Restriction map for
pFN21AE4547
(Circular) (Six-base) MAPSORT of: pFN21AE4547.seq Check: 6755 from: 1 to: 6634
pFN21AE4547 6634 bp
With 182 enzymes: SgfI *
March 28, 2012 14:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3218 3218
Size: 6634
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3012 4444 278
Size: 53 83 186 936 1476 1432 2468
Fragments arranged by size:
2468 1476 1432 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3977 1937
Size: 2040 4594
Acc65I G'GTAC_C
Cuts at: 3955 3955
Size: 6634
AclI AA'CG_TT
Cuts at: 1563 4761 5134 1563
Size: 3198 373 3063
Fragments arranged by size:
3198 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4697 6081 6349 1632
Size: 3065 1384 268 1917
Fragments arranged by size:
3065 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6437
AflIII A'CryG_T
Cuts at: 1203 2514 2892 5533 6510 1203
Size: 1311 378 2641 977 1327
Fragments arranged by size:
2641 1327 1311 977 378
AgeI A'CCGG_T
Cuts at: 5465 5465
Size: 6634
AhdI GACnn_n'nnGTC
Cuts at: 5363 5363
Size: 6634
AleI CACnn'nnGTG
Cuts at: 1166 2255 1166
Size: 1089 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6634
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2333 2365 2333
Size: 32 6602
AlwNI CAG_nnn'CTG
Cuts at: 3482 4377 5949 3482
Size: 895 1572 4167
Fragments arranged by size:
4167 1572 895
ApaLI G'TGCA_C
Cuts at: 4691 5847 4691
Size: 1156 5478
ApoI r'AATT_y
Cuts at: 1493 2741 3943 4169 4321 6430 1493
Size: 1248 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1248 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5188 160
Size: 872 4156 1606
Fragments arranged by size:
4156 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6634
AvaI C'yCGr_G
Cuts at: 1870 1943 2357 3959 1870
Size: 73 414 1602 4545
Fragments arranged by size:
4545 1602 414 73
BamHI G'GATC_C
Cuts at: 3964 4331 3964
Size: 367 6267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2618 3955 5410 618
Size: 325 217 627 258 573 1337 1455 1842
Fragments arranged by size:
1842 1455 1337 627 573 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2025 2511 2538 3029 3953 1391
Size: 634 486 27 491 924 4072
Fragments arranged by size:
4072 924 634 491 486 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3316 3640 928
Size: 971 1417 324 3922
Fragments arranged by size:
3922 1417 971 324
BbvCI CC'TCA_GC
Cuts at: 3591 3591
Size: 6634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3800 3834 4825 4859 1903
Size: 34 50 34 1779 34 991 34 3678
Fragments arranged by size:
3678 1779 991 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4526 5736 4526
Size: 1210 5424
BclI T'GATC_A
Cuts at: 1548 2439 1548
Size: 891 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3125 5245
Size: 107 122 71 631 605 337 1116 2120
Cuts at: 5245 136
Size: 1525
Fragments arranged by size:
2120 1525 1116 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2364 6629 2364
Size: 4265 2369
BlpI GC'TnA_GC
Cuts at: 2381 3539 4051 2381
Size: 1158 512 4964
Fragments arranged by size:
4964 1158 512
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2451 2621 2788 2911 4695 5851 1790
Size: 258 403 170 167 123 1784 1156 2573
Fragments arranged by size:
2573 1784 1156 403 258 170 167 123
BmgBI CAC'GTC
Cuts at: 1318 2895 1318
Size: 1577 5057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4365 5323 442
Size: 416 53 83 399 24 2948 958 1753
Fragments arranged by size:
2948 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6634
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2881 2383
Size: 498 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2700 2821 2876 2972 3239 3656 3773 3817
Size: 1461 121 55 96 267 417 117 44
Cuts at: 3817 3827 5294 1239
