Restriction map for
pFN21AE4548
(Circular) (Six-base) MAPSORT of: pFN21AE4548.seq Check: 8676 from: 1 to: 6646
pFN21AE4548 6646 bp
With 182 enzymes: SgfI *
March 28, 2012 14:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 2086 2462 3967 2086
Size: 376 1505 4765
Fragments arranged by size:
4765 1505 376
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 2859 3009 3438 3765 4709 6093 6361
Size: 846 381 150 429 327 944 1384 268
Cuts at: 6361 1632
Size: 1917
Fragments arranged by size:
1917 1384 944 846 429 381 327 268
150
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6449
AflIII A'CryG_T
Cuts at: 1203 5545 6522 1203
Size: 4342 977 1327
Fragments arranged by size:
4342 1327 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6646
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2179 2211 2179
Size: 32 6614
AlwNI CAG_nnn'CTG
Cuts at: 3233 4389 5961 3233
Size: 1156 1572 3918
Fragments arranged by size:
3918 1572 1156
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 3423 3487 3674 3727 3955 4181 4333 6442
Size: 1930 64 187 53 228 226 152 2109
Cuts at: 6442 1493
Size: 1697
Fragments arranged by size:
2109 1930 1697 228 226 187 152 64
53
AseI AT'TA_AT
Cuts at: 160 1032 3311 5200 160
Size: 872 2279 1889 1606
Fragments arranged by size:
2279 1889 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 3971 1870
Size: 73 114 1914 4545
Fragments arranged by size:
4545 1914 114 73
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2321 2462 3241 3967
Size: 325 217 627 299 235 141 779 726
Cuts at: 3967 5422 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 779 726 627 325 299 235
217 141
BanII G_rGCy'C
Cuts at: 1391 2032 3892 3965 1391
Size: 641 1860 73 4072
Fragments arranged by size:
4072 1860 641 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 3253 928
Size: 971 359 995 4321
Fragments arranged by size:
4321 995 971 359
BbvCI CC'TCA_GC
Cuts at: 2554 2554
Size: 6646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4837 4871 1903
Size: 34 2900 34 3678
Fragments arranged by size:
3678 2900 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4538 5748 4538
Size: 1210 5436
BclI T'GATC_A
Cuts at: 1548 3859 1548
Size: 2311 4335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 5257 136
Size: 107 122 71 631 605 376 3209 1525
Fragments arranged by size:
3209 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 6641 6641
Size: 6646
BlpI GC'TnA_GC
Cuts at: 4063 4063
Size: 6646
Bme1580I G_kGCm'C
Cuts at: 1790 2176 2705 4707 5863 1790
Size: 386 529 2002 1156 2573
Fragments arranged by size:
2573 2002 1156 529 386
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2187 3886 4377
Size: 416 53 83 399 24 770 1699 491
Cuts at: 4377 5335 442
Size: 958 1753
Fragments arranged by size:
1753 1699 958 770 491 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3834 1056
Size: 2778 3868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 5306 1239
Size: 1205 2862 2579
Fragments arranged by size:
2862 2579 1205
Bpu10I CC'TnA_GC
Cuts at: 2554 2628 2554
Size: 74 6572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3738 4771 5636 5934 3738
Size: 1033 865 298 4450
Fragments arranged by size:
4450 1033 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5309 882
Size: 617 3810 2219
Fragments arranged by size:
3810 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4437 493
Size: 711 3233 2702
Fragments arranged by size:
3233 2702 711
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4453 4835 5470 275
Size: 53 83 186 936 2920 382 635 1451
Fragments arranged by size:
2920 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5079 5477 5751 5898 6309 5079
Size: 398 274 147 411 5416
Fragments arranged by size:
5416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6421
Fragments arranged by size:
6421 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 1186
Size: 1255 5391
BseYI C'CCAG_C
Cuts at: 2581 3003 3037 5849 6345 2581
Size: 422 34 2812 496 2882
Fragments arranged by size:
2882 2812 496 422 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6073
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2251 4096 4857 5885 1540
Size: 275 186 250 1845 761 1028 2301
Fragments arranged by size:
2301 1845 1028 761 275 250 186
BsiHKAI G_wGCw'C
Cuts at: 4707 4792 5863 4707
Size: 85 1071 5490
Fragments arranged by size:
5490 1071 85
BsmI GAATG_Cn'
Cuts at: 4157 4250 4157
Size: 93 6553
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2176 2705 3892 3965 4707 4792
Size: 399 242 144 529 1187 73 742 85
Cuts at: 4792 5863 1391
Size: 1071 2174
Fragments arranged by size:
2174 1187 1071 742 529 399 242 144
85 73
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2653 4001 844
Size: 1809 1348 3489
Fragments arranged by size:
3489 1809 1348
BsrBI CCG'CTC
Cuts at: 1394 1823 2132 4533 1394
Size: 429 309 2401 3507
Fragments arranged by size:
3507 2401 429 309
BsrDI GCAATG_nn'
Cuts at: 65 1248 2288 3612 5141 5315 65
Size: 1183 1040 1324 1529 174 1396
Fragments arranged by size:
1529 1396 1324 1183 1040 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5290 5477 1700
Size: 110 3480 187 2869
Fragments arranged by size:
3480 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 2100 4706 5718 2100
Size: 2606 1012 3028
Fragments arranged by size:
3028 2606 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3776 794
Size: 2982 3664
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 2369 1184
Size: 1185 5461
BstXI CCAn_nnnn'nTGG
Cuts at: 2698 2698
Size: 6646
BstYI r'GATC_y
Cuts at: 1972 3976 4343 4728 4745 6197 6283 6295 6641
Size: 2004 367 385 17 1452 86 12 346
Cuts at: 6641 1972
Size: 1977
Fragments arranged by size:
2004 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2514 2514
Size: 6646
BtgI C'CryG_G
Cuts at: 513 1065 2045 2210 2697 513
Size: 552 980 165 487 4462
Fragments arranged by size:
4462 980 552 487 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5907
Fragments arranged by size:
5907 683 56
BtsI GCAGTG_nn'
Cuts at: 2601 4162 4956 4976 2601
Size: 1561 794 20 4271
Fragments arranged by size:
4271 1561 794 20
ClaI AT'CG_AT
Cuts at: 1554 3354 1554
Size: 1800 4846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6611
DraI TTT'AAA
Cuts at: 2533 2946 3471 3950 2533
Size: 413 525 479 5229
Fragments arranged by size:
5229 525 479 413
DraIII CAC_nnn'GTG
Cuts at: 3868 3868
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2982 4093
Size: 54 1425 50 275 18 418 734 1111
Cuts at: 4093 4982 6611 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1111 889 734 418 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2248 4093 1537
Size: 275 436 1845 4090
Fragments arranged by size:
4090 1845 436 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4576 1353
Size: 3223 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5233 5607 5753 387
Size: 1064 138 3644 374 146 1280
Fragments arranged by size:
3644 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 2478 2859 3009 3438 3765 4709
Size: 393 812 34 381 150 429 327 944
Cuts at: 4709 5306 6093 6361 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 944 812 787 597 429 393 381
327 268 150 34
EcoNI CCTnn'n_nnAGG
Cuts at: 3225 3225
Size: 6646
EcoO109I rG'GnC_Cy
Cuts at: 3230 3230
Size: 6646
EcoRI G'AATT_C
Cuts at: 3674 3955 4333 3674
Size: 281 378 5987
Fragments arranged by size:
5987 378 281
EcoRV GAT'ATC
Cuts at: 3169 3271 3169
Size: 102 6544
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 5793 1678
Size: 4115 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2475 2507 3158 3190 5287
Size: 32 135 32 903 32 651 32 2097
Cuts at: 5287 5319 5361 5393 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2097 903 651 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3990 4236 669
Size: 988 281 2052 246 3079
Fragments arranged by size:
3079 2052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3450 748
Size: 2702 3944
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 2090 2466 3971 2090
Size: 376 1505 4765
Fragments arranged by size:
4765 1505 376
MfeI C'AATT_G
Cuts at: 3771 4245 3771
Size: 474 6172
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 3020 3865 5760 5944 1305
Size: 1084 631 845 1895 184 2007
Fragments arranged by size:
2007 1895 1084 845 631 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4824
Fragments arranged by size:
4824 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 4604 4963 5122 518
Size: 648 1530 1908 359 159 2042
Fragments arranged by size:
2042 1908 1530 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2987 3212 4062 4739 5887 6132 1606
Size: 606 775 225 850 677 1148 245 2120
Fragments arranged by size:
2120 1148 850 775 677 606 245 225
NcoI C'CATG_G
Cuts at: 513 1065 2045 2697 513
Size: 552 980 652 4462
Fragments arranged by size:
4462 980 652 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NheI G'CTAG_C
Cuts at: 1052 3830 1052
Size: 2778 3868
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NruI TCG'CGA
Cuts at: 2113 2113
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 2662 3468 3762 4004 5549 1132
Size: 97 1433 806 294 242 1545 2229
Fragments arranged by size:
2229 1545 1433 806 294 242 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6646
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6646
PfoI T'CCnGG_A
Cuts at: 3232 3232
Size: 6646
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3230 3230
Size: 6646
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 3512 4216 3512
Size: 704 5942
PspXI vC'TCGA_Gb
Cuts at: 2057 2057
Size: 6646
PstI C_TGCA'G
Cuts at: 830 2232 3562 3805 3998 830
Size: 1402 1330 243 193 3478
Fragments arranged by size:
3478 1402 1330 243 193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
PvuII CAG'CTG
Cuts at: 3212 3212
Size: 6646
SacII CC_GC'GG
Cuts at: 2213 2213
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SbfI CC_TGCA'GG
Cuts at: 3562 3998 3562
Size: 436 6210
ScaI AGT'ACT
Cuts at: 3553 4894 6606 3553
Size: 1341 1712 3593
Fragments arranged by size:
3593 1712 1341
SfcI C'TryA_G
Cuts at: 826 1045 2151 2228 2787 2920 3252 3558 3662
Size: 219 1106 77 559 133 332 306 104
Cuts at: 3662 3801 3994 5129 5810 6001 826
Size: 139 193 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 681 559 332 306 219
193 191 139 133 104 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 3753 4750 5651 5913 820
Size: 197 926 114 1696 997 901 262 1553
Fragments arranged by size:
1696 1553 997 926 901 262 197 114
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 4004 1132
Size: 2872 3774
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 2528 1517
Size: 1011 5635
StyI C'CwwG_G
Cuts at: 513 1065 2045 2697 4085 513
Size: 552 980 652 1388 3074
Fragments arranged by size:
3074 1388 980 652 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2720 4673 4690 4843 5028 5473 325
Size: 1255 1140 1953 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 1140 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2800 3551 4892
Size: 274 80 33 51 1443 823 751 1341
Cuts at: 4892 6604 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1341 823 751 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3660 3692 6606 6638 3660
Size: 32 2914 32 3668
Fragments arranged by size:
3668 2914 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2695 2695
Size: 6646
XhoI C'TCGA_G
Cuts at: 1943 2057 1943
Size: 114 6532
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI
PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI