Restriction map for
pFN21AE4568
(Circular) (Six-base) MAPSORT of: pFN21AE4568.seq Check: 4361 from: 1 to: 6868
pFN21AE4568 6868 bp
With 182 enzymes: SgfI *
March 28, 2012 14:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2943 4678 278
Size: 53 83 186 936 1407 1735 2468
Fragments arranged by size:
2468 1735 1407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2958 4211 1937
Size: 1021 1253 4594
Fragments arranged by size:
4594 1253 1021
Acc65I G'GTAC_C
Cuts at: 4189 4189
Size: 6868
AclI AA'CG_TT
Cuts at: 1563 4995 5368 1563
Size: 3432 373 3063
Fragments arranged by size:
3432 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 4179 4931 6315 6583 1632
Size: 713 1834 752 1384 268 1917
Fragments arranged by size:
1917 1834 1384 752 713 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6671
AflIII A'CryG_T
Cuts at: 1203 2916 5767 6744 1203
Size: 1713 2851 977 1327
Fragments arranged by size:
2851 1713 1327 977
AgeI A'CCGG_T
Cuts at: 5699 5699
Size: 6868
AhdI GACnn_n'nnGTC
Cuts at: 2661 5597 2661
Size: 2936 3932
AleI CACnn'nnGTG
Cuts at: 1166 3159 1166
Size: 1993 4875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6868
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2706 2738 2706
Size: 32 6836
AlwNI CAG_nnn'CTG
Cuts at: 2757 3238 3544 3790 4611 6183 2757
Size: 481 306 246 821 1572 3442
Fragments arranged by size:
3442 1572 821 481 306 246
ApaI G_GGCC'C
Cuts at: 3320 3320
Size: 6868
ApaLI G'TGCA_C
Cuts at: 4925 6081 4925
Size: 1156 5712
ApoI r'AATT_y
Cuts at: 1493 2246 4177 4403 4555 6664 1493
Size: 753 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 753 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2228 5422 160
Size: 872 1196 3194 1606
Fragments arranged by size:
3194 1606 1196 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2808 4193 1870
Size: 73 253 612 1385 4545
Fragments arranged by size:
4545 1385 612 253 73
AvrII C'CTAG_G
Cuts at: 2851 2851
Size: 6868
BamHI G'GATC_C
Cuts at: 4198 4565 4198
Size: 367 6501
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2337 2894 4189 5644
Size: 325 217 627 236 314 557 1295 1455
Cuts at: 5644 618
Size: 1842
Fragments arranged by size:
1842 1455 1295 627 557 325 314 236
217
BanII G_rGCy'C
Cuts at: 1391 2385 2497 3095 3320 3344 3893 4187 1391
Size: 994 112 598 225 24 549 294 4072
Fragments arranged by size:
4072 994 598 549 294 225 112 24
BbeI G_GCGC'C
Cuts at: 2027 2027
Size: 6868
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2604 2660 928
Size: 971 705 56 5136
Fragments arranged by size:
5136 971 705 56
BbvCI CC'TCA_GC
Cuts at: 3255 4035 3255
Size: 780 6088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3185 3219 5059 5093 1903
Size: 34 1248 34 1840 34 3678
Fragments arranged by size:
3678 1840 1248 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4760 5970 4760
Size: 1210 5658
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6868
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2105 2222
Size: 107 122 71 631 605 337 96 117
Cuts at: 2222 3630 5479 136
Size: 1408 1849 1525
Fragments arranged by size:
1849 1525 1408 631 605 337 122 117
107 96 71
BglII A'GATC_T
Cuts at: 6863 6863
Size: 6868
BlpI GC'TnA_GC
Cuts at: 4285 4285
Size: 6868
Bme1580I G_kGCm'C
Cuts at: 1790 2899 3320 3862 4929 6085 1790
Size: 1109 421 542 1067 1156 2573
Fragments arranged by size:
2573 1156 1109 1067 542 421
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6868
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2405 4599 5557
Size: 416 53 83 399 24 988 2194 958
Cuts at: 5557 442
Size: 1753
Fragments arranged by size:
2194 1753 988 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6868
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2423 3027 2423
Size: 604 6264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3237 3348 3827 3907 4043 5528 1239
Size: 1998 111 479 80 136 1485 2579
Fragments arranged by size:
2579 1998 1485 479 136 111 80
Bpu10I CC'TnA_GC
Cuts at: 2883 3255 4035 2883
Size: 372 780 5716
Fragments arranged by size:
5716 780 372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 3539 3731 3829 4993 5858 6156 2519
Size: 1020 192 98 1164 865 298 3231
Fragments arranged by size:
3231 1164 1020 865 298 192 98
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 2834 5531 882
Size: 617 1170 165 2697 2219
Fragments arranged by size:
2697 2219 1170 617 165
BsaAI yAC'GTr
Cuts at: 493 1204 2875 3799 4659 493
Size: 711 1671 924 860 2702
Fragments arranged by size:
2702 1671 924 860 711
BsaBI GATnn'nnATC
Cuts at: 6862 6862
Size: 6868
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 2940 4675 5057
Size: 53 83 186 936 491 916 1735 382
Cuts at: 5057 5692 275
Size: 635 1451
Fragments arranged by size:
1735 1451 936 916 635 491 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5301 5699 5973 6120 6531 5301
Size: 398 274 147 411 5638
Fragments arranged by size:
5638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2706 2736 1178
Size: 30 165 30 1303 30 5310
Fragments arranged by size:
5310 1303 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2754 2858 3539 3626 3716 4163 1186
Size: 940 628 104 681 87 90 447 3891
Fragments arranged by size:
3891 940 681 628 447 104 90 87
BseYI C'CCAG_C
Cuts at: 2573 6071 6567 2573
Size: 3498 496 2874
Fragments arranged by size:
3498 2874 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2992 1163
Size: 573 1256 5039
Fragments arranged by size:
5039 1256 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2164 4318 5079 6107 1540
Size: 275 186 163 2154 761 1028 2301
Fragments arranged by size:
2301 2154 1028 761 275 186 163
BsiHKAI G_wGCw'C
Cuts at: 2385 3087 3223 4929 5014 6085 2385
Size: 702 136 1706 85 1071 3168
Fragments arranged by size:
3168 1706 1071 702 136 85
BsmI GAATG_Cn'
Cuts at: 4379 4472 4379
Size: 93 6775
BsmBI CGTCTCn'nnnn_
Cuts at: 2509 2509
Size: 6868
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2497 2899 3087 3095 3223 3320
Size: 399 595 112 402 188 8 128 97
Cuts at: 3320 3344 3862 3893 4187 4929 5014 6085 1391
Size: 24 518 31 294 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 518 402 399 294
188 128 112 97 85 31 24 8
BspEI T'CCGG_A
Cuts at: 6531 6531
Size: 6868
BspHI T'CATG_A
Cuts at: 4757 6487 4757
Size: 1730 5138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3775 4223 844
Size: 2931 448 3489
Fragments arranged by size:
3489 2931 448
BsrBI CCG'CTC
Cuts at: 1394 1823 4755 1394
Size: 429 2932 3507
Fragments arranged by size:
3507 2932 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5363 5537 65
Size: 1183 4115 174 1396
Fragments arranged by size:
4115 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5512 5699 1700
Size: 110 3702 187 2869
Fragments arranged by size:
3702 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2266 96
Size: 2170 4698
BssHII G'CGCG_C
Cuts at: 1925 3916 1925
Size: 1991 4877
BssSI C'ACGA_G
Cuts at: 4928 5940 4928
Size: 1012 5856
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3117 794
Size: 2323 4545
BstBI TT'CG_AA
Cuts at: 2365 5672 2365
Size: 3307 3561
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6868
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2431
Size: 6868
BstYI r'GATC_y
Cuts at: 1972 2847 2862 3225 4026 4198 4565 4950 4967
Size: 875 15 363 801 172 367 385 17
Cuts at: 4967 6419 6505 6517 6863 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 875 801 385 367 363 346
172 86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2766 3042 3336 2766
Size: 276 294 6298
Fragments arranged by size:
6298 294 276
BtgI C'CryG_G
Cuts at: 513 1065 2004 3058 3990 513
Size: 552 939 1054 932 3391
Fragments arranged by size:
3391 1054 939 932 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6129
Fragments arranged by size:
6129 683 56
BtsI GCAGTG_nn'
Cuts at: 4384 5178 5198 4384
Size: 794 20 6054
Fragments arranged by size:
6054 794 20
ClaI AT'CG_AT
Cuts at: 1554 2389 1554
Size: 835 6033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6833
DraI TTT'AAA
Cuts at: 4172 4172
Size: 6868
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3725 5875 809
Size: 507 2409 2150 1802
Fragments arranged by size:
2409 2150 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2214 3993
Size: 54 1425 50 275 18 318 66 1779
Cuts at: 3993 4315 5204 6833 8
Size: 322 889 1629 43
Fragments arranged by size:
1779 1629 1425 889 322 318 275 66
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4315 1537
Size: 275 2503 4090
Fragments arranged by size:
4090 2503 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3516 3567 4140 4798 1353
Size: 2163 51 573 658 3423
Fragments arranged by size:
3423 2163 658 573 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 2936 5455 5829 5975 387
Size: 1064 138 534 813 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 813 534 374 146 138
EcoICRI GAG'CTC
Cuts at: 2383 2383
Size: 6868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 3237 3348 3827 3907 4043 4179
Size: 393 713 892 111 479 80 136 136
Cuts at: 4179 4931 5528 6315 6583 1239
Size: 752 597 787 268 1524
Fragments arranged by size:
1524 892 787 752 713 597 479 393
268 136 136 111 80
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 3194 4034 2696
Size: 498 840 5530
Fragments arranged by size:
5530 840 498
EcoO109I rG'GnC_Cy
Cuts at: 2200 3038 2200
Size: 838 6030
EcoRI G'AATT_C
Cuts at: 2246 4177 4555 2246
Size: 1931 378 4559
Fragments arranged by size:
4559 1931 378
FspI TGC'GCA
Cuts at: 4097 5374 4097
Size: 1277 5591
HaeII r_GCGC'y
Cuts at: 1678 2027 6015 1678
Size: 349 3988 2531
Fragments arranged by size:
3988 2531 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 2647 2679 3210
Size: 32 135 32 884 32 159 32 531
Cuts at: 3210 3242 3543 3575 4139 4171 5509 5541 5583
Size: 32 301 32 564 32 1338 32 42
Cuts at: 5583 5615 1373
Size: 32 2626
Fragments arranged by size:
2626 1338 884 564 531 301 159 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4212 4458 669
Size: 988 281 2274 246 3079
Fragments arranged by size:
3079 2274 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6868
HpaI GTT'AAC
Cuts at: 4458 4458
Size: 6868
KasI G'GCGC_C
Cuts at: 2023 2023
Size: 6868
KpnI G_GTAC'C
Cuts at: 4193 4193
Size: 6868
MfeI C'AATT_G
Cuts at: 3115 4467 3115
Size: 1352 5516
MluI A'CGCG_T
Cuts at: 6744 6744
Size: 6868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2931 5982 6166 1305
Size: 1626 3051 184 2007
Fragments arranged by size:
3051 2007 1626 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 3995 10
Size: 54 1425 343 384 1779 2883
Fragments arranged by size:
2883 1779 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3159 3512 3656 4826 5185 5344 518
Size: 648 1993 353 144 1170 359 159 2042
Fragments arranged by size:
2042 1993 1170 648 359 353 159 144
MspA1I CmG'CkG
Cuts at: 1606 2064 2085 2927 3691 4021 4111 4284 4961
Size: 458 21 842 764 330 90 173 677
Cuts at: 4961 6109 6354 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 842 764 677 458 330 245
173 90 21
NarI GG'CG_CC
Cuts at: 2024 2024
Size: 6868
NcoI C'CATG_G
Cuts at: 513 1065 2004 3058 3990 513
Size: 552 939 1054 932 3391
Fragments arranged by size:
3391 1054 939 932 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6868
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6868
NotI GC'GGCC_GC
Cuts at: 4315 4315
Size: 6868
NspI r_CATG'y
Cuts at: 1132 1229 2872 2920 4226 5771 1132
Size: 97 1643 48 1306 1545 2229
Fragments arranged by size:
2229 1643 1545 1306 97 48
PasI CC'CwG_GG
Cuts at: 1348 2659 1348
Size: 1311 5557
PciI A'CATG_T
Cuts at: 2916 5767 2916
Size: 2851 4017
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6868
PfoI T'CCnGG_A
Cuts at: 3729 3804 3729
Size: 75 6793
PmeI GTTT'AAAC
Cuts at: 4172 4172
Size: 6868
PmlI CAC'GTG
Cuts at: 3799 3799
Size: 6868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2706 2738 4937 4969 6450 6482 2706
Size: 32 2199 32 1481 32 3092
Fragments arranged by size:
3092 2199 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2200
Size: 6868
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6868
PsiI TTA'TAA
Cuts at: 4438 4438
Size: 6868
PspOMI G'GGCC_C
Cuts at: 3316 3316
Size: 6868
PstI C_TGCA'G
Cuts at: 830 3505 3537 4116 4220 830
Size: 2675 32 579 104 3478
Fragments arranged by size:
3478 2675 579 104 32
PvuI CG_AT'CG
Cuts at: 2001 2164 2001
Size: 163 6705
PvuII CAG'CTG
Cuts at: 2927 4021 4111 2927
Size: 1094 90 5684
Fragments arranged by size:
5684 1094 90
SacI G_AGCT'C
Cuts at: 2385 2385
Size: 6868
SalI G'TCGA_C
Cuts at: 1936 4210 1936
Size: 2274 4594
SbfI CC_TGCA'GG
Cuts at: 4220 4220
Size: 6868
ScaI AGT'ACT
Cuts at: 5116 6828 5116
Size: 1712 5156
SfcI C'TryA_G
Cuts at: 826 1045 2016 3501 3533 3686 4112 4216 5351
Size: 219 971 1485 32 153 426 104 1135
Cuts at: 5351 6032 6223 826
Size: 681 191 1471
Fragments arranged by size:
1485 1471 1135 971 681 426 219 191
153 104 32
SfoI GGC'GCC
Cuts at: 2025 2025
Size: 6868
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6868
SmaI CCC'GGG
Cuts at: 1872 4195 1872
Size: 2323 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 2808 3554 3746 3844 4972
Size: 197 926 555 310 746 192 98 1128
Cuts at: 4972 5873 6135 820
Size: 901 262 1553
Fragments arranged by size:
1553 1128 926 901 746 555 310 262
197 192 98
SnaBI TAC'GTA
Cuts at: 493 4659 493
Size: 4166 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6868
SphI G_CATG'C
Cuts at: 1132 4226 1132
Size: 3094 3774
SspI AAT'ATT
Cuts at: 5 52 4792 5
Size: 47 4740 2081
Fragments arranged by size:
4740 2081 47
StuI AGG'CCT
Cuts at: 1517 3884 1517
Size: 2367 4501
StyI C'CwwG_G
Cuts at: 513 1065 2004 2184 2424 2851 3058 3990 3996
Size: 552 939 180 240 427 207 932 6
Cuts at: 3996 4307 513
Size: 311 3074
Fragments arranged by size:
3074 939 932 552 427 311 240 207
180 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2595 3014 4895 4912 5065 5250 5695
Size: 1255 1015 419 1881 17 153 185 445
Cuts at: 5695 325
Size: 1498
Fragments arranged by size:
1881 1498 1255 1015 445 419 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2266 2919 5114
Size: 274 80 33 51 1443 289 653 2195
Cuts at: 5114 6826 96
Size: 1712 138
Fragments arranged by size:
2195 1712 1443 653 289 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3276 3308 6828 6860 3276
Size: 32 3520 32 3284
Fragments arranged by size:
3520 3284 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6868
XbaI T'CTAG_A
Cuts at: 4204 4204
Size: 6868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4003 4003
Size: 6868
XhoI C'TCGA_G
Cuts at: 1943 2808 1943
Size: 865 6003
XmaI C'CCGG_G
Cuts at: 1870 4193 1870
Size: 2323 4545
XmnI GAAnn'nnTTC
Cuts at: 4044 4997 4044
Size: 953 5915
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2941 4676 276
Size: 53 83 186 936 1407 1735 2468
Fragments arranged by size:
2468 1735 1407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI