Restriction map for
pFN21AE4546
(Circular) (Six-base) MAPSORT of: pFN21AE4546.seq Check: 4053 from: 1 to: 6625
pFN21AE4546 6625 bp
With 182 enzymes: SgfI *
March 28, 2012 14:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2257 2257
Size: 6625
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2131 4435 278
Size: 53 83 186 936 595 2304 2468
Fragments arranged by size:
2468 2304 936 595 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 3453 3968 1937
Size: 1038 478 515 4594
Fragments arranged by size:
4594 1038 515 478
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 3924 4752 5125 1563
Size: 2361 828 373 3063
Fragments arranged by size:
3063 2361 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3759 4688 6072 6340 1632
Size: 2127 929 1384 268 1917
Fragments arranged by size:
2127 1917 1384 929 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 3795 5524 6501 1203
Size: 2592 1729 977 1327
Fragments arranged by size:
2592 1729 1327 977
AgeI A'CCGG_T
Cuts at: 3507 5456 3507
Size: 1949 4676
AhdI GACnn_n'nnGTC
Cuts at: 2395 5354 2395
Size: 2959 3666
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6625
AlwNI CAG_nnn'CTG
Cuts at: 2095 4368 5940 2095
Size: 2273 1572 2780
Fragments arranged by size:
2780 2273 1572
ApaLI G'TGCA_C
Cuts at: 4682 5838 4682
Size: 1156 5469
ApoI r'AATT_y
Cuts at: 1493 2297 2422 2826 3756 3934 4160 4312 6421
Size: 804 125 404 930 178 226 152 2109
Cuts at: 6421 1493
Size: 1697
Fragments arranged by size:
2109 1697 930 804 404 226 178 152
125
AseI AT'TA_AT
Cuts at: 160 1032 3689 5179 160
Size: 872 2657 1490 1606
Fragments arranged by size:
2657 1606 1490 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 3950 1870
Size: 73 2007 4545
Fragments arranged by size:
4545 2007 73
BamHI G'GATC_C
Cuts at: 2352 3661 3955 4322 2352
Size: 1309 294 367 4655
Fragments arranged by size:
4655 1309 367 294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2209 3946 5401 618
Size: 325 217 627 422 1737 1455 1842
Fragments arranged by size:
1842 1737 1455 627 422 325 217
BanII G_rGCy'C
Cuts at: 1391 3944 1391
Size: 2553 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3306 3313 928
Size: 971 1407 7 4240
Fragments arranged by size:
4240 1407 971 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 2692 3348 1548
Size: 1144 656 4825
Fragments arranged by size:
4825 1144 656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 5236 136
Size: 107 122 71 631 605 535 3029 1525
Fragments arranged by size:
3029 1525 631 605 535 122 107 71
BglII A'GATC_T
Cuts at: 6620 6620
Size: 6625
BlpI GC'TnA_GC
Cuts at: 2096 2184 4042 2096
Size: 88 1858 4679
Fragments arranged by size:
4679 1858 88
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2898 4686 5842 1790
Size: 258 850 1788 1156 2573
Fragments arranged by size:
2573 1788 1156 850 258
BmgBI CAC'GTC
Cuts at: 1318 2049 1318
Size: 731 5894
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 3562 3824
Size: 416 53 83 399 24 2042 103 262
Cuts at: 3824 4356 5314 442
Size: 532 958 1753
Fragments arranged by size:
2042 1753 958 532 416 399 262 103
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2468 1056
Size: 1412 5213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2174 2174
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 3114 3615 5285 1239
Size: 905 970 501 1670 2579
Fragments arranged by size:
2579 1670 970 905 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2684 4750 5615 5913 2684
Size: 2066 865 298 3396
Fragments arranged by size:
3396 2066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2145 5288 882
Size: 617 646 3143 2219
Fragments arranged by size:
3143 2219 646 617
BsaAI yAC'GTr
Cuts at: 493 1204 3101 4416 493
Size: 711 1897 1315 2702
Fragments arranged by size:
2702 1897 1315 711
BsaBI GATnn'nnATC
Cuts at: 3045 3353 6619 3045
Size: 308 3266 3051
Fragments arranged by size:
3266 3051 308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2128 4432 4814 5449
Size: 53 83 186 936 595 2304 382 635
Cuts at: 5449 275
Size: 1451
Fragments arranged by size:
2304 1451 936 635 595 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3507 5058 5456 5730 5877 6288 3507
Size: 1551 398 274 147 411 3844
Fragments arranged by size:
3844 1551 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6400
Fragments arranged by size:
6400 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6625
BseYI C'CCAG_C
Cuts at: 5828 6324 5828
Size: 496 6129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3692 1163
Size: 573 1956 4096
Fragments arranged by size:
4096 1956 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3872 4686 4771 5842 3872
Size: 814 85 1071 4655
Fragments arranged by size:
4655 1071 814 85
BsmI GAATG_Cn'
Cuts at: 4136 4229 4136
Size: 93 6532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2898 3872 3944 4686 4771 5842
Size: 399 258 850 974 72 742 85 1071
Cuts at: 5842 1391
Size: 2174
Fragments arranged by size:
2174 1071 974 850 742 399 258 85
72
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 4514 6244 4514
Size: 1730 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2257 3980 844
Size: 1413 1723 3489
Fragments arranged by size:
3489 1723 1413
BsrBI CCG'CTC
Cuts at: 1394 1823 4512 1394
Size: 429 2689 3507
Fragments arranged by size:
3507 2689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5120 5294 65
Size: 1183 3872 174 1396
Fragments arranged by size:
3872 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2154 3507 5269 5456 1700
Size: 110 344 1353 1762 187 2869
Fragments arranged by size:
2869 1762 1353 344 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 4685 5697 4685
