Restriction map for
pFN21AE4556
(Circular) (Six-base) MAPSORT of: pFN21AE4556.seq Check: 9677 from: 1 to: 6736
pFN21AE4556 6736 bp
With 182 enzymes: SgfI *
March 28, 2012 14:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4079 1937
Size: 2142 4594
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 4799 6183 6451 1632
Size: 602 2565 1384 268 1917
Fragments arranged by size:
2565 1917 1384 602 268
AfeI AGC'GCT
Cuts at: 2621 2621
Size: 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 2922 3448 5635 6612 1203
Size: 1719 526 2187 977 1327
Fragments arranged by size:
2187 1719 1327 977 526
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 5182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6736
AlwNI CAG_nnn'CTG
Cuts at: 4479 6051 4479
Size: 1572 5164
ApaI G_GGCC'C
Cuts at: 2063 2376 2701 3195 3625 3794 2063
Size: 313 325 494 430 169 5005
Fragments arranged by size:
5005 494 430 325 313 169
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 2807 4045 4271 4423 6532 1493
Size: 1314 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1314 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3753 5290 160
Size: 872 2721 1537 1606
Fragments arranged by size:
2721 1606 1537 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 2369 3786 4061 1870
Size: 73 426 1417 275 4545
Fragments arranged by size:
4545 1417 426 275 73
BamHI G'GATC_C
Cuts at: 3351 3687 4066 4433 3351
Size: 336 379 367 5654
Fragments arranged by size:
5654 379 367 336
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3874 4057 5512 618
Size: 325 217 627 2087 183 1455 1842
Fragments arranged by size:
2087 1842 1455 627 325 217 183
BanII G_rGCy'C
Cuts at: 1391 2063 2277 2376 2701 2784 3195 3625 3794
Size: 672 214 99 325 83 411 430 169
Cuts at: 3794 4055 1391
Size: 261 4072
Fragments arranged by size:
4072 672 430 411 325 261 214 169
99 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5765
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2987 3021 4927 4961 1903
Size: 34 50 34 966 34 1906 34 3678
Fragments arranged by size:
3678 1906 966 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 2796 3384 1548
Size: 1248 588 4900
Fragments arranged by size:
4900 1248 588
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2903 5347 136
Size: 107 122 71 631 605 1231 2444 1525
Fragments arranged by size:
2444 1525 1231 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6731 6731
Size: 6736
BlpI GC'TnA_GC
Cuts at: 4153 4153
Size: 6736
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2187 2259 2376 2701 3195 3625 3794
Size: 273 124 72 117 325 494 430 169
Cuts at: 3794 3816 3877 4797 5953 1790
Size: 22 61 920 1156 2573
Fragments arranged by size:
2573 1156 920 494 430 325 273 169
124 117 72 61 22
BmgBI CAC'GTC
Cuts at: 1318 3449 1318
Size: 2131 4605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2842 3675 4467
Size: 416 53 83 399 24 1425 833 792
Cuts at: 4467 5425 442
Size: 958 1753
Fragments arranged by size:
1753 1425 958 833 792 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3432 3887 5396 1239
Size: 2193 455 1509 2579
Fragments arranged by size:
2579 2193 1509 455
Bpu10I CC'TnA_GC
Cuts at: 2762 2762
Size: 6736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 3258 3389 4861 5726 6024 2114
Size: 1144 131 1472 865 298 2826
Fragments arranged by size:
2826 1472 1144 865 298 131
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5399 882
Size: 617 3900 2219
Fragments arranged by size:
3900 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4527 493
Size: 711 3323 2702
Fragments arranged by size:
3323 2702 711
BsaBI GATnn'nnATC
Cuts at: 6730 6730
Size: 6736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4543 4925 5560 275
Size: 53 83 186 936 3010 382 635 1451
Fragments arranged by size:
3010 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3258 5169 5567 5841 5988 6399 3258
Size: 1911 398 274 147 411 3595
Fragments arranged by size:
3595 1911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2526 2556 1178
Size: 30 165 30 1123 30 5358
Fragments arranged by size:
5358 1123 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2440 2510 1186
Size: 1254 70 5412
Fragments arranged by size:
5412 1254 70
BseYI C'CCAG_C
Cuts at: 2228 2344 3023 3289 3582 3816 5939 6435 2228
Size: 116 679 266 293 234 2123 496 2529
Fragments arranged by size:
2529 2123 679 496 293 266 234 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2406 2573 1163
Size: 573 670 167 5326
Fragments arranged by size:
5326 670 573 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4186 4947 5975 1540
Size: 275 186 1327 858 761 1028 2301
Fragments arranged by size:
2301 1327 1028 858 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2674 4797 4882 5953 2674
Size: 2123 85 1071 3457
Fragments arranged by size:
3457 2123 1071 85
BsmI GAATG_Cn'
Cuts at: 2166 4247 4340 2166
Size: 2081 93 4562
Fragments arranged by size:
4562 2081 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2187 2259 2277 2376 2674 2701
Size: 399 273 124 72 18 99 298 27
Cuts at: 2701 2784 3195 3625 3794 3816 3877 4055 4797
Size: 83 411 430 169 22 61 178 742
Cuts at: 4797 4882 5953 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 430 411 399 298 273
178 169 124 99 85 83 72 61
27 22 18
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 2269 2338 4625 6355 2269
Size: 69 2287 1730 2650
Fragments arranged by size:
2650 2287 1730 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 2545 2610 4091 844
Size: 1575 126 65 1481 3489
Fragments arranged by size:
3489 1575 1481 126 65
BsrBI CCG'CTC
Cuts at: 1394 1823 2448 4623 1394
Size: 429 625 2175 3507
Fragments arranged by size:
3507 2175 625 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2086 5231 5405 65
Size: 1183 838 3145 174 1396
Fragments arranged by size:
3145 1396 1183 838 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 5380 5567 1700
Size: 110 395 3175 187 2869
Fragments arranged by size:
3175 2869 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 2919 96
Size: 2823 3913
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 4796 5808 4796
Size: 1012 5724
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3338 794
Size: 2544 4192
BstBI TT'CG_AA
Cuts at: 5540 5540
Size: 6736
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6736
BstXI CCAn_nnnn'nTGG
Cuts at: 3725 3725
Size: 6736
BstYI r'GATC_y
Cuts at: 1972 3351 3687 4066 4433 4818 4835 6287 6373
Size: 1379 336 379 367 385 17 1452 86
Cuts at: 6373 6385 6731 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1379 385 379 367 346 336
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2363 2363
Size: 6736
BtgI C'CryG_G
Cuts at: 513 1065 2718 2899 513
Size: 552 1653 181 4350
Fragments arranged by size:
4350 1653 552 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5997
Fragments arranged by size:
5997 683 56
BtsI GCAGTG_nn'
Cuts at: 2874 3567 3892 4252 5046 5066 2874
Size: 693 325 360 794 20 4544
Fragments arranged by size:
4544 794 693 360 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 4040 4040
Size: 6736
DraIII CAC_nnn'GTG
Cuts at: 3017 3017
Size: 6736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2279 3601
Size: 54 1425 50 275 18 307 142 1322
Cuts at: 3601 3769 3779 4183 5072 6701 8
Size: 168 10 404 889 1629 43
Fragments arranged by size:
1629 1425 1322 889 404 307 275 168
142 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 4183 1537
Size: 275 2371 4090
Fragments arranged by size:
4090 2371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3307 4666 1353
Size: 1954 1359 3423
Fragments arranged by size:
3423 1954 1359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5323 5697 5843 387
Size: 1064 138 3734 374 146 1280
Fragments arranged by size:
3734 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 3432 3887 4799 5396 6183 6451
Size: 393 602 1198 455 912 597 787 268
Cuts at: 6451 1239
Size: 1524
Fragments arranged by size:
1524 1198 912 787 602 597 455 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2060 2373 2697 2698 2707 2789 3028 3092 3790
Size: 313 324 1 9 82 239 64 698
Cuts at: 3790 3791 2060
Size: 1 5005
Fragments arranged by size:
5005 698 324 313 239 82 64 9
1 1
EcoRI G'AATT_C
Cuts at: 2807 4045 4423 2807
Size: 1238 378 5120
Fragments arranged by size:
5120 1238 378
FspI TGC'GCA
Cuts at: 2078 3566 5242 2078
Size: 1488 1676 3572
Fragments arranged by size:
3572 1676 1488
HaeII r_GCGC'y
Cuts at: 1678 2623 5883 1678
Size: 945 3260 2531
Fragments arranged by size:
3260 2531 945
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 2500 2532 2781
Size: 32 135 32 845 32 51 32 249
Cuts at: 2781 2813 3940 3947 3972 3979 5377 5409 5451
Size: 32 1127 7 25 7 1398 32 42
Cuts at: 5451 5483 1373
Size: 32 2626
Fragments arranged by size:
2626 1398 1127 845 249 135 51 42
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3801 4080 4326 669
Size: 988 281 1863 279 246 3079
Fragments arranged by size:
3079 1863 988 281 279 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6736
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 4335 4335
Size: 6736
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2478 3660 5850 6034 1305
Size: 1173 1182 2190 184 2007
Fragments arranged by size:
2190 2007 1182 1173 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2139 2281 3603 3781 10
Size: 54 1425 343 307 142 1322 178 2965
Fragments arranged by size:
2965 1425 1322 343 307 178 142 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 3609 3827 3854 4694 5053 5212
Size: 648 1554 889 218 27 840 359 159
Cuts at: 5212 518
Size: 2042
Fragments arranged by size:
2042 1554 889 840 648 359 218 159
27
MspA1I CmG'CkG
Cuts at: 1606 2474 4152 4829 5977 6222 1606
Size: 868 1678 677 1148 245 2120
Fragments arranged by size:
2120 1678 1148 868 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6184
NdeI CA'TA_TG
Cuts at: 387 3124 3334 387
Size: 2737 210 3789
Fragments arranged by size:
3789 2737 210
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 2926 4094 5639 1132
Size: 97 1697 1168 1545 2229
Fragments arranged by size:
2229 1697 1545 1168 97
PasI CC'CwG_GG
Cuts at: 1348 3358 1348
Size: 2010 4726
PciI A'CATG_T
Cuts at: 2922 5635 2922
Size: 2713 4023
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 2635 3935 1108
Size: 1206 321 1300 3909
Fragments arranged by size:
3909 1300 1206 321
PfoI T'CCnGG_A
Cuts at: 3724 3724
Size: 6736
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2789 3028 3092 2789
Size: 239 64 6433
Fragments arranged by size:
6433 239 64
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 2546 4306 2546
Size: 1760 4976
PspOMI G'GGCC_C
Cuts at: 2059 2372 2697 3191 3621 3790 2059
Size: 313 325 494 430 169 5005
Fragments arranged by size:
5005 494 430 325 313 169
PstI C_TGCA'G
Cuts at: 830 2616 2706 3884 4088 830
Size: 1786 90 1178 204 3478
Fragments arranged by size:
3478 1786 1178 204 90
PvuI CG_AT'CG
Cuts at: 2001 3328 2001
Size: 1327 5409
PvuII CAG'CTG
Cuts at: 2474 2474
Size: 6736
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SbfI CC_TGCA'GG
Cuts at: 2706 4088 2706
Size: 1382 5354
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SfcI C'TryA_G
Cuts at: 826 1045 2110 2612 2702 3880 4084 5219 5900
Size: 219 1065 502 90 1178 204 1135 681
Cuts at: 5900 6091 826
Size: 191 1471
Fragments arranged by size:
1471 1178 1135 1065 681 502 219 204
191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 2371 3788 4063 1872
Size: 499 1417 275 4545
Fragments arranged by size:
4545 1417 499 275
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 3273 3368 4840 5741 6003
Size: 197 926 186 1144 95 1472 901 262
Cuts at: 6003 820
Size: 1553
Fragments arranged by size:
1553 1472 1144 926 901 262 197 186
95
SnaBI TAC'GTA
Cuts at: 493 4527 493
Size: 4034 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6736
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SrfI GCCC'GGGC
Cuts at: 2371 2371
Size: 6736
SspI AAT'ATT
Cuts at: 5 52 2321 4660 5
Size: 47 2269 2339 2081
Fragments arranged by size:
2339 2269 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6736
StyI C'CwwG_G
Cuts at: 513 1065 3735 3766 4175 513
Size: 552 2670 31 409 3074
Fragments arranged by size:
3074 2670 552 409 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2876 4763 4780 4933 5118 5563
Size: 1255 522 774 1887 17 153 185 445
Cuts at: 5563 325
Size: 1498
Fragments arranged by size:
1887 1498 1255 774 522 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2919 4982 6694
Size: 274 80 33 51 1443 942 2063 1712
Cuts at: 6694 96
Size: 138
Fragments arranged by size:
2063 1712 1443 942 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3617 3649 3854 3886 6696 6728 3164
Size: 32 421 32 205 32 2810 32 3172
Fragments arranged by size:
3172 2810 421 205 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3856 3856
Size: 6736
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 2369 3786 4061 1870
Size: 499 1417 275 4545
Fragments arranged by size:
4545 1417 499 275
XmnI GAAnn'nnTTC
Cuts at: 2322 4865 2322
Size: 2543 4193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SwaI