Restriction map for
pFN21AE4549
(Circular) (Six-base) MAPSORT of: pFN21AE4549.seq Check: 7424 from: 1 to: 6658
pFN21AE4549 6658 bp
With 182 enzymes: SgfI *
March 28, 2012 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3277 3277
Size: 6658
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2939 4468 278
Size: 53 83 186 936 1403 1529 2468
Fragments arranged by size:
2468 1529 1403 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2415 4001 1937
Size: 478 1586 4594
Fragments arranged by size:
4594 1586 478
Acc65I G'GTAC_C
Cuts at: 2193 3979 2193
Size: 1786 4872
AclI AA'CG_TT
Cuts at: 1563 3264 4785 5158 1563
Size: 1701 1521 373 3063
Fragments arranged by size:
3063 1701 1521 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3699 4721 6105 6373 1632
Size: 2067 1022 1384 268 1917
Fragments arranged by size:
2067 1917 1384 1022 268
AflII C'TTAA_G
Cuts at: 820 1017 2843 820
Size: 197 1826 4635
Fragments arranged by size:
4635 1826 197
AflIII A'CryG_T
Cuts at: 1203 5557 6534 1203
Size: 4354 977 1327
Fragments arranged by size:
4354 1327 977
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 5387 5387
Size: 6658
AleI CACnn'nnGTG
Cuts at: 1166 2357 2711 1166
Size: 1191 354 5113
Fragments arranged by size:
5113 1191 354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2943 3078 1184
Size: 1759 135 4764
Fragments arranged by size:
4764 1759 135
AlwNI CAG_nnn'CTG
Cuts at: 2347 2865 4401 5973 2347
Size: 518 1536 1572 3032
Fragments arranged by size:
3032 1572 1536 518
ApaI G_GGCC'C
Cuts at: 2487 2487
Size: 6658
ApaLI G'TGCA_C
Cuts at: 4715 5871 4715
Size: 1156 5502
ApoI r'AATT_y
Cuts at: 1493 3766 3967 4193 4345 6454 1493
Size: 2273 201 226 152 2109 1697
Fragments arranged by size:
2273 2109 1697 226 201 152
AseI AT'TA_AT
Cuts at: 160 1032 5212 160
Size: 872 4180 1606
Fragments arranged by size:
4180 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2039 3983 1870
Size: 73 67 29 1944 4545
Fragments arranged by size:
4545 1944 73 67 29
BamHI G'GATC_C
Cuts at: 2979 3658 3988 4355 2979
Size: 679 330 367 5282
Fragments arranged by size:
5282 679 367 330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2193 2848 3134 3540 3979
Size: 325 217 627 406 655 286 406 439
Cuts at: 3979 5434 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 655 627 439 406 406 325
286 217
BanII G_rGCy'C
Cuts at: 1391 2105 2487 3484 3759 3977 1391
Size: 714 382 997 275 218 4072
Fragments arranged by size:
4072 997 714 382 275 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5687
BbvCI CC'TCA_GC
Cuts at: 2366 2366
Size: 6658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2876 2910 3787 3821 4849 4883 1903
Size: 34 939 34 877 34 1028 34 3678
Fragments arranged by size:
3678 1028 939 877 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3918 4550 5760 2598
Size: 1320 632 1210 3496
Fragments arranged by size:
3496 1320 1210 632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 2497 5269
Size: 107 122 71 631 605 361 464 2772
Cuts at: 5269 136
Size: 1525
Fragments arranged by size:
2772 1525 631 605 464 361 122 107
71
BglII A'GATC_T
Cuts at: 2745 6653 2745
Size: 3908 2750
BlpI GC'TnA_GC
Cuts at: 4075 4075
Size: 6658
Bme1580I G_kGCm'C
Cuts at: 1790 2383 2487 3543 4719 5875 1790
Size: 593 104 1056 1176 1156 2573
Fragments arranged by size:
2573 1176 1156 1056 593 104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6658
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2835 2951 3568
Size: 416 53 83 399 24 1418 116 617
Cuts at: 3568 4389 5347 442
Size: 821 958 1753
Fragments arranged by size:
1753 1418 958 821 617 416 399 116
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 2230 3566 5318 1239
Size: 919 72 1336 1752 2579
Fragments arranged by size:
2579 1752 1336 919 72
Bpu10I CC'TnA_GC
Cuts at: 2366 2366
Size: 6658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4783 5648 5946 4783
Size: 865 298 5495
Fragments arranged by size:
5495 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5321 882
Size: 617 3822 2219
Fragments arranged by size:
3822 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3745 4449 493
Size: 711 2541 704 2702
Fragments arranged by size:
2702 2541 711 704
BsaBI GATnn'nnATC
Cuts at: 3401 6652 3401
Size: 3251 3407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2170 2275 2936 4465
Size: 53 83 186 936 637 105 661 1529
Cuts at: 4465 4847 5482 275
Size: 382 635 1451
Fragments arranged by size:
1529 1451 936 661 637 635 382 186
105 83 53
BsaWI w'CCGG_w
Cuts at: 5091 5489 5763 5910 6321 5091
Size: 398 274 147 411 5428
Fragments arranged by size:
5428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2177 2207 1178
Size: 30 165 30 687 30 57 30 5629
Fragments arranged by size:
5629 687 165 57 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 1186
Size: 1012 5646
BseYI C'CCAG_C
Cuts at: 2065 2295 3808 5861 6357 2065
Size: 230 1513 2053 496 2366
Fragments arranged by size:
2366 2053 1513 496 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2913 2939 3876 1163
Size: 573 1177 26 937 3945
Fragments arranged by size:
3945 1177 937 573 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2204 3333 4108 4869 5897 1540
Size: 275 186 203 1129 775 761 1028 2301
Fragments arranged by size:
2301 1129 1028 775 761 275 203 186
BsiHKAI G_wGCw'C
Cuts at: 3759 4719 4804 5875 3759
Size: 960 85 1071 4542
Fragments arranged by size:
4542 1071 960 85
BsmI GAATG_Cn'
Cuts at: 4169 4262 4169
Size: 93 6565
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2383 2487 3484 3543 3759 3977
Size: 399 315 278 104 997 59 216 218
Cuts at: 3977 4719 4804 5875 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 997 742 399 315 278 218
216 104 85 59
BspEI T'CCGG_A
Cuts at: 6321 6321
Size: 6658
BspHI T'CATG_A
Cuts at: 3780 4547 6277 3780
Size: 767 1730 4161
Fragments arranged by size:
4161 1730 767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 3126 3277 4013 844
Size: 1511 771 151 736 3489
Fragments arranged by size:
3489 1511 771 736 151
BsrBI CCG'CTC
Cuts at: 1394 1823 4545 1394
Size: 429 2722 3507
Fragments arranged by size:
3507 2722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5153 5327 65
Size: 1183 3905 174 1396
Fragments arranged by size:
3905 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 5302 5489 1700
Size: 110 386 3106 187 2869
Fragments arranged by size:
3106 2869 386 187 110
BsrGI T'GTAC_A
Cuts at: 96 2664 96
Size: 2568 4090
BssHII G'CGCG_C
Cuts at: 1925 2061 1925
Size: 136 6522
BssSI C'ACGA_G
Cuts at: 4718 5730 4718
Size: 1012 5646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6658
BstBI TT'CG_AA
Cuts at: 5462 5462
Size: 6658
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6658
BstXI CCAn_nnnn'nTGG
Cuts at: 2713 3421 2713
Size: 708 5950
BstYI r'GATC_y
Cuts at: 1972 2745 2979 3658 3825 3988 4355 4740 4757
Size: 773 234 679 167 163 367 385 17
Cuts at: 4757 6209 6295 6307 6653 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 773 679 385 367 346 234
167 163 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 3040 3327 513
Size: 552 939 222 814 287 3844
Fragments arranged by size:
3844 939 814 552 287 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2347 487
Size: 683 56 1121 4798
Fragments arranged by size:
4798 1121 683 56
BtsI GCAGTG_nn'
Cuts at: 2195 2383 4174 4968 4988 2195
Size: 188 1791 794 20 3865
Fragments arranged by size:
3865 1791 794 188 20
ClaI AT'CG_AT
Cuts at: 1554 2688 3216 1554
Size: 1134 528 4996
Fragments arranged by size:
4996 1134 528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3559 3594 529
Size: 35 2995 35 3593
Fragments arranged by size:
3593 2995 35 35
DraI TTT'AAA
Cuts at: 3962 3962
Size: 6658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2699 3790 5665 809
Size: 507 1383 1091 1875 1802
Fragments arranged by size:
1875 1802 1383 1091 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2201 2870 3092
Size: 54 1425 50 275 18 371 669 222
Cuts at: 3092 3348 3567 4105 4994 6623 8
Size: 256 219 538 889 1629 43
Fragments arranged by size:
1629 1425 889 669 538 371 275 256
222 219 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2201 4105 1537
Size: 275 389 1904 4090
Fragments arranged by size:
4090 1904 389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3675 4588 1353
Size: 2322 913 3423
Fragments arranged by size:
3423 2322 913
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5245 5619 5765 387
Size: 1064 138 3656 374 146 1280
Fragments arranged by size:
3656 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3757 3757
Size: 6658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2230 3566 3699 4721 5318 6105
Size: 393 526 72 1336 133 1022 597 787
Cuts at: 6105 6373 1239
Size: 268 1524
Fragments arranged by size:
1524 1336 1022 787 597 526 393 268
133 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2474 2474
Size: 6658
EcoO109I rG'GnC_Cy
Cuts at: 2219 2344 2483 2484 2829 2945 2219
Size: 125 139 1 345 116 5932
Fragments arranged by size:
5932 345 139 125 116 1
EcoRI G'AATT_C
Cuts at: 3967 4345 3967
Size: 378 6280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2617 2804 3844 2617
Size: 187 1040 5431
Fragments arranged by size:
5431 1040 187
FspI TGC'GCA
Cuts at: 2351 5164 2351
Size: 2813 3845
HaeII r_GCGC'y
Cuts at: 1678 2039 3245 5805 1678
Size: 361 1206 2560 2531
Fragments arranged by size:
2560 2531 1206 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 3347 3379 3817
Size: 32 135 32 518 32 1225 32 438
Cuts at: 3817 3849 5299 5331 5373 5405 1373
Size: 32 1450 32 42 32 2626
Fragments arranged by size:
2626 1450 1225 518 438 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 3049 4002 4248 669
Size: 988 281 478 633 953 246 3079
Fragments arranged by size:
3079 988 953 633 478 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6658
HpaI GTT'AAC
Cuts at: 4248 4248
Size: 6658
KpnI G_GTAC'C
Cuts at: 2197 3983 2197
Size: 1786 4872
MfeI C'AATT_G
Cuts at: 4257 4257
Size: 6658
MluI A'CGCG_T
Cuts at: 6534 6534
Size: 6658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2757 3730 5772 5956 1305
Size: 1452 973 2042 184 2007
Fragments arranged by size:
2042 2007 1452 973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3350 3569 10
Size: 54 1425 343 1518 219 3099
Fragments arranged by size:
3099 1518 1425 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2388 2711 3060 3287 4616 4975
Size: 648 1191 31 323 349 227 1329 359
Cuts at: 4975 5134 518
Size: 159 2042
Fragments arranged by size:
2042 1329 1191 648 359 349 323 227
159 31
MspA1I CmG'CkG
Cuts at: 1606 2206 2650 2923 3097 3329 3559 3565 4074
Size: 600 444 273 174 232 230 6 509
Cuts at: 4074 4751 5899 6144 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 600 509 444 273 245
232 230 174 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5167
Fragments arranged by size:
5167 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6658
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 2201 4105 2201
Size: 1904 4754
NspI r_CATG'y
Cuts at: 1132 1229 2236 3286 4016 5561 1132
Size: 97 1007 1050 730 1545 2229
Fragments arranged by size:
2229 1545 1050 1007 730 97
PasI CC'CwG_GG
Cuts at: 1348 2833 1348
Size: 1485 5173
PciI A'CATG_T
Cuts at: 5557 5557
Size: 6658
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2400 1108
Size: 1292 5366
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4759 6240 6272 4727
Size: 32 1481 32 5113
Fragments arranged by size:
5113 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2344 2829 2344
Size: 485 6173
PshAI GACnn'nnGTC
Cuts at: 1613 2274 1613
Size: 661 5997
PsiI TTA'TAA
Cuts at: 4228 4228
Size: 6658
PspOMI G'GGCC_C
Cuts at: 2483 2483
Size: 6658
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 6626
PstI C_TGCA'G
Cuts at: 830 2076 2649 3564 4010 830
Size: 1246 573 915 446 3478
Fragments arranged by size:
3478 1246 915 573 446
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
PvuII CAG'CTG
Cuts at: 2923 3559 3565 2923
Size: 636 6 6016
Fragments arranged by size:
6016 636 6
SacI G_AGCT'C
Cuts at: 3759 3759
Size: 6658
SacII CC_GC'GG
Cuts at: 3330 3330
Size: 6658
SalI G'TCGA_C
Cuts at: 1936 2414 4000 1936
Size: 478 1586 4594
Fragments arranged by size:
4594 1586 478
SanDI GG'GwC_CC
Cuts at: 2829 2829
Size: 6658
SbfI CC_TGCA'GG
Cuts at: 4010 4010
Size: 6658
ScaI AGT'ACT
Cuts at: 4906 6618 4906
Size: 1712 4946
SexAI A'CCwGG_T
Cuts at: 2469 3902 2469
Size: 1433 5225
SfcI C'TryA_G
Cuts at: 826 1045 2072 2645 3560 4006 5141 5822 6013
Size: 219 1027 573 915 446 1135 681 191
Cuts at: 6013 826
Size: 1471
Fragments arranged by size:
1471 1135 1027 915 681 573 446 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 2012 3985 1872
Size: 140 1973 4545
Fragments arranged by size:
4545 1973 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2843 4762 5663 5925 820
Size: 197 926 900 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 4449 493
Size: 3956 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6658
SphI G_CATG'C
Cuts at: 1132 4016 1132
Size: 2884 3774
SspI AAT'ATT
Cuts at: 5 52 4582 5
Size: 47 4530 2081
Fragments arranged by size:
4530 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6658
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 3420 4097 513
Size: 552 939 588 828 677 3074
Fragments arranged by size:
3074 939 828 677 588 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 2392 2689 3587 4685 4702 4855
Size: 1255 707 105 297 898 1098 17 153
Cuts at: 4855 5040 5485 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1098 898 707 445 297 185
153 105 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2656 2664 4904
Size: 274 80 33 51 1443 679 8 2240
Cuts at: 4904 6616 96
Size: 1712 138
Fragments arranged by size:
2240 1712 1443 679 274 138 80 51
33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2362 2394 3401 3433 6618 6650 2362
Size: 32 1007 32 3185 32 2370
Fragments arranged by size:
3185 2370 1007 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6658
XbaI T'CTAG_A
Cuts at: 3994 3994
Size: 6658
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6658
XmaI C'CCGG_G
Cuts at: 1870 2010 3983 1870
Size: 140 1973 4545
Fragments arranged by size:
4545 1973 140
XmnI GAAnn'nnTTC
Cuts at: 3029 3083 3110 4787 3029
Size: 54 27 1677 4900
Fragments arranged by size:
4900 1677 54 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2937 4466 276
Size: 53 83 186 936 1403 1529 2468
Fragments arranged by size:
2468 1529 1403 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI RsrII SapI SfiI SfoI SrfI SwaI
XcmI