Size: 1012 5613
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 794
Size: 1235 5390
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 2483 3503 1184
Size: 1299 1020 4306
Fragments arranged by size:
4306 1299 1020
BstXI CCAn_nnnn'nTGG
Cuts at: 2905 2905
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 2352 2860 3661 3955 4322 4707 4724 6176
Size: 380 508 801 294 367 385 17 1452
Cuts at: 6176 6262 6274 6620 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 801 508 385 380 367 346
294 86 17 12
BstZ17I GTA'TAC
Cuts at: 2976 2976
Size: 6625
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 4141 4935 4955 4141
Size: 794 20 5811
Fragments arranged by size:
5811 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 3805 3929 3805
Size: 124 6501
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2726 3449 5632 809
Size: 507 1410 723 2183 1802
Fragments arranged by size:
2183 1802 1410 723 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2227 4072
Size: 54 1425 50 275 18 369 28 1845
Cuts at: 4072 4961 6590 8
Size: 889 1629 43
Fragments arranged by size:
1845 1629 1425 889 369 275 54 50
43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 4555 1353
Size: 1314 1888 3423
Fragments arranged by size:
3423 1888 1314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2163 5212 5586 5732 387
Size: 1064 138 574 3049 374 146 1280
Fragments arranged by size:
3049 1280 1064 574 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 3114 3615 3759 4688 5285 6072
Size: 393 512 970 501 144 929 597 787
Cuts at: 6072 6340 1239
Size: 268 1524
Fragments arranged by size:
1524 970 929 787 597 512 501 393
268 144
EcoO109I rG'GnC_Cy
Cuts at: 2728 2728
Size: 6625
EcoRI G'AATT_C
Cuts at: 3934 4312 3934
Size: 378 6247
FspI TGC'GCA
Cuts at: 2268 5131 2268
Size: 2863 3762
HaeII r_GCGC'y
Cuts at: 1678 3626 5772 1678
Size: 1948 2146 2531
Fragments arranged by size:
2531 2146 1948
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 3856 3888 5266
Size: 32 135 32 809 32 1443 32 1378
Cuts at: 5266 5298 5340 5372 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 1378 809 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2334 2557 3969 4215 669
Size: 988 281 396 223 1412 246 3079
Fragments arranged by size:
3079 1412 988 396 281 246 223
HindIII A'AGCT_T
Cuts at: 748 3771 748
Size: 3023 3602
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 2118 4224 2118
Size: 2106 4519
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2156 2762 2946 5739 5923 1305
Size: 851 606 184 2793 184 2007
Fragments arranged by size:
2793 2007 851 606 184 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2229 10
Size: 54 1425 343 369 28 4406
Fragments arranged by size:
4406 1425 369 343 54 28
MslI CAynn'nnrTG
Cuts at: 518 1166 4583 4942 5101 518
Size: 648 3417 359 159 2042
Fragments arranged by size:
3417 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2173 2461 4041 4718 5866 6111 1606
Size: 567 288 1580 677 1148 245 2120
Fragments arranged by size:
2120 1580 1148 677 567 288 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 2464 1052
Size: 1412 5213
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2273 2398 2770 3799 3983 5528 1132
Size: 97 1044 125 372 1029 184 1545 2229
Fragments arranged by size:
2229 1545 1044 1029 372 184 125 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 3795 5524 3795
Size: 1729 4896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2138 1108
Size: 1030 5595
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 2728
Size: 6625
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 3156 4195 3156
Size: 1039 5586
PstI C_TGCA'G
Cuts at: 830 3977 830
Size: 3147 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 2173 2173
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 4873 6585 4873
Size: 1712 4913
SfcI C'TryA_G
Cuts at: 826 1045 2101 3435 3973 5108 5789 5980 826
Size: 219 1056 1334 538 1135 681 191 1471
Fragments arranged by size:
1471 1334 1135 1056 681 538 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 3952 1872
Size: 2080 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2699 4729 5630 5892 820
Size: 197 926 756 2030 901 262 1553
Fragments arranged by size:
2030 1553 926 901 756 262 197
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 3169 152
Size: 3017 3608
SphI G_CATG'C
Cuts at: 1132 3983 1132
Size: 2851 3774
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6625
StyI C'CwwG_G
Cuts at: 513 1065 2004 2593 3016 3403 3843 4064 513
Size: 552 939 589 423 387 440 221 3074
Fragments arranged by size:
3074 939 589 552 440 423 387 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4652 4669 4822 5007 5452 325
Size: 1255 3072 17 153 185 445 1498
Fragments arranged by size:
3072 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3277 4871 6583
Size: 274 80 33 51 1443 1300 1594 1712
Cuts at: 6583 96
Size: 138
Fragments arranged by size:
1712 1594 1443 1300 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2997 3029 6585 6617 2997
Size: 32 3556 32 3005
Fragments arranged by size:
3556 3005 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6625
XbaI T'CTAG_A
Cuts at: 2380 3961 2380
Size: 1581 5044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2905 2905
Size: 6625
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 3950 1870
Size: 2080 4545
XmnI GAAnn'nnTTC
Cuts at: 2322 4754 2322
Size: 2432 4193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2129 4433 276
Size: 53 83 186 936 595 2304 2468
Fragments arranged by size:
2468 2304 936 595 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI