Restriction map for
pFN21AE4551
(Circular) (Six-base) MAPSORT of: pFN21AE4551.seq Check: 1127 from: 1 to: 6688
pFN21AE4551 6688 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3049 3217 3049
Size: 168 6520
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4498 278
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2568 3341 3922 4031 1937
Size: 631 773 581 109 4594
Fragments arranged by size:
4594 773 631 581 109
Acc65I G'GTAC_C
Cuts at: 3161 4009 3161
Size: 848 5840
AclI AA'CG_TT
Cuts at: 1563 4815 5188 1563
Size: 3252 373 3063
Fragments arranged by size:
3252 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2781 3093 3333 3698 4751 6135 6403 1632
Size: 1149 312 240 365 1053 1384 268 1917
Fragments arranged by size:
1917 1384 1149 1053 365 312 268 240
AflII C'TTAA_G
Cuts at: 820 1017 3394 820
Size: 197 2377 4114
Fragments arranged by size:
4114 2377 197
AflIII A'CryG_T
Cuts at: 1203 5587 6564 1203
Size: 4384 977 1327
Fragments arranged by size:
4384 1327 977
AgeI A'CCGG_T
Cuts at: 5519 5519
Size: 6688
AhdI GACnn_n'nnGTC
Cuts at: 5417 5417
Size: 6688
AleI CACnn'nnGTG
Cuts at: 1166 3053 1166
Size: 1887 4801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2552 1184
Size: 1368 5320
AlwNI CAG_nnn'CTG
Cuts at: 2507 2716 2864 3622 3676 3912 4431 6003 2507
Size: 209 148 758 54 236 519 1572 3192
Fragments arranged by size:
3192 1572 758 519 236 209 148 54
ApaI G_GGCC'C
Cuts at: 2501 3371 2501
Size: 870 5818
ApaLI G'TGCA_C
Cuts at: 4745 5901 4745
Size: 1156 5532
ApoI r'AATT_y
Cuts at: 1493 3997 4223 4375 6484 1493
Size: 2504 226 152 2109 1697
Fragments arranged by size:
2504 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5242 160
Size: 872 4210 1606
Fragments arranged by size:
4210 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
AvaI C'yCGr_G
Cuts at: 1870 1943 2817 3688 4013 1870
Size: 73 874 871 325 4545
Fragments arranged by size:
4545 874 871 325 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2064 2097 2064
Size: 33 6655
BamHI G'GATC_C
Cuts at: 2582 4018 4385 2582
Size: 1436 367 4885
Fragments arranged by size:
4885 1436 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2641 2927 3022 3061 3139
Size: 325 217 627 854 286 95 39 78
Cuts at: 3139 3161 3600 4009 5464 618
Size: 22 439 409 1455 1842
Fragments arranged by size:
1842 1455 854 627 439 409 325 286
217 95 78 39 22
BanII G_rGCy'C
Cuts at: 1391 2501 3039 3371 4007 1391
Size: 1110 538 332 636 4072
Fragments arranged by size:
4072 1110 636 538 332
BbeI G_GCGC'C
Cuts at: 3026 3026
Size: 6688
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2471 928
Size: 971 572 5145
Fragments arranged by size:
5145 971 572
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4879 4913 1903
Size: 34 2942 34 3678
Fragments arranged by size:
3678 2942 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2160 2379 4580 5790 2160
Size: 219 2201 1210 3058
Fragments arranged by size:
3058 2201 1210 219
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6688
BfrBI ATG'CAT
Cuts at: 3530 3530
Size: 6688
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2302 2424 5299
Size: 107 122 71 631 605 630 122 2875
Cuts at: 5299 136
Size: 1525
Fragments arranged by size:
2875 1525 631 630 605 122 122 107
71
BglII A'GATC_T
Cuts at: 3585 6683 3585
Size: 3098 3590
BlpI GC'TnA_GC
Cuts at: 2513 2617 4105 2513
Size: 104 1488 5096
Fragments arranged by size:
5096 1488 104
Bme1580I G_kGCm'C
Cuts at: 1790 2501 2546 2824 2943 3371 4749 5905 1790
Size: 711 45 278 119 428 1378 1156 2573
Fragments arranged by size:
2573 1378 1156 711 428 278 119 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 2659 2852
Size: 416 53 83 399 24 1078 164 193
Cuts at: 2852 2912 3883 4419 5377 442
Size: 60 971 536 958 1753
Fragments arranged by size:
1753 1078 971 958 536 416 399 193
164 83 60 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2206 2714 2867 3171 5348 1239
Size: 967 508 153 304 2177 2579
Fragments arranged by size:
2579 2177 967 508 304 153
Bpu10I CC'TnA_GC
Cuts at: 3306 3306
Size: 6688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2095 2192 4813 5678 5976 2095
Size: 97 2621 865 298 2807
Fragments arranged by size:
2807 2621 865 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 3272 5351 882
Size: 617 940 833 2079 2219
Fragments arranged by size:
2219 2079 940 833 617
BsaAI yAC'GTr
Cuts at: 493 1204 4479 493
Size: 711 3275 2702
Fragments arranged by size:
3275 2702 711
BsaBI GATnn'nnATC
Cuts at: 6682 6682
Size: 6688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3023 4495 4877 5512
Size: 53 83 186 936 1490 1472 382 635
Cuts at: 5512 275
Size: 1451
Fragments arranged by size:
1490 1472 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3515 5121 5519 5793 5940 6351 3515
Size: 1606 398 274 147 411 3852
Fragments arranged by size:
3852 1606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2179 2209 1178
Size: 30 165 30 776 30 5657
Fragments arranged by size:
5657 776 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3959 1186
Size: 2773 3915
BseYI C'CCAG_C
Cuts at: 2539 3615 3641 3646 3750 5891 6387 2539
Size: 1076 26 5 104 2141 496 2840
Fragments arranged by size:
2840 2141 1076 496 104 26 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3072 3077 3116 3234 3650 1163
Size: 573 1336 5 39 118 416 4201
Fragments arranged by size:
4201 1336 573 416 118 39 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4138 4899 5927 1540
Size: 275 186 2137 761 1028 2301
Fragments arranged by size:
2301 2137 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3119 4749 4834 5905 3119
Size: 1630 85 1071 3902
Fragments arranged by size:
3902 1630 1071 85
BsmI GAATG_Cn'
Cuts at: 4199 4292 4199
Size: 93 6595
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2501 2546 2824 2943 3039 3119 3371
Size: 399 711 45 278 119 96 80 252
Cuts at: 3371 4007 4749 4834 5905 1391
Size: 636 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 711 636 399 278 252
119 96 85 80 45
BspEI T'CCGG_A
Cuts at: 6351 6351
Size: 6688
BspHI T'CATG_A
Cuts at: 4577 6307 4577
Size: 1730 4958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2296 3049 3217 3778 3973 4043 844
Size: 1452 753 168 561 195 70 3489
Fragments arranged by size:
3489 1452 753 561 195 168 70
BsrBI CCG'CTC
Cuts at: 1394 1823 4575 1394
Size: 429 2752 3507
Fragments arranged by size:
3507 2752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 5183 5357 65
Size: 1183 1293 2642 174 1396
Fragments arranged by size:
2642 1396 1293 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5332 5519 1700
Size: 110 3522 187 2869
Fragments arranged by size:
3522 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6688
BssSI C'ACGA_G
Cuts at: 4748 5760 4748
Size: 1012 5676
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3508 3936 794
Size: 2714 428 3546
Fragments arranged by size:
3546 2714 428
BstBI TT'CG_AA
Cuts at: 5492 5492
Size: 6688
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6688
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3733 2011
Size: 1722 4966
BstYI r'GATC_y
Cuts at: 1972 2008 2582 3495 3585 4018 4385 4770 4787
Size: 36 574 913 90 433 367 385 17
Cuts at: 4787 6239 6325 6337 6683 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 913 574 433 385 367 346
90 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2553 513
Size: 552 939 549 4648
Fragments arranged by size:
4648 939 552 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2558 3576 487
Size: 683 56 1332 1018 3599
Fragments arranged by size:
3599 1332 1018 683 56
BtsI GCAGTG_nn'
Cuts at: 2862 3084 3744 4204 4998 5018 2862
Size: 222 660 460 794 20 4532
Fragments arranged by size:
4532 794 660 460 222 20
ClaI AT'CG_AT
Cuts at: 1554 3439 1554
Size: 1885 4803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6653
DraI TTT'AAA
Cuts at: 3992 3992
Size: 6688
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5695 809
Size: 507 4379 1802
Fragments arranged by size:
4379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2977 4135 5024
Size: 54 1425 50 275 18 1147 1158 889
Cuts at: 5024 6653 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1158 1147 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4135 1537
Size: 275 2323 4090
Fragments arranged by size:
4090 2323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 3401 4618 1353
Size: 1082 966 1217 3423
Fragments arranged by size:
3423 1217 1082 966
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5275 5649 5795 387
Size: 1064 138 3686 374 146 1280
Fragments arranged by size:
3686 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2714 2781 2867 3093 3171 3333
Size: 393 574 508 67 86 226 78 162
Cuts at: 3333 3698 4751 5348 6135 6403 1239
Size: 365 1053 597 787 268 1524
Fragments arranged by size:
1524 1053 787 597 574 508 393 365
268 226 162 86 78 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2137 3785 2137
Size: 1648 5040
EcoO109I rG'GnC_Cy
Cuts at: 2497 2959 3368 2497
Size: 462 409 5817
Fragments arranged by size:
5817 462 409
EcoRI G'AATT_C
Cuts at: 3997 4375 3997
Size: 378 6310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 3797 3892 2464
Size: 1333 95 5260
Fragments arranged by size:
5260 1333 95
FspI TGC'GCA
Cuts at: 5194 5194
Size: 6688
HaeII r_GCGC'y
Cuts at: 1678 3026 5835 1678
Size: 1348 2809 2531
Fragments arranged by size:
2809 2531 1348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2179 2211 3169 3201 3314
Size: 32 135 32 607 32 958 32 113
Cuts at: 3314 3346 3575 3607 5329 5361 5403 5435 1373
Size: 32 229 32 1722 32 42 32 2626
Fragments arranged by size:
2626 1722 958 607 229 135 113 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 3183 4032 4278 669
Size: 988 281 382 863 849 246 3079
Fragments arranged by size:
3079 988 863 849 382 281 246
HindIII A'AGCT_T
Cuts at: 748 2446 748
Size: 1698 4990
HpaI GTT'AAC
Cuts at: 4278 4278
Size: 6688
KasI G'GCGC_C
Cuts at: 3022 3022
Size: 6688
KpnI G_GTAC'C
Cuts at: 3165 4013 3165
Size: 848 5840
MfeI C'AATT_G
Cuts at: 3222 4287 3222
Size: 1065 5623
MluI A'CGCG_T
Cuts at: 6564 6564
Size: 6688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5802 5986 1305
Size: 4497 184 2007
Fragments arranged by size:
4497 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2979 10
Size: 54 1425 343 1147 3719
Fragments arranged by size:
3719 1425 1147 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 3053 3122 4646 5005 5164 518
Size: 648 1392 495 69 1524 359 159 2042
Fragments arranged by size:
2042 1524 1392 648 495 359 159 69
MspA1I CmG'CkG
Cuts at: 1606 2146 2975 3248 3619 3658 3796 4104 4781
Size: 540 829 273 371 39 138 308 677
Cuts at: 4781 5929 6174 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 540 371 308 273
245 138 39
NarI GG'CG_CC
Cuts at: 3023 3023
Size: 6688
NcoI C'CATG_G
Cuts at: 513 1065 2004 2553 513
Size: 552 939 549 4648
Fragments arranged by size:
4648 939 552 549
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6688
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6688
NotI GC'GGCC_GC
Cuts at: 4135 4135
Size: 6688
NsiI A_TGCA'T
Cuts at: 3532 3532
Size: 6688
NspI r_CATG'y
Cuts at: 1132 1229 4046 5591 1132
Size: 97 2817 1545 2229
Fragments arranged by size:
2817 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3364 1348
Size: 2016 4672
PciI A'CATG_T
Cuts at: 5587 5587
Size: 6688
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2133 2507 1108
Size: 1025 374 5289
Fragments arranged by size:
5289 1025 374
PfoI T'CCnGG_A
Cuts at: 2585 2585
Size: 6688
PmeI GTTT'AAAC
Cuts at: 3992 3992
Size: 6688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4789 6270 6302 4757
Size: 32 1481 32 5143
Fragments arranged by size:
5143 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6688
PsiI TTA'TAA
Cuts at: 4258 4258
Size: 6688
PspOMI G'GGCC_C
Cuts at: 2497 3367 2497
Size: 870 5818
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3343 3375 3343
Size: 32 6656
PstI C_TGCA'G
Cuts at: 830 2883 2956 3034 3112 3214 3504 4040 830
Size: 2053 73 78 78 102 290 536 3478
Fragments arranged by size:
3478 2053 536 290 102 78 78 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6688
PvuII CAG'CTG
Cuts at: 2146 2975 3248 3658 2146
Size: 829 273 410 5176
Fragments arranged by size:
5176 829 410 273
SalI G'TCGA_C
Cuts at: 1936 4030 1936
Size: 2094 4594
SbfI CC_TGCA'GG
Cuts at: 2883 3504 4040 2883
Size: 621 536 5531
Fragments arranged by size:
5531 621 536
ScaI AGT'ACT
Cuts at: 4936 6648 4936
Size: 1712 4976
SexAI A'CCwGG_T
Cuts at: 2637 3013 2637
Size: 376 6312
SfcI C'TryA_G
Cuts at: 826 1045 2879 2952 2991 3030 3108 3195 3210
Size: 219 1834 73 39 39 78 87 15
Cuts at: 3210 3264 3500 4036 5171 5852 6043 826
Size: 54 236 536 1135 681 191 1471
Fragments arranged by size:
1834 1471 1135 681 536 236 219 191
87 78 73 54 39 39 15
SfoI GGC'GCC
Cuts at: 3024 3024
Size: 6688
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6688
SmaI CCC'GGG
Cuts at: 1872 4015 1872
Size: 2143 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2110 2207 3394 4792 5693 5955
Size: 197 926 167 97 1187 1398 901 262
Cuts at: 5955 820
Size: 1553
Fragments arranged by size:
1553 1398 1187 926 901 262 197 167
97
SnaBI TAC'GTA
Cuts at: 493 4479 493
Size: 3986 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6688
SphI G_CATG'C
Cuts at: 1132 4046 1132
Size: 2914 3774
SspI AAT'ATT
Cuts at: 5 52 4612 5
Size: 47 4560 2081
Fragments arranged by size:
4560 2081 47
StuI AGG'CCT
Cuts at: 1517 3780 1517
Size: 2263 4425
StyI C'CwwG_G
Cuts at: 513 1065 2004 2553 2676 3553 3567 3781 4127
Size: 552 939 549 123 877 14 214 346
Cuts at: 4127 513
Size: 3074
Fragments arranged by size:
3074 939 877 552 549 346 214 123
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3262 3764 4715 4732 4885 5070 5515
Size: 1255 1682 502 951 17 153 185 445
Cuts at: 5515 325
Size: 1498
Fragments arranged by size:
1682 1498 1255 951 502 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4934 6646 96
Size: 274 80 33 51 1443 2957 1712 138
Fragments arranged by size:
2957 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2349 2381 6648 6680 2349
Size: 32 4267 32 2357
Fragments arranged by size:
4267 2357 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3470 3544 1316
Size: 2154 74 4460
Fragments arranged by size:
4460 2154 74
XbaI T'CTAG_A
Cuts at: 4024 4024
Size: 6688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 2427 2744 3397 2099
Size: 328 317 653 5390
Fragments arranged by size:
5390 653 328 317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6688
XmaI C'CCGG_G
Cuts at: 1870 4013 1870
Size: 2143 4545
XmnI GAAnn'nnTTC
Cuts at: 4817 4817
Size: 6688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4496 276
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PmlI PpuMI PspXI RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI