Restriction map for
pFN21AE4559
(Circular) (Six-base) MAPSORT of: pFN21AE4559.seq Check: 1191 from: 1 to: 6757
pFN21AE4559 6757 bp
With 182 enzymes: SgfI *
March 28, 2012 14:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4567 278
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4100 1937
Size: 2163 4594
Acc65I G'GTAC_C
Cuts at: 4078 4078
Size: 6757
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 2523 3867 4820 6204 6472 1632
Size: 872 19 1344 953 1384 268 1917
Fragments arranged by size:
1917 1384 1344 953 872 268 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6560
AflIII A'CryG_T
Cuts at: 1203 5656 6633 1203
Size: 4453 977 1327
Fragments arranged by size:
4453 1327 977
AgeI A'CCGG_T
Cuts at: 5588 5588
Size: 6757
AhdI GACnn_n'nnGTC
Cuts at: 5486 5486
Size: 6757
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6757
AlwNI CAG_nnn'CTG
Cuts at: 4500 6072 4500
Size: 1572 5185
ApaI G_GGCC'C
Cuts at: 2184 2203 2559 2658 2693 2730 2819 2999 3172
Size: 19 356 99 35 37 89 180 173
Cuts at: 3172 3431 3486 3696 3786 3864 3970 2184
Size: 259 55 210 90 78 106 4971
Fragments arranged by size:
4971 356 259 210 180 173 106 99
90 89 78 55 37 35 19
ApaLI G'TGCA_C
Cuts at: 4814 5970 4814
Size: 1156 5601
ApoI r'AATT_y
Cuts at: 1493 4066 4292 4444 6553 1493
Size: 2573 226 152 2109 1697
Fragments arranged by size:
2573 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5311 160
Size: 872 4279 1606
Fragments arranged by size:
4279 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2123 2185 2329 2339 2425 2668
Size: 73 108 72 62 144 10 86 243
Cuts at: 2668 2694 2723 3479 3514 3653 3732 3779 4082
Size: 26 29 756 35 139 79 47 303
Cuts at: 4082 1870
Size: 4545
Fragments arranged by size:
4545 756 303 243 144 139 108 86
79 73 72 62 47 35 29 26
10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3739 3772 3739
Size: 33 6724
BamHI G'GATC_C
Cuts at: 2772 4087 4454 2772
Size: 1315 367 5075
Fragments arranged by size:
5075 1315 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 3302 3437 3455 3649
Size: 325 217 627 510 1005 135 18 194
Cuts at: 3649 3887 3956 4078 5533 618
Size: 238 69 122 1455 1842
Fragments arranged by size:
1842 1455 1005 627 510 325 238 217
194 135 122 69 18
BanII G_rGCy'C
Cuts at: 1391 2184 2203 2559 2658 2693 2730 2819 2999
Size: 793 19 356 99 35 37 89 180
Cuts at: 2999 3161 3172 3199 3431 3486 3696 3786 3864
Size: 162 11 27 232 55 210 90 78
Cuts at: 3864 3970 4076 1391
Size: 106 106 4072
Fragments arranged by size:
4072 793 356 232 210 180 162 106
106 99 90 89 78 55 37 35
27 19 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3341 928
Size: 971 1442 4344
Fragments arranged by size:
4344 1442 971
BbvCI CC'TCA_GC
Cuts at: 3997 3997
Size: 6757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3596 3630 4948 4982 1903
Size: 34 1659 34 1318 34 3678
Fragments arranged by size:
3678 1659 1318 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4649 5859 3740
Size: 909 1210 4638
Fragments arranged by size:
4638 1210 909
BclI T'GATC_A
Cuts at: 1548 3600 1548
Size: 2052 4705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2106 2142
Size: 107 122 71 631 605 403 31 36
Cuts at: 2142 2341 2418 2670 2725 2931 3156 3183 3228
Size: 199 77 252 55 206 225 27 45
Cuts at: 3228 3255 3264 3435 3507 3965 5368 136
Size: 27 9 171 72 458 1403 1525
Fragments arranged by size:
1525 1403 631 605 458 403 252 225
206 199 171 122 107 77 72 71
55 45 36 31 27 27 9
BglII A'GATC_T
Cuts at: 6752 6752
Size: 6757
BlpI GC'TnA_GC
Cuts at: 3160 4174 3160
Size: 1014 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2203 2302 2528 2559 2658 2693 2730
Size: 394 19 99 226 31 99 35 37
Cuts at: 2730 2819 2999 3172 3431 3486 3696 3786 3864
Size: 89 180 173 259 55 210 90 78
Cuts at: 3864 3885 3970 4818 5974 1790
Size: 21 85 848 1156 2573
Fragments arranged by size:
2573 1156 848 394 259 226 210 180
173 99 99 90 89 85 78 55
37 35 31 21 19
BmgBI CAC'GTC
Cuts at: 1318 3280 1318
Size: 1962 4795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3380 3771 4488
Size: 416 53 83 399 24 1963 391 717
Cuts at: 4488 5446 442
Size: 958 1753
Fragments arranged by size:
1963 1753 958 717 416 399 391 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6757
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 6757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 3114 3132 5417 1239
Size: 912 963 18 2285 2579
Fragments arranged by size:
2579 2285 963 912 18
Bpu10I CC'TnA_GC
Cuts at: 3997 3997
Size: 6757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4882 5747 6045 4882
Size: 865 298 5594
Fragments arranged by size:
5594 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3234 3315 3768 3846 5420 882
Size: 617 1735 81 453 78 1574 2219
Fragments arranged by size:
2219 1735 1574 617 453 81 78
BsaAI yAC'GTr
Cuts at: 493 1204 4548 493
Size: 711 3344 2702
Fragments arranged by size:
3344 2702 711
BsaBI GATnn'nnATC
Cuts at: 6751 6751
Size: 6757
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3105 3510 4564 4946
Size: 53 83 186 936 1572 405 1054 382
Cuts at: 4946 5581 275
Size: 635 1451
Fragments arranged by size:
1572 1451 1054 936 635 405 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5190 5588 5862 6009 6420 5190
Size: 398 274 147 411 5527
Fragments arranged by size:
5527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6532
Fragments arranged by size:
6532 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3822 1186
Size: 2636 4121
BseYI C'CCAG_C
Cuts at: 2231 2344 3065 3073 3377 3424 3769 5960 6456
Size: 113 721 8 304 47 345 2191 496
Cuts at: 6456 2231
Size: 2532
Fragments arranged by size:
2532 2191 721 496 345 304 113 47
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 3289 4207 4968 5996 1540
Size: 275 186 69 1219 918 761 1028 2301
Fragments arranged by size:
2301 1219 1028 918 761 275 186 69
BsiHKAI G_wGCw'C
Cuts at: 2983 3916 4818 4903 5974 2983
Size: 933 902 85 1071 3766
Fragments arranged by size:
3766 1071 933 902 85
BsmI GAATG_Cn'
Cuts at: 2571 4268 4361 2571
Size: 1697 93 4967
Fragments arranged by size:
4967 1697 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2203 2302 2528 2559 2658 2693
Size: 399 394 19 99 226 31 99 35
Cuts at: 2693 2730 2819 2983 2999 3161 3172 3199 3431
Size: 37 89 164 16 162 11 27 232
Cuts at: 3431 3486 3696 3786 3864 3885 3916 3970 4076
Size: 55 210 90 78 21 31 54 106
Cuts at: 4076 4818 4903 5974 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 232 226 210
164 162 106 99 99 90 89 85
78 55 54 37 35 31 31 27
21 19 16 11
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 4646 6376 4646
Size: 1730 5027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 4112 844
Size: 2482 786 3489
Fragments arranged by size:
3489 2482 786
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 2391 3029 4644 1394
Size: 429 209 359 638 1615 3507
Fragments arranged by size:
3507 1615 638 429 359 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 5252 5426 65
Size: 1183 4004 174 1396
Fragments arranged by size:
4004 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2660 3083 5401 5588 1700
Size: 110 850 423 2318 187 2869
Fragments arranged by size:
2869 2318 850 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6757
BssHII G'CGCG_C
Cuts at: 1925 2017 1925
Size: 92 6665
BssSI C'ACGA_G
Cuts at: 4817 5829 4817
Size: 1012 5745
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6757
BstBI TT'CG_AA
Cuts at: 5561 5561
Size: 6757
BstEII G'GTnAC_C
Cuts at: 1184 2636 2798 2843 3719 1184
Size: 1452 162 45 876 4222
Fragments arranged by size:
4222 1452 876 162 45
BstXI CCAn_nnnn'nTGG
Cuts at: 3385 3385
Size: 6757
BstYI r'GATC_y
Cuts at: 1972 2772 3940 3984 4087 4454 4839 4856 6308
Size: 800 1168 44 103 367 385 17 1452
Cuts at: 6308 6394 6406 6752 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1168 800 385 367 346 103
86 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 3315 3635 3315
Size: 320 6437
BtgI C'CryG_G
Cuts at: 513 1065 2004 2416 2830 3751 513
Size: 552 939 412 414 921 3519
Fragments arranged by size:
3519 939 921 552 414 412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6018
Fragments arranged by size:
6018 683 56
BtsI GCAGTG_nn'
Cuts at: 4273 5067 5087 4273
Size: 794 20 5943
Fragments arranged by size:
5943 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6722
DraI TTT'AAA
Cuts at: 4061 4061
Size: 6757
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5764 809
Size: 507 4448 1802
Fragments arranged by size:
4448 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2067 2134
Size: 54 1425 50 275 18 177 60 67
Cuts at: 2134 2883 2923 2950 3148 3901 4204 5093 6722
Size: 749 40 27 198 753 303 889 1629
Cuts at: 6722 8
Size: 43
Fragments arranged by size:
1629 1425 889 753 749 303 275 198
177 67 60 54 50 43 40 27
18
EagI C'GGCC_G
Cuts at: 1537 1812 2067 4204 1537
Size: 275 255 2137 4090
Fragments arranged by size:
4090 2137 275 255
EarI CTCTTCn'nnn_
Cuts at: 1353 4687 1353
Size: 3334 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5344 5718 5864 387
Size: 1064 138 3755 374 146 1280
Fragments arranged by size:
3755 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2504 2523 3114 3132 3867 4820
Size: 393 519 353 19 591 18 735 953
Cuts at: 4820 5417 6204 6472 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 953 787 735 597 591 519 393
353 268 19 18
EcoO109I rG'GnC_Cy
Cuts at: 2181 2199 2200 2324 2429 2465 2546 2555 2556
Size: 18 1 124 105 36 81 9 1
Cuts at: 2556 2582 2689 2690 2727 2744 2815 2995 2996
Size: 26 107 1 37 17 71 180 1
Cuts at: 2996 3168 3169 3185 3257 3265 3311 3374 3428
Size: 172 1 16 72 8 46 63 54
Cuts at: 3428 3446 3483 3693 3701 3737 3860 3861 3869
Size: 18 37 210 8 36 123 1 8
Cuts at: 3869 3966 2181
Size: 97 4972
Fragments arranged by size:
4972 210 180 172 124 123 107 105
97 81 72 71 63 54 46 37
37 36 36 26 18 18 17 16
9 8 8 8 1 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 4066 4444 4066
Size: 378 6379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3660 3660
Size: 6757
FseI GG_CCGG'CC
Cuts at: 3087 3087
Size: 6757
FspI TGC'GCA
Cuts at: 5263 5263
Size: 6757
HaeII r_GCGC'y
Cuts at: 1678 2511 2786 3732 5904 1678
Size: 833 275 946 2172 2531
Fragments arranged by size:
2531 2172 946 833 275
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5398 5430 5472 5504 1373
Size: 32 135 32 3826 32 42 32 2626
Fragments arranged by size:
3826 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4101 4347 669
Size: 988 281 2163 246 3079
Fragments arranged by size:
3079 2163 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6757
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KpnI G_GTAC'C
Cuts at: 4082 4082
Size: 6757
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3366 3370 5871 6055 1305
Size: 2061 4 2501 184 2007
Fragments arranged by size:
2501 2061 2007 184 4
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2952 3150 10
Size: 54 1425 343 1120 198 3617
Fragments arranged by size:
3617 1425 1120 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4715 5074 5233 518
Size: 648 3549 359 159 2042
Fragments arranged by size:
3549 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2418 2832 3164 3381 3462 3680 3753 4173
Size: 812 414 332 217 81 218 73 420
Cuts at: 4173 4850 5998 6243 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 812 677 420 414 332 245
218 217 81 73
NaeI GCC'GGC
Cuts at: 2662 3085 2662
Size: 423 6334
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5266
Fragments arranged by size:
5266 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6757
NgoMIV G'CCGG_C
Cuts at: 2660 3083 2660
Size: 423 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6757
NotI GC'GGCC_GC
Cuts at: 2067 4204 2067
Size: 2137 4620
NspI r_CATG'y
Cuts at: 1132 1229 2911 3127 4115 5660 1132
Size: 97 1682 216 988 1545 2229
Fragments arranged by size:
2229 1682 1545 988 216 97
PasI CC'CwG_GG
Cuts at: 1348 2114 2303 2552 2651 2732 3262 3442 3689
Size: 766 189 249 99 81 530 180 247
Cuts at: 3689 3970 1348
Size: 281 4135
Fragments arranged by size:
4135 766 530 281 249 247 189 180
99 81
PciI A'CATG_T
Cuts at: 5656 5656
Size: 6757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6757
PfoI T'CCnGG_A
Cuts at: 2470 2983 3190 3295 3934 2470
Size: 513 207 105 639 5293
Fragments arranged by size:
5293 639 513 207 105
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4826 4858 6339 6371 4826
Size: 32 1481 32 5212
Fragments arranged by size:
5212 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2324 2429 2465 2546 2582 2744 3374 3446 3701
Size: 105 36 81 36 162 630 72 255
Cuts at: 3701 3737 3869 2324
Size: 36 132 5212
Fragments arranged by size:
5212 630 255 162 132 105 81 72
36 36 36
PshAI GACnn'nnGTC
Cuts at: 1613 2742 3291 1613
Size: 1129 549 5079
Fragments arranged by size:
5079 1129 549
PsiI TTA'TAA
Cuts at: 4327 4327
Size: 6757
PspOMI G'GGCC_C
Cuts at: 2180 2199 2555 2654 2689 2726 2815 2995 3168
Size: 19 356 99 35 37 89 180 173
Cuts at: 3168 3427 3482 3692 3782 3860 3966 2180
Size: 259 55 210 90 78 106 4971
Fragments arranged by size:
4971 356 259 210 180 173 106 99
90 89 78 55 37 35 19
PspXI vC'TCGA_Gb
Cuts at: 3732 3732
Size: 6757
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3322 3354 3322
Size: 32 6725
PstI C_TGCA'G
Cuts at: 830 2688 3507 4109 830
Size: 1858 819 602 3478
Fragments arranged by size:
3478 1858 819 602
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6757
PvuII CAG'CTG
Cuts at: 3680 3680
Size: 6757
SacII CC_GC'GG
Cuts at: 2419 2833 3754 2419
Size: 414 921 5422
Fragments arranged by size:
5422 921 414
SalI G'TCGA_C
Cuts at: 1936 4099 1936
Size: 2163 4594
SanDI GG'GwC_CC
Cuts at: 2324 2582 2744 3374 3446 2324
Size: 258 162 630 72 5635
Fragments arranged by size:
5635 630 258 162 72
SbfI CC_TGCA'GG
Cuts at: 3507 4109 3507
Size: 602 6155
ScaI AGT'ACT
Cuts at: 5005 6717 5005
Size: 1712 5045
SexAI A'CCwGG_T
Cuts at: 2857 2857
Size: 6757
SfcI C'TryA_G
Cuts at: 826 1045 2684 3503 4105 5240 5921 6112 826
Size: 219 1639 819 602 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 819 681 602 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2106 2418 2725 3183 3255 3264 2106
Size: 312 307 458 72 9 5599
Fragments arranged by size:
5599 458 312 307 72 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6757
SmaI CCC'GGG
Cuts at: 1872 2125 2187 2331 2341 2427 2670 2696 3481
Size: 253 62 144 10 86 243 26 785
Cuts at: 3481 3516 3655 3781 4084 1872
Size: 35 139 126 303 4545
Fragments arranged by size:
4545 785 303 253 243 144 139 126
86 62 35 26 10
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3732 4861 5762 6024 820
Size: 197 926 1789 1129 901 262 1553
Fragments arranged by size:
1789 1553 1129 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4548 493
Size: 4055 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6757
SphI G_CATG'C
Cuts at: 1132 2911 3127 4115 1132
Size: 1779 216 988 3774
Fragments arranged by size:
3774 1779 988 216
SrfI GCCC'GGGC
Cuts at: 2341 2341
Size: 6757
SspI AAT'ATT
Cuts at: 5 52 4681 5
Size: 47 4629 2081
Fragments arranged by size:
4629 2081 47
StuI AGG'CCT
Cuts at: 1517 2358 1517
Size: 841 5916
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 2838 2865 3865 3874 4006
Size: 552 939 288 546 27 1000 9 132
Cuts at: 4006 4196 513
Size: 190 3074
Fragments arranged by size:
3074 1000 939 552 546 288 190 132
27 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 2817 3065 3455 4784 4801 4954
Size: 1255 922 315 248 390 1329 17 153
Cuts at: 4954 5139 5584 325
Size: 185 445 1498
Fragments arranged by size:
1498 1329 1255 922 445 390 315 248
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5003 6715 96
Size: 274 80 33 51 1443 3026 1712 138
Fragments arranged by size:
3026 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2782 2814 3430 3462 6717 6749 2782
Size: 32 616 32 3255 32 2790
Fragments arranged by size:
3255 2790 616 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2859 1316
Size: 1543 5214
XbaI T'CTAG_A
Cuts at: 4093 4093
Size: 6757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3766 3766
Size: 6757
XhoI C'TCGA_G
Cuts at: 1943 3732 1943
Size: 1789 4968
XmaI C'CCGG_G
Cuts at: 1870 2123 2185 2329 2339 2425 2668 2694 3479
Size: 253 62 144 10 86 243 26 785
Cuts at: 3479 3514 3653 3779 4082 1870
Size: 35 139 126 303 4545
Fragments arranged by size:
4545 785 303 253 243 144 139 126
86 62 35 26 10
XmnI GAAnn'nnTTC
Cuts at: 4886 4886
Size: 6757
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4565 276
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FspAI KasI
NarI NruI NsiI PacI PmlI RsrII SacI SapI
SfoI SwaI