Size: 10 1467 2579
Fragments arranged by size:
2579 1467 1461 417 267 121 117 96
55 44 10
Bpu10I CC'TnA_GC
Cuts at: 2768 3467 3591 2768
Size: 699 124 5811
Fragments arranged by size:
5811 699 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3867 4759 5624 5922 3867
Size: 892 865 298 4579
Fragments arranged by size:
4579 892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5297 882
Size: 617 3798 2219
Fragments arranged by size:
3798 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2134 3416 4425 493
Size: 711 930 1282 1009 2702
Fragments arranged by size:
2702 1282 1009 930 711
BsaBI GATnn'nnATC
Cuts at: 3515 6628 3515
Size: 3113 3521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3009 4441 4823 5458
Size: 53 83 186 936 1476 1432 382 635
Cuts at: 5458 275
Size: 1451
Fragments arranged by size:
1476 1451 1432 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5067 5465 5739 5886 6297 5067
Size: 398 274 147 411 5404
Fragments arranged by size:
5404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2354 2384 3481 3511 1178
Size: 30 165 30 951 30 1097 30 4301
Fragments arranged by size:
4301 1097 951 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2712 3563 3794 3938 1186
Size: 1189 337 851 231 144 3882
Fragments arranged by size:
3882 1189 851 337 231 144
BseYI C'CCAG_C
Cuts at: 2126 3376 3902 5837 6333 2126
Size: 1250 526 1935 496 2427
Fragments arranged by size:
2427 1935 1250 526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 3068 3155 3194 1163
Size: 573 543 789 87 39 4603
Fragments arranged by size:
4603 789 573 543 87 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2403 3144 4084 4845 5873 1540
Size: 275 186 402 741 940 761 1028 2301
Fragments arranged by size:
2301 1028 940 761 741 402 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2598 2930 3494 3759 4695 4780 5851 2025
Size: 573 332 564 265 936 85 1071 2808
Fragments arranged by size:
2808 1071 936 573 564 332 265 85
BsmI GAATG_Cn'
Cuts at: 4145 4238 4145
Size: 93 6541
BsmBI CGTCTCn'nnnn_
Cuts at: 2390 2390
Size: 6634
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2048 2451 2511 2538 2598 2621
Size: 399 235 23 403 60 27 60 23
Cuts at: 2621 2788 2911 2930 3029 3494 3759 3953 4695
Size: 167 123 19 99 465 265 194 742
Cuts at: 4695 4780 5851 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 465 403 399 265 235
194 167 123 99 85 60 60 27
23 23 19
BspEI T'CCGG_A
Cuts at: 6297 6297
Size: 6634
BspHI T'CATG_A
Cuts at: 3004 3568 4523 6253 3004
Size: 564 955 1730 3385
Fragments arranged by size:
3385 1730 955 564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3218 3381 3989 844
Size: 2374 163 608 3489
Fragments arranged by size:
3489 2374 608 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 2772 4521 1394
Size: 429 332 617 1749 3507
Fragments arranged by size:
3507 1749 617 429 332
BsrDI GCAATG_nn'
Cuts at: 65 1248 5129 5303 65
Size: 1183 3881 174 1396
Fragments arranged by size:
3881 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2568 2715 2956 3534 3908 5278 5465
Size: 110 758 147 241 578 374 1370 187
Cuts at: 5465 1700
Size: 2869
Fragments arranged by size:
2869 1370 758 578 374 241 187 147
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6634
BssHII G'CGCG_C
Cuts at: 1925 2815 1925
Size: 890 5744
BssSI C'ACGA_G
Cuts at: 2056 2720 2904 4694 5706 2056
Size: 664 184 1790 1012 2984
Fragments arranged by size:
2984 1790 1012 664 184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3055 794
Size: 2261 4373
BstBI TT'CG_AA
Cuts at: 5438 5438
Size: 6634
BstEII G'GTnAC_C
Cuts at: 1184 3436 1184
Size: 2252 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 2134
Size: 6634
BstYI r'GATC_y
Cuts at: 1972 2091 2364 3178 3964 4331 4716 4733 6185
Size: 119 273 814 786 367 385 17 1452
Cuts at: 6185 6271 6283 6629 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 814 786 385 367 346 273
119 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3683 3683
Size: 6634
BtgI C'CryG_G
Cuts at: 513 1065 2015 2253 2332 2373 2538 513
Size: 552 950 238 79 41 165 4609
Fragments arranged by size:
4609 950 552 238 165 79 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2485 2536 487
Size: 683 56 1259 51 4585
Fragments arranged by size:
4585 1259 683 56 51
BtsI GCAGTG_nn'
Cuts at: 2442 2673 4150 4944 4964 2442
Size: 231 1477 794 20 4112
Fragments arranged by size:
4112 1477 794 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6634
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6599
DraI TTT'AAA
Cuts at: 3938 3938
Size: 6634
DraIII CAC_nnn'GTG
Cuts at: 2437 2437
Size: 6634
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2654 5641 809
Size: 507 1338 2987 1802
Fragments arranged by size:
2987 1802 1338 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2172 2400
Size: 54 1425 50 275 18 267 75 228
Cuts at: 2400 3141 4081 4970 6599 8
Size: 741 940 889 1629 43
Fragments arranged by size:
1629 1425 940 889 741 275 267 228
75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2400 3141 4081 1537
Size: 275 588 741 940 4090
Fragments arranged by size:
4090 940 741 588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2942 3696 3791 4564 1353
Size: 1589 754 95 773 3423
Fragments arranged by size:
3423 1589 773 754 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2764 5221 5595 5741 387
Size: 1064 138 1175 2457 374 146 1280
Fragments arranged by size:
2457 1280 1175 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2023
Size: 6634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2700 2821 2876 2972 3239 3656 3773
Size: 393 1068 121 55 96 267 417 117
Cuts at: 3773 3817 3827 4697 5294 6081 6349 1239
Size: 44 10 870 597 787 268 1524
Fragments arranged by size:
1524 1068 870 787 597 417 393 268
267 121 117 96 55 44 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2873 2873
Size: 6634
EcoO109I rG'GnC_Cy
Cuts at: 2011 2764 2968 3848 3898 2011
Size: 753 204 880 50 4747
Fragments arranged by size:
4747 880 753 204 50
EcoRI G'AATT_C
Cuts at: 2741 3943 4321 2741
Size: 1202 378 5054
Fragments arranged by size:
5054 1202 378
FspI TGC'GCA
Cuts at: 2792 2801 5140 2792
Size: 9 2339 4286
Fragments arranged by size:
4286 2339 9
HaeII r_GCGC'y
Cuts at: 1678 2476 5781 1678
Size: 798 3305 2531
Fragments arranged by size:
3305 2531 798
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 5275 5307 5349
Size: 32 135 32 782 32 2889 32 42
Cuts at: 5349 5381 1373
Size: 32 2626
Fragments arranged by size:
2889 2626 782 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3978 4224 669
Size: 988 281 2040 246 3079
Fragments arranged by size:
3079 2040 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6634
HpaI GTT'AAC
Cuts at: 4224 4224
Size: 6634
KpnI G_GTAC'C
Cuts at: 3959 3959
Size: 6634
MfeI C'AATT_G
Cuts at: 4233 4233
Size: 6634
MluI A'CGCG_T
Cuts at: 6510 6510
Size: 6634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 5748 5932 1305
Size: 939 3504 184 2007
Fragments arranged by size:
3504 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 10
Size: 54 1425 343 267 4545
Fragments arranged by size:
4545 1425 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 3332 4592 4951 5110 518
Size: 648 1089 1077 1260 359 159 2042
Fragments arranged by size:
2042 1260 1089 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2334 2546 3766 4050 4727 5875 6120 1606
Size: 728 212 1220 284 677 1148 245 2120
Fragments arranged by size:
2120 1220 1148 728 677 284 245 212
NaeI GCC'GGC
Cuts at: 3536 3536
Size: 6634
NcoI C'CATG_G
Cuts at: 513 1065 2373 513
Size: 552 1308 4774
Fragments arranged by size:
4774 1308 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6634
NgoMIV G'CCGG_C
Cuts at: 3534 3534
Size: 6634
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6634
NotI GC'GGCC_GC
Cuts at: 4081 4081
Size: 6634
NspI r_CATG'y
Cuts at: 1132 1229 3992 5537 1132
Size: 97 2763 1545 2229
Fragments arranged by size:
2763 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3860 1348
Size: 2512 4122
PciI A'CATG_T
Cuts at: 5533 5533
Size: 6634
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 5008
PmeI GTTT'AAAC
Cuts at: 3938 3938
Size: 6634
PmlI CAC'GTG
Cuts at: 3416 3416
Size: 6634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4703 4735 6216 6248 4703
Size: 32 1481 32 5089
Fragments arranged by size:
5089 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2764 3898 2764
Size: 1134 5500
PshAI GACnn'nnGTC
Cuts at: 1613 3729 1613
Size: 2116 4518
PsiI TTA'TAA
Cuts at: 4204 4204
Size: 6634
PstI C_TGCA'G
Cuts at: 830 3063 3986 830
Size: 2233 923 3478
Fragments arranged by size:
3478 2233 923
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6634
PvuII CAG'CTG
Cuts at: 2546 3766 2546
Size: 1220 5414
SacI G_AGCT'C
Cuts at: 2025 2025
Size: 6634
SacII CC_GC'GG
Cuts at: 2335 2335
Size: 6634
SalI G'TCGA_C
Cuts at: 1936 3976 1936
Size: 2040 4594
SanDI GG'GwC_CC
Cuts at: 3898 3898
Size: 6634
SbfI CC_TGCA'GG
Cuts at: 3986 3986
Size: 6634
ScaI AGT'ACT
Cuts at: 4882 6594 4882
Size: 1712 4922
SfcI C'TryA_G
Cuts at: 826 1045 3059 3982 5117 5798 5989 826
Size: 219 2014 923 1135 681 191 1471
Fragments arranged by size:
2014 1471 1135 923 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6634
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6634
SmaI CCC'GGG
Cuts at: 1872 3961 1872
Size: 2089 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3882 4738 5639 5901 820
Size: 197 926 1939 856 901 262 1553
Fragments arranged by size:
1939 1553 926 901 856 262 197
SnaBI TAC'GTA
Cuts at: 493 4425 493
Size: 3932 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6634
SphI G_CATG'C
Cuts at: 1132 3992 1132
Size: 2860 3774
SspI AAT'ATT
Cuts at: 5 52 4558 5
Size: 47 4506 2081
Fragments arranged by size:
4506 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6634
StyI C'CwwG_G
Cuts at: 513 1065 2313 2373 2484 3042 3360 4073 513
Size: 552 1248 60 111 558 318 713 3074
Fragments arranged by size:
3074 1248 713 558 552 318 111 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 2858 4661 4678 4831 5016 5461
Size: 1255 1020 258 1803 17 153 185 445
Cuts at: 5461 325
Size: 1498
Fragments arranged by size:
1803 1498 1255 1020 445 258 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3582 4880 6592
Size: 274 80 33 51 1443 1605 1298 1712
Cuts at: 6592 96
Size: 138
Fragments arranged by size:
1712 1605 1443 1298 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2767 2799 3405 3437 6594 6626 2767
Size: 32 606 32 3157 32 2775
Fragments arranged by size:
3157 2775 606 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3011 1316
Size: 1695 4939
XbaI T'CTAG_A
Cuts at: 3970 3970
Size: 6634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3631 3631
Size: 6634
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6634
XmaI C'CCGG_G
Cuts at: 1870 3959 1870
Size: 2089 4545
XmnI GAAnn'nnTTC
Cuts at: 2729 4763 2729
Size: 2034 4600
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3010 4442 276
Size: 53 83 186 936 1476 1432 2468
Fragments arranged by size:
2468 1476 1432 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI