Restriction map for
pFN21AE4552
(Circular) (Six-base) MAPSORT of: pFN21AE4552.seq Check: 8423 from: 1 to: 6691
pFN21AE4552 6691 bp
With 182 enzymes: SgfI *
March 28, 2012 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3237 3634 3237
Size: 397 6294
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3533 4501 278
Size: 53 83 186 936 1997 968 2468
Fragments arranged by size:
2468 1997 968 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4034 1937
Size: 2097 4594
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 2534 2564 3129 3461 4754 6138 6406
Size: 642 260 30 565 332 1293 1384 268
Cuts at: 6406 1632
Size: 1917
Fragments arranged by size:
1917 1384 1293 642 565 332 268 260
30
AfeI AGC'GCT
Cuts at: 2186 2601 2186
Size: 415 6276
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6494
AflIII A'CryG_T
Cuts at: 1203 5590 6567 1203
Size: 4387 977 1327
Fragments arranged by size:
4387 1327 977
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 3121 5420 3121
Size: 2299 4392
AleI CACnn'nnGTG
Cuts at: 1166 3656 1166
Size: 2490 4201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2921 1184
Size: 1737 4954
AlwNI CAG_nnn'CTG
Cuts at: 3607 4434 6006 3607
Size: 827 1572 4292
Fragments arranged by size:
4292 1572 827
ApaI G_GGCC'C
Cuts at: 3665 3665
Size: 6691
ApaLI G'TGCA_C
Cuts at: 4748 5904 4748
Size: 1156 5535
ApoI r'AATT_y
Cuts at: 1493 2225 2301 4000 4226 4378 6487 1493
Size: 732 76 1699 226 152 2109 1697
Fragments arranged by size:
2109 1699 1697 732 226 152 76
AseI AT'TA_AT
Cuts at: 160 1032 5245 160
Size: 872 4213 1606
Fragments arranged by size:
4213 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 2439 3062 3694 3920 4016 1870
Size: 73 496 623 632 226 96 4545
Fragments arranged by size:
4545 632 623 496 226 96 73
BamHI G'GATC_C
Cuts at: 4021 4388 4021
Size: 367 6324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 3096 3578 4012 5467
Size: 325 217 627 237 1072 482 434 1455
Cuts at: 5467 618
Size: 1842
Fragments arranged by size:
1842 1455 1072 627 482 434 325 237
217
BanII G_rGCy'C
Cuts at: 1391 2070 2721 2778 3665 4010 1391
Size: 679 651 57 887 345 4072
Fragments arranged by size:
4072 887 679 651 345 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5720
BbvCI CC'TCA_GC
Cuts at: 3197 3197
Size: 6691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4882 4916 1903
Size: 34 2945 34 3678
Fragments arranged by size:
3678 2945 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2273 2384 4583 5793 2273
Size: 111 2199 1210 3171
Fragments arranged by size:
3171 2199 1210 111
BclI T'GATC_A
Cuts at: 1548 2995 1548
Size: 1447 5244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3814 6686 3814
Size: 2872 3819
BlpI GC'TnA_GC
Cuts at: 4108 4108
Size: 6691
Bme1580I G_kGCm'C
Cuts at: 1790 3480 3581 3665 4752 5908 1790
Size: 1690 101 84 1087 1156 2573
Fragments arranged by size:
2573 1690 1156 1087 101 84
BmgBI CAC'GTC
Cuts at: 1318 3475 3636 3840 3883 1318
Size: 2157 161 204 43 4126
Fragments arranged by size:
4126 2157 204 161 43
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3491 3659 4422
Size: 416 53 83 399 24 2074 168 763
Cuts at: 4422 5380 442
Size: 958 1753
Fragments arranged by size:
2074 1753 958 763 416 399 168 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6691
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3136 3136
Size: 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2682 2699 3413 5351 1239
Size: 1443 17 714 1938 2579
Fragments arranged by size:
2579 1938 1443 714 17
Bpu10I CC'TnA_GC
Cuts at: 3197 3197
Size: 6691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2766 3331 4816 5681 5979 2766
Size: 565 1485 865 298 3478
Fragments arranged by size:
3478 1485 865 565 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2764 3051 5354 882
Size: 617 1265 287 2303 2219
Fragments arranged by size:
2303 2219 1265 617 287
BsaAI yAC'GTr
Cuts at: 493 1204 3729 4482 493
Size: 711 2525 753 2702
Fragments arranged by size:
2702 2525 753 711
BsaBI GATnn'nnATC
Cuts at: 2378 6685 2378
Size: 4307 2384
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3530 3546 4498 4880
Size: 53 83 186 936 1997 16 952 382
Cuts at: 4880 5515 275
Size: 635 1451
Fragments arranged by size:
1997 1451 952 936 635 382 186 83
53 16
BsaWI w'CCGG_w
Cuts at: 2457 5124 5522 5796 5943 6354 2457
Size: 2667 398 274 147 411 2794
Fragments arranged by size:
2794 2667 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 2726 2756 3020
Size: 30 165 30 654 30 639 30 264
Cuts at: 3020 3050 1178
Size: 30 4819
Fragments arranged by size:
4819 654 639 264 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 3109 3251 1186
Size: 996 927 142 4626
Fragments arranged by size:
4626 996 927 142
BseYI C'CCAG_C
Cuts at: 2181 2602 2819 2953 3574 5894 6390 2181
Size: 421 217 134 621 2320 496 2482
Fragments arranged by size:
2482 2320 621 496 421 217 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 3803 1163
Size: 573 798 1269 4051
Fragments arranged by size:
4051 1269 798 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 4141 4902 5930 1540
Size: 275 186 21 2119 761 1028 2301
Fragments arranged by size:
2301 2119 1028 761 275 186 21
BsiHKAI G_wGCw'C
Cuts at: 3687 4752 4837 5908 3687
Size: 1065 85 1071 4470
Fragments arranged by size:
4470 1071 1065 85
BsmI GAATG_Cn'
Cuts at: 2041 4202 4295 2041
Size: 2161 93 4437
Fragments arranged by size:
4437 2161 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2120 2393 3830 2120
Size: 273 1437 4981
Fragments arranged by size:
4981 1437 273
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2721 2778 3480 3581 3665 3687
Size: 399 280 651 57 702 101 84 22
Cuts at: 3687 4010 4752 4837 5908 1391
Size: 323 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 651 399 323 280
101 85 84 57 22
BspEI T'CCGG_A
Cuts at: 2457 6354 2457
Size: 3897 2794
BspHI T'CATG_A
Cuts at: 3204 4580 6310 3204
Size: 1376 1730 3585
Fragments arranged by size:
3585 1730 1376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 2454 3237 3634 4046 844
Size: 1298 312 783 397 412 3489
Fragments arranged by size:
3489 1298 783 412 397 312
BsrBI CCG'CTC
Cuts at: 1394 1823 3020 4578 1394
Size: 429 1197 1558 3507
Fragments arranged by size:
3507 1558 1197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3948 5186 5360 65
Size: 1183 2700 1238 174 1396
Fragments arranged by size:
2700 1396 1238 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3099 5335 5522 1700
Size: 110 1289 2236 187 2869
Fragments arranged by size:
2869 2236 1289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6691
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 4751 5763 4751
Size: 1012 5679
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6691
BstBI TT'CG_AA
Cuts at: 3774 5495 3774
Size: 1721 4970
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6691
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2677 2731 3466 3658 2113
Size: 564 54 735 192 5146
Fragments arranged by size:
5146 735 564 192 54
BstYI r'GATC_y
Cuts at: 1972 3814 4021 4388 4773 4790 6242 6328 6340
Size: 1842 207 367 385 17 1452 86 12
Cuts at: 6340 6686 1972
Size: 346 1977
Fragments arranged by size:
1977 1842 1452 385 367 346 207 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3965 3965
Size: 6691
BtgI C'CryG_G
Cuts at: 513 1065 2004 3126 3465 513
Size: 552 939 1122 339 3739
Fragments arranged by size:
3739 1122 939 552 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2656 487
Size: 683 56 1430 4522
Fragments arranged by size:
4522 1430 683 56
BtsI GCAGTG_nn'
Cuts at: 4207 5001 5021 4207
Size: 794 20 5877
Fragments arranged by size:
5877 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6656
DraI TTT'AAA
Cuts at: 3995 3995
Size: 6691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5698 809
Size: 507 4382 1802
Fragments arranged by size:
4382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2334 2667
Size: 54 1425 50 275 18 177 327 333
Cuts at: 2667 3456 3648 4138 5027 6656 8
Size: 789 192 490 889 1629 43
Fragments arranged by size:
1629 1425 889 789 490 333 327 275
192 177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4138 1537
Size: 275 2326 4090
Fragments arranged by size:
4090 2326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 3105 3185 3335 4621 1353
Size: 1484 268 80 150 1286 3423
Fragments arranged by size:
3423 1484 1286 268 150 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3932 5278 5652 5798 387
Size: 1064 138 2343 1346 374 146 1280
Fragments arranged by size:
2343 1346 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2274 2534 2564 2682 2699 3129 3413
Size: 393 642 260 30 118 17 430 284
Cuts at: 3413 3461 4754 5351 6138 6406 1239
Size: 48 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 787 642 597 430 393 284
268 260 118 48 30 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2266 3760 2266
Size: 1494 5197
EcoO109I rG'GnC_Cy
Cuts at: 2233 2258 2345 2443 3189 2233
Size: 25 87 98 746 5735
Fragments arranged by size:
5735 746 98 87 25
EcoRI G'AATT_C
Cuts at: 2225 4000 4378 2225
Size: 1775 378 4538
Fragments arranged by size:
4538 1775 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3208 3325 3208
Size: 117 6574
FspI TGC'GCA
Cuts at: 3233 5197 3233
Size: 1964 4727
HaeII r_GCGC'y
Cuts at: 1678 2188 2603 2665 2742 2826 5838 1678
Size: 510 415 62 77 84 3012 2531
Fragments arranged by size:
3012 2531 510 415 84 77 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2087 2089 2119 2726
Size: 32 135 32 485 30 2 30 607
Cuts at: 2726 2758 2963 2995 3107 3139 5332 5364 5406
Size: 32 205 32 112 32 2193 32 42
Cuts at: 5406 5438 1373
Size: 32 2626
Fragments arranged by size:
2626 2193 607 485 205 135 112 42
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4035 4281 669
Size: 988 281 2097 246 3079
Fragments arranged by size:
3079 2097 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2546 748
Size: 1798 4893
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3567 3837 5805 5989 1305
Size: 2262 270 1968 184 2007
Fragments arranged by size:
2262 2007 1968 270 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2336 2669 3458 3650 10
Size: 54 1425 343 504 333 789 192 3051
Fragments arranged by size:
3051 1425 789 504 343 333 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2372 2528 3656 3680 4649 5008
Size: 648 864 342 156 1128 24 969 359
Cuts at: 5008 5167 518
Size: 159 2042
Fragments arranged by size:
2042 1128 969 864 648 359 342 159
156 24
MspA1I CmG'CkG
Cuts at: 1606 2213 2727 3156 4107 4784 5932 6177 1606
Size: 607 514 429 951 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 607 514 429 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3126 3465 513
Size: 552 939 1122 339 3739
Fragments arranged by size:
3739 1122 939 552 339
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6691
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 2494 3037 4049 5594 1132
Size: 97 1265 543 1012 1545 2229
Fragments arranged by size:
2229 1545 1265 1012 543 97
PasI CC'CwG_GG
Cuts at: 1348 3607 1348
Size: 2259 4432
PciI A'CATG_T
Cuts at: 5590 5590
Size: 6691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6691
PfoI T'CCnGG_A
Cuts at: 2306 2306
Size: 6691
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4760 4792 6273 6305 4760
Size: 32 1481 32 5146
Fragments arranged by size:
5146 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2258 2345 2443 3189 2233
Size: 25 87 98 746 5735
Fragments arranged by size:
5735 746 98 87 25
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 4261 4261
Size: 6691
PspOMI G'GGCC_C
Cuts at: 3661 3661
Size: 6691
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 6659
PstI C_TGCA'G
Cuts at: 830 2056 3512 3645 4043 830
Size: 1226 1456 133 398 3478
Fragments arranged by size:
3478 1456 1226 398 133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
PvuII CAG'CTG
Cuts at: 2213 3156 2213
Size: 943 5748
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 6691
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SbfI CC_TGCA'GG
Cuts at: 3645 4043 3645
Size: 398 6293
ScaI AGT'ACT
Cuts at: 4939 6651 4939
Size: 1712 4979
SfcI C'TryA_G
Cuts at: 826 1045 2052 3508 3641 3734 4039 5174 5855
Size: 219 1007 1456 133 93 305 1135 681
Cuts at: 5855 6046 826
Size: 191 1471
Fragments arranged by size:
1471 1456 1135 1007 681 305 219 191
133 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 2441 4018 1872
Size: 569 1577 4545
Fragments arranged by size:
4545 1577 569
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2781 3310 4795 5696 5958 820
Size: 197 926 838 529 1485 901 262 1553
Fragments arranged by size:
1553 1485 926 901 838 529 262 197
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 4049 1132
Size: 2917 3774
SspI AAT'ATT
Cuts at: 5 52 4615 5
Size: 47 4563 2081
Fragments arranged by size:
4563 2081 47
StuI AGG'CCT
Cuts at: 1517 3446 1517
Size: 1929 4762
StyI C'CwwG_G
Cuts at: 513 1065 2004 3126 3465 4130 513
Size: 552 939 1122 339 665 3074
Fragments arranged by size:
3074 1122 939 665 552 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 3205 4718 4735 4888 5073 5518
Size: 1255 428 1197 1513 17 153 185 445
Cuts at: 5518 325
Size: 1498
Fragments arranged by size:
1513 1498 1255 1197 445 428 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3276 3519 4937
Size: 274 80 33 51 1443 1299 243 1418
Cuts at: 4937 6649 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1418 1299 274 243 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2862 2894 3020 3052 6651 6683 2862
Size: 32 126 32 3599 32 2870
Fragments arranged by size:
3599 2870 126 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3215 1316
Size: 1899 4792
XbaI T'CTAG_A
Cuts at: 4027 4027
Size: 6691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 3589 3901 2728
Size: 861 312 5518
Fragments arranged by size:
5518 861 312
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 2439 4016 1870
Size: 569 1577 4545
Fragments arranged by size:
4545 1577 569
XmnI GAAnn'nnTTC
Cuts at: 4820 4820
Size: 6691
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3531 4499 276
Size: 53 83 186 936 1997 968 2468
Fragments arranged by size:
2468 1997 968 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI