Restriction map for
pFN21AE4544
(Circular) (Six-base) MAPSORT of: pFN21AE4544.seq Check: 2142 from: 1 to: 6607
pFN21AE4544 6607 bp
With 182 enzymes: SgfI *
March 28, 2012 14:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2670 3358 3950 1937
Size: 733 688 592 4594
Fragments arranged by size:
4594 733 688 592
Acc65I G'GTAC_C
Cuts at: 2807 3928 2807
Size: 1121 5486
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2723 3725 4670 6054 6322 1632
Size: 644 447 1002 945 1384 268 1917
Fragments arranged by size:
1917 1384 1002 945 644 447 268
AflII C'TTAA_G
Cuts at: 820 1017 2054 820
Size: 197 1037 5373
Fragments arranged by size:
5373 1037 197
AflIII A'CryG_T
Cuts at: 1203 2413 5506 6483 1203
Size: 1210 3093 977 1327
Fragments arranged by size:
3093 1327 1210 977
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2223 2255 2223
Size: 32 6575
AlwNI CAG_nnn'CTG
Cuts at: 2936 4350 5922 2936
Size: 1414 1572 3621
Fragments arranged by size:
3621 1572 1414
ApaLI G'TGCA_C
Cuts at: 4664 5820 4664
Size: 1156 5451
ApoI r'AATT_y
Cuts at: 1493 2758 2890 3390 3550 3695 3861 3916 4142
Size: 1265 132 500 160 145 166 55 226
Cuts at: 4142 4294 6403 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1265 500 226 166 160 152
145 132 55
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 3932 1870
Size: 73 1989 4545
Fragments arranged by size:
4545 1989 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2055 2088 2055
Size: 33 6574
BamHI G'GATC_C
Cuts at: 3937 4304 3937
Size: 367 6240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 2807 3928 5383 618
Size: 325 217 627 774 246 1121 1455 1842
Fragments arranged by size:
1842 1455 1121 774 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2341 3926 1391
Size: 950 1585 4072
Fragments arranged by size:
4072 1585 950
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3127 3453 928
Size: 971 1228 326 4082
Fragments arranged by size:
4082 1228 971 326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4798 4832 1903
Size: 34 2861 34 3678
Fragments arranged by size:
3678 2861 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4499 5709 4499
Size: 1210 5397
BclI T'GATC_A
Cuts at: 1548 3759 1548
Size: 2211 4396
BfrBI ATG'CAT
Cuts at: 3800 3800
Size: 6607
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5218 136
Size: 107 122 71 631 605 3546 1525
Fragments arranged by size:
3546 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2696 3434 6602 2696
Size: 738 3168 2701
Fragments arranged by size:
3168 2701 738
BlpI GC'TnA_GC
Cuts at: 4024 4024
Size: 6607
Bme1580I G_kGCm'C
Cuts at: 1790 4668 5824 1790
Size: 2878 1156 2573
Fragments arranged by size:
2878 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6607
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3611 4338 5296
Size: 416 53 83 399 24 2194 727 958
Cuts at: 5296 442
Size: 1753
Fragments arranged by size:
2194 1753 958 727 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3398
Size: 6607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3765 5267 1239
Size: 2526 1502 2579
Fragments arranged by size:
2579 2526 1502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4732 5597 5895 4732
Size: 865 298 5444
Fragments arranged by size:
5444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5270 882
Size: 617 3771 2219
Fragments arranged by size:
3771 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2414 4398 493
Size: 711 1210 1984 2702
Fragments arranged by size:
2702 1984 1210 711
BsaBI GATnn'nnATC
Cuts at: 2859 2965 3801 6601 2859
Size: 106 836 2800 2865
Fragments arranged by size:
2865 2800 836 106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4414 4796 5431 275
Size: 53 83 186 936 2881 382 635 1451
Fragments arranged by size:
2881 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5040 5438 5712 5859 6270 5040
Size: 398 274 147 411 5377
Fragments arranged by size:
5377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2223 2253 1178
Size: 30 165 30 820 30 5532
Fragments arranged by size:
5532 820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3063 1186
Size: 1877 4730
BseYI C'CCAG_C
Cuts at: 5810 6306 5810
Size: 496 6111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6034
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2821 4057 4818 5846 1540
Size: 275 186 820 1236 761 1028 2301
Fragments arranged by size:
2301 1236 1028 820 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4668 4753 5824 4668
Size: 85 1071 5451
Fragments arranged by size:
5451 1071 85
BsmI GAATG_Cn'
Cuts at: 2908 3821 4118 4211 2908
Size: 913 297 93 5304
Fragments arranged by size:
5304 913 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2341 3926 4668 4753 5824 1391
Size: 399 551 1585 742 85 1071 2174
Fragments arranged by size:
2174 1585 1071 742 551 399 85
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 4496 6226 4496
Size: 1730 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3962 844
Size: 3118 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4494 1394
Size: 429 2671 3507
Fragments arranged by size:
3507 2671 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3145 5102 5276 65
Size: 1183 1897 1957 174 1396
Fragments arranged by size:
1957 1897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5251 5438 1700
Size: 110 3441 187 2869
Fragments arranged by size:
3441 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6607
BssSI C'ACGA_G
Cuts at: 4667 5679 4667
Size: 1012 5595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6607
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6607
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2696 3434 3533 3937 4304 4689 4706 6158
Size: 724 738 99 404 367 385 17 1452
Cuts at: 6158 6244 6256 6602 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 738 724 404 385 367 346
99 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3806 513
Size: 552 2741 3314
Fragments arranged by size:
3314 2741 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5868
Fragments arranged by size:
5868 683 56
BtsI GCAGTG_nn'
Cuts at: 4123 4917 4937 4123
Size: 794 20 5793
Fragments arranged by size:
5793 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DraIII CAC_nnn'GTG
Cuts at: 3050 3386 3050
Size: 336 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2666 5614 809
Size: 507 1350 2948 1802
Fragments arranged by size:
2948 1802 1350 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3439 4054 4943
Size: 54 1425 50 275 18 1609 615 889
Cuts at: 4943 6572 8
Size: 1629 43
Fragments arranged by size:
1629 1609 1425 889 615 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4054 1537
Size: 275 2242 4090
Fragments arranged by size:
4090 2242 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4537 1353
Size: 3184 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5194 5568 5714 387
Size: 1064 138 3605 374 146 1280
Fragments arranged by size:
3605 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2723 3725 3765 4670 5267 6054
Size: 393 644 447 1002 40 905 597 787
Cuts at: 6054 6322 1239
Size: 268 1524
Fragments arranged by size:
1524 1002 905 787 644 597 447 393
268 40
EcoRI G'AATT_C
Cuts at: 2758 3916 4294 2758
Size: 1158 378 5071
Fragments arranged by size:
5071 1158 378
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 5754 1678
Size: 4076 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2328 2360 2519
Size: 32 135 32 548 32 176 32 159
Cuts at: 2519 2551 2867 2887 2899 2919 3554 3586 5248
Size: 32 316 20 12 20 635 32 1662
Cuts at: 5248 5280 5322 5354 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1662 635 548 316 176 159 135
42 32 32 32 32 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2993 3951 4197 669
Size: 988 281 1055 958 246 3079
Fragments arranged by size:
3079 1055 988 958 281 246
HindIII A'AGCT_T
Cuts at: 748 2713 748
Size: 1965 4642
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KpnI G_GTAC'C
Cuts at: 2811 3932 2811
Size: 1121 5486
MfeI C'AATT_G
Cuts at: 2067 4206 2067
Size: 2139 4468
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2689 3792 5721 5905 1305
Size: 1384 1103 1929 184 2007
Fragments arranged by size:
2007 1929 1384 1103 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3441 10
Size: 54 1425 343 1609 3176
Fragments arranged by size:
3176 1609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2685 2799 2913 3046 3504 3767 3805
Size: 648 1519 114 114 133 458 263 38
Cuts at: 3805 4565 4924 5083 518
Size: 760 359 159 2042
Fragments arranged by size:
2042 1519 760 648 458 359 263 159
133 114 114 38
MspA1I CmG'CkG
Cuts at: 1606 4023 4700 5848 6093 1606
Size: 2417 677 1148 245 2120
Fragments arranged by size:
2417 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6055
NdeI CA'TA_TG
Cuts at: 387 2433 387
Size: 2046 4561
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 4054 4054
Size: 6607
NsiI A_TGCA'T
Cuts at: 3802 3802
Size: 6607
NspI r_CATG'y
Cuts at: 1132 1229 3965 5510 1132
Size: 97 2736 1545 2229
Fragments arranged by size:
2736 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6607
PciI A'CATG_T
Cuts at: 5506 5506
Size: 6607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 2957 1108
Size: 1290 559 4758
Fragments arranged by size:
4758 1290 559
PfoI T'CCnGG_A
Cuts at: 3781 3781
Size: 6607
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2223 2255 4676 4708 6189 6221 2223
Size: 32 2421 32 1481 32 2609
Fragments arranged by size:
2609 2421 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 2223 4177 2223
Size: 1954 4653
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3505 3537 3505
Size: 32 6575
PstI C_TGCA'G
Cuts at: 830 3959 830
Size: 3129 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 3666 4855 6567 3666
Size: 1189 1712 3706
Fragments arranged by size:
3706 1712 1189
SfcI C'TryA_G
Cuts at: 826 1045 2728 3955 5090 5771 5962 826
Size: 219 1683 1227 1135 681 191 1471
Fragments arranged by size:
1683 1471 1227 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3934 1872
Size: 2062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 4711 5612 5874 820
Size: 197 926 111 2657 901 262 1553
Fragments arranged by size:
2657 1553 926 901 262 197 111
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 3965 1132
Size: 2833 3774
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6607
StyI C'CwwG_G
Cuts at: 513 1065 4046 513
Size: 552 2981 3074
Fragments arranged by size:
3074 2981 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4634 4651 4804 4989 5434 325
Size: 1255 3054 17 153 185 445 1498
Fragments arranged by size:
3054 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3664 4853 6565
Size: 274 80 33 51 1443 1687 1189 1712
Cuts at: 6565 96
Size: 138
Fragments arranged by size:
1712 1687 1443 1189 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6567 6599 6567
Size: 32 6575
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2580 2580
Size: 6607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3932 1870
Size: 2062 4545
XmnI GAAnn'nnTTC
Cuts at: 2208 4736 2208
Size: 2528 4079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI