Restriction map for
pFN21AE4554
(Circular) (Six-base) MAPSORT of: pFN21AE4554.seq Check: 1955 from: 1 to: 6712
pFN21AE4554 6712 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 3845 4055 1937
Size: 1299 609 210 4594
Fragments arranged by size:
4594 1299 609 210
Acc65I G'GTAC_C
Cuts at: 4033 4033
Size: 6712
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 2796 3050 4775 6159 6427 1632
Size: 812 352 254 1725 1384 268 1917
Fragments arranged by size:
1917 1725 1384 812 352 268 254
AflII C'TTAA_G
Cuts at: 820 1017 3194 820
Size: 197 2177 4338
Fragments arranged by size:
4338 2177 197
AflIII A'CryG_T
Cuts at: 1203 3817 4009 5611 6588 1203
Size: 2614 192 1602 977 1327
Fragments arranged by size:
2614 1602 1327 977 192
AgeI A'CCGG_T
Cuts at: 5543 5543
Size: 6712
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6712
AlwNI CAG_nnn'CTG
Cuts at: 4455 6027 4455
Size: 1572 5140
ApaLI G'TGCA_C
Cuts at: 4769 5925 4769
Size: 1156 5556
ApoI r'AATT_y
Cuts at: 1493 2224 2538 2878 3738 3925 3971 4021 4247
Size: 731 314 340 860 187 46 50 226
Cuts at: 4247 4399 6508 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 860 731 340 314 226 187
152 50 46
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 4037 1870
Size: 73 2094 4545
Fragments arranged by size:
4545 2094 73
BamHI G'GATC_C
Cuts at: 4042 4409 4042
Size: 367 6345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2068 2231 2498 4033
Size: 325 217 627 254 27 163 267 1535
Cuts at: 4033 5488 618
Size: 1455 1842
Fragments arranged by size:
1842 1535 1455 627 325 267 254 217
163 27
BanII G_rGCy'C
Cuts at: 1391 2187 2298 2713 4031 1391
Size: 796 111 415 1318 4072
Fragments arranged by size:
4072 1318 796 415 111
BbeI G_GCGC'C
Cuts at: 2072 2502 2072
Size: 430 6282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2763 2784 3038 3525 928
Size: 971 864 21 254 487 4115
Fragments arranged by size:
4115 971 864 487 254 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4903 4937 1903
Size: 34 2966 34 3678
Fragments arranged by size:
3678 2966 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2333 4604 5814 2333
Size: 2271 1210 3231
Fragments arranged by size:
3231 2271 1210
BclI T'GATC_A
Cuts at: 1548 3481 1548
Size: 1933 4779
BfrBI ATG'CAT
Cuts at: 2308 3590 2308
Size: 1282 5430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5323 136
Size: 107 122 71 631 605 3651 1525
Fragments arranged by size:
3651 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6707 6707
Size: 6712
BlpI GC'TnA_GC
Cuts at: 4129 4129
Size: 6712
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2958 4773 5929 1790
Size: 256 912 1815 1156 2573
Fragments arranged by size:
2573 1815 1156 912 256
BmgBI CAC'GTC
Cuts at: 1318 3818 1318
Size: 2500 4212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2763 4443 5401
Size: 416 53 83 399 24 1346 1680 958
Cuts at: 5401 442
Size: 1753
Fragments arranged by size:
1753 1680 1346 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2594 5372 1239
Size: 1355 2778 2579
Fragments arranged by size:
2778 2579 1355
Bpu10I CC'TnA_GC
Cuts at: 3085 3085
Size: 6712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4837 5702 6000 4837
Size: 865 298 5549
Fragments arranged by size:
5549 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2612 3559 3656 5375 882
Size: 617 1113 947 97 1719 2219
Fragments arranged by size:
2219 1719 1113 947 617 97
BsaAI yAC'GTr
Cuts at: 493 1204 2086 2962 3067 3576 4503 493
Size: 711 882 876 105 509 927 2702
Fragments arranged by size:
2702 927 882 876 711 509 105
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 2499 4519 4901
Size: 53 83 186 936 536 430 2020 382
Cuts at: 4901 5536 275
Size: 635 1451
Fragments arranged by size:
2020 1451 936 635 536 430 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5145 5543 5817 5964 6375 5145
Size: 398 274 147 411 5482
Fragments arranged by size:
5482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6487
Fragments arranged by size:
6487 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6712
BseYI C'CCAG_C
Cuts at: 2107 2425 2817 3405 3942 5915 6411 2107
Size: 318 392 588 537 1973 496 2408
Fragments arranged by size:
2408 1973 588 537 496 392 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2829 1163
Size: 573 1093 5046
Fragments arranged by size:
5046 1093 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 4162 4923 5951 1540
Size: 275 186 15 2146 761 1028 2301
Fragments arranged by size:
2301 2146 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2091 4773 4858 5929 2091
Size: 2682 85 1071 2874
Fragments arranged by size:
2874 2682 1071 85
BsiWI C'GTAC_G
Cuts at: 2082 2082
Size: 6712
BsmI GAATG_Cn'
Cuts at: 4223 4316 4223
Size: 93 6619
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2091 2187 2298 2713 2958 4031
Size: 399 256 45 96 111 415 245 1073
Cuts at: 4031 4773 4858 5929 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1073 1071 742 415 399 256 245
111 96 85 45
BspEI T'CCGG_A
Cuts at: 6375 6375
Size: 6712
BspHI T'CATG_A
Cuts at: 4601 6331 4601
Size: 1730 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4067 844
Size: 3223 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4599 1394
Size: 429 2776 3507
Fragments arranged by size:
3507 2776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3687 5207 5381 65
Size: 1183 2439 1520 174 1396
Fragments arranged by size:
2439 1520 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5356 5543 1700
Size: 110 3546 187 2869
Fragments arranged by size:
3546 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6712
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6712
BssSI C'ACGA_G
Cuts at: 4772 5784 4772
Size: 1012 5700
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2741 794
Size: 1947 4765
BstBI TT'CG_AA
Cuts at: 5516 5516
Size: 6712
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6712
BstYI r'GATC_y
Cuts at: 1972 3200 3764 4042 4409 4794 4811 6263 6349
Size: 1228 564 278 367 385 17 1452 86
Cuts at: 6349 6361 6707 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1228 564 385 367 346 278
86 17 12
BstZ17I GTA'TAC
Cuts at: 3237 3846 3237
Size: 609 6103
BtgI C'CryG_G
Cuts at: 513 1065 2016 2037 2405 2945 513
Size: 552 951 21 368 540 4280
Fragments arranged by size:
4280 951 552 540 368 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3321 487
Size: 683 56 2095 3878
Fragments arranged by size:
3878 2095 683 56
BtsI GCAGTG_nn'
Cuts at: 4228 5022 5042 4228
Size: 794 20 5898
Fragments arranged by size:
5898 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 2250 2793 2832 4016 2250
Size: 543 39 1184 4946
Fragments arranged by size:
4946 1184 543 39
DraIII CAC_nnn'GTG
Cuts at: 2962 2962
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5719 809
Size: 507 4403 1802
Fragments arranged by size:
4403 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 3040 3143
Size: 54 1425 50 275 18 183 1027 103
Cuts at: 3143 4159 5048 6677 8
Size: 1016 889 1629 43
Fragments arranged by size:
1629 1425 1027 1016 889 275 183 103
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 4159 1537
Size: 275 201 2146 4090
Fragments arranged by size:
4090 2146 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2366 2690 3691 4642 1353
Size: 1013 324 1001 951 3423
Fragments arranged by size:
3423 1013 1001 951 324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5299 5673 5819 387
Size: 1064 138 3710 374 146 1280
Fragments arranged by size:
3710 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 2594 2796 3050 4775 5372 6159
Size: 393 812 150 202 254 1725 597 787
Cuts at: 6159 6427 1239
Size: 268 1524
Fragments arranged by size:
1725 1524 812 787 597 393 268 254
202 150
EcoNI CCTnn'n_nnAGG
Cuts at: 3672 3672
Size: 6712
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
FspI TGC'GCA
Cuts at: 5218 5218
Size: 6712
HaeII r_GCGC'y
Cuts at: 1678 2072 2502 5859 1678
Size: 394 430 3357 2531
Fragments arranged by size:
3357 2531 430 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2607 2639 5353 5385 5427
Size: 32 135 32 1035 32 2714 32 42
Cuts at: 5427 5459 1373
Size: 32 2626
Fragments arranged by size:
2714 2626 1035 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 3858 4056 4302 669
Size: 988 281 813 1107 198 246 3079
Fragments arranged by size:
3079 1107 988 813 281 246 198
HindIII A'AGCT_T
Cuts at: 748 2762 748
Size: 2014 4698
HpaI GTT'AAC
Cuts at: 3858 4302 3858
Size: 444 6268
KasI G'GCGC_C
Cuts at: 2068 2498 2068
Size: 430 6282
KpnI G_GTAC'C
Cuts at: 4037 4037
Size: 6712
MfeI C'AATT_G
Cuts at: 3951 4311 3951
Size: 360 6352
MluI A'CGCG_T
Cuts at: 4009 6588 4009
Size: 2579 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 3618 5826 6010 1305
Size: 819 1494 2208 184 2007
Fragments arranged by size:
2208 2007 1494 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3042 10
Size: 54 1425 343 1210 3680
Fragments arranged by size:
3680 1425 1210 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3336 3920 4670 5029 5188 518
Size: 648 2170 584 750 359 159 2042
Fragments arranged by size:
2170 2042 750 648 584 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2039 2142 2464 3280 4128 4805 5953
Size: 412 21 103 322 816 848 677 1148
Cuts at: 5953 6198 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 848 816 677 412 322 245
103 21
NarI GG'CG_CC
Cuts at: 2069 2499 2069
Size: 430 6282
NcoI C'CATG_G
Cuts at: 513 1065 2405 2945 513
Size: 552 1340 540 4280
Fragments arranged by size:
4280 1340 552 540
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6712
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 2013 4159 2013
Size: 2146 4566
NsiI A_TGCA'T
Cuts at: 2310 3592 2310
Size: 1282 5430
NspI r_CATG'y
Cuts at: 1132 1229 2308 3889 4070 5615 1132
Size: 97 1079 1581 181 1545 2229
Fragments arranged by size:
2229 1581 1545 1079 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6712
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2518 2951 3576 3938 1108
Size: 1410 433 625 362 3882
Fragments arranged by size:
3882 1410 625 433 362
PfoI T'CCnGG_A
Cuts at: 2719 2719
Size: 6712
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6712
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PstI C_TGCA'G
Cuts at: 830 4064 830
Size: 3234 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2464 3280 2464
Size: 816 5896
SacII CC_GC'GG
Cuts at: 2019 2040 2019
Size: 21 6691
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SfcI C'TryA_G
Cuts at: 826 1045 3224 4060 5195 5876 6067 826
Size: 219 2179 836 1135 681 191 1471
Fragments arranged by size:
2179 1471 1135 836 681 219 191
SfoI GGC'GCC
Cuts at: 2070 2500 2070
Size: 430 6282
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 4039 1872
Size: 2167 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3194 4816 5717 5979 820
Size: 197 926 1251 1622 901 262 1553
Fragments arranged by size:
1622 1553 1251 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3067 4503 493
Size: 2574 1436 2702
Fragments arranged by size:
2702 2574 1436
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 3889 4070 1132
Size: 2757 181 3774
Fragments arranged by size:
3774 2757 181
SspI AAT'ATT
Cuts at: 5 52 2386 3503 3514 4636 5
Size: 47 2334 1117 11 1122 2081
Fragments arranged by size:
2334 2081 1122 1117 47 11
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6712
StyI C'CwwG_G
Cuts at: 513 1065 2405 2945 4151 513
Size: 552 1340 540 1206 3074
Fragments arranged by size:
3074 1340 1206 552 540
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 4739 4756 4909 5094 5539 325
Size: 1255 1215 1944 17 153 185 445 1498
Fragments arranged by size:
1944 1498 1255 1215 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4958 6670 96
Size: 274 80 33 51 1443 2981 1712 138
Fragments arranged by size:
2981 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6672 6704 6672
Size: 32 6680
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6712
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3939 3939
Size: 6712
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 4037 1870
Size: 2167 4545
XmnI GAAnn'nnTTC
Cuts at: 2330 2728 3264 4841 2330
Size: 398 536 1577 4201
Fragments arranged by size:
4201 1577 536 398
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BsmBI BstXI Bsu36I EcoICRI EcoO109I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI