 4099 4346 830
Size: 3269 247 3478
Fragments arranged by size:
3478 3269 247
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 3526 3578 4249 3526
Size: 52 671 6271
Fragments arranged by size:
6271 671 52
SacI G_AGCT'C
Cuts at: 3073 3073
Size: 6994
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 5242 6954 5242
Size: 1712 5282
SexAI A'CCwGG_T
Cuts at: 3647 4290 3647
Size: 643 6351
SfcI C'TryA_G
Cuts at: 826 1045 3579 4095 4342 5477 6158 6349 826
Size: 219 2534 516 247 1135 681 191 1471
Fragments arranged by size:
2534 1471 1135 681 516 247 219 191
SfoI GGC'GCC
Cuts at: 4180 4180
Size: 6994
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 4321 1872
Size: 2449 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 3365 3593 4202 5098 5999
Size: 197 926 510 912 228 609 896 901
Cuts at: 5999 6261 820
Size: 262 1553
Fragments arranged by size:
1553 926 912 901 896 609 510 262
228 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6994
SphI G_CATG'C
Cuts at: 1132 3496 4352 1132
Size: 2364 856 3774
Fragments arranged by size:
3774 2364 856
SspI AAT'ATT
Cuts at: 5 52 4918 5
Size: 47 4866 2081
Fragments arranged by size:
4866 2081 47
StuI AGG'CCT
Cuts at: 1517 2892 3017 1517
Size: 1375 125 5494
Fragments arranged by size:
5494 1375 125
StyI C'CwwG_G
Cuts at: 513 1065 2004 3034 3310 3876 3886 4433 513
Size: 552 939 1030 276 566 10 547 3074
Fragments arranged by size:
3074 1030 939 566 552 547 276 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2570 3213 3560 3740 5021 5038 5191
Size: 1255 990 643 347 180 1281 17 153
Cuts at: 5191 5376 5821 325
Size: 185 445 1498
Fragments arranged by size:
1498 1281 1255 990 643 445 347 185
180 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5240 6952 96
Size: 274 80 33 51 1443 3263 1712 138
Fragments arranged by size:
3263 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6954 6986 6954
Size: 32 6962
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6994
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2129 2129
Size: 6994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 4319 1870
Size: 2449 4545
XmnI GAAnn'nnTTC
Cuts at: 5123 5123
Size: 6994
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4802 276
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SrfI SwaI