Restriction map for
pFN21AE4550
(Circular) (Six-base) MAPSORT of: pFN21AE4550.seq Check: 8200 from: 1 to: 6676
pFN21AE4550 6676 bp
With 182 enzymes: SgfI *
March 28, 2012 14:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4486 278
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2063 2959 4019 1937
Size: 126 896 1060 4594
Fragments arranged by size:
4594 1060 896 126
Acc65I G'GTAC_C
Cuts at: 2122 3997 2122
Size: 1875 4801
AclI AA'CG_TT
Cuts at: 1563 2217 4803 5176 1563
Size: 654 2586 373 3063
Fragments arranged by size:
3063 2586 654 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2011 2098 2277 2634 2711 2722 2817 3177
Size: 379 87 179 357 77 11 95 360
Cuts at: 3177 3636 3689 4739 6123 6391 1632
Size: 459 53 1050 1384 268 1917
Fragments arranged by size:
1917 1384 1050 459 379 360 357 268
179 95 87 77 53 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6479
AflIII A'CryG_T
Cuts at: 1203 2887 5575 6552 1203
Size: 1684 2688 977 1327
Fragments arranged by size:
2688 1684 1327 977
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 5405 5405
Size: 6676
AleI CACnn'nnGTG
Cuts at: 1166 2348 1166
Size: 1182 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6676
AlwNI CAG_nnn'CTG
Cuts at: 3431 3853 4419 5991 3431
Size: 422 566 1572 4116
Fragments arranged by size:
4116 1572 566 422
ApaLI G'TGCA_C
Cuts at: 4733 5889 4733
Size: 1156 5520
ApoI r'AATT_y
Cuts at: 1493 2714 2903 3014 3294 3398 3985 4211 4363
Size: 1221 189 111 280 104 587 226 152
Cuts at: 4363 6472 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1221 587 280 226 189 152
111 104
AseI AT'TA_AT
Cuts at: 160 1032 3906 5230 160
Size: 872 2874 1324 1606
Fragments arranged by size:
2874 1606 1324 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 3260 4001 1870
Size: 73 1317 741 4545
Fragments arranged by size:
4545 1317 741 73
AvrII C'CTAG_G
Cuts at: 3271 3271
Size: 6676
BamHI G'GATC_C
Cuts at: 4006 4373 4006
Size: 367 6309
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3997 5452 618
Size: 325 217 627 335 1875 1455 1842
Fragments arranged by size:
1875 1842 1455 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 3493 3995 1391
Size: 2102 502 4072
Fragments arranged by size:
4072 2102 502
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2306 2458 3831 928
Size: 971 407 152 1373 3773
Fragments arranged by size:
3773 1373 971 407 152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4867 4901 1903
Size: 34 2930 34 3678
Fragments arranged by size:
3678 2930 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4568 5778 4568
Size: 1210 5466
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5287 136
Size: 107 122 71 631 605 3615 1525
Fragments arranged by size:
3615 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6671 6671
Size: 6676
BlpI GC'TnA_GC
Cuts at: 3843 4093 3843
Size: 250 6426
Bme1580I G_kGCm'C
Cuts at: 1790 4737 5893 1790
Size: 2947 1156 2573
Fragments arranged by size:
2947 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4407 5365 442
Size: 416 53 83 399 24 2990 958 1753
Fragments arranged by size:
2990 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3593 1056
Size: 2537 4139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3534 3534
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5336 1239
Size: 4097 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2771 2833 2984 4801 5666 5964 2771
Size: 62 151 1817 865 298 3483
Fragments arranged by size:
3483 1817 865 298 151 62
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5339 882
Size: 617 3840 2219
Fragments arranged by size:
3840 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2417 4467 493
Size: 711 1213 2050 2702
Fragments arranged by size:
2702 2050 1213 711
BsaBI GATnn'nnATC
Cuts at: 6670 6670
Size: 6676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 4483 4865 5500
Size: 53 83 186 936 482 2468 382 635
Cuts at: 5500 275
Size: 1451
Fragments arranged by size:
2468 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5109 5507 5781 5928 6339 5109
Size: 398 274 147 411 5446
Fragments arranged by size:
5446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2874 2904 1178
Size: 30 165 30 1471 30 4950
Fragments arranged by size:
4950 1471 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2895 3326 1186
Size: 1709 431 4536
Fragments arranged by size:
4536 1709 431
BseYI C'CCAG_C
Cuts at: 2396 3745 5879 6375 2396
Size: 1349 2134 496 2697
Fragments arranged by size:
2697 2134 1349 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6103
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4126 4887 5915 1540
Size: 275 186 2125 761 1028 2301
Fragments arranged by size:
2301 2125 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2684 4737 4822 5893 2684
Size: 2053 85 1071 3467
Fragments arranged by size:
3467 2053 1071 85
BsmI GAATG_Cn'
Cuts at: 4187 4280 4187
Size: 93 6583
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2684 3493 3995 4737 4822 5893 1391
Size: 399 894 809 502 742 85 1071 2174
Fragments arranged by size:
2174 1071 894 809 742 502 399 85
BspEI T'CCGG_A
Cuts at: 6339 6339
Size: 6676
BspHI T'CATG_A
Cuts at: 4565 6295 4565
Size: 1730 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2998 4031 844
Size: 2154 1033 3489
Fragments arranged by size:
3489 2154 1033
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 4563 1394
Size: 429 200 2540 3507
Fragments arranged by size:
3507 2540 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 2431 2759 5171 5345 65
Size: 1183 1183 328 2412 174 1396
Fragments arranged by size:
2412 1396 1183 1183 328 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5320 5507 1700
Size: 110 3510 187 2869
Fragments arranged by size:
3510 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6676
BssSI C'ACGA_G
Cuts at: 3172 4736 5748 3172
Size: 1564 1012 4100
Fragments arranged by size:
4100 1564 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6676
BstBI TT'CG_AA
Cuts at: 5480 5480
Size: 6676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6676
BstXI CCAn_nnnn'nTGG
Cuts at: 2126 3745 2126
Size: 1619 5057
BstYI r'GATC_y
Cuts at: 1972 4006 4373 4758 4775 6227 6313 6325 6671
Size: 2034 367 385 17 1452 86 12 346
Cuts at: 6671 1972
Size: 1977
Fragments arranged by size:
2034 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5937
Fragments arranged by size:
5937 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 4192 4986 5006 2137
Size: 2055 794 20 3807
Fragments arranged by size:
3807 2055 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6641
DraI TTT'AAA
Cuts at: 3550 3980 3550
Size: 430 6246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5683 809
Size: 507 4367 1802
Fragments arranged by size:
4367 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 4123 5012
Size: 54 1425 50 275 18 663 1630 889
Cuts at: 5012 6641 8
Size: 1629 43
Fragments arranged by size:
1630 1629 1425 889 663 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4123 1537
Size: 275 2311 4090
Fragments arranged by size:
4090 2311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 3242 4606 1353
Size: 677 1212 1364 3423
Fragments arranged by size:
3423 1364 1212 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5263 5637 5783 387
Size: 1064 138 3674 374 146 1280
Fragments arranged by size:
3674 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2011 2098 2277 2634 2711 2722 2817
Size: 393 379 87 179 357 77 11 95
Cuts at: 2817 3177 3636 3689 4739 5336 6123 6391 1239
Size: 360 459 53 1050 597 787 268 1524
Fragments arranged by size:
1524 1050 787 597 459 393 379 360
357 268 179 95 87 77 53 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3711 3711
Size: 6676
EcoO109I rG'GnC_Cy
Cuts at: 3281 3281
Size: 6676
EcoRI G'AATT_C
Cuts at: 3398 3985 4363 3398
Size: 587 378 5711
Fragments arranged by size:
5711 587 378
FspI TGC'GCA
Cuts at: 5182 5182
Size: 6676
HaeII r_GCGC'y
Cuts at: 1678 5823 1678
Size: 4145 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3035 3067 3754 3786 5317
Size: 32 135 32 1463 32 687 32 1531
Cuts at: 5317 5349 5391 5423 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 1463 687 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2960 3318 4020 4266 669
Size: 988 281 1022 358 702 246 3079
Fragments arranged by size:
3079 1022 988 702 358 281 246
HindIII A'AGCT_T
Cuts at: 748 2163 748
Size: 1415 5261
HpaI GTT'AAC
Cuts at: 3318 4266 3318
Size: 948 5728
KpnI G_GTAC'C
Cuts at: 2126 4001 2126
Size: 1875 4801
MfeI C'AATT_G
Cuts at: 2691 4275 2691
Size: 1584 5092
MluI A'CGCG_T
Cuts at: 6552 6552
Size: 6676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2036 2696 3464 5790 5974 1305
Size: 731 660 768 2326 184 2007
Fragments arranged by size:
2326 2007 768 731 660 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 10
Size: 54 1425 343 663 4191
Fragments arranged by size:
4191 1425 663 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 3833 4634 4993 5152 518
Size: 648 1182 1485 801 359 159 2042
Fragments arranged by size:
2042 1485 1182 801 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4092 4769 5917 6162 1606
Size: 2486 677 1148 245 2120
Fragments arranged by size:
2486 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NheI G'CTAG_C
Cuts at: 1052 3589 1052
Size: 2537 4139
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 2891 3385 4034 5579 1132
Size: 97 1662 494 649 1545 2229
Fragments arranged by size:
2229 1662 1545 649 494 97
PasI CC'CwG_GG
Cuts at: 1348 2596 1348
Size: 1248 5428
PciI A'CATG_T
Cuts at: 2887 5575 2887
Size: 2688 3988
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6676
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 6258 6290 4745
Size: 32 1481 32 5131
Fragments arranged by size:
5131 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3281 3281
Size: 6676
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6676
PsiI TTA'TAA
Cuts at: 2195 2826 4246 2195
Size: 631 1420 4625
Fragments arranged by size:
4625 1420 631
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3205 3237 3205
Size: 32 6644
PstI C_TGCA'G
Cuts at: 830 4028 830
Size: 3198 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 2958 4018 1936
Size: 1022 1060 4594
Fragments arranged by size:
4594 1060 1022
SapI GCTCTTCn'nnn_
Cuts at: 2030 2030
Size: 6676
SbfI CC_TGCA'GG
Cuts at: 4028 4028
Size: 6676
ScaI AGT'ACT
Cuts at: 4924 6636 4924
Size: 1712 4964
SfcI C'TryA_G
Cuts at: 826 1045 4024 5159 5840 6031 826
Size: 219 2979 1135 681 191 1471
Fragments arranged by size:
2979 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6676
SmaI CCC'GGG
Cuts at: 1872 4003 1872
Size: 2131 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2786 2848 2999 4780 5681 5943
Size: 197 926 843 62 151 1781 901 262
Cuts at: 5943 820
Size: 1553
Fragments arranged by size:
1781 1553 926 901 843 262 197 151
62
SnaBI TAC'GTA
Cuts at: 493 2417 4467 493
Size: 1924 2050 2702
Fragments arranged by size:
2702 2050 1924
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6676
SphI G_CATG'C
Cuts at: 1132 4034 1132
Size: 2902 3774
SspI AAT'ATT
Cuts at: 5 52 2340 4600 5
Size: 47 2288 2260 2081
Fragments arranged by size:
2288 2260 2081 47
StuI AGG'CCT
Cuts at: 1517 2075 2846 1517
Size: 558 771 5347
Fragments arranged by size:
5347 771 558
StyI C'CwwG_G
Cuts at: 513 1065 3271 3594 4115 513
Size: 552 2206 323 521 3074
Fragments arranged by size:
3074 2206 552 521 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3737 4703 4720 4873 5058 5503 325
Size: 1255 2157 966 17 153 185 445 1498
Fragments arranged by size:
2157 1498 1255 966 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4922 6634 96
Size: 274 80 33 51 1443 2945 1712 138
Fragments arranged by size:
2945 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6636 6668 6636
Size: 32 6644
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6676
XbaI T'CTAG_A
Cuts at: 2179 4012 2179
Size: 1833 4843
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6676
XmaI C'CCGG_G
Cuts at: 1870 4001 1870
Size: 2131 4545
XmnI GAAnn'nnTTC
Cuts at: 4805 4805
Size: 6676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4484 276
